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HAEMOPOIESIS & HISTOLOGY
OF BONE MARROW
Radhakrishnan P
Department Of Anatomy
Hadhramout University College Of Medicine
DrRadhakrishnan
How did you get All the things for surveying
External LIFE???
DrRadhakrishnan
How did you get All the things for surveying
Internal LIFE???
DrRadhakrishnan
Fluid Connective tissue
Centrifuge
DrRadhakrishnan
Blood made up of two parts, Formed elements(Erythrocytes,
Platelets, Leukocytes) & Plasma
DrRadhakrishnan
DrRadhakrishnan
DrRadhakrishnan
DrRadhakrishnan
Where did they come from???
Origin, development and Maturation of Blood cells called as
Haemopoiesis
Origin, development and Maturation of Red Blood cells
called as Erythropoiesis
In adult its from BONE MARROW
What about Fetus ? New Born ? Old Age ?
DrRadhakrishnan
IN FETAL LIFE
1. Mesoblastic Stage (upto 2 months)
Mesenchyme of yolk sac.
2. Hepatic Stage (3-6 months)
Liver and also Spleen and lymphoid
organs
3. Myeloid Stage(last 3 months)
Red bone marrow and liver.
Site of Erythropoiesis „
DrRadhakrishnan
IN NEWBORN BABIES, CHILDREN AND ADULTS
1. Up to the age of 20 years:
Red bone marrow of all bones (long bones and all the flat
bones).
2. After the age of 20 years:
Membranous bones like vertebra, sternum, ribs, scapula, iliac bones
and skull bones and from the ends of long bones.
After 20 years of age, the shaft of the long bones becomes yellow
bone marrow because of fat deposition and looses the erythropoietic
function.
DrRadhakrishnan
What is Bone marrow & Where do you find it ???
 Soft pulpy tissue found in marrow cavities of all bones
DrRadhakrishnan
What is Bone marrow & Where do you find it ???
Yellow Marrow
• Presence of
Adipocytes
Red marrow
• Consists of
Stroma,
Hemopoitic
cord,
Sinusoidal
capillaries
DrRadhakrishnan
Bone marrow ( Decalcified section)
Sinusoid StromaHaemopoetic cells
DrRadhakrishnan
OUR LIFE.....
Cell
Tissues
Organs
Systems
Human
Body
Stem cells ???
Stem cells ???
Stem cells are pluripotential cells capable of self-renewal. Some of
their daughter cells form specific, irreversibly differentiated cell types,
and other daughter cells remain stem cells.
DrRadhakrishnan
Two major cell lineages
Myeloid stem cells
Lymphoid stem cells
PLURIPOTANT STEM CELL
PROGENITOR CELLS
PRECURSOR CELLS (Blast)
MATURE CELLS
After My School Studies
Doctor
Teacher / Surgeon / Paediatrician...
Teacher
DrRadhakrishnan
Uncommitted pluripotent Hemopoitic stem cell
Committed pluripotent Hemopoitic stem cell
Lymphoid stem cell Colony forming Blastocyte
Lymphocytes RBC Neutrophil Basophil Eosinophil
Monocyte
Platelet
Colony forming Unit-E Colony forming Unit-GM Colony forming Unit-M
Granulocytes
Lymphoid lineage Myeloid lineage
DrRadhakrishnan
Colony forming Unit-E (CFU-E) / Erythropoiesis
Proerythroblast / Megaloblast
Basophilic erythroblast / Early normoblast
Polychromatophilic erythroblast / Intermediate
Orthochromatophilic erythroblast / Late
normoblast
Reticulocyte
Erythrocyte
5 DAYS
2 DAYS
DrRadhakrishnan
DrRadhakrishnan
Stages of Erythropoiesis Important events
Proerythroblast Synthesis of Hb starts
Early Erythroblast Nuclei disappear
Intermediate Erythroblast Hb starts appearing
Late Erythroblast Nucleus disappears
Reticulocyte Reticulum formed. Cells enter
into capillary from site of
production
Mature RBC Reticulum disappears. Cell attain
Biconcavity
Cells of CFU-E pass through different stages and finally become the matured RBCs.
During these stages four important changes are noticed.
1. Reduction in size of the cell (from the diameter of 25 to 7.2 µ)
2. Disappearance of nucleoli and nucleus
3. Appearance of hemoglobin
4. Change in the staining properties of the cytoplasm. „
CHANGES DURING ERYTHROPOIESIS
DrRadhakrishnan
 Accounts for largest proportion of blood cells (99% of
total number)
 Each erythrocyte is a biconcave disc
 7.2 um in diameter
 Mature erythrocyte lack in nuclei
 Pale red in colour (paler in centre)
 Life span of 120 days
 450000 – 550000 per cubic micro litre of blood
DrRadhakrishnan
Colony forming Unit-GM / Granulopoisis
Azurophillic / primary Granule- lysosomes Specific Granule
DrRadhakrishnan
Colony forming Unit-GM / Granulopoisis
Myeloblast
Promyelocyte
Early Neutrophillic Myelocyte Early Eosinophillic Early Basophillic
Late Neutrophillic Myelocyte Late Eosinophillic Late Basophillic
Neutrophillic Metamyelocyte Eosinophillic Metamyelocyte
Mature Neutrophil Mature Eosinophil Mature Basophil
DrRadhakrishnan
DrRadhakrishnan
DrRadhakrishnan
Neutrophil Eosinophil Basophil
Constitutes in
WBC
60-70% 2- 4% 1%
Nucleus consisting of 2-5
lobes linked by
chromatin
Bilobed nucleus Irregular lobes
Cytoplasm Specific granules
and Azurophillic
granules
Weekly stains
Large elongated
refractile specific
granules stained
by Eosin
Granules contains
Heparin,
Histamine.
Stains
Metachomatically.
Purple in colour
Life span 1-4 days 8-12 days Few hours to days
DrRadhakrishnan
Colony forming Unit-M
Megakaryoblast
Megakaryocytes
Platelets
 Anucleate cell fragments
 Also known as thrombocytes
 20000-400000 per micro litre of blood.
 Appears as clumps
 Peripheral light blue stain & central
purple stain
DrRadhakrishnan
Colony forming Unit-GM / Monocyte
 Euchromatic nucleus
 Oval, Horeshoe, Kidney shaped. Eccentrically placed
 Cytoplasm basophilic
 In electron microscope many Microvilli seen at the cell
surface
DrRadhakrishnan
Lymphoid colony forming cell
Nucleus is :
 Round
 Stains densely
 Surrounded by very narrow rim
of cytoplasm, basophilic.
Lymphoblast
Lymphocytes
T- Lymphocyte B- Lymphocyte
DrRadhakrishnan
DrRadhakrishnan
RBC Neutrophil
Basophil MonocyteLymphocyte
Eosinophil
DrRadhakrishnan
RBC
Platelets
Eosinophil
Neutrophil
Basophil
RBC
Platelets
DrRadhakrishnan
DrRadhakrishnan
REFERENCE VALUE
DrRadhakrishnan
Mail : yellowkrishpt@gmail.com
Face Book : Radha Krishnan
(yellowkrishpt@gmail.com)
WhatsApp : +91 9003502218
Local : 738912880
Feed back Inv
DrRadhakrishnan

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Haemopoisis and Histology of Bone Marrow

  • 1. HAEMOPOIESIS & HISTOLOGY OF BONE MARROW Radhakrishnan P Department Of Anatomy Hadhramout University College Of Medicine DrRadhakrishnan
  • 2. How did you get All the things for surveying External LIFE??? DrRadhakrishnan
  • 3. How did you get All the things for surveying Internal LIFE??? DrRadhakrishnan
  • 5. Blood made up of two parts, Formed elements(Erythrocytes, Platelets, Leukocytes) & Plasma DrRadhakrishnan
  • 9. Where did they come from??? Origin, development and Maturation of Blood cells called as Haemopoiesis Origin, development and Maturation of Red Blood cells called as Erythropoiesis In adult its from BONE MARROW What about Fetus ? New Born ? Old Age ? DrRadhakrishnan
  • 10. IN FETAL LIFE 1. Mesoblastic Stage (upto 2 months) Mesenchyme of yolk sac. 2. Hepatic Stage (3-6 months) Liver and also Spleen and lymphoid organs 3. Myeloid Stage(last 3 months) Red bone marrow and liver. Site of Erythropoiesis „ DrRadhakrishnan
  • 11. IN NEWBORN BABIES, CHILDREN AND ADULTS 1. Up to the age of 20 years: Red bone marrow of all bones (long bones and all the flat bones). 2. After the age of 20 years: Membranous bones like vertebra, sternum, ribs, scapula, iliac bones and skull bones and from the ends of long bones. After 20 years of age, the shaft of the long bones becomes yellow bone marrow because of fat deposition and looses the erythropoietic function. DrRadhakrishnan
  • 12. What is Bone marrow & Where do you find it ???  Soft pulpy tissue found in marrow cavities of all bones DrRadhakrishnan
  • 13. What is Bone marrow & Where do you find it ??? Yellow Marrow • Presence of Adipocytes Red marrow • Consists of Stroma, Hemopoitic cord, Sinusoidal capillaries DrRadhakrishnan
  • 14. Bone marrow ( Decalcified section) Sinusoid StromaHaemopoetic cells DrRadhakrishnan
  • 16. Stem cells ??? Stem cells are pluripotential cells capable of self-renewal. Some of their daughter cells form specific, irreversibly differentiated cell types, and other daughter cells remain stem cells. DrRadhakrishnan
  • 17. Two major cell lineages Myeloid stem cells Lymphoid stem cells PLURIPOTANT STEM CELL PROGENITOR CELLS PRECURSOR CELLS (Blast) MATURE CELLS After My School Studies Doctor Teacher / Surgeon / Paediatrician... Teacher DrRadhakrishnan
  • 18. Uncommitted pluripotent Hemopoitic stem cell Committed pluripotent Hemopoitic stem cell Lymphoid stem cell Colony forming Blastocyte Lymphocytes RBC Neutrophil Basophil Eosinophil Monocyte Platelet Colony forming Unit-E Colony forming Unit-GM Colony forming Unit-M Granulocytes Lymphoid lineage Myeloid lineage DrRadhakrishnan
  • 19. Colony forming Unit-E (CFU-E) / Erythropoiesis Proerythroblast / Megaloblast Basophilic erythroblast / Early normoblast Polychromatophilic erythroblast / Intermediate Orthochromatophilic erythroblast / Late normoblast Reticulocyte Erythrocyte 5 DAYS 2 DAYS DrRadhakrishnan
  • 21. Stages of Erythropoiesis Important events Proerythroblast Synthesis of Hb starts Early Erythroblast Nuclei disappear Intermediate Erythroblast Hb starts appearing Late Erythroblast Nucleus disappears Reticulocyte Reticulum formed. Cells enter into capillary from site of production Mature RBC Reticulum disappears. Cell attain Biconcavity Cells of CFU-E pass through different stages and finally become the matured RBCs. During these stages four important changes are noticed. 1. Reduction in size of the cell (from the diameter of 25 to 7.2 µ) 2. Disappearance of nucleoli and nucleus 3. Appearance of hemoglobin 4. Change in the staining properties of the cytoplasm. „ CHANGES DURING ERYTHROPOIESIS DrRadhakrishnan
  • 22.  Accounts for largest proportion of blood cells (99% of total number)  Each erythrocyte is a biconcave disc  7.2 um in diameter  Mature erythrocyte lack in nuclei  Pale red in colour (paler in centre)  Life span of 120 days  450000 – 550000 per cubic micro litre of blood DrRadhakrishnan
  • 23. Colony forming Unit-GM / Granulopoisis Azurophillic / primary Granule- lysosomes Specific Granule DrRadhakrishnan
  • 24. Colony forming Unit-GM / Granulopoisis Myeloblast Promyelocyte Early Neutrophillic Myelocyte Early Eosinophillic Early Basophillic Late Neutrophillic Myelocyte Late Eosinophillic Late Basophillic Neutrophillic Metamyelocyte Eosinophillic Metamyelocyte Mature Neutrophil Mature Eosinophil Mature Basophil DrRadhakrishnan
  • 27. Neutrophil Eosinophil Basophil Constitutes in WBC 60-70% 2- 4% 1% Nucleus consisting of 2-5 lobes linked by chromatin Bilobed nucleus Irregular lobes Cytoplasm Specific granules and Azurophillic granules Weekly stains Large elongated refractile specific granules stained by Eosin Granules contains Heparin, Histamine. Stains Metachomatically. Purple in colour Life span 1-4 days 8-12 days Few hours to days DrRadhakrishnan
  • 28. Colony forming Unit-M Megakaryoblast Megakaryocytes Platelets  Anucleate cell fragments  Also known as thrombocytes  20000-400000 per micro litre of blood.  Appears as clumps  Peripheral light blue stain & central purple stain DrRadhakrishnan
  • 29. Colony forming Unit-GM / Monocyte  Euchromatic nucleus  Oval, Horeshoe, Kidney shaped. Eccentrically placed  Cytoplasm basophilic  In electron microscope many Microvilli seen at the cell surface DrRadhakrishnan
  • 30. Lymphoid colony forming cell Nucleus is :  Round  Stains densely  Surrounded by very narrow rim of cytoplasm, basophilic. Lymphoblast Lymphocytes T- Lymphocyte B- Lymphocyte DrRadhakrishnan
  • 36. Mail : yellowkrishpt@gmail.com Face Book : Radha Krishnan (yellowkrishpt@gmail.com) WhatsApp : +91 9003502218 Local : 738912880 Feed back Inv DrRadhakrishnan