This document presents the design of a low voltage differential CMOS transconductance amplifier operating in the sub-threshold region of 0.5V to 1.5V. A 180nm CMOS technology is used in the design on Cadence. Simulation results show the amplifier achieves a maximum differential output at a bias current of 500nA, with a common mode rejection ratio of 88dB and static power consumption of 241nW under normal input conditions. The layout is presented and verified using DRC and LVS tools in Cadence.