2. Objectives
To know difference between immunity and hypersensitivity
To know different types of hypersensitivity
To know mechanisms involved in hypersensitivity
Mohammad Mukhit Kazi Lecturer SDCH Pune
3. Introduction
Immunity
A protective process
Helps body to overcome infections
Specific response to antigens
Immune response – sometime injurious to host
A damage to the host, mediated by preexisting
immunity to self or foreign antigen.
Mohammad Mukhit Kazi Lecturer SDCH Pune
4. Definition of hypersensitivity
The injurious consequences in the sensitized host, following
contact with the specific antigen.
For induction of hypersensitivity reactions the host should
have had contact with the antigen (allergen)
Initial contact sensitizes the immune system, leading to the
priming of the appropriate B or T lymphocytes this is known
as sensitizing or priming dose
Subsequent contact with the allergen causes manifestations of
hypersensitivity as shocking dose.
Mohammad Mukhit Kazi Lecturer SDCH Pune
5. Difference between Immunity and
Hypersensitivity
Focus is on antigen
and what happens to
it
Antigen are of little
concern or often
innocuous or bland
substances such as serum
proteins or pollen
e.g. killing of bacterium
or neutralization of toxin
It is concerned with what happens
to the host as a result of the
immune reaction
Mohammad Mukhit Kazi Lecturer SDCH Pune
6. Musts for Hypersensitivity
Contact with allergen
Sensitizing/priming dose
Induction of AMI/CMI
Shocking dose
Mohammad Mukhit Kazi Lecturer SDCH Pune
7. Allergy
Originally used by von Pirquet means
An altered state of reactivity to an antigen and included both
types of immune responses protective as well as injurious
Also referred as all immune processes harmful to the host such
as hypersensitivity and autoimmunity
Commonly used as hypersensitivity
Mohammad Mukhit Kazi Lecturer SDCH Pune
8. Classification of Hypersensitivity
reactions
I. Immediate hypersensitivity (B cell or Ab mediated)
Anaphylaxis
Atopy
Antibody mediated cell damage
Arthus phenomenon
Serum sickenss
II. Delayed hypersensitivity (T cell mediated)
Infection (tuberculin) type
contact dermatitis type
Mohammad Mukhit Kazi Lecturer SDCH Pune
9. Immediate hypersensitivity Delayed Hypersensitivity
1. Appears and recedes rapidly 1. Appears slowly, lasts longer
2. Induced by antigen or haptens by
any route
2. Antigen or hapten intradermally
or with Freund’s adjuvant or by
skin contact
3. Circulating antibodies present
and responsible for reaction; (Ab
mediated reaction)
3. Circulating Abs may be absent
and not responsible for reaction;
cell mediated reaction
4. Passive transfer possible with
serum
4. Cannot be transferred with
serum; but possible with T cells or
transfer factor
5. Desensitization easy but short
lived
5. Difficult, but long lasting
Mohammad Mukhit Kazi Lecturer SDCH Pune
10. Coombs and Gell (1963) classified hypersensitivity
reactions into 4 types based on different
mechanisms of pathogenesis
Type I (Anaphylactic, IgE or reagin dependent)
Type II ( cytotoxic or cell stimulating)
Type III ( Immune complexes or toxic complex disease)
Type IV ( Delayed or cell mediated hypersensitivity)
Mohammad Mukhit Kazi Lecturer SDCH Pune
13. A = Type I - Anaphylaxis Reaction
C = Type II - Cytotoxic Mediated Hypersensitivity Reaction
I = Type III - Immune Complex Mediated Hypersensitivity
Reaction
D = Type IV - Delayed Hypersensitivity Or Cell Mediated
Hypersensitivity Reaction
Mohammad Mukhit Kazi Lecturer SDCH Pune
14. Type I Reactions (IgE dependent)
Occurs in two forms
The acute : potentially fatal, systemic form called anaphylaxis
The chronic or recurrent : Non fatal, typically localized form
called atopy
Mohammad Mukhit Kazi Lecturer SDCH Pune
16. Anaphylaxis
Classical immediate hypersensitivity reaction
Ana = without
Phylaxis = protection
Coined by Richet (1902)
Experiment on Dog
Theobald’s smith (1902)
Similar phenomenon in guinea pig
Ehrlich named this ‘Theobald Smith phenomenon’
Mohammad Mukhit Kazi Lecturer SDCH Pune
17. Type I Reactions
Humans –
Itching of scalp & tongue, flushing
of skin, difficulty in breathing,
nausea, vomiting, diarrhea, acute
hypotension, loss of consciousness,
death (rare)
Causes
Serum therapy, antibiotics, insect
stings
Treatment
Adrenalin 0.5 ml (1 in 1000
solution) SC/IM repeated up to
2 ml in 15 min
Mohammad Mukhit Kazi Lecturer SDCH Pune
18. Sensitization is most effective given parenterally
But may occur by any route
Minute doses can sensitize the host
Antigens and haptens can induce anaphylaxis
There should be interval of 2-4 weeks between sensitizing dose
and shocking dose
Shocking dose more effective when given IV
It should be identical to sensitizing dose
Mohammad Mukhit Kazi Lecturer SDCH Pune
19. The clinical effects are due to smooth muscle contraction and
increased vascular permeability
There is also edema, decreased coagulability of blood, fall in BP
and temp. leucopenia and thrombocytopenia
Guinea pigs are more susceptible and rats are resistant
Rabbits, dogs and humans are intermediate susceptible
Mohammad Mukhit Kazi Lecturer SDCH Pune
20. In human beings fatal anaphylaxis fortunately rare
Signs and symptoms begin with itching of the scalp and tongue,
flushing of the skin over whole body and difficult in breathing
due to bronchial spasm
There may be nausea, vomiting, abdominal pain and diarrhea,
sometime blood in stool
Acute hypotension, loss of consciousness & death follows
Mohammad Mukhit Kazi Lecturer SDCH Pune
21. Insect stings cause anaphylaxis in human beings
Prompt treatment with adrenalin is life saving
0.5 ml of a 1 in 1000 solution SC or IM
Repeat dose to total 1 ml over 15 minutes if necessary
Cutaneous anaphylaxis
Local wheal and flare response after Ag is administered intradermally
Wheal – pale central area
Flare – hyperemia and erythema
Mohammad Mukhit Kazi Lecturer SDCH Pune
22. Passive cutaneous anaphylaxis (PCA)
Developed by Ovary (1952)
For detection of Abs e.g. IgG Ab
Anaphylaxis in vitro
Schultz – Dale phenomenon : Isolated tissues from sensitized guinea pig held in
Ringers solutions will contract vigorously
Mohammad Mukhit Kazi Lecturer SDCH Pune
23. Mediators for Anaphylaxis
Primary mediators
Histamine : imp vasoactive amine formed by decarboxylation of histidine found
in mast cells, basophils and platelets
Released into skin stimulates nerves producing burning and itching sensations
Causes vasodilatation and hyperemia, edema by increasing capillary permiability
Mohammad Mukhit Kazi Lecturer SDCH Pune
24. Induces smooth muscle contraction and stimulates
Serotonin
Decarboxylation of tryptophan found in intestinal mucosa, brain tissue and platelts
Causes smooth muscle contraction
Increased capillary permeability and vasoconstriction
Chemotactic factors
Eosinophil chemitactic factors are acidic tetrapeptides released from mast cell granules
Strongly chemotactic
Mohammad Mukhit Kazi Lecturer SDCH Pune
25. Secondary mediators
Prostaglandins and leukotrienes
Formed from disrupted cell membrane of mast cells & other leucocytes
They are powerful bronchoconstrictors
Prostaglandins also affect secretion by mucous glands, platelet adhesion, permeability & dilatation
of capillaries and the pain threshold
Platelet activating factor
Low molecular weight lipid causes aggregation of platelets and release vasoactive amines
Other mediators of anaphylaxis
Anaphylatoxin released by complement activation and bradykinin
Mohammad Mukhit Kazi Lecturer SDCH Pune
26. Anaphylactoid reaction
Intravenous injection of peptone, trypsin provokes a clinical reaction
resembling anaphylactic shock termed as anaphylactoid reaction
Resemblance is due to same chemicals participating in the reaction
It has no immunologic basis
Non specific mechanism
Mohammad Mukhit Kazi Lecturer SDCH Pune
27. Atopy
Introduced by Coca (1923)
Naturally occurring : hay fever and asthma
Antigens are inhalant or ingestants
Difficult to induce artificially
Genetically determined probably linked to MHC genotypes
Produce large quantity of IgE antibodies
Bottle fed infants tend to develop atopy in later life
IgE differs from other immunoglobulin in many ways
Clinical expression depends portal of entry
Conjunctivitis, rhinitis, gastrointestinal symptoms, dermatitis
Mohammad Mukhit Kazi Lecturer SDCH Pune
28. 1. Cannot be demonstrated in conventional serological reactions
2. Commonly occur in humans not easy in experimental animals
3. IgE is homocytotropic ie species specific
4. Heat sensitive and is inactivated at 56o
c in 2 – 4 hours.
5. Does not pass through placenta
Mohammad Mukhit Kazi Lecturer SDCH Pune
29. Type II Reaction: Cytolytic and Cytotoxic
Involve a combination of IgG antibodies with antigenic
determinant on the surface of cells
Leading o cytotoxic or cytolytic effects
Lysis of RBCs by antiRBCS antibodies
Mohammad Mukhit Kazi Lecturer SDCH Pune
30. Type III Reactions: Immune complexes Diseases
Arthus reaction
Arthus in 1903 observed after repeated injection of normal horse
serum in rabbits subcutaneously develops edema, indurations and
hemorrhagic necrosis
Tissue damage is due to antigen – antibody precipitates causing
complement activation and release of inflammatory molecules
This leads to increased vascular permeability and infiltration of the
site with neutrophils
Leukocyte platelet thrombi are formed that reduces the blood supply
and lead to tissue necrosis
Mohammad Mukhit Kazi Lecturer SDCH Pune
32. • Serum Sickness
systemic form of type III hypersensitivity
Described by von Pirquet 1905
Appeared 7 – 12 days following a single injection of high concentration of
foreign serum such as diphtheria antitoxin
Clinical syndrome consists of
Fever
Lymphadenopathy
Splenomegaly
Arthritis
Glomerulonephritis
Endocarditis
Vasculitis
Urticarial rashes
Abdominal pain
Nausea
Vomiting
Pathogenesis is the formation immune complex get depositied on inner
lining of blood vessels in various parts of body causing inflammatory
infiltration
Mohammad Mukhit Kazi Lecturer SDCH Pune
34. Depletion of complement
Disease is self limited
Single injection can serve both as the sensitizing dose and the
shocking dose
Immune complexes occurs in many diseases including bacterial,
viral and parasitic infections, disseminated malignancies and
autoimmune conditions
Mohammad Mukhit Kazi Lecturer SDCH Pune
35. Type IV Reactions: Delayed hypersensitivity
Provoked by intra cellular parasites or haptens
Reaction due to sensitized T cells which on contact with specific
antigen releases cytokines that causes biological effects
Cannot be transferred passively
But can be transferred by lymphocytes
Two types
Tuberculin
Contact dermatitis type
Mohammad Mukhit Kazi Lecturer SDCH Pune
36. Tuberculin type
When small dose of tuberculin is injected intradermally in an
individual sensitized to tuberculoprotein by prior infection or
immunization an indurated inflammatory reaction develops
at the site within 48 – 72 hours
In unsensitized individuals no response
Useful indication of the state of DH to the bacilli
Mohammad Mukhit Kazi Lecturer SDCH Pune
37. Contact dermatitis type
Due to variety of chemicals, metals, dyes
Contact dermatitis is an inflammation of the skin caused
by direct contact with an irritating or allergy-causing
substance (irritant or allergen). Reactions may vary in the
same person over time. A history of any type of allergies
increases the risk for this condition.
Mohammad Mukhit Kazi Lecturer SDCH Pune
40. Type-IVType-IVType-IIIType-IIIType-IIType-IIType-IType-Icharacteristiccharacteristic
Comparison of hypersensitivity
reactions
Comparison of hypersensitivity
reactions
TB test, poisonTB test, poison
ivy, granulomaivy, granuloma
farmers’farmers’
lung, SLElung, SLE
pemphigus,pemphigus,
GoodpastureGoodpasture
hay fever,hay fever,
asthmaasthma
examplesexamples
antibody IgE IgG, IgM IgG, IgM none
antigen exogenous cell surface intracellularsoluble
response
time
15-30 min. Min.-hrs 3-8 hours 48-72 hours
or longer
appearance Wheal &
flare
Lysis &
necrosis
Erythema &
edema
Erythema &
induration
baso- and
eosinophils
Ab and
complement
histology PMN and
complement
Monocytes &
lymphocytes
T-cellsantibodyantibodyantibodytransfer with
Mohammad Mukhit Kazi Lecturer SDCH
Pune
41. Autoimmunity
Self antigens are not immunogenic
Autoimmunity is a condition in which structural or
functional damage is produced by the action of
immunologically competent cells or antibodies against the
normal components of the body.
Autoimmunity literally means – protection
Mohammad Mukhit Kazi Lecturer SDCH Pune
42. Mechanism of autoimmune
diseases
Cells undergo antigenic alteration due to physical, chemical
or biological influences
This results in immune response
Physical agents – irradiation
Chemicals – drugs
Biological – infectious agents such as viruses
Mohammad Mukhit Kazi Lecturer SDCH Pune
43. Classification of Autoimmune
diseases
Hemolytic autoimmune disease
- Autoimmune hemolytic anemias
- Autoimmune thrombocytopenia
- Autoimmune leucopenia
Mohammad Mukhit Kazi Lecturer SDCH Pune
44. Localized autoimmune diseases
- Hashimoto’ disease (enlargement of thyroids)
- Grave’s disease (Abs to thyroglobulin)
- Addision’s disease (lymphocyte infiltration in adrenal glands)
- Autoimmune orchitis
- Myasthenia gravis (malfunction of myoneural junction)
- Autoimmune disease of eye
- Pernicius anemia
- Autoimmune disease of nervous system
- Autoimmune disease of skin
Mohammad Mukhit Kazi Lecturer SDCH Pune
45. Systemic autoimmune disease
Systemic lupus erythematosus
Rheumatoid arthritis
Polyarteritis nodosa
Sjogran’s sundrome
Mohammad Mukhit Kazi Lecturer SDCH Pune