Plot‐Scale Monitoring to Determine Effectiveness 
of Individual Water Quality Practices
Matthew Helmers
Dean’s Professor, College of Ag. & Life Sciences 
Professor, Dept. of Ag. and Biosystems Eng.
Iowa State University
Department of Agricultural and Biosystems Engineering
NWRF Drainage
Gilmore City DRF
NERF Drainage
COBS
SERF Drainage
Replicated subsurface drainage plots to evaluate performance
of various in-field management practices
Department of Agricultural and
Biosystems Engineering
Plot Sampling Layout
125 ft
50 ft
Perforated border tile -
drains to remote outlet
Flow monitoring sump
(three drain lines in each sump)
Treatment plot
125 ft
50 ft
Perforated border tile -
drains to remote outlet
Flow monitoring sump
(three drain lines in each sump)
Treatment plot
Flow and sampling set-up at Gilmore City site
Timing of Subsurface Drainage
(1990-2011)
Corn-Soybean Rotation 150/160 lb-N/acre Application Rate
Variability in Drainage, Nitrate Concentration
and Nitrate Loss – Weather a Major Driver
Impact of Nitrogen Application Rate
Impact of Application Timing: 2006-14
140 kg-N/ha 168 kg-N/ha
Impact of Application Timing: 2006-14
140 kg-N/ha 168 kg-N/ha
Impact of Nitrogen Source – 168kg-N/ha
Impact of Nitrogen Source – 168 kg-N/ha
Impacts of Cover Crops on Nitrate-N
Concentration in Drainage Water – Gilmore City
~25% Reduction in Nitrate-N Concentration
With Annual Rye Cover Crop
Using Prairie 
Strips to Reduce 
Sediment and 
Nutrient Loss
prairiestrips.org
Watershed Experiment: NSNWR
Neal Smith Prairie
Learning Center
Site 1
Site 2
Site 3
#S
#S
#S
#S
Site 1
Site 2
Site 3
Site 0
%U
Neal Smith
Prairie Learning Center
0 2 4 6 8 10 12 Kilometers
N
EW
S
300 0 300 600 Meters
Walnut Creek Watershed boundary
Refuge boundary
Site History
• Watersheds under primarily bromegrass cover until fall 
2006
• Watershed instrumentation: spring 2005
• Pre‐treatment data collection: 2005 – 2006 field 
seasons
• Treatment establishment: fall 2006 & spring 2007
– Soybean planted in 2007
– Prairie strips sown in July 2007
• No‐till corn‐soybean rotation in cropped areas
reconstructed prairie
corn - soybean row crops, ZERO TILLAGE
Experimental Watershed Treatments
12 watersheds:
Balanced Incomplete Block Design:
3 reps X 4 treatments X 3 blocks
0% 10% 10% 20%
Watershed Characteristics
Size 
(acre)
Slope 
(%)
Location and percent of grass filters* 
Basswood‐1 1.3 7.5 10% at footslope
Basswood‐2 1.2 6.6 5% at footslope and 5% at upslope
Basswood‐3 1.2 6.4 10% at footslope and 10% upslope
Basswood‐4 1.4 8.2 10% at footslope and 10% upslope
Basswood‐5 3.1 8.9 5% at footslope and 5% upslope
Basswood‐6 2.1 10.5 All rowcrops
Interim‐1 7.4 7.7 3.3% at footslope, 3.3% at sideslope, and 
3.3% at upslope
Interim‐2 7.9 6.1 10% at footslope
Interim‐3 1.8 9.3 All rowcrops
Orbweaver‐1 2.9 10.3 10% at footslope
Orbweaver‐2 5.9 6.7 6.7% at footslope, 6.7% at sideslope, and 
6.7% at upslope
Orbweaver‐3 3.1 6.6 All rowcrops
*Percent of grass filters = area of filters / area of watershed
What is unique?
Natural Flow Conditions
Surface Runoff
Monitoring
H-flumes monitor movement of
water, sediment, and nutrients
Precipitation (April‐November)
Surface Runoff 
Sediment Loss in Runoff (2007‐2012)
>95% Reduction 
in sediment 
export from 
watersheds with 
prairie filter strips
Phosphorus Loss in Runoff (2007‐2012)
Zhou et al., 2014
>90% Reduction 
in TP export from 
watersheds with 
prairie filter strips
Total Nitrogen Loss in Runoff (2007‐2011)
Zhou et al., 2014
>90% Reduction 
in TN export from 
watersheds with 
prairie filter strips
Nitrate‐N Loss in Runoff (2007‐2011)
Zhou et al., 2014
Visual Examples (4 inch rain in June 2008)
100% Crop 100% Prairie
10% Prairie
90% Crop
Summary
• In Iowa, on average the majority of drainage
and nitrate-N loss occurs in April-June
• Timing of nitrogen application (fall or early
season sidedress) had little impact on nitrate-N
concentrations in drainage
• In north-central Iowa, winter cereal rye cover
crops reduced nitrate-N concentration in
subsurface drainage by ~25%
• Strategically sited prairie strips hold potential for
reducing surface runoff and loss of sediment
and nutrients with surface runoff

Helmers - Plot-Scale Monitoring