This document summarizes procedures for bleeding time and coagulation time tests. It describes how to perform a bleeding time test by making small pricks on the ear or arm and using filter paper to wipe away blood every 30 seconds until bleeding stops. The normal bleeding time range is 3 to 5 minutes. It also explains that coagulation tests like APTT and PT are used to assess coagulation factors and can help identify prolonged factors like VIII or VII. Videos are provided to demonstrate bleeding time test procedures.

1. Hematology practical
(Bleeding & Coagulation Times)
By : Osama Al-Zahrani
@OSAMA_Z96

2. Bleeding Time
- Definition :
Test for know if the platelets function are normal ( Primary hemostasis ) .
- Methods :
- Small prick in ear pinna .
- Small prick in mid arm .
- Procedure :
1- Wear the gloves.
2- Wipe the ear pinna or mid arm by alcohol swab.
3- Prick ear pinna or mid arm.
4- Use the filter paper to wipe the blood every 30s until the bleeding stop.
- Result :
- Normal time to stop the bleeding (normal range) from 3 – 5 minutes .
- If the bleeding stop after 5 minutes : prolonged

3. ‫النزف‬ ‫زمن‬
-‫التعريف‬:
‫كانت‬ ‫إذا‬ ‫ما‬ ‫لمعرفة‬ ‫اختبار‬ ‫هو‬‫طبيعية‬ ‫الدموية‬ ‫الصفائح‬.
-‫الطرق‬:
-‫في‬ ‫صغير‬ ‫ثقب‬‫األذن‬ ‫صيوان‬
-‫منتصف‬ ‫في‬ ‫صغير‬ ‫ثقب‬‫الذراع‬
-‫اإلجراءات‬:
1-‫القفازات‬ ‫ارتدي‬.
2-‫الطبية‬ ‫الكحول‬ ‫بمسحة‬ ‫الذراع‬ ‫منتصف‬ ‫أو‬ ‫األذن‬ ‫صيوان‬ ‫امسح‬.
3-‫الذراع‬ ‫منتصف‬ ‫أو‬ ‫األذن‬ ‫صيوان‬ ‫اثقب‬.
4-‫كل‬ ‫بطرفها‬ ‫الدم‬ ‫لمسح‬ ‫الترشيح‬ ‫ورقة‬ ‫استعمل‬30‫النزيف‬ ‫يتوقف‬ ‫حتى‬ ‫ثانية‬.
-‫النتيجة‬:
-‫النزف‬ ‫لتوقف‬ ‫الطبيعي‬ ‫الوقت‬(‫الطبيعي‬ ‫النطاق‬)‫من‬3‫إلى‬5‫دقائق‬.
-‫بعد‬ ‫النزيف‬ ‫يتوقف‬ ‫لم‬ ‫إذا‬5‫مرضية‬ ‫لحالة‬ ‫يشير‬ ‫فهذا‬ ‫دقائق‬(Prolonged)

4. Coagulation or (Clotting) Time
To differentiate between bleeding & coagulation times,
you must do coagulation factors assay or coagulation profile test
- Definition :
Test for know if the factors of platelets are normal .
- PFT-100 (Platelets Function test) : Electronic test .
- Coagulation profile test :
1- Activated Partial Thromboplastin Time [APTT] test :
Assessment for intrinsic factor. if the result of test was prolonged, look for factor 8 VIII
2- Prothrombin time (PT) test:
Assessment for extrinsic factors. if the result of test was prolonged, look for factor 7 VII

5. • Bleeding Time Test part 1
https://www.youtube.com/watch?v=uFO-PQbG-ok&t=2s
• Bleeding Time Test part 2
https://www.youtube.com/watch?v=3zRkXr3UDT4
Video