This study investigated the potential for nutrients from a headland-stacked poultry manure pile to move into groundwater at two sites. At one site with shallower groundwater, increased nitrate levels were detected after a large storm event, while no increase was found at the deeper site. The results suggest current regulations for siting manure piles are effective in preventing groundwater impacts, but unusually large storms could pose a risk, especially at locations with shallow groundwater.