Characterization and Geographical Classification of Greek Fir Honeys Based on Physicochemical Parameters, Colour Attributes, and Volatile Compounds Using Chemometrics
The objective of the present study was to characterize commercial fir honey produced in different regions in Greece and investigate the possibility of geographical classification using data sets of physicochemical parameters, colour attributes, and volatile compounds using multivariate analysis techniques. For this purpose, 43 fir honey samples were collected during harvesting periods 2010-2013 from five different regions in Greece. Physicochemical parameters (electrical conductivity, ash, pH, moisture, free acidity, lactonic acidity, total acidity, lactonic to free acidity ratio) were determined using official methods of analysis, while colour attributes (L*, a*, b*) and browning index were determined using CIE recommendations and methodology published in the literature, respectively. Finally, volatile profiles were determined using headspace solid phase microextraction coupled to gas chromatography/mass spectrometry. Results showed that honey samples analyzed met the criteria set by regulatory standards for fir honey, and exhibited variations in physicochemical parameter values, colour attributes, and volatile profiles, according to geographical origin. On the basis of the results obtained, it is possible to classify (correct prediction 69.8%) commercial fir honeys produced in specific regions, using a data set of 21 parameters and chemometrics
Characterization of Selected Honey in South-East Nigeria: Theoretical Transla...IJEAB
With the vast honey bee species producing honey for international export and consumption in Nigeria, there is need for theoretical translation of quality assessment and characterization of honey for human consumption. The physicochemical and mineral contents of some selected honey in the five South east geopolitical states of Nigeria was performed for above mentioned application. The results were evaluated with 3D plot to identify the statistical significance of the parameters analyzed. The levels of glucose and fructose were accepted by codex alimentation standard and rejected samples B, C, and G. A correlation of similar botanical origin was demonstrated in sample B, C and G and similarly observed in their moisture content been > 21%. The pH and electrical conductivity showed no significant variation. The codex hydroxyl methyl furfural standard identified samples B, E and L to be “aged honey” or falsified honey in circulation. The 3D plot showed the significant variation of hydroxyl methyl furfural content of samples. A hypothesis was observed when the samples and previously analyzed Nigerian samples were compared; metal concentration levels of Group 1 elements > Group 2 > Transition metals in Nigerian honey and formed an identification trend.
Unifloral ajwain honey ameliorates differential inhibition of matrix metallop...HaloCantik
Free radicals lead to inflammation, which in turn could intervene several chronic diseases including cancer. The promising scientific finding for anti-cancer properties of honey is an area of great interest.
Objective
The present study was designed to investigate the in vitro biological effects (cytotoxic, and anti-inflammatory through differential inhibition of metalloproteinases and antioxidant) of unifloral Ajwain honey along with its physicochemical properties (pH, moisture, ash content, electrical conductivity, color, protein).
Materials and methods
Three Ajwain honey samples (AJ-1, AJ-2, and AJ-3) were collected from different geographical origins of Western Ghats of India. Melissopalynological analysis was carried out to confirm uniflorality. Physicochemical analysis for ash, moisture content, pH, electrical conductivity, color, and total protein was estimated. Total polyphenol, total flavonoid content, and ferric reducing ability of plasma assay were determined using appropriate methods. The cytotoxic effect was assessed against breast cancer cells (MDA-MB-231) by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the anti-inflammatory activity was evaluated by gelatin zymography of matrix metalloproteinases MMP-2 and MMP-9 proteins.
The sources and quality of iranian honeyHaloCantik
Iran is one of the largest honey-producing countries worldwide and is considered as an important source of honey for international markets. However, since Iran is not registered for honey export to Europe, the quality of Iranian honey remains unknown to European traders. As the first step in filling this gap, we analyzed 225 honey samples using palynology, sensory, nuclear magnetic resonance (NMR) and conventional physicochemical analyses as outlined by the European Union coordinated control plan. The results show that while various types of genuine unifloral honey can be harvested in Iran, 85% of collected samples were adulterated.
Bioactive compounds and antibacterial activities in crystallized honey liquef...HaloCantik
The effect of ultrasound on the crystal size, phenols, flavonoids, Maillard products and antibacterial activity of crystallized honeys was studied. Three multifloral honeys (M), one monofloral (MO) and one honeydew (HD) honey were used. Ultrasound was performed at 42 kHz for different times (0, 5, 10 and 15 min). The antibacterial activities were tested against Salmonella typhimurium, Bacillus subtilis, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus and Escherichia coli. In all honeys, the parameters analyzed had significant differences ((P < 0.05)). After 15 min of ultrasound the HD had increments of 44 mg of gallic acid/100 g of honey in phenols, and some M showed increase in flavonoids (5.64 mg of quercitin /100 g of honey) and improvement in inhibition against Salmonella typhimurium was 13.1%. In some honeys the correlation between phenols or flavonoids and antibacterial activity were significant ((P < 0.05)). No correlation was found between Maillard products and antibacterial activity. The ultrasound treatment effect on the crystal size, phenols, flavonoid, Maillard products, and antibacterial activity of crystallized honeys were different in each honey.
Quantitative determination of 20-hydroxyecdysone in methanolic extract of twi...Margareth Gallo
20-Hydroxyecdysone (20E) is effective in stimulating protein synthesis, therefore, it has been largely used as anabolic agent in several commercial
formulas. Phytochemical study of methanolic extract of twigs from Vitex polygama, used in traditional Brazilian medicine as emenagogue, yielded
a large quantity of 20E. This finding led us to developing and validating a simple and reliable method to determine 20E in the surveyed extract.
Chromatographic separation of 20E was achieved on a phenyl-hexyl-based column using reversed elution mode. Extract was cleaned-up by solid
phase extraction employing C18 cartridge, and an absolute recovery of 97% was acquired. External standard and standard addition calibration
graphs were obtained and good linearity was accomplished (r > 0.999 for both curves). The limit of quantification and detection were determined.
The results for accuracy fell within the −5 to +7% range.
The Vine is an area within the WBWE, conceived to encourage new businesses that evolve around wine and vine industries. Using every resource, and expanding every possibility, such an initiative shows the great potential that can be developed in the sector.
Food Colour & Quality Laboratory, Department of Nutrition & Food Science, Universidad de Sevilla.
Further information about "The Vine Science Award" on http://www.worldbulkwine.com/thevine/ing/posters.html
Qualitative Evaluation and Wound Healing Potential of Selected Medicinal Plan...BRNSS Publication Hub
Objective: The aim of the paper was to assess the wound healing potential of three medicinal plants using the excision wound healing model on albino rat. Materials and Methods: Soxhlet extraction method was utilized for the partition of the constituent of interest. Qualitative analysis and phytochemical screening were performed for the detection of tannins, alkaloids, resins, flavonoids, glycosides, steroids, proteins, carbohydrates, and amino acids. Three plants extract used for the ointment formulation and prepared by the addition of extract of Artocarpus heterophyllus, Murraya koenigii, and Punica granatum in polyethylene glycol ointment base. Three ointment formulations and six extracts with 5% and 10% extract concentration have been used. Excision wound rat model utilized for the wound healing potential. Results and Conclusion: All three plants, including A. heterophyllus Lam., M. koenigii Linn., and P. granatum Linn. extracted for the active constituent. The pharmacological evaluation on the excision wound healing model suggested that Group-I animals showed 52.09% of healing, whereas povidone-iodine treated animals showed 100.00% healing. On the other hand, the ointment formulation treated F-1 showed 96.47% of wound healing, F-2 showed 97.68% healing, and F-3 showed 99.11% healing. The overall healing results can be represented as following: Control <MKL5 <MKL10 <PGB5 <PGB10 <AHP5 <AHP10 <F-1 <F-2 <F-3 <Standard. F3 ointment formulation is better than the F2 and F1 formulation in wound healing potential as compared to others. Discussion: These studies have indicated that ointment formulations of A. heterophyllus, M. koenigii, P. granatum have been utilized for wound healing potential and it is safer for topical application. Excision wound healing model suggested that the three individual plant extract has shown the wound healing potential, although the prepared ointment formulations F3 have best and synergistic action than the individual. The ointment formulations containing plant extracts in 10% amount have better wound healing potential.
Characterization of Selected Honey in South-East Nigeria: Theoretical Transla...IJEAB
With the vast honey bee species producing honey for international export and consumption in Nigeria, there is need for theoretical translation of quality assessment and characterization of honey for human consumption. The physicochemical and mineral contents of some selected honey in the five South east geopolitical states of Nigeria was performed for above mentioned application. The results were evaluated with 3D plot to identify the statistical significance of the parameters analyzed. The levels of glucose and fructose were accepted by codex alimentation standard and rejected samples B, C, and G. A correlation of similar botanical origin was demonstrated in sample B, C and G and similarly observed in their moisture content been > 21%. The pH and electrical conductivity showed no significant variation. The codex hydroxyl methyl furfural standard identified samples B, E and L to be “aged honey” or falsified honey in circulation. The 3D plot showed the significant variation of hydroxyl methyl furfural content of samples. A hypothesis was observed when the samples and previously analyzed Nigerian samples were compared; metal concentration levels of Group 1 elements > Group 2 > Transition metals in Nigerian honey and formed an identification trend.
Unifloral ajwain honey ameliorates differential inhibition of matrix metallop...HaloCantik
Free radicals lead to inflammation, which in turn could intervene several chronic diseases including cancer. The promising scientific finding for anti-cancer properties of honey is an area of great interest.
Objective
The present study was designed to investigate the in vitro biological effects (cytotoxic, and anti-inflammatory through differential inhibition of metalloproteinases and antioxidant) of unifloral Ajwain honey along with its physicochemical properties (pH, moisture, ash content, electrical conductivity, color, protein).
Materials and methods
Three Ajwain honey samples (AJ-1, AJ-2, and AJ-3) were collected from different geographical origins of Western Ghats of India. Melissopalynological analysis was carried out to confirm uniflorality. Physicochemical analysis for ash, moisture content, pH, electrical conductivity, color, and total protein was estimated. Total polyphenol, total flavonoid content, and ferric reducing ability of plasma assay were determined using appropriate methods. The cytotoxic effect was assessed against breast cancer cells (MDA-MB-231) by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the anti-inflammatory activity was evaluated by gelatin zymography of matrix metalloproteinases MMP-2 and MMP-9 proteins.
The sources and quality of iranian honeyHaloCantik
Iran is one of the largest honey-producing countries worldwide and is considered as an important source of honey for international markets. However, since Iran is not registered for honey export to Europe, the quality of Iranian honey remains unknown to European traders. As the first step in filling this gap, we analyzed 225 honey samples using palynology, sensory, nuclear magnetic resonance (NMR) and conventional physicochemical analyses as outlined by the European Union coordinated control plan. The results show that while various types of genuine unifloral honey can be harvested in Iran, 85% of collected samples were adulterated.
Bioactive compounds and antibacterial activities in crystallized honey liquef...HaloCantik
The effect of ultrasound on the crystal size, phenols, flavonoids, Maillard products and antibacterial activity of crystallized honeys was studied. Three multifloral honeys (M), one monofloral (MO) and one honeydew (HD) honey were used. Ultrasound was performed at 42 kHz for different times (0, 5, 10 and 15 min). The antibacterial activities were tested against Salmonella typhimurium, Bacillus subtilis, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus and Escherichia coli. In all honeys, the parameters analyzed had significant differences ((P < 0.05)). After 15 min of ultrasound the HD had increments of 44 mg of gallic acid/100 g of honey in phenols, and some M showed increase in flavonoids (5.64 mg of quercitin /100 g of honey) and improvement in inhibition against Salmonella typhimurium was 13.1%. In some honeys the correlation between phenols or flavonoids and antibacterial activity were significant ((P < 0.05)). No correlation was found between Maillard products and antibacterial activity. The ultrasound treatment effect on the crystal size, phenols, flavonoid, Maillard products, and antibacterial activity of crystallized honeys were different in each honey.
Quantitative determination of 20-hydroxyecdysone in methanolic extract of twi...Margareth Gallo
20-Hydroxyecdysone (20E) is effective in stimulating protein synthesis, therefore, it has been largely used as anabolic agent in several commercial
formulas. Phytochemical study of methanolic extract of twigs from Vitex polygama, used in traditional Brazilian medicine as emenagogue, yielded
a large quantity of 20E. This finding led us to developing and validating a simple and reliable method to determine 20E in the surveyed extract.
Chromatographic separation of 20E was achieved on a phenyl-hexyl-based column using reversed elution mode. Extract was cleaned-up by solid
phase extraction employing C18 cartridge, and an absolute recovery of 97% was acquired. External standard and standard addition calibration
graphs were obtained and good linearity was accomplished (r > 0.999 for both curves). The limit of quantification and detection were determined.
The results for accuracy fell within the −5 to +7% range.
The Vine is an area within the WBWE, conceived to encourage new businesses that evolve around wine and vine industries. Using every resource, and expanding every possibility, such an initiative shows the great potential that can be developed in the sector.
Food Colour & Quality Laboratory, Department of Nutrition & Food Science, Universidad de Sevilla.
Further information about "The Vine Science Award" on http://www.worldbulkwine.com/thevine/ing/posters.html
Qualitative Evaluation and Wound Healing Potential of Selected Medicinal Plan...BRNSS Publication Hub
Objective: The aim of the paper was to assess the wound healing potential of three medicinal plants using the excision wound healing model on albino rat. Materials and Methods: Soxhlet extraction method was utilized for the partition of the constituent of interest. Qualitative analysis and phytochemical screening were performed for the detection of tannins, alkaloids, resins, flavonoids, glycosides, steroids, proteins, carbohydrates, and amino acids. Three plants extract used for the ointment formulation and prepared by the addition of extract of Artocarpus heterophyllus, Murraya koenigii, and Punica granatum in polyethylene glycol ointment base. Three ointment formulations and six extracts with 5% and 10% extract concentration have been used. Excision wound rat model utilized for the wound healing potential. Results and Conclusion: All three plants, including A. heterophyllus Lam., M. koenigii Linn., and P. granatum Linn. extracted for the active constituent. The pharmacological evaluation on the excision wound healing model suggested that Group-I animals showed 52.09% of healing, whereas povidone-iodine treated animals showed 100.00% healing. On the other hand, the ointment formulation treated F-1 showed 96.47% of wound healing, F-2 showed 97.68% healing, and F-3 showed 99.11% healing. The overall healing results can be represented as following: Control <MKL5 <MKL10 <PGB5 <PGB10 <AHP5 <AHP10 <F-1 <F-2 <F-3 <Standard. F3 ointment formulation is better than the F2 and F1 formulation in wound healing potential as compared to others. Discussion: These studies have indicated that ointment formulations of A. heterophyllus, M. koenigii, P. granatum have been utilized for wound healing potential and it is safer for topical application. Excision wound healing model suggested that the three individual plant extract has shown the wound healing potential, although the prepared ointment formulations F3 have best and synergistic action than the individual. The ointment formulations containing plant extracts in 10% amount have better wound healing potential.
No other food products focus more consumer attention than those prepared for consumption by children. Identification and quantification of natural sugars were investigated in baby food products by mid-IR Fourier Transform Infrared (FTIR) spectroscopy. Using Horizontal Attenuated Total Reflectance (HATR), neat baby food samples were analyzed without need for extensive sample preparation. For more info, go to ssi.shimadzu.com and follow Shimadzu on Twitter @shimadzussi. Thanks for viewing!
formulation, acceptability and storage stability of appetized ginger plum lea...IJEAB
Appetized ginger plum leather was prepared by using different combinations of ginger and plum pulp with varying concentrations of appetizing mixture. The TSS of ginger and plum pulp were raised to 25oB by adding sugar and 1.0 to 2.5% appetizing mixture, followed by drying thin layers in dehydrator (55±2oC) to 12-14% moisture content. The standardization of most palatable recipe was done by evaluating sensory properties and highest score was obtained by ginger: plum (50:50) and 1.5% appetizing mixture. The appetized leather contained comparatively higher amount of ascorbic acid (13.16mg/100g), total phenols (55.89mg/100g) and antioxidant activity (72.94%). The leather was found most stable when packaged in laminated aluminium pouches during storage. The leather did not exhibit appreciable changes in titratable acidity, ascorbic acid, total sugars, phenols and antioxidant activity after 6 months. Thus the appetized ginger plum leather can be stored under ambient storage after packing in aluminium laminated pouches.
Isolation and Purification of Secoisolariciresinoldiglucoside oligomers (Lign...IOSR Journals
The present study aimed to extract and purify the compound of Secoisolariciresinoldiglucoside
oligomers (lignan) from flax seed (Linumusitatissimum) and its antioxidant activity. The Lignan was extracted
by solvents which gave the best results were ethanol : 1,4 dioxane (1:1, v:v).SDG release after alkaline
hydrolysisby using a methanolicNaOH , 20 mM, pH=8 at 50 ºC.followed by using following chromatographic
techniques: Liquid-liquid, Sephadex LH-20 column chromatography, thin layer chromatographic (TLC), high
performance liquid chromatographic (HPLC) and Fourier Transform Infra-Red(FTIR) . The EC50 values of
Pure lignan extract (9 μg/ml) was shown possess DPPH radical scavenging activity compared to reference
substances BHT and vitamin C (EC50= 3 and 4.2 μg/ml) respectively, and this was higher than partial pure
lignan component (EC50= 25.5 μg/ml).The total phenolic content of the pure lignanwas higher than partial
pure lignan which gave 22.312 and 14.85 g/ml respectively.
Evaluation of the Antioxidant Activities of Organic Extracts from Ammodaucus ...CrimsonAlternativemedicine
Aromatic and medicinal plants are a good source of natural preparations containing effective bioactive compounds which can be used for different applications. This work aims to evaluate the antioxidant activity of some organic extracts of Ammodaucus leucotrichus Coss & Dur fruit part. The whole plant was collected from the region of Beni Abbas (Bechar-Algeria). Five organic extracts were obtained and the evaluation of the antioxidant activity was performed by six conventional methods. Polar organic extracts exhibited more antioxidant power then non polar extracts. The level of phenolic compounds was moderate in all extracts. The investigation of the antioxidant activity of organic extracts from fruit part of Ammodaucus leucotrichusrevealed a moderate activity tested by six conventional methods.
For more open access journals in Crimson Publishers please click on link: https://crimsonpublishers.com/
For more articles in open access Complementary Medicine journals please click on link: https://crimsonpublishers.com/acam/
Physiochemical Screening of Carica papaya Leaves with Specific Reference to T...BRNSS Publication Hub
Carica papaya is made to develop pharmacognostical characters of leaf with their morphological, microscopical, and physical characters including histochemical analysis. Morphological evaluation as color, odor, taste, size, shape, surface, and powder microscopy of plant shows the presence of endosperm cell which is polygonal in shape and contains aleurone grains and oil droplet, cell of testa, yellow coloring matter, and starch grains. Quantitative leaf microscopy to determine palisade ratio, stomata index, and vein-islet number is carried out. Peels are removed mechanically through epidermal peeling off and stomatal index (SI) is calculated. The vein-islet number, vein termination number, and palisade ratio of lamina are determined according to the standard method. We prepared the extracts of plant with different solvents for determining the different extractive values by maceration, Soxhlet extraction, successive extraction process, and determination of ash values, pH value, moisture content, and phytochemical screening to show the presence of carbohydrates, phenolic compounds, flavonoids, alkaloids, proteins, saponins, and lipids in the drug extract and fluorescence analysis in different solvent. Analysis of pesticide residues, aflatoxin, and heavy metals are also performed
Flavoring and medicinal values of the yellow pigment produced by Monascus rub...Premier Publishers
The Thin Layer Chromatographic analysis (TLC) of the crud red pigment extract of M. ruber 4066 cultivated on the malt static medium well developed into three separate bands includes red, orange and yellow pigmented bands. Sixty four volatile metabolites are detected by GC/MS analysis which gave yellow pigment high flavoring value. The detected metabolites are classified, chemical and physical properties are characterized, and their uses are reported. The detected metabolites including fourteen aliphatic metabolites, twenty nine aromatic metabolites and terpenoids, thirteen nitrogenous metabolites and also five heterocyclic nitrogenous thiols metabolites are detected. Also they are classified into thirteen volatile and aroma groups includes one metabolite from the each "alcohol, acid, aldehyde, amine, furan and mercapto", two phenols, three amides, four from each "indoles and ketones", twelve terpenoids, fourteen alkanes & alkenes, sixteen esters and three metabolites are unknown for me.
Nutritional Profile and Physicochemical Properties of Peach Varieties in Ethi...AJSERJournal
Nutritional and functional characteristics of fruits are related to their quality and are influenced by
genotype and ripening stage, and by environmental conditions and orchard management practices. The purpose of this
research was to test nutritional profile and selected physicochemical properties of different improved and adopted
peach varieties in Ethiopia and comparative study among varieties and between study varieties and standard
reference. The result got show that improved peach varieties greater amount of ash (4.3-5.51%), protein (4.34-6.05%),
fat (0.097-1.386%) and fiber (2.87-4.611%) than standard reference (0.263, 1.423, 0.427, and 1.20%, in the
aforementioned order) but lower in carbohydrate (84.411-89.90%) than standard of (96.678%). The mineral content
higher in K (0.48-1.182%), Ca (0.162-0.565 %), Mg (0.037-0.066%), Fe (16.33-159.2%) and Zn (2.43-8.84%) than
standard reference (0.942, 0.051, 0.071, 16.32, 3.213%), respectively except Na, Mg, Cu and Sulfur while the fruit
quality was revealed low moisture content which less water and medium vitamin C (3.99-5.55%) and TSS (8.36-
14.31%). We observed variation in proximate composition and mineral content among sixteen peach varieties.
Greek Honeys Exhibit Phenolic Acids with Antiatherogenic, Anticancer and Anti...Bee Healthy Farms
Greek honeys are rich in phenolic acids, in particular protocatechuic and p-hydroxybenzoic acid and exhibit significant antioxidant, anticancer and antiatherogenic activities which may be attributed, at least in part, to their phenolic acid content.
Submerged fermentation of laccase producing Streptomyces chartreusis using bo...IOSR Journals
Response surface methodology was engaged for the optimization of diverse nutritional and physical parameters for laccase production by Streptomyces chartreusis strain NBRC 12753 in the submerged fermentation process. Screening of production parameters was executed using Plackett–Burman design and the variables with statistically momentous effects on laccase production were recognized. Variables such as Cupric sulphate, Pyrogallol and Yeast extract were selected for further optimization studies using Box-Behnken design. The multiple regression coefficients (R2) had a value of 0.9606, indicating that the model could explain up to 96.06 % of the variability of the response. This methodology facilitated analysis of the experimental data to establish the optimum conditions for the process and understand the contribution of individual factors to evaluate the response under optimal conditions. Thus application of Box-Behnken approach appears to have potential usage in process application.
No other food products focus more consumer attention than those prepared for consumption by children. Identification and quantification of natural sugars were investigated in baby food products by mid-IR Fourier Transform Infrared (FTIR) spectroscopy. Using Horizontal Attenuated Total Reflectance (HATR), neat baby food samples were analyzed without need for extensive sample preparation. For more info, go to ssi.shimadzu.com and follow Shimadzu on Twitter @shimadzussi. Thanks for viewing!
formulation, acceptability and storage stability of appetized ginger plum lea...IJEAB
Appetized ginger plum leather was prepared by using different combinations of ginger and plum pulp with varying concentrations of appetizing mixture. The TSS of ginger and plum pulp were raised to 25oB by adding sugar and 1.0 to 2.5% appetizing mixture, followed by drying thin layers in dehydrator (55±2oC) to 12-14% moisture content. The standardization of most palatable recipe was done by evaluating sensory properties and highest score was obtained by ginger: plum (50:50) and 1.5% appetizing mixture. The appetized leather contained comparatively higher amount of ascorbic acid (13.16mg/100g), total phenols (55.89mg/100g) and antioxidant activity (72.94%). The leather was found most stable when packaged in laminated aluminium pouches during storage. The leather did not exhibit appreciable changes in titratable acidity, ascorbic acid, total sugars, phenols and antioxidant activity after 6 months. Thus the appetized ginger plum leather can be stored under ambient storage after packing in aluminium laminated pouches.
Isolation and Purification of Secoisolariciresinoldiglucoside oligomers (Lign...IOSR Journals
The present study aimed to extract and purify the compound of Secoisolariciresinoldiglucoside
oligomers (lignan) from flax seed (Linumusitatissimum) and its antioxidant activity. The Lignan was extracted
by solvents which gave the best results were ethanol : 1,4 dioxane (1:1, v:v).SDG release after alkaline
hydrolysisby using a methanolicNaOH , 20 mM, pH=8 at 50 ºC.followed by using following chromatographic
techniques: Liquid-liquid, Sephadex LH-20 column chromatography, thin layer chromatographic (TLC), high
performance liquid chromatographic (HPLC) and Fourier Transform Infra-Red(FTIR) . The EC50 values of
Pure lignan extract (9 μg/ml) was shown possess DPPH radical scavenging activity compared to reference
substances BHT and vitamin C (EC50= 3 and 4.2 μg/ml) respectively, and this was higher than partial pure
lignan component (EC50= 25.5 μg/ml).The total phenolic content of the pure lignanwas higher than partial
pure lignan which gave 22.312 and 14.85 g/ml respectively.
Evaluation of the Antioxidant Activities of Organic Extracts from Ammodaucus ...CrimsonAlternativemedicine
Aromatic and medicinal plants are a good source of natural preparations containing effective bioactive compounds which can be used for different applications. This work aims to evaluate the antioxidant activity of some organic extracts of Ammodaucus leucotrichus Coss & Dur fruit part. The whole plant was collected from the region of Beni Abbas (Bechar-Algeria). Five organic extracts were obtained and the evaluation of the antioxidant activity was performed by six conventional methods. Polar organic extracts exhibited more antioxidant power then non polar extracts. The level of phenolic compounds was moderate in all extracts. The investigation of the antioxidant activity of organic extracts from fruit part of Ammodaucus leucotrichusrevealed a moderate activity tested by six conventional methods.
For more open access journals in Crimson Publishers please click on link: https://crimsonpublishers.com/
For more articles in open access Complementary Medicine journals please click on link: https://crimsonpublishers.com/acam/
Physiochemical Screening of Carica papaya Leaves with Specific Reference to T...BRNSS Publication Hub
Carica papaya is made to develop pharmacognostical characters of leaf with their morphological, microscopical, and physical characters including histochemical analysis. Morphological evaluation as color, odor, taste, size, shape, surface, and powder microscopy of plant shows the presence of endosperm cell which is polygonal in shape and contains aleurone grains and oil droplet, cell of testa, yellow coloring matter, and starch grains. Quantitative leaf microscopy to determine palisade ratio, stomata index, and vein-islet number is carried out. Peels are removed mechanically through epidermal peeling off and stomatal index (SI) is calculated. The vein-islet number, vein termination number, and palisade ratio of lamina are determined according to the standard method. We prepared the extracts of plant with different solvents for determining the different extractive values by maceration, Soxhlet extraction, successive extraction process, and determination of ash values, pH value, moisture content, and phytochemical screening to show the presence of carbohydrates, phenolic compounds, flavonoids, alkaloids, proteins, saponins, and lipids in the drug extract and fluorescence analysis in different solvent. Analysis of pesticide residues, aflatoxin, and heavy metals are also performed
Flavoring and medicinal values of the yellow pigment produced by Monascus rub...Premier Publishers
The Thin Layer Chromatographic analysis (TLC) of the crud red pigment extract of M. ruber 4066 cultivated on the malt static medium well developed into three separate bands includes red, orange and yellow pigmented bands. Sixty four volatile metabolites are detected by GC/MS analysis which gave yellow pigment high flavoring value. The detected metabolites are classified, chemical and physical properties are characterized, and their uses are reported. The detected metabolites including fourteen aliphatic metabolites, twenty nine aromatic metabolites and terpenoids, thirteen nitrogenous metabolites and also five heterocyclic nitrogenous thiols metabolites are detected. Also they are classified into thirteen volatile and aroma groups includes one metabolite from the each "alcohol, acid, aldehyde, amine, furan and mercapto", two phenols, three amides, four from each "indoles and ketones", twelve terpenoids, fourteen alkanes & alkenes, sixteen esters and three metabolites are unknown for me.
Flavoring and medicinal values of the yellow pigment produced by Monascus rub...
Similar to Characterization and Geographical Classification of Greek Fir Honeys Based on Physicochemical Parameters, Colour Attributes, and Volatile Compounds Using Chemometrics
Nutritional Profile and Physicochemical Properties of Peach Varieties in Ethi...AJSERJournal
Nutritional and functional characteristics of fruits are related to their quality and are influenced by
genotype and ripening stage, and by environmental conditions and orchard management practices. The purpose of this
research was to test nutritional profile and selected physicochemical properties of different improved and adopted
peach varieties in Ethiopia and comparative study among varieties and between study varieties and standard
reference. The result got show that improved peach varieties greater amount of ash (4.3-5.51%), protein (4.34-6.05%),
fat (0.097-1.386%) and fiber (2.87-4.611%) than standard reference (0.263, 1.423, 0.427, and 1.20%, in the
aforementioned order) but lower in carbohydrate (84.411-89.90%) than standard of (96.678%). The mineral content
higher in K (0.48-1.182%), Ca (0.162-0.565 %), Mg (0.037-0.066%), Fe (16.33-159.2%) and Zn (2.43-8.84%) than
standard reference (0.942, 0.051, 0.071, 16.32, 3.213%), respectively except Na, Mg, Cu and Sulfur while the fruit
quality was revealed low moisture content which less water and medium vitamin C (3.99-5.55%) and TSS (8.36-
14.31%). We observed variation in proximate composition and mineral content among sixteen peach varieties.
Greek Honeys Exhibit Phenolic Acids with Antiatherogenic, Anticancer and Anti...Bee Healthy Farms
Greek honeys are rich in phenolic acids, in particular protocatechuic and p-hydroxybenzoic acid and exhibit significant antioxidant, anticancer and antiatherogenic activities which may be attributed, at least in part, to their phenolic acid content.
Submerged fermentation of laccase producing Streptomyces chartreusis using bo...IOSR Journals
Response surface methodology was engaged for the optimization of diverse nutritional and physical parameters for laccase production by Streptomyces chartreusis strain NBRC 12753 in the submerged fermentation process. Screening of production parameters was executed using Plackett–Burman design and the variables with statistically momentous effects on laccase production were recognized. Variables such as Cupric sulphate, Pyrogallol and Yeast extract were selected for further optimization studies using Box-Behnken design. The multiple regression coefficients (R2) had a value of 0.9606, indicating that the model could explain up to 96.06 % of the variability of the response. This methodology facilitated analysis of the experimental data to establish the optimum conditions for the process and understand the contribution of individual factors to evaluate the response under optimal conditions. Thus application of Box-Behnken approach appears to have potential usage in process application.
In Ayurveda, the leaf juice of Adhatoda vasica, a shrub native to Asia is incorporated in
many traditional herbal formulations. However, suitable solvent and a suitable extraction
method for phytochemical profiling are not well established, and there is no published mass
spectra structural interpretation of the identified compounds. This has caused a few
problems in herbal formulation research due to the bias derived from different extraction
methods. Therefore, this study used polar and non polar extraction for phytochemical
analysis on Adhatoda vasica, aiming to assess the potential impact of different solvents. This
study included extractive value, total phenol and alkaloid content of the leaves in different
preparations. Gas Chromatography coupled with Mass Spectrometry (GC-MS) was used to
study the phytochemical profile of different solvents. Significant differences were observed in
all the parameters such as extract yield, total phenol, total alkaloid and phytochemical
composition. The ethanol extract stood out most for effective extraction of phytochemicals,
especially for the alkaloids. The results highlight the necessity for comparative analyses of
chemical composition in different solvent extractions and careful choice and validation of
analytical methodology in herbal formulation research.
composición química de semillas y pulpa de pasiflora edulis
Similar to Characterization and Geographical Classification of Greek Fir Honeys Based on Physicochemical Parameters, Colour Attributes, and Volatile Compounds Using Chemometrics (20)
Effect of Different Weed Control Practices On Proximate Composition, Nutrient...IOSRJAVS
A field experiment was conducted during 2004 and 2005 cropping seasons to determine effect of different weed control practices on proximate composition, nutrient concentration and nutrient uptake of maize (Zea maysL.) at the Teaching and Research Farm of Ambrose Alli University, Ekpoma, Nigeria. Seven treatments were used for the experiment, viz no weeding (control), Primextra (3.0kg a.i./ha), mulching (wood shavings), one- hoe weeding at 3 weeks after planting (WAP), two hoe- weedings at 3 and 7 WAP, cover cropping with melon minus hoe-weeding, cover-cropping with melon plus one hoe-weeding at 3WAP. The seven treatments were laid out in randomized complete block design with four replicates. Results showed that mulched plot with wood shavings had the lowest weed density and highest proximate composition, grain nutrient concentration, ear leaf concentration and nutrient uptake followed by Primextra treated plot .The study recommends that small holder farmers to adopt mulching technique with wood shaving to improve the nutritional quality of maize grain since it does not involve any technical rigor besides signifying a non-chemical weed control.
Physiological Quality of Bean Seeds Related To Azotobacter spp. InoculationIOSRJAVS
Research aimed at improving the quality of crops. The results obtained with seed coating were very influential with regard to fertilization and disease resistance. Nitrogen fertilization increases costs in agricultural production and the loss of fertile lands, altering the natural conditions and has negative consequences for the microorganisms (MO), which regulate the balance between quality of crops and soils. The biological nitrogen fixation (BNF) consists on MO work which provide nitrogen in soil and secrete substances that promote plant growth, these can be made by inoculation of seeds. The germination rate, emergency and vigor are an indicator that determines the productivity and physiological quality of a plant variety. The germination rate, number of emerged seeds per pot in favorable field conditions and vigor by accelerated aging test were evaluated. The seed treatments were: the seed inoculation with Azotobacter spp., Immersion in nutrient broth without bacteria, sterilization and zero handling. The experiment has shown that inoculation of common bean seeds with Azotobacter spp. does not adversely affect germination, emergency or vigor and stimulate the development of abnormal seedlings.
Prevalence and Antibiotic sensitivity pattern of Salmonella isolates from mil...IOSRJAVS
The study was conducted to determine the presence of Salmonella in retail milk products and water reservoirs in Maiduguri, Nigeria. A total of 150 samples were collected using convenient sampling technique. One hundred samples; fifty each of full cream milk or Kindirmo (n=50) and Skimmed milk or Nono (n=50) were collected from Bulumkutu and Monday market retail sellers. Furthermore, of the fifty samples obtained from the two different locations, twenty five samples each of kindirmo and nono were collected. The samples were aseptically kept in sterile plastic bags. Additionally, fifty water samples were collected from reservoirs within the University campus. They were processed according to standard bacteriological protocols followed by Gram’s staining and biochemical test; Triple sugar iron test, citrate and urease. The Salmonella isolates were further subjected to ten different antibiotics to determine their sensitivity. The overall prevalence of Salmonella in milk samples was found to be 10.00%, while the total prevalence of Salmonella from water sample was 40.00%. Of the fifteen isolates tested, resistance to Amoxicillin, Ceftriaxone and Erythromycin was 100.00%, Gentamicin had 80.00% and Cotrimoxazole was 53.33%, whereas Gentamycin, Ceftriaxone and Amoxicillin displayed 100.00% sensitivity. Moderate sensitivity of 53.33% to Streptomycin and Pefloxacin, 46.67% to Ciprofloxacin and 86.67% sensitivity to Ofloxacin was found in this study. Antibiotic resistance is associated with frequent usage both in livestock and humans as they are commonly available. Adequate sanitary measures should be ensured in milk processing and use of water reservoirs. Antibiotics should be used based on their antibiogram pattern. Prudent use of antibiotics is essential and its continuous use as growth promoters should be discouraged, as this may result to failure in the treatment of Salmonella-associated diseases due to resistance. Disease surveillance programmes should be established as a means for curtailing salmonellosis.
Molecular Detection and Therapeutic Management of Feline MycoplasmosisIOSRJAVS
Mycoplasma haemofelis and Candidatus Mycoplasma haemominutum (formerly known as Haemobartonellafelis) are the causes of hemotropic mycoplasmosis in cats. The parasites attach to the surface of the red blood cell, and have the potential to cause severe alterations of the cell’s shape, resulting in anaemia. A three-year-old tom cat was presented in University Veterinary Hospital with symptoms of lethargy, reduced appetite and fever for past 3 days. Clinical examination revealed increased temperature(103º – 105º F), blanched mucous membranes and lymphadenopathy. Upon peripheral blood smear examination small coccoid organisms could be noticed in the periphery of the RBCs. Hematobiochemical examination revealed anaemia, thrombocytopaenia and decreased haematocrit values. The blood samples were subjected to DNA extraction and followed by Polymerase Chain Reaction which confirmed mycoplasmosis due to Mycoplasma haemofelis. The animal was treated with intravenous administration of oxytetracycline@ 10mg/kg BW for five days along with prednisolone and vitamin supplements. Uneventful clinical recovery was noticed 7 days post therapy.
Inspection of the imported food products and internally produced food product...IOSRJAVS
This research had done on Export and Internal products which use by Afghan people. Tests on eggs showed that the internal eggs have better condition because of good weight, shell size, Albumin and yolk, and also there is no report of Salmonella. Tests on milk showed that the internal milk is good at high percentage of fat, Protein, Carbohydrates but there is always adulteration in these milks like adding water and starch. The research on water showed that there are hardness, No3, No2, P, some kinds of Bacteria but no Arsenic in tap, well and mineral water.In meat test we had found that, both import frozen meat and daily slaughter had the same condition of total bacteria and Coliforms but the percentage was normal, with no Salmonella. Results showed that import apple had high Bricks in refract meter than Herati apple.Tests of sausage and tuna products of our country and the external samples had no coliforms but high percentage of total bacteria.
Agro-Economic Benefits of Weed Biomass and Crop Residue in Maize Production S...IOSRJAVS
The climatic conditions of coastal Kenya favour rapid weed growth, leading to the accumulation of large biomass of weeds between cropping seasons. Smallholder farmers in the region usually slash and remove the weed biomass and crop residue from their farms during land preparation in order to facilitate easy planting. The impact of such practice on the production of maize has not been assessed. The aim of this study was to determine the effect of the farmers’ practice on the performance of maize and fertilizer requirement. Three methods of managing weed biomass and crop residue (removal from field, incorporation into soil, or use as surface mulch) and five fertilizer rates (60 kg N ha-1 , 20 kg P ha-1 , 30 kg N ha-1 , 10 kg P ha-1 , and no fertilizer application) were evaluated. Removal of weed biomass and crop residue from the field led to 20-26% loss in grain yield and reduced the returns to labour by 41-51%.There was no response to applied P where weed biomass and crop residue had been incorporated into soil. Efforts should therefore be made to educate farmers on the advantages of retaining weed biomass and crop residue on their farms as they prepare land for subsequent crops.
Mutagenic Action of Sodium Azide on Germination and Emergence in Landraces of...IOSRJAVS
Phaseolus vulgaris (Common beans) landraces are one of the most cultivated legumes for human consumption on the montaneclimate of the Jos Plateau and is renowned for the production of reasonable quantities of the beans in Africa.Common beans is rich in fiber, micronutrients, minerals and has strong medicinal value. Despite its potential for improving nutrition and poverty reduction, it is highly under-utilized and research efforts to improve the genotypes of the plant found in this area for better productivity are few. A study was thus carried out to access the germination and emergence responses of three landraces of Phaseolus vulgaris (pinto, red kidney and navy) after treatment with sodium azide. Four doses of sodium azide were applied in concentrations of 0.1M, 0.04M, 0.03M and 0.02M for each landrace for both germination and emergence experiments. The results showed that at doses 0.02M and 0.03M, the pinto indicated significantly (p<0.05) higher mean germination which surpassed the control. All sodium azide doses in the red kidney and pinto had reduced mean germination. Sodium azide reduced the mean emergence of pinto, red kidney and navy at all the doses of sodium azide. The lethality of sodium azide was highest in navy and lowest in pinto. The decrease in germination and emergence was not dose related especially in red kidney and navy. The best response to the mutagen was observed in pinto with higher germination and the least percentage lethality. Sodium azide effectiveness and efficiency were strongest at the 0.1M dose of the mutagen.
The Use of in vitro Gas Production Technique as an Index of the Nutritive Val...IOSRJAVS
: The in vitro gas production technique was used to predict rumen fermentable organic matter, gas production kinetics, organic matter digestibility as well as metabolizable energy were evaluated in green shoot, leaves, fruits flesh and seed cakes of Ziziphus spina-christi tree. A gas production was measured by incubating samples in buffered rumen fluid from cannulated steer for 72 h. Total gas production was recorded at 0,3, 6, 9, 12, 24, 48, and 72 h of incubation periods and kinetics of gas production was described. The chemical analysis of these Ziziphus spina-christi parts, showed that, Leaves contained high protein (14.77±0.23g/kg) and green shoot less protein (8.03±0.15g/kg),however, high ash content was observed in green shoot (10.03±0.07g/kg) compare to other parts. seed cakes has the highest crude fibre content in comparison to other parts (32.46±0.01),while fruits flesh contained the highest ether extract(72.39±0.03).The maximum gas volume was highest for fruits flesh followed by seed cakes, green shoot and leaves after 24hr of incubation. In this study flesh had a significantly higher (P<0.05) gas production from rapid soluble fraction (a) than the other parts, while Ziziphus spina-christi leaves showed the highest gas production from slowly degradable fraction (b) .Organic matter digestibility range was (51.90– 43.79%) and Metabolisable energy was found to be (9.16– 6.74MJ/kgDM) in the flesh and green shoot, respectively. It was concluded that, green shoots, leaves, fruit flesh and seed cake of Ziziphus spina-christi have the potential to be used as protein, energy and mineral supplements for ruminants especially during the dry season
Epithelial reticular Cells of Egyptian Water buffalo (Bosbubalis)IOSRJAVS
Thymus obtained from10 clinically healthy Egyptian water male buffalo (Bosbubalis). Their ages ranged between 2to 3 years old. The thymus of Egyptian water buffalo was divided by septa into many lobules; each lobule had outer, cortex and inner medulla. The epithelial reticular cells were classified according to their staining affinity, morphology and position into 4 types, three were found within the cortex and one in the medulla. In cortex epithelia reticular cells type I (ER 1) had elongated nucleus of irregular or triangular shape with marginal heterochromatin clumps along the nuclear membrane. Epithelial reticular cells type II(ER II) were irregular in shape with pale stained cytoplasm and the nuclei were large and spherical having euchromatin. Epithial reticular cells type III(ERIII) were characterized by large nucleus of irregular shape having marginal heterochromatin .In medulla epithelial reticular (ER) cells of type IV, they were the largest epithelial cells forming the unique elements of the thymic medulla (Hassall's corpuscles)). Cytoplasm was occupied by many small vacuoles ,very large vacuole appeared in the cytoplasm pushed the nucleus into one side.
The Development Strategy for Community Empowerment in the Framework of Fire P...IOSRJAVS
Land and forest fire disasters occur every year. Such conditions lead to disaster smog that many negative impact on society. Limited access to information community, especially around the area of Coconut Plantation potentially cause many problems, such as reduced household income caused by smog, the highaffected community respiratory tract infections as well as conflict disharmony social relationships that exist in society. Mistakes in communicating an information, could lead to uncertainty which aggravated the situation. In this situation, communication and community empowerment approach is urgently needed, taking into account the circumstances and social change. This study aims to examine how socioeconomic conditions of communities around oil palm plantations and the extent of the role of community institutions in the activities of prevention and suppression of fires and haze, and determine the communication strategy of community empowerment in the prevention and suppression of fires and smoke haze oil palm plantations in South Kalimantan, Indonesia. the results showed that the alternative chosen strategy is as follows: (a) Improving socialization, campaigns and the effectiveness of inter-institutional communication for the prevention of forest fires; optimization of the institutional role of Fire Response Group at the grassroots level and improve the knowledge and skills of the community in the management of land resources in order to prevent fires.
Use of Information Communication Technologies Among Female Block Extension Ag...IOSRJAVS
Block Extension Agents are the extension workers who are specifically females and who carry out Gender sensitive issues for female farmers in Nigeria. They have the objective of seeing to the extension needs of the female farmers. The study assessed the use of Information Communication Technologies (ICTs) among Block Extension Agents (BEAs) in South-East Agro-Ecological Zone of Nigeria. Multi-stage sampling technique was used in the selection of the sample for the study. Data were collected with the aid of a structured questionnaire. The data was analysed using descriptive statistics. The mean age of the respondents was 40 years. All the BEAs sampled had formal education where more than half of them (51.04%) had first degree (B.Sc/B.Agric). The mean working experience of the respondents was 10 years. The mean income level of the respondents was N 33,875 per month. The extension agents claimed that twenty-five ICT facilities were available to them in varying degree. The attitude of the respondents towards the use of ICT facilities was positive (mean score=3.11). The extent of use of ICTs by the BEAs was low (mean score=1.45) which can be attributed to the low degree of availability of the ICTs than to their attitude towards the use of the ICT facilities. The BEAs identified thirteen (13) pressing problems that hindered their use of ICTs in varying degree and their major constraint (89.58%) as very limited access to the internet. It is recommended that the ADPs should provide such desirable working conditions that will encourage the BEAs to put in more years of their productive service to the ADPs; Workshops, conferences and seminars should be organized to train the BEAs in the use of ICTs which will improve efficiency in their work. ICT facilities should be made available to BEAs to enhance their productivity.
Study of Genotypic and Phenotypic Correlation among 20 Accessions of Nigerian...IOSRJAVS
Morphological techniques were used to evaluate the diversity in 20 cowpea accessions collected from some parts of Nigeria for two years (2007 and 2008) at Ibadan, South Western Nigeria. Correlation analysis was employed to show the relationships among the traits. Similarly, genotypic and phenotypic variances, genotypic coefficients of variation, heritability and expected genetic advance were estimated for the twelve traits in cowpea for each season. This study shows that for cowpea yield improvement, number of main branches, pod numbers, pods per plant, pods per peduncle and seeds per pod should be part of the selection criteria.
Analysis of Resource Use Efficiency in Small-Scale Maize Production in Tafawa...IOSRJAVS
This paper analyzed the resource-use efficiency of small-scale Maize production in Tafawa-Balewa local government area of Bauchi State. Data were collected from a sample of 120 Maize farmers selected through multi-stage sampling procedure using questionnaire and analyzed using simple descriptive statistics, double-log function and marginal value productivity analysis. The result showed that 90.17% had formal education; 51.67% were males; 90.17% were between the ages of 21-50. Majority 72.50% were married. In terms of farming experience, majority (86.67%) of the respondent had farming experience between 5-20 years. 75.00% had no contact with extension. The double-log function gave the best fit with Adjusted R2 of 81.16%. Production inputs such as seed, fertilizer, labour affected output significantly. Maize production in the study area has an increasing return to scale from the sum of elasticity of production (1.747). Seed and fertilizer were underutilized in Maize production, whereas labour was over used. The major problem confronting the farmers include high cost of inputs (77.50%); Untimely disbursement of credit/inputs (62.50; inadequate extension services (59.17); unstable price (41.67%); draught (33.33%), inadequate credit facilities (31.67%) etc. Profit could be enhanced by increasing the quantity used of seed and fertilizer inputs, its timely supply. Labour should be reduced to optimum level for increase output and total revenue respectively. It is also recommended that extension education and financial support to farmers be improved to allow them increase output and total revenue. There is need for adjustment in resource use in order to improve farm profit at this level of technology used by Maize farmers in the study area.
Role of Trace Minerals in Bolstering Reproductive Health in HeifersIOSRJAVS
Trace minerals play a significant role in improving reproductive health. A study was carried out to evaluate the role of trace minerals in alleviating reproductive health in heifers. A total of 20 heifers were allotted into three groups T0, T1 and T2. T0 (n=4) was kept as control. T1 (n=10) was supplemented with Uniselit (M/S Ayurvet) along with standard basal diet. T2 (n=6) was supplemented with CofeCu along with standard basal diet. Results revealed that there was significant improvement in the physical and chemical parameters of estrus exhibition in the Uniselit supplemented group T1 as compared to control. The conception rate was also higher in group T1 in comparison to control. Thus, it can be inferred that trace minerals have significant influence in improving reproductive health in heifers
Effect of Growth Retardants on Shoot and Root Development of Stevia(Steviareb...IOSRJAVS
Stevia rebaudianaBertoni is a natural sweetener herb, which is promising in food and pharmaceutical production. In Egypt, the gap between sugar production and consumption represents a serious problem, in order to close this gap; Stevia could be cultivated in the Egyptian environmental conditions. The objective of this study was to evaluate the effect of paclobutrazol(PBZ) and ethephon (ET) on shooting and rooting of stevia plant in vitroto improve the survival and growth of Stevia plantlets during acclimatization.The highest shoot number (48.0 shoots/explant) and the highest number of leaves (7.34) were recorded on MS medium containing 0.1mg/l PBZ. Whereas, the highest shoot length (7.36 cm) was obtained on MS medium without supplementation withPBZ or ET. The highest fresh and dry weights of shoots was recorded on MS medium containing 0.5 mg/l PBZ, which gave2.83 and 0.39 g, respectively. The highest number of roots (8.44 roots/shoot) was obtained with 0.1mg/l PBZ. Also, this study indicates that PBZ or ET increased chlorophyll a, chlorophyll b, carotenoids and carbohydrates content in the plant. PBZ and ET had significant effect on thesurvival of plants ex vitro, which recorded 85 and75%, respectively.
Effect of Different Levels of Fat on the Chemical Composition and Sensory Cha...IOSRJAVS
The effect of different levels of fat on the chemical composition and sensory characteristics has been made from the following levels of milk fat: 3%, 2.5% and 2%. The produced cheese was packed into sterile polyethylene bags (300 gram capacity) and stored at 4°C for three months. The chemical analysis was done at 0, 30, 60, 90 and 120 days. The results revealed that significant differences (P< 0.05) were found in protein, ash, total solid, pH, volatile fatty acids and fat contents of the different cheese samples. Sensory characteristics and storage periodof the cheese samples showed significant variations (P< 0.05). The protein, total solids and ash increased throughout the storage period, the lowest values were 21.82±3.49%, 59.29±4.03% and 5.60±0.89% respectively obtained at day zero of the storage. The highest pH occurred at day zero of storage. It was also found that the volatile fatty acids(VFA) increased with the decrease levels of fat, the highest VFA (2.62±0.50%01N ml NaOH/100 gm) was observed in the cheese with 2.5% fat. The statistical analysis showed sensory characteristics of the cheese samples significant (P< 0.05) affected by the fat levels. The present study concluded that production of low-fat cheeses with valuable chemical composition and good quality for the health benefits of the consumers.
Physicochemical and Sensory Characteristics of Sudanese LowFat Cheese during ...IOSRJAVS
This study was carried out in the milk processing units at College of Animal Production Science and Technology, Sudan University of Sciences during September 2014-January 2015. The chemical and sensory characteristics of Sudanese low-fat cheese were investigated as affected by different levels of gum Arabic and storage period. Three hundred and fifty liters (350) of standardized milk with (3%, 2.5% and 2%) of fat were prepared after purchase full cream milk from a private farm at Al Haj Yousif, Khartoum North. Seven treatments were carried out as follows: First treatment is the control in which cheese milk had no additive (3%) of fat. In the second and third with 3% of fat, fourth and fifth with 2.5% of fat and sixth and seventh with 2% of fat, 0.5 and 0.75% of gum Arabic were added respectively to cheese milk before pasteurization then low-fat cheese was made and stored at refrigerator (4°C) for 120 days. Physicochemical and sensory evaluation was done for the cheese samples at day zero, 30, 60, 90 and 120 days intervals. The cheese statistical analysis obtained that gum Arabic significantly (P< 0.05) affected the chemical composition of the cheese. The results also showed that crude protein fat, total solids, pH, volatile fatty acid and ash were significantly (P< 0.05) affected by both levels of gum Arabic and storage period. The results obtained there were significantly differences (P<0.05) between control cheese and the other with 0.5 and 0.75 gum Arabic respectively in all parameters
Assessmens of the Disease Index and the Behavior of Some Peach and Nectarine ...IOSRJAVS
Leaf curl of nectarine and peach, caused by Taphrina deformans (Berk.) Tul, is a difficult disease to control throughout Albania. Heavily infected fruit trees are poorly nourished, develop fewer flower buds, and are more easily damaged from freezing in winter. In this research, susceptibility of 2 peach and 2 nectarine varieties to leaf curl disease was investigated in ecological conditions of Tirane, Albania province in 2014 and 2016. Disease severity was calculated by the 0-5 scale when typical symptoms of leaf curl were evaluated on peach and nectarine leaves in naturally infected orchards. The conditions of natural infection allowed grouping the biological material in 4 classes (plant growth stages) and 6 groups of resistance for leaf curl disease severity. From the data we see that the of disease index (Imc-ja in%) during the three years of study varies from 4.22% in cultivar tire and reaches 22.8% in the Red Star cultivar we also comparisons this level too Control high infection which ranges from 40.66% in Roajal Black cultivar and up to 43.66% in Gomea cultivar.
Impact of Maize Silks Extract, its Application Methods and Their Interaction ...IOSRJAVS
The current study was conducted during the growing season (2014 – 2015), at a private orchard at Safwan area in Basra province-Iraq; to study the effect of several concentrations of maize silks, their application methods and their interaction on some indicators of growth and yield of tomato plants cv. Wijdan. The experiment was factorial and included (24) factorial treatments (4*2*3) resulted from: four levels of maize silks and two application methods, each in three replicates. The results showed a superiority of (60 ml. L.-1) of maize silks extract on most studied characteristics, which was non-significantly differed from the concentration (40 ml. L.-1). For the methods of application, the applying to soil method was superior with most characteristics. Concerning the interaction, the treatment (60 ml. L.-1 maize extract level + applying to soil method) was superior in its effect on most characteristics of tomato plant cv. Wijdan.
Nutritive Value of the Carcass of African Catfish (Clarias gariepinus Burchel...IOSRJAVS
The experiment on African catfish Clarias gariepinus fingerlings 3.55±0.01g average weight and 4.09±0.05cm average length, was to know the effect of feeding frequencies on the nutritive value on the carcass, the were fed with commercial feed (Coppens) of 58% crude protein level at 5% body weight, once (at 11:00 am), twice (9:00am and 4:00pm), thrice (9:00am, 1:00pm and 4:00pm), and four times (9:00am, 11:00am, 1:00pm and 4:00pm), daily to satiation for 14 weeks. The Mean Feed Consumption show that Treatment D had the highest total feed consumption of 54.10g, while the lowest feed consumption value of 43.20g was noted Treatment A which was the fish fed once per day. The mean proximate composition of the fish carcass show that crude protein was highest in Treatment D with 62.78±0.22, while Treatment A had the least with 54.72±0.02. Moisture content show that Treatment C had the highest with 11.86±0.14, while Treatment A had the least with 7.80±0.01. Ash content show that Treatment A had the highest with 6.90±0.22, while Treatment D had 1.08±0.63, which was the least. Crude lipid show that Treatment B had the highest with 11.78±0.17, while Treatment C had the least, with 9.24±0.33. The study suggests that body the composition of African catfish fingerlings is affected by the frequency of feeding.The results on feed utilization suggests that C. gariepinus fingerlings should be fed at four times per day for maximum growth and better survival
Hybrid optimization of pumped hydro system and solar- Engr. Abdul-Azeez.pdffxintegritypublishin
Advancements in technology unveil a myriad of electrical and electronic breakthroughs geared towards efficiently harnessing limited resources to meet human energy demands. The optimization of hybrid solar PV panels and pumped hydro energy supply systems plays a pivotal role in utilizing natural resources effectively. This initiative not only benefits humanity but also fosters environmental sustainability. The study investigated the design optimization of these hybrid systems, focusing on understanding solar radiation patterns, identifying geographical influences on solar radiation, formulating a mathematical model for system optimization, and determining the optimal configuration of PV panels and pumped hydro storage. Through a comparative analysis approach and eight weeks of data collection, the study addressed key research questions related to solar radiation patterns and optimal system design. The findings highlighted regions with heightened solar radiation levels, showcasing substantial potential for power generation and emphasizing the system's efficiency. Optimizing system design significantly boosted power generation, promoted renewable energy utilization, and enhanced energy storage capacity. The study underscored the benefits of optimizing hybrid solar PV panels and pumped hydro energy supply systems for sustainable energy usage. Optimizing the design of solar PV panels and pumped hydro energy supply systems as examined across diverse climatic conditions in a developing country, not only enhances power generation but also improves the integration of renewable energy sources and boosts energy storage capacities, particularly beneficial for less economically prosperous regions. Additionally, the study provides valuable insights for advancing energy research in economically viable areas. Recommendations included conducting site-specific assessments, utilizing advanced modeling tools, implementing regular maintenance protocols, and enhancing communication among system components.
Student information management system project report ii.pdfKamal Acharya
Our project explains about the student management. This project mainly explains the various actions related to student details. This project shows some ease in adding, editing and deleting the student details. It also provides a less time consuming process for viewing, adding, editing and deleting the marks of the students.
Democratizing Fuzzing at Scale by Abhishek Aryaabh.arya
Presented at NUS: Fuzzing and Software Security Summer School 2024
This keynote talks about the democratization of fuzzing at scale, highlighting the collaboration between open source communities, academia, and industry to advance the field of fuzzing. It delves into the history of fuzzing, the development of scalable fuzzing platforms, and the empowerment of community-driven research. The talk will further discuss recent advancements leveraging AI/ML and offer insights into the future evolution of the fuzzing landscape.
Industrial Training at Shahjalal Fertilizer Company Limited (SFCL)MdTanvirMahtab2
This presentation is about the working procedure of Shahjalal Fertilizer Company Limited (SFCL). A Govt. owned Company of Bangladesh Chemical Industries Corporation under Ministry of Industries.
Forklift Classes Overview by Intella PartsIntella Parts
Discover the different forklift classes and their specific applications. Learn how to choose the right forklift for your needs to ensure safety, efficiency, and compliance in your operations.
For more technical information, visit our website https://intellaparts.com
Final project report on grocery store management system..pdfKamal Acharya
In today’s fast-changing business environment, it’s extremely important to be able to respond to client needs in the most effective and timely manner. If your customers wish to see your business online and have instant access to your products or services.
Online Grocery Store is an e-commerce website, which retails various grocery products. This project allows viewing various products available enables registered users to purchase desired products instantly using Paytm, UPI payment processor (Instant Pay) and also can place order by using Cash on Delivery (Pay Later) option. This project provides an easy access to Administrators and Managers to view orders placed using Pay Later and Instant Pay options.
In order to develop an e-commerce website, a number of Technologies must be studied and understood. These include multi-tiered architecture, server and client-side scripting techniques, implementation technologies, programming language (such as PHP, HTML, CSS, JavaScript) and MySQL relational databases. This is a project with the objective to develop a basic website where a consumer is provided with a shopping cart website and also to know about the technologies used to develop such a website.
This document will discuss each of the underlying technologies to create and implement an e- commerce website.
Vaccine management system project report documentation..pdfKamal Acharya
The Division of Vaccine and Immunization is facing increasing difficulty monitoring vaccines and other commodities distribution once they have been distributed from the national stores. With the introduction of new vaccines, more challenges have been anticipated with this additions posing serious threat to the already over strained vaccine supply chain system in Kenya.
Cosmetic shop management system project report.pdfKamal Acharya
Buying new cosmetic products is difficult. It can even be scary for those who have sensitive skin and are prone to skin trouble. The information needed to alleviate this problem is on the back of each product, but it's thought to interpret those ingredient lists unless you have a background in chemistry.
Instead of buying and hoping for the best, we can use data science to help us predict which products may be good fits for us. It includes various function programs to do the above mentioned tasks.
Data file handling has been effectively used in the program.
The automated cosmetic shop management system should deal with the automation of general workflow and administration process of the shop. The main processes of the system focus on customer's request where the system is able to search the most appropriate products and deliver it to the customers. It should help the employees to quickly identify the list of cosmetic product that have reached the minimum quantity and also keep a track of expired date for each cosmetic product. It should help the employees to find the rack number in which the product is placed.It is also Faster and more efficient way.
Sachpazis:Terzaghi Bearing Capacity Estimation in simple terms with Calculati...Dr.Costas Sachpazis
Terzaghi's soil bearing capacity theory, developed by Karl Terzaghi, is a fundamental principle in geotechnical engineering used to determine the bearing capacity of shallow foundations. This theory provides a method to calculate the ultimate bearing capacity of soil, which is the maximum load per unit area that the soil can support without undergoing shear failure. The Calculation HTML Code included.
Automobile Management System Project Report.pdfKamal Acharya
The proposed project is developed to manage the automobile in the automobile dealer company. The main module in this project is login, automobile management, customer management, sales, complaints and reports. The first module is the login. The automobile showroom owner should login to the project for usage. The username and password are verified and if it is correct, next form opens. If the username and password are not correct, it shows the error message.
When a customer search for a automobile, if the automobile is available, they will be taken to a page that shows the details of the automobile including automobile name, automobile ID, quantity, price etc. “Automobile Management System” is useful for maintaining automobiles, customers effectively and hence helps for establishing good relation between customer and automobile organization. It contains various customized modules for effectively maintaining automobiles and stock information accurately and safely.
When the automobile is sold to the customer, stock will be reduced automatically. When a new purchase is made, stock will be increased automatically. While selecting automobiles for sale, the proposed software will automatically check for total number of available stock of that particular item, if the total stock of that particular item is less than 5, software will notify the user to purchase the particular item.
Also when the user tries to sale items which are not in stock, the system will prompt the user that the stock is not enough. Customers of this system can search for a automobile; can purchase a automobile easily by selecting fast. On the other hand the stock of automobiles can be maintained perfectly by the automobile shop manager overcoming the drawbacks of existing system.
Immunizing Image Classifiers Against Localized Adversary Attacksgerogepatton
This paper addresses the vulnerability of deep learning models, particularly convolutional neural networks
(CNN)s, to adversarial attacks and presents a proactive training technique designed to counter them. We
introduce a novel volumization algorithm, which transforms 2D images into 3D volumetric representations.
When combined with 3D convolution and deep curriculum learning optimization (CLO), itsignificantly improves
the immunity of models against localized universal attacks by up to 40%. We evaluate our proposed approach
using contemporary CNN architectures and the modified Canadian Institute for Advanced Research (CIFAR-10
and CIFAR-100) and ImageNet Large Scale Visual Recognition Challenge (ILSVRC12) datasets, showcasing
accuracy improvements over previous techniques. The results indicate that the combination of the volumetric
input and curriculum learning holds significant promise for mitigating adversarial attacks without necessitating
adversary training.
COLLEGE BUS MANAGEMENT SYSTEM PROJECT REPORT.pdfKamal Acharya
The College Bus Management system is completely developed by Visual Basic .NET Version. The application is connect with most secured database language MS SQL Server. The application is develop by using best combination of front-end and back-end languages. The application is totally design like flat user interface. This flat user interface is more attractive user interface in 2017. The application is gives more important to the system functionality. The application is to manage the student’s details, driver’s details, bus details, bus route details, bus fees details and more. The application has only one unit for admin. The admin can manage the entire application. The admin can login into the application by using username and password of the admin. The application is develop for big and small colleges. It is more user friendly for non-computer person. Even they can easily learn how to manage the application within hours. The application is more secure by the admin. The system will give an effective output for the VB.Net and SQL Server given as input to the system. The compiled java program given as input to the system, after scanning the program will generate different reports. The application generates the report for users. The admin can view and download the report of the data. The application deliver the excel format reports. Because, excel formatted reports is very easy to understand the income and expense of the college bus. This application is mainly develop for windows operating system users. In 2017, 73% of people enterprises are using windows operating system. So the application will easily install for all the windows operating system users. The application-developed size is very low. The application consumes very low space in disk. Therefore, the user can allocate very minimum local disk space for this application.
Water scarcity is the lack of fresh water resources to meet the standard water demand. There are two type of water scarcity. One is physical. The other is economic water scarcity.
Characterization and Geographical Classification of Greek Fir Honeys Based on Physicochemical Parameters, Colour Attributes, and Volatile Compounds Using Chemometrics
1. IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS)
e-ISSN: 2319-2380, p-ISSN: 2319-2372. Volume 10, Issue 1 Ver. I (January. 2017), PP 16-22
www.iosrjournals.org
DOI: 10.9790/2380-1001011622 www.iosrjournals.org 16 | Page
Characterization and Geographical Classification of Greek Fir
Honeys Based on Physicochemical Parameters, Colour
Attributes, and Volatile Compounds Using Chemometrics
Ioannis K. Karabagiasa*
, Evangelia Z. Halatsia
, Stavros Kontakosb
,
Michael G. Kontominasa
a
Laboratory of Food Chemistry Department of Chemistry University of Ioannina, Ioannina 45110, Greece
b
Democritus University of Thrace, Department of Social Administration and Political Science, Komotini-
69100, Greece
Abstract: The objective of the present study was to characterize commercial fir honey produced in different
regions in Greece and investigate the possibility of geographical classification using data sets of
physicochemical parameters, colour attributes, and volatile compounds using multivariate analysis techniques.
For this purpose, 43 fir honey samples were collected during harvesting periods 2010-2013 from five different
regions in Greece. Physicochemical parameters (electrical conductivity, ash, pH, moisture, free acidity, lactonic
acidity, total acidity, lactonic to free acidity ratio) were determined using official methods of analysis, while
colour attributes (L*, a*, b*) and browning index were determined using CIE recommendations and
methodology published in the literature, respectively. Finally, volatile profiles were determined using headspace
solid phase microextraction coupled to gas chromatography/mass spectrometry. Results showed that honey
samples analyzed met the criteria set by regulatory standards for fir honey, and exhibited variations in
physicochemical parameter values, colour attributes, and volatile profiles, according to geographical origin.
On the basis of the results obtained, it is possible to classify (correct prediction 69.8%) commercial fir honeys
produced in specific regions, using a data set of 21 parameters and chemometrics.
Keywords: characterization, commercial honey, physicochemical parameters, colour, browning index,
volatiles, chemometrics
I. Introduction
Apis mellifera honeybees produce honey, a viscous and highly concentrated solution of a complex
mixture of sugars, through the collection of nectar from different flowers or honeydew secretions, and the action
of enzymes (mainly invertase and glucose oxidase) produced in their hypopharyngeal glands. Honey contains
also water and small amounts of other nutrients such as minerals, proteins, vitamins, organic acids, flavonoids,
phenolic acids, and other phytochemicals. Phytochemicals have been characterized as health promoting agents
and are responsible for the antioxidant activity of honey [1].Honey composition, colour, flavour, as well as the
content of phytochemicals depend on the flower sources, geographical origin, climatic conditions, bee species,
packaging conditions, thermal and ultrasound treatments, storage time, etc. [1,2]. Variation in honey
composition and properties have attracted researchers in conducting a great number of studies, dealing with
characterization and authentication [1-5], using instrumental and conventional methods in combination with
statistical analyses.
In Greece, previous studies have dealt with the characterization and geographical classification of pine
and thyme honeys based on volatile compounds [3] or the combination of physicochemical parameters
(including colour) and volatile compounds [2] using chemometrics. Yet, there is no other study involving fir
honey geographical classification, using physicochemical parameter, colour attribute, and volatile compound
data. Based on the above, the aim of the present study was to investigate if specific physicochemical parameters,
including colour, and volatile compounds, could allow the differentiation of fir honey according to geographical
origin, using chemometrics.
II. Experimental
2.1. Honey samples
A total of 43 commercial fir honeys samples were donated by ATTIKI Honey S.A. and local beekeepers,
during the harvesting periods 2010-2013, originating from five different regions in Greece: Messinia (7
samples: Abies cephalonica and fir honeydew), Lakonia (11 samples: Abies cephalonica and fir honeydew),
Arkadia (9 samples: Abies cephalonica and fir honeydew), Karditsa [12 samples: Abies cephalonica)], and
Karpenisi [4 samples: Abies cephalonica)], where fir honey is traditionally produced. The botanical origin of
2. Characterization and Geographical Classification of Greek Fir Honeys Based on Physicochemical
DOI: 10.9790/2380-1001011622 www.iosrjournals.org 17 | Page
honey samples was determined using melissopalynological analysis [2]. Samples were stored in glass
containers, shipped to the laboratory and maintained at 4±1°
C until analysis.
2.2. Reagents, solutions, and consumables
Sodium hydroxide and hydrochloric acid (37%) used for the determination of free and lactonic acidity
were purchased from Sigma Aldrich (Germany). Potassium chloride (0.1M) was obtained from Hanna (HI
7031, Hanna Instruments, Inc., Woonsocket, USA). Whatman filters (CAT. No. 6780-2504, UK) with a pore
size of 0.45 μm, were used to remove any solid particles prior to spectrophotometric measurements.
2.3. Physicochemical parameters
Physicochemical parameters such as electrical conductivity (EC), pH, moisture, free acidity (FA), lactonic
acidity (LA), total acidity (TA), as well as the ratio of lactonic to free acidity (LA/FA) were determined
according to harmonized methods of international honey commission [6]. The conductivity meter (Delta OHM,
model HD 3456.2, Padova, Italy) was calibrated with a 1413 μS/cm standard solution of 0.1M potassium
chloride at 20 °
C. Conductivity results were expressed as mS/cm. Each sample was run in triplicate (n=3).
2.4. Determination of ash content
The ash%, (w/w) was calculated on the basis of the results of electrical conductivity [7].
2.5. Determination of colour attributes: (L*, a*, b*), and browning index
Colour parameters (L*, a*, b*) were determined according to CIE recommendations [13], as shown in previous
study [10]. Browning index (BI), BI= [100(x-0.31)/0.172], where x= (a*+1.75L*)/(5.645L* +a*-3.012b*) was
estimated according to Ferrari et al.[8]. The results reported are the mean of five determinations (n=5).
2.6. HS-SPME-GC/MS analysis
Headspace volatile compounds were extracted from honey, using a divinyl benzene/carboxen
/polydimethylsiloxane (DVB/CAR/PDMS) fiber 50/30μm (Supelco, Bellefonte, PA, USA). Prior to use, the
fiber was conditioned following the manufacturer’s recommendations. Test samples were prepared daily prior to
HS-SPME-GC/MS analysis. Blank runs were carried out before sample analysis to make sure that there was no
contamination that could cause memory effects. Details on identification, semi-quantification, along with the
GC/MS instrumentation and analysis conditions are given in a previous study [2]. Each sample was run in
duplicate (n=2).
2.7. Statistical Analysis
In order to test differences between the determined parameters with respect to geographical origin,
multivariate analysis of variance (MANOVA) and linear discriminant analysis (LDA) were applied, as
previously described [2], to the collected data set. All the statistical treatment of data was performed using the
SPSS v.22.0 statistics software.
III. Results and discussion
3.1. Physicochemical parameters of commercial fir honeys
Water represents the second major constituent of honey. Water content or moisture (g/100g) is related
to different factors such as botanical and geographical origin of the nectar, soil and climatic conditions (rainfall,
relative humidity), season of harvesting, degree of maturation, beekeeper’s manipulation during period of
harvesting, as well as extraction, processing and storage conditions [9]. Honeys from different geographical
origin, may have different moisture content, as in the present study. Furthermore, moisture is a quality
parameter, related to honey shelf life. In the European regulation [10], the upper limit for moisture content in
honey is 20g/100g. Higher moisture content may result to an undesirable fermentation, due to the osmophilic
(sugar tolerant) yeasts and moulds present in honey. On the other hand, water content may affect several honey
properties such as colour, crystallization, viscosity, flavour, and density [11]. The moisture content in honeys
analyzed was below 20g/100g, in agreement with Turkish, Spanish, and Slovak honeydew honeys, respectively
[12-14]
pH value of honey usually ranges between 3.20 and 4.50 in nectar or blossom honeys and 5.0 to 6.0, in
honeydew honeys. Present pH values were typical of honeydew honeys, being in the range reported previously
for honeys originating from Turkey, Spain, and Greece [2, 12, 13].
Electrical conductivity of honey is owed to its ions, organic acids and proteins and is related to the ash
content [7]. Thus, the higher the ash content, the higher the resulting conductivity. It is often used as the
dominant criterion in the quality control of honey, in order to distinguish between blossom and honeydew
honeys. EC values in honeydew honeys should be higher or equal to 0.80 mS/cm [10]. All honeys analyzed met
this criterion, in agreement with previous studies on honeydew honeys [2, 12, 13]. Ash content (g/100g) is a
measure of quality that evaluates the mineral content present in honey. The mineral content may comprise an
indicator of geographical origin or environmental pollution, since mineral content depends on the type of soil
used for the growth of plants, having the nectar which honeybees feed on [15].Acidity (meq/kg) is an important
parameter related to the freshness and the deterioration of honey. It is characterized by the presence of organic
acids in equilibrium with lactones, esters and some inorganic ions (phosphates, sulfates and chlorides) resulting
3. Characterization and Geographical Classification of Greek Fir Honeys Based on Physicochemical
DOI: 10.9790/2380-1001011622 www.iosrjournals.org 18 | Page
to free and lactonic acidity (reverse acidity when honey becomes alkaline). The maximum value for free acidity
set by the EU [10] is 50 meq/kg. Higher values may be indicative of undesirable fermentation of sugars into
organic acids. However, the presence of different organic acids, geographical origin and harvest season may
also affect honey’s acidity [15]. FA values were much lower than the upper limit set by the EU [10]. Total
acidity (sum of free and lactonic acidity) recorded variations according to the geographical origin of commercial
fir honeys (Table 1), but it was lower than 50 meq/kg. Lactonic to free acidity ratio, varied significantly
(p=0.001) according to geographical origin of fir honeys. Variations in acidity related parameters have been
also previously reported for Turkish, Spanish, Slovak, and Greek honeydew honeys [2, 12, 13, 14].
3.2. Colour attributes and browning index of commercial fir honeys
Commission Internationale de l' Eclairage (CIE) system uses three parameters to evaluate colour in
foodstuffs: colour parameter L* corresponds to degree of brightness, parameter a* (positive values) corresponds
to degree of redness, a* (negative values) to degree of greenness, parameter b* corresponds to yellowness of
colour (when positive) and to blueness of colour (when negative) [16]. This system stimulates the human eye
observation when irritated with a specific colour. In that sense, honey colour is a very important attribute for
consumer product acceptance. The colour of honey may provide useful information, involving: the content of
pigments with antioxidant properties (carotenoids, phenolic acids, flavonoids, anthocyanins, etc.), nectar source
and pollen content, or mineral content [17]. In addition, processing of honey (i.e. thermal treatment,
ultrasonication) may affect honey colour [1].
Results showed that significant differences (p=0.0024) were observed only for colour parameter a*
according to geographical origin of commercial fir honeys (Table 1). L* values were higher as compared to the
work of Escriche et al. [4] dealing with honeys from Spain, Romania, and Czech Republic (acacia, sunflower,
tilia), whereas they were in agreement with previous work on Greek pine ( honeydew) honeys [10]. However,
a* and b* colour parameters were much lower as compared to commercial honeys from Thailand and Mexico,
respectively [1, 18]. The browning index (BI) demonstrates the effect of thermal treatments on the development
of dark colour in honey samples. The browning reaction could occur as a consequence of increased temperature
during honey processing. In the work of Tornuk et al. [15] it was shown that the brown pigment development in
honey was correlated to the thermal processing of honey particularly owed to the non-enzymatic browning (
Maillard reaction). On the other hand, the Maillard reaction depends to a great extend, on the presence of high
concentration of sugar and amino acids interacting at elevated temperatures. Besides, caramelization of sugars
or presence of heat sensitive compounds during thermal processing may increase BI in honey [17]. Present
results showed that commercial fir honeys were not thermally treated, since browning index values were much
lower as compared to thermally or ultrasound treated commercial honeys [1]. This comprises an additionally
quality criterion for fir honey consumers.
Table 1. Physicochemical parameter, colour, and browning index values of commercial fir honeys according to
geographical origin
N: number of honey samples. Mean: average value of three (n=3) and five (n=5) determinations for physicochemical and colour parameters,
respectively. SD: standard deviation. MANOVA in comparison of means (p<0.05).
3.3. Volatile compounds of commercial fir honeys
Forty two volatile compounds (including furfural) were identified and semi-quantified using internal
standard (benzophenone) [2]. These compounds belong to different classes: acids, esters, alcohols, aldehydes,
ketones, terpenoids, norisoprenoids, hydrocarbons, benzene derivatives, furan derivatives, etc. Table 2 lists the
4. Characterization and Geographical Classification of Greek Fir Honeys Based on Physicochemical
DOI: 10.9790/2380-1001011622 www.iosrjournals.org 19 | Page
15 key volatile compounds which served as markers of geographical origin. Full volatile data is given as
supplementary files.
A typical chromatogram of fir honey (no.1) from Karpenisi, pointing out the key volatile markers of
geographical origin, numbered according to Table 2, is given in Fig.1. Numerous of these compounds including:
2-ethyl-1-hexanol, 1-octanol, 1-nonanol, furfural, benzaldehyde, octanal, decanal, benzeneacetaldehyde,
heptane, 1-octene, octane, nonane, 2,5-dimethyl-furan, borneol, 3,4,5-trimethyl-phenol, have been identified in
Greek and Turkish pine honeys [19, 20] as well as in fir honeys from Croatia [21]. What is remarkable is that,
the ethyl esters identified in the present study, have not been reported previously in monofloral honeys produced
in different countries [3, 20, 21, 22]. These compounds are reported for the first time in fir honey, and may
originate from resins secreted in Greek fir trees. It should be stressed that, honey aroma, along with colour and
taste, are among of the most important parameters affecting product acceptability by consumers. Furthermore,
numerous volatile markers have been used for the determination of geographical and botanical origin of
particular honey samples [2, 3, 4, 20], using chemometrics.
Table 2. Key volatile compounds (mean values, mg/kg) of commercial fir honeys of different geographical
origin
RT: retention time (min), nd: not detected, every value is the average of two replicates (n=2), a
KI: Experimental Kovats index
values using hydrocarbons being naturally present in honey, b
Method of identification: MS, identification by comparison with MS data in
Wiley 7 NIST 2005 mass spectral library/ KI, identification by comparison of Kovats index with the literature cited or included in the Wiley
library. Class of compounds is presented alphabetically. nd: not detected. GOM: geographical origin marker as defined by comparison of
mean values using MANOVA and LDA.
5. Characterization and Geographical Classification of Greek Fir Honeys Based on Physicochemical
DOI: 10.9790/2380-1001011622 www.iosrjournals.org 20 | Page
Figure 1. A typical chromatogram of commercial fir honey (no.1) from Karpenisi. Volatile markers are
numbered according to Table 3.IS: internal standard.
3.4. Classification of fir honey according to geographical origin based on physicochemical parameters,
colour attributes, and volatile compounds
The 43 fir honey samples were subjected to MANOVA analysis to determine which physicochemical
parameters, colour attributes, and volatile compounds are significant for the geographical classification.
Dependent variables included the 11 physicochemical parameters (including colour) and the 41 volatile
compounds (with the exception of furfural) while geographical origin was taken as the independent variable.
Pillai’s Trace=1.008 (F=65.581, p=0.001) and Wilks’ Lambda=0.001 (F=71.428, p=0.002) index values
showed the existence of a significant multivariable effect of honey physicochemical parameters, colour
attributes, and volatile compounds on geographical origin of fir honey. Six physicochemical parameters
(including colour) and 15 volatile compounds were found to be significant (p<0.05) for the geographical
classification of commercial fir honeys. Thus, these 21 parameters were subjected to LDA.
Results showed that two statistically significant discriminant functions are formed. (Wilks’ Lambda=0.001,
X2
=209.306, df=80, p=0.001) for the first function and (Wilks’ Lambda=0.032, X2
=101.235, df=57, p=0.001)
for the second. A significant value of Wilks’ Lambda index shows that the discriminant function is basic for the
differentiation of the investigated groups. Testing of the uniformity of variability (Box’s M= 456.712, F=2.321,
p=0.051) was insignificant at the 95% confidence level showing the existence of uniformity of sample
variability for each geographical origin.
The first discriminant function accounted for 82.6% of total variance and the second accounted for
10.4%. Both accounted for 93 % of total variance. In Fig. 2 it is shown that all the geographical regions are well
separated with Karditsa and Karpenisi being relatively close to one another. The first discriminant function
clearly differentiates Karditsa and Karpenisi from all the other regions, while the second discriminant function
clearly differentiates Messinia and Lakonia. The dependent variables that contributed to the first and second
discriminant function, resulting to a correct geographical classification of fir honeys, are shown in Table 3.
The overall correct classification rate was 100% using the original and 69.8% the cross validation method; the
latter considered a satisfactory value especially for the second ‘’conservative’’ method [2].
Figure 2. Geographical classification of commercial fir honeys using 21 parameters
6. Characterization and Geographical Classification of Greek Fir Honeys Based on Physicochemical
DOI: 10.9790/2380-1001011622 www.iosrjournals.org 21 | Page
Table 3. Physicochemical parameters, colour attributes, and volatile compounds served as markers of fir honey
geographical origin
Pooled within-groups correlations between discriminating variables and standardized canonical discriminant functions Variables
ordered by absolute size of correlation within function. 1
Largest absolute correlation between each variable and any discriminant function.
Present classification results, are lower as compared to those reported previously for Greek pine
honeys (based on the use of physicochemical parameters and volatile compounds), and thyme honeys (based on
the use of volatile compounds), respectively [2, 3]. It should also be mentioned that such data involving fir
honey, has not been previously published in literature regarding Greek fir honey.
Along the same line of reasoning, Juan–Borrás et al. [4], reported a higher classification rate than that of the
present work in a study involving acacia, tilia, and sunflower honeys from Spain, Romania, and Czech republic,
respectively, using physicochemical parameters in combination with volatile compounds and chemometrics. At
this point it should be noticed that, in the previous work of Karabagias et al. [2] and Alissandrakis et al. [3] the
geographical regions investigated were 4, and were of no close proximity as in the present study. The same
holds for the work of Juan–Borrás et al. [4], which involved different honey types produced in different
countries. We claim that it is more difficult to differentiate honeys from the same production country or from
areas of close proximity.
IV. Conclusions
Results of the present study showed that specific physicochemical and colour parameters, along with
volatile compounds and chemometrics, may provide useful information regarding commercial fir honey
geographical origin. This is the first report on such a topic, this constituting the novelty of the present work.
Differences in the classification rates may be also attributed to the presence of different flowers in the proximity
of the investigated regions. However, this is expected since honeybees collect nectar or honeydew from a large
number of plants or trees, grown in a given area. Finally, in honey authentication efforts it should be considered
that, if the tested regions or physicochemical parameters investigated increase, then correct classification rates
are expected to decrease. This fact may justify the relatively low correct prediction rate recorded in the present
study.
Acknowledgments
The authors would like to thank ATTIKI Honey S.A. for the donation of fir honey samples from
Karditsa and Karpenisi. Technical assistance of Dr. Sofia Karabournioti in the melissopalynological analysis is
greatly acknowledged. The assistance of local beekeepers from Messinia, Lakonia, and Arkadia in the
collection of fir honey samples is also acknowledged.
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