SlideShare a Scribd company logo
Januar Natanael, Rizkita Rachmi Esyanti,Robert Manurung
Department of Biological Engineering
School of Life Sciences and Technology
Institut Teknologi Bandung, Indonesia

 Eurycoma longifolia Jack or tongkat ali can be used as a
medicine to cure malaria, anti-inflammatory, anti-
microbial, aphrodisiac and is still being developed as
anticancer (Bhat, et al., 2010)
Problem:
 excess exploitation of this plants  critically endangered
(Sudiarto, et al., 2002)
Alternative method  tissue culture technique
Introduction

 Elicitation is a treatment given to culture, such as by
exposing ultra-violet light, to induce the production
of specific metabolites
 UV elicitation has been proved able to induce the
formation of the enzyme tryptophan decarboxylase
on Catharantus roseus L.  alkaloid production
increased (Ouwerkerk et al., 1999)
Introduction

 Biomass propagation in the bioreactor is a way to
increase the biomass and secondary metabolites
production in a larger scale.
 There are several factors that must be considered in
the scale-up process, such as: profiles of stirring, the
solubility of oxygen in the medium, the exact
dimensions of the reactor, the size of the cells used,
and many more (Taticek et al., 1991)
Introduction

 In this study, the effect of scale-up of cell suspension
cultures of E. longifolia in bioreactor conditions and
treated by UV light on the growth and secondary
metabolite production will be evaluated.
Objective

Methods
Subcultivated
callus in fresh
solid media
Initiation
cell
suspension
from callus
Next
MS Medium half strength
1,1 ppm 2,4-D and 1 ppm kinetin
MS Solid Medium half stregth
2,2 ppm 2,4-D and 2 ppm kinetin

Methods
Subcultured cell
aggregate into
fresh liquid and
bioreactor for
treatment
Analysis
Treatment:
Exposed by UV 18
hr/day
Rotation: 100 rpm
Air flow rate: 0.3 vvm

E. longifolia Cell Culture
Specific Growth Rate
y = 0.0257x + 0.0468
R² = 0.9129
y = 0.0225x - 0.019
R² = 0.9316
y = 0.0549x
-0.1
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
0 2 4 6 8 10 12 14
ln(Xt/Xo)
Day
Flask-Control
Flask-UV
Bioreactor-UV
*
* Ouwerkerk et al., 1999 dan Stephanopoulus et al., 1998).
** Shuler dan Kargi, 2002 dan Schlatmann et al., 1994
**

Medium Conductivity Profile
of E. longifolia Cell Culture
2
2.5
3
3.5
4
4.5
5
5.5
6
0 2 4 6 8 10 12 14
Conductivity(mS)
Day
Flask-Control
Flask-UV
Bioreactor-UV
*Taya et al., 1989 dan Hahlbrock, 1975

Sucrose Consumption of
E. longifolia Cell Culture
y = -0.56x + 24.86
R² = 0.99145
y = -0.43x + 25.34
R² = 0.91009
y = -0.18x + 25
13.00
15.00
17.00
19.00
21.00
23.00
25.00
27.00
-1 4 9 14
SucroseLevel(g/L)
Day
Flask-Control
Flask-UV
Bioreactor-UV
* Shuler dan Kargi, 2002
** Shuler dan Kargi, 2002
**
*

Secondary Metabolites of
E. longifolia Cell Culture
Day
Metabolites in Control
Culture
Amount Day
Metabolites in UV-Exposed
Culture
Amount Day
Metabolites in UV-
Exposed Bioreactor
Amount
9 Eurylactone 3.75% 4 Squalene 4.38% 14 Canthin-6-one 6.81%
12
5-(hydroxymethyl)-2-
Furancarboxaldehyde
20.52% 9 Squalene 8.04% 14 Beta-carboline 29.24%
14 Canthin-6-one 2.67% 12 Quasinoid 4.03%
14 Squalene 3.50% 14 Canthin-6-one 5.12%
14 Beta-carboline 25.11%
* Arvy et al., 1994; Fett-Neto dan Zhang, 1994; dan Ouwerkerk et al., 1999

 E. longifolia cell cultures that were grown in
bioreactor had a faster growth rate than in the flask
culture.
 UV light inhibits the growth of E. longifolia cell
culture
 The faster the rate of biomass formation, the faster
the rate of sucrose and mineral salts consumption.
 Elicitation with UV light increases the production of
secondary metabolites in both the flask scale culture
and bioreactor scale culture.
Conclusions

Thank You

More Related Content

What's hot

Abbas Morovvati
Abbas MorovvatiAbbas Morovvati
Abbas Morovvati
abbasmorovvati
 
Nanotechnology in Livestock and Poultry Feeding
Nanotechnology in Livestock and Poultry FeedingNanotechnology in Livestock and Poultry Feeding
Senior seminar- Nanotechnology in Agriculture
Senior seminar- Nanotechnology in AgricultureSenior seminar- Nanotechnology in Agriculture
Senior seminar- Nanotechnology in Agriculture
Solomon Etany
 
Nano pesticide: An Emerging Tool for Pest Magagement
Nano pesticide: An Emerging Tool for Pest MagagementNano pesticide: An Emerging Tool for Pest Magagement
Nano pesticide: An Emerging Tool for Pest Magagement
Gopal Golvankar
 
"Nanotechnology in agriculture and Food technology" By MOHD. HASHIM KHAN
"Nanotechnology in agriculture and Food technology" By MOHD. HASHIM KHAN"Nanotechnology in agriculture and Food technology" By MOHD. HASHIM KHAN
"Nanotechnology in agriculture and Food technology" By MOHD. HASHIM KHAN
Mohd. Hashim Khan
 
Nano technology in Agriculture
Nano technology in AgricultureNano technology in Agriculture
Nano technology in Agriculture
ranganihennayaka
 
Controlled release formulations as a smart delivery system for eco friendly p...
Controlled release formulations as a smart delivery system for eco friendly p...Controlled release formulations as a smart delivery system for eco friendly p...
Controlled release formulations as a smart delivery system for eco friendly p...
Asst Prof SSNAIK ENTO PJTSAU
 
Nanotechnology in agriculture
Nanotechnology in agricultureNanotechnology in agriculture
Nanotechnology in agriculture
Nikunj Aggarwal
 
Final gokul seminar
Final gokul seminarFinal gokul seminar
Final gokul seminar
GOKULCP1
 
Nanotechnology in aquaculture
Nanotechnology in aquacultureNanotechnology in aquaculture
Nanotechnology in aquaculture
Romen Wangkheirakpam
 
Synthetic Biology (Names in Group)
Synthetic Biology (Names in Group)Synthetic Biology (Names in Group)
Synthetic Biology (Names in Group)
John Cork
 
role of nanotechnology for crop protection in horticultural crops
role of nanotechnology for crop protection in horticultural cropsrole of nanotechnology for crop protection in horticultural crops
role of nanotechnology for crop protection in horticultural crops
girija kumari
 
Nanotechnology and applications in agriculture
Nanotechnology and applications in agricultureNanotechnology and applications in agriculture
Nanotechnology and applications in agriculture
Yahya Alyasiri
 
NANOTECHNOLOGY IN AGRICULTURE
NANOTECHNOLOGY IN AGRICULTURENANOTECHNOLOGY IN AGRICULTURE
NANOTECHNOLOGY IN AGRICULTURE
KrishnaVenir6
 
Application of nanotechnology in agriculture
Application of nanotechnology in agricultureApplication of nanotechnology in agriculture
Application of nanotechnology in agriculture
Amit Bishnoi
 
Agriculture applications of nanobiotechnology
Agriculture applications of nanobiotechnologyAgriculture applications of nanobiotechnology
Agriculture applications of nanobiotechnology
Sabahat Ali
 
Nanotechnology and its Applications in Agriculture
Nanotechnology and its Applications in AgricultureNanotechnology and its Applications in Agriculture
Nanotechnology and its Applications in Agriculture
Younus Fayaz
 
Role of nanotechnology in insect pest management
Role of nanotechnology in insect pest managementRole of nanotechnology in insect pest management
Role of nanotechnology in insect pest management
bajaru
 
Nanotechnology in aquaculture ppt
Nanotechnology in aquaculture pptNanotechnology in aquaculture ppt
Nanotechnology in aquaculture ppt
College of Fisheries Mangaluru
 
Combined effect of solar drying and gamma radiation on the microbiological qu...
Combined effect of solar drying and gamma radiation on the microbiological qu...Combined effect of solar drying and gamma radiation on the microbiological qu...
Combined effect of solar drying and gamma radiation on the microbiological qu...
Nii Korley Kortei
 

What's hot (20)

Abbas Morovvati
Abbas MorovvatiAbbas Morovvati
Abbas Morovvati
 
Nanotechnology in Livestock and Poultry Feeding
Nanotechnology in Livestock and Poultry FeedingNanotechnology in Livestock and Poultry Feeding
Nanotechnology in Livestock and Poultry Feeding
 
Senior seminar- Nanotechnology in Agriculture
Senior seminar- Nanotechnology in AgricultureSenior seminar- Nanotechnology in Agriculture
Senior seminar- Nanotechnology in Agriculture
 
Nano pesticide: An Emerging Tool for Pest Magagement
Nano pesticide: An Emerging Tool for Pest MagagementNano pesticide: An Emerging Tool for Pest Magagement
Nano pesticide: An Emerging Tool for Pest Magagement
 
"Nanotechnology in agriculture and Food technology" By MOHD. HASHIM KHAN
"Nanotechnology in agriculture and Food technology" By MOHD. HASHIM KHAN"Nanotechnology in agriculture and Food technology" By MOHD. HASHIM KHAN
"Nanotechnology in agriculture and Food technology" By MOHD. HASHIM KHAN
 
Nano technology in Agriculture
Nano technology in AgricultureNano technology in Agriculture
Nano technology in Agriculture
 
Controlled release formulations as a smart delivery system for eco friendly p...
Controlled release formulations as a smart delivery system for eco friendly p...Controlled release formulations as a smart delivery system for eco friendly p...
Controlled release formulations as a smart delivery system for eco friendly p...
 
Nanotechnology in agriculture
Nanotechnology in agricultureNanotechnology in agriculture
Nanotechnology in agriculture
 
Final gokul seminar
Final gokul seminarFinal gokul seminar
Final gokul seminar
 
Nanotechnology in aquaculture
Nanotechnology in aquacultureNanotechnology in aquaculture
Nanotechnology in aquaculture
 
Synthetic Biology (Names in Group)
Synthetic Biology (Names in Group)Synthetic Biology (Names in Group)
Synthetic Biology (Names in Group)
 
role of nanotechnology for crop protection in horticultural crops
role of nanotechnology for crop protection in horticultural cropsrole of nanotechnology for crop protection in horticultural crops
role of nanotechnology for crop protection in horticultural crops
 
Nanotechnology and applications in agriculture
Nanotechnology and applications in agricultureNanotechnology and applications in agriculture
Nanotechnology and applications in agriculture
 
NANOTECHNOLOGY IN AGRICULTURE
NANOTECHNOLOGY IN AGRICULTURENANOTECHNOLOGY IN AGRICULTURE
NANOTECHNOLOGY IN AGRICULTURE
 
Application of nanotechnology in agriculture
Application of nanotechnology in agricultureApplication of nanotechnology in agriculture
Application of nanotechnology in agriculture
 
Agriculture applications of nanobiotechnology
Agriculture applications of nanobiotechnologyAgriculture applications of nanobiotechnology
Agriculture applications of nanobiotechnology
 
Nanotechnology and its Applications in Agriculture
Nanotechnology and its Applications in AgricultureNanotechnology and its Applications in Agriculture
Nanotechnology and its Applications in Agriculture
 
Role of nanotechnology in insect pest management
Role of nanotechnology in insect pest managementRole of nanotechnology in insect pest management
Role of nanotechnology in insect pest management
 
Nanotechnology in aquaculture ppt
Nanotechnology in aquaculture pptNanotechnology in aquaculture ppt
Nanotechnology in aquaculture ppt
 
Combined effect of solar drying and gamma radiation on the microbiological qu...
Combined effect of solar drying and gamma radiation on the microbiological qu...Combined effect of solar drying and gamma radiation on the microbiological qu...
Combined effect of solar drying and gamma radiation on the microbiological qu...
 

Viewers also liked

Grds international conference on social science (2)
Grds international conference on social science (2)Grds international conference on social science (2)
Grds international conference on social science (2)
Global R & D Services
 
Ictel 2015 (daebum, jung) GRDS International Conferences
Ictel 2015 (daebum, jung) GRDS International ConferencesIctel 2015 (daebum, jung) GRDS International Conferences
Ictel 2015 (daebum, jung) GRDS International Conferences
Global R & D Services
 
Trolan presentation
Trolan presentationTrolan presentation
Trolan presentation
Global R & D Services
 
Grds conferences icst and icbelsh (6)
Grds conferences icst and icbelsh (6)Grds conferences icst and icbelsh (6)
Grds conferences icst and icbelsh (6)
Global R & D Services
 
Grds conferences icst and icbelsh (10)
Grds conferences icst and icbelsh (10)Grds conferences icst and icbelsh (10)
Grds conferences icst and icbelsh (10)
Global R & D Services
 
Grds international conference on health and life (14)
Grds international conference on health and life (14)Grds international conference on health and life (14)
Grds international conference on health and life (14)
Global R & D Services
 
Grds international conference on business and management (4)
Grds international conference on business and management (4)Grds international conference on business and management (4)
Grds international conference on business and management (4)
Global R & D Services
 
Grds international conference on business and management (2)
Grds international conference on business and management (2)Grds international conference on business and management (2)
Grds international conference on business and management (2)
Global R & D Services
 
ICRST proceedings of Novemeber 2016,Singapore
ICRST proceedings of Novemeber 2016,Singapore ICRST proceedings of Novemeber 2016,Singapore
ICRST proceedings of Novemeber 2016,Singapore
Global R & D Services
 
ECG proceedings of November 2016,Singapore
ECG proceedings of November 2016,SingaporeECG proceedings of November 2016,Singapore
ECG proceedings of November 2016,Singapore
Global R & D Services
 
Grds international conference on health and life (4)
Grds international conference on health and life (4)Grds international conference on health and life (4)
Grds international conference on health and life (4)
Global R & D Services
 
Grds international conference on health and life (2)
Grds international conference on health and life (2)Grds international conference on health and life (2)
Grds international conference on health and life (2)
Global R & D Services
 
HNDM proceedings of November 2016,Singapore
HNDM proceedings of November 2016,SingaporeHNDM proceedings of November 2016,Singapore
HNDM proceedings of November 2016,Singapore
Global R & D Services
 

Viewers also liked (13)

Grds international conference on social science (2)
Grds international conference on social science (2)Grds international conference on social science (2)
Grds international conference on social science (2)
 
Ictel 2015 (daebum, jung) GRDS International Conferences
Ictel 2015 (daebum, jung) GRDS International ConferencesIctel 2015 (daebum, jung) GRDS International Conferences
Ictel 2015 (daebum, jung) GRDS International Conferences
 
Trolan presentation
Trolan presentationTrolan presentation
Trolan presentation
 
Grds conferences icst and icbelsh (6)
Grds conferences icst and icbelsh (6)Grds conferences icst and icbelsh (6)
Grds conferences icst and icbelsh (6)
 
Grds conferences icst and icbelsh (10)
Grds conferences icst and icbelsh (10)Grds conferences icst and icbelsh (10)
Grds conferences icst and icbelsh (10)
 
Grds international conference on health and life (14)
Grds international conference on health and life (14)Grds international conference on health and life (14)
Grds international conference on health and life (14)
 
Grds international conference on business and management (4)
Grds international conference on business and management (4)Grds international conference on business and management (4)
Grds international conference on business and management (4)
 
Grds international conference on business and management (2)
Grds international conference on business and management (2)Grds international conference on business and management (2)
Grds international conference on business and management (2)
 
ICRST proceedings of Novemeber 2016,Singapore
ICRST proceedings of Novemeber 2016,Singapore ICRST proceedings of Novemeber 2016,Singapore
ICRST proceedings of Novemeber 2016,Singapore
 
ECG proceedings of November 2016,Singapore
ECG proceedings of November 2016,SingaporeECG proceedings of November 2016,Singapore
ECG proceedings of November 2016,Singapore
 
Grds international conference on health and life (4)
Grds international conference on health and life (4)Grds international conference on health and life (4)
Grds international conference on health and life (4)
 
Grds international conference on health and life (2)
Grds international conference on health and life (2)Grds international conference on health and life (2)
Grds international conference on health and life (2)
 
HNDM proceedings of November 2016,Singapore
HNDM proceedings of November 2016,SingaporeHNDM proceedings of November 2016,Singapore
HNDM proceedings of November 2016,Singapore
 

Similar to Grds international conference on health and life (8)

The effects of ultraviolet light emitting diodes with different wavelengths o...
The effects of ultraviolet light emitting diodes with different wavelengths o...The effects of ultraviolet light emitting diodes with different wavelengths o...
The effects of ultraviolet light emitting diodes with different wavelengths o...
Nay Aung
 
Modeling the solar drying kinetics of gamma irradiation-pretreated oyster mus...
Modeling the solar drying kinetics of gamma irradiation-pretreated oyster mus...Modeling the solar drying kinetics of gamma irradiation-pretreated oyster mus...
Modeling the solar drying kinetics of gamma irradiation-pretreated oyster mus...
Nii Korley Kortei
 
37_8_Isolation
37_8_Isolation37_8_Isolation
37_8_Isolation
Pranay Abhang
 
Nii korley kortei (sorghum)
Nii korley kortei (sorghum)Nii korley kortei (sorghum)
Nii korley kortei (sorghum)
Nii Korley Kortei
 
Evaluation of Protective Efficacy of Hydro Alcoholic Extract and Methanol Fra...
Evaluation of Protective Efficacy of Hydro Alcoholic Extract and Methanol Fra...Evaluation of Protective Efficacy of Hydro Alcoholic Extract and Methanol Fra...
Evaluation of Protective Efficacy of Hydro Alcoholic Extract and Methanol Fra...
paperpublications3
 
Effects of gamma rays on reproductive traits of Vigna vexillata (L.) A. Rich.
Effects of gamma rays on reproductive traits of Vigna vexillata (L.) A. Rich.Effects of gamma rays on reproductive traits of Vigna vexillata (L.) A. Rich.
Effects of gamma rays on reproductive traits of Vigna vexillata (L.) A. Rich.
International Institute of Tropical Agriculture
 
biochem.pptx
biochem.pptxbiochem.pptx
biochem.pptx
parmarsneha2
 
Development of a rapid, effective method for seeding biofiltration systems us...
Development of a rapid, effective method for seeding biofiltration systems us...Development of a rapid, effective method for seeding biofiltration systems us...
Development of a rapid, effective method for seeding biofiltration systems us...
osilblossom
 
Biodegradable Nanoparticles For Drug Delivery
Biodegradable Nanoparticles For Drug DeliveryBiodegradable Nanoparticles For Drug Delivery
Biodegradable Nanoparticles For Drug Delivery
Amber Wheeler
 
Role of Copper and Zinc Nanoparticles in Plant Disease Management
Role of Copper and Zinc Nanoparticles in Plant Disease ManagementRole of Copper and Zinc Nanoparticles in Plant Disease Management
Role of Copper and Zinc Nanoparticles in Plant Disease Management
Ravikumar Vaniya
 
life sci paper
life sci paperlife sci paper
life sci paper
Vanitha Vinodkumar
 
Food_irradiaton_Basics.pptx
Food_irradiaton_Basics.pptxFood_irradiaton_Basics.pptx
Food_irradiaton_Basics.pptx
Leena Kumari
 
Seminar plantlet response to seismomorphogenic stimuli (mechanical stress med...
Seminar plantlet response to seismomorphogenic stimuli (mechanical stress med...Seminar plantlet response to seismomorphogenic stimuli (mechanical stress med...
Seminar plantlet response to seismomorphogenic stimuli (mechanical stress med...
Daya Singh
 
20200504-philips-uv-purification-application-information.pdf
20200504-philips-uv-purification-application-information.pdf20200504-philips-uv-purification-application-information.pdf
20200504-philips-uv-purification-application-information.pdf
Tien Din Tran
 
Ultravoilet radiation as a non-thermal treatment for inactivation of microorg...
Ultravoilet radiation as a non-thermal treatment for inactivation of microorg...Ultravoilet radiation as a non-thermal treatment for inactivation of microorg...
Ultravoilet radiation as a non-thermal treatment for inactivation of microorg...
Maya Sharma
 
Inductive Toxic-Mopping (IT-m) and Carotenogenic Bioconversion Properties of ...
Inductive Toxic-Mopping (IT-m) and Carotenogenic Bioconversion Properties of ...Inductive Toxic-Mopping (IT-m) and Carotenogenic Bioconversion Properties of ...
Inductive Toxic-Mopping (IT-m) and Carotenogenic Bioconversion Properties of ...
iosrjce
 
Mutagenic Effects of Ultraviolet Radiation on Growth and Agronomic Characters...
Mutagenic Effects of Ultraviolet Radiation on Growth and Agronomic Characters...Mutagenic Effects of Ultraviolet Radiation on Growth and Agronomic Characters...
Mutagenic Effects of Ultraviolet Radiation on Growth and Agronomic Characters...
Professor Bashir Omolaran Bello
 
Innovative Plant for the Production of Microalgaes
Innovative Plant for the Production of MicroalgaesInnovative Plant for the Production of Microalgaes
Innovative Plant for the Production of Microalgaes
Associate Professor in VSB Coimbatore
 
Acute toxicity study and effects of sesame (Sesamum radiatum) aqueous leaf ex...
Acute toxicity study and effects of sesame (Sesamum radiatum) aqueous leaf ex...Acute toxicity study and effects of sesame (Sesamum radiatum) aqueous leaf ex...
Acute toxicity study and effects of sesame (Sesamum radiatum) aqueous leaf ex...
Open Access Research Paper
 
Bioprospeksi mikroorganisme ekstremofilik.pptx
Bioprospeksi mikroorganisme ekstremofilik.pptxBioprospeksi mikroorganisme ekstremofilik.pptx
Bioprospeksi mikroorganisme ekstremofilik.pptx
BerrycheeseCake
 

Similar to Grds international conference on health and life (8) (20)

The effects of ultraviolet light emitting diodes with different wavelengths o...
The effects of ultraviolet light emitting diodes with different wavelengths o...The effects of ultraviolet light emitting diodes with different wavelengths o...
The effects of ultraviolet light emitting diodes with different wavelengths o...
 
Modeling the solar drying kinetics of gamma irradiation-pretreated oyster mus...
Modeling the solar drying kinetics of gamma irradiation-pretreated oyster mus...Modeling the solar drying kinetics of gamma irradiation-pretreated oyster mus...
Modeling the solar drying kinetics of gamma irradiation-pretreated oyster mus...
 
37_8_Isolation
37_8_Isolation37_8_Isolation
37_8_Isolation
 
Nii korley kortei (sorghum)
Nii korley kortei (sorghum)Nii korley kortei (sorghum)
Nii korley kortei (sorghum)
 
Evaluation of Protective Efficacy of Hydro Alcoholic Extract and Methanol Fra...
Evaluation of Protective Efficacy of Hydro Alcoholic Extract and Methanol Fra...Evaluation of Protective Efficacy of Hydro Alcoholic Extract and Methanol Fra...
Evaluation of Protective Efficacy of Hydro Alcoholic Extract and Methanol Fra...
 
Effects of gamma rays on reproductive traits of Vigna vexillata (L.) A. Rich.
Effects of gamma rays on reproductive traits of Vigna vexillata (L.) A. Rich.Effects of gamma rays on reproductive traits of Vigna vexillata (L.) A. Rich.
Effects of gamma rays on reproductive traits of Vigna vexillata (L.) A. Rich.
 
biochem.pptx
biochem.pptxbiochem.pptx
biochem.pptx
 
Development of a rapid, effective method for seeding biofiltration systems us...
Development of a rapid, effective method for seeding biofiltration systems us...Development of a rapid, effective method for seeding biofiltration systems us...
Development of a rapid, effective method for seeding biofiltration systems us...
 
Biodegradable Nanoparticles For Drug Delivery
Biodegradable Nanoparticles For Drug DeliveryBiodegradable Nanoparticles For Drug Delivery
Biodegradable Nanoparticles For Drug Delivery
 
Role of Copper and Zinc Nanoparticles in Plant Disease Management
Role of Copper and Zinc Nanoparticles in Plant Disease ManagementRole of Copper and Zinc Nanoparticles in Plant Disease Management
Role of Copper and Zinc Nanoparticles in Plant Disease Management
 
life sci paper
life sci paperlife sci paper
life sci paper
 
Food_irradiaton_Basics.pptx
Food_irradiaton_Basics.pptxFood_irradiaton_Basics.pptx
Food_irradiaton_Basics.pptx
 
Seminar plantlet response to seismomorphogenic stimuli (mechanical stress med...
Seminar plantlet response to seismomorphogenic stimuli (mechanical stress med...Seminar plantlet response to seismomorphogenic stimuli (mechanical stress med...
Seminar plantlet response to seismomorphogenic stimuli (mechanical stress med...
 
20200504-philips-uv-purification-application-information.pdf
20200504-philips-uv-purification-application-information.pdf20200504-philips-uv-purification-application-information.pdf
20200504-philips-uv-purification-application-information.pdf
 
Ultravoilet radiation as a non-thermal treatment for inactivation of microorg...
Ultravoilet radiation as a non-thermal treatment for inactivation of microorg...Ultravoilet radiation as a non-thermal treatment for inactivation of microorg...
Ultravoilet radiation as a non-thermal treatment for inactivation of microorg...
 
Inductive Toxic-Mopping (IT-m) and Carotenogenic Bioconversion Properties of ...
Inductive Toxic-Mopping (IT-m) and Carotenogenic Bioconversion Properties of ...Inductive Toxic-Mopping (IT-m) and Carotenogenic Bioconversion Properties of ...
Inductive Toxic-Mopping (IT-m) and Carotenogenic Bioconversion Properties of ...
 
Mutagenic Effects of Ultraviolet Radiation on Growth and Agronomic Characters...
Mutagenic Effects of Ultraviolet Radiation on Growth and Agronomic Characters...Mutagenic Effects of Ultraviolet Radiation on Growth and Agronomic Characters...
Mutagenic Effects of Ultraviolet Radiation on Growth and Agronomic Characters...
 
Innovative Plant for the Production of Microalgaes
Innovative Plant for the Production of MicroalgaesInnovative Plant for the Production of Microalgaes
Innovative Plant for the Production of Microalgaes
 
Acute toxicity study and effects of sesame (Sesamum radiatum) aqueous leaf ex...
Acute toxicity study and effects of sesame (Sesamum radiatum) aqueous leaf ex...Acute toxicity study and effects of sesame (Sesamum radiatum) aqueous leaf ex...
Acute toxicity study and effects of sesame (Sesamum radiatum) aqueous leaf ex...
 
Bioprospeksi mikroorganisme ekstremofilik.pptx
Bioprospeksi mikroorganisme ekstremofilik.pptxBioprospeksi mikroorganisme ekstremofilik.pptx
Bioprospeksi mikroorganisme ekstremofilik.pptx
 

More from Global R & D Services

Wb june ictel
Wb june ictelWb june ictel
Wb june ictel
Global R & D Services
 
Wb june icrst
Wb june icrstWb june icrst
Wb june icrst
Global R & D Services
 
Wb june ecg
Wb june ecgWb june ecg
Wb june icssh
Wb  june icsshWb  june icssh
Wb june icssh
Global R & D Services
 
Wb june icpbs
Wb  june icpbsWb  june icpbs
Wb june icpbs
Global R & D Services
 
Wb june icnm
Wb  june icnmWb  june icnm
Wb june icllr
Wb  june icllrWb  june icllr
Wb june icllr
Global R & D Services
 
Wb june ichlsr
Wb  june ichlsrWb  june ichlsr
Wb june ichlsr
Global R & D Services
 
Wb june icbmls
Wb  june icbmlsWb  june icbmls
Wb june icbmls
Global R & D Services
 
Rome icpbs 2017
Rome icpbs 2017Rome icpbs 2017
Rome icpbs 2017
Global R & D Services
 
Rome icnm
Rome icnmRome icnm
Romei ecg 2017
Romei ecg 2017Romei ecg 2017
Romei ecg 2017
Global R & D Services
 
Rome ictel 2017
Rome  ictel 2017Rome  ictel 2017
Rome ictel 2017
Global R & D Services
 
Rome icssh, ppt
Rome icssh, pptRome icssh, ppt
Rome icssh, ppt
Global R & D Services
 
Rome icrst 2017
Rome  icrst 2017Rome  icrst 2017
Rome icrst 2017
Global R & D Services
 
Rome icllr 2017
Rome  icllr 2017Rome  icllr 2017
Rome icllr 2017
Global R & D Services
 
Rome ichlsr
Rome  ichlsr Rome  ichlsr
Rome icbmls 2017
Rome icbmls 2017Rome icbmls 2017
Rome icbmls 2017
Global R & D Services
 
Wb june ictel
Wb june ictelWb june ictel
Wb june ictel
Global R & D Services
 
Wb june icrst
Wb june icrstWb june icrst
Wb june icrst
Global R & D Services
 

More from Global R & D Services (20)

Wb june ictel
Wb june ictelWb june ictel
Wb june ictel
 
Wb june icrst
Wb june icrstWb june icrst
Wb june icrst
 
Wb june ecg
Wb june ecgWb june ecg
Wb june ecg
 
Wb june icssh
Wb  june icsshWb  june icssh
Wb june icssh
 
Wb june icpbs
Wb  june icpbsWb  june icpbs
Wb june icpbs
 
Wb june icnm
Wb  june icnmWb  june icnm
Wb june icnm
 
Wb june icllr
Wb  june icllrWb  june icllr
Wb june icllr
 
Wb june ichlsr
Wb  june ichlsrWb  june ichlsr
Wb june ichlsr
 
Wb june icbmls
Wb  june icbmlsWb  june icbmls
Wb june icbmls
 
Rome icpbs 2017
Rome icpbs 2017Rome icpbs 2017
Rome icpbs 2017
 
Rome icnm
Rome icnmRome icnm
Rome icnm
 
Romei ecg 2017
Romei ecg 2017Romei ecg 2017
Romei ecg 2017
 
Rome ictel 2017
Rome  ictel 2017Rome  ictel 2017
Rome ictel 2017
 
Rome icssh, ppt
Rome icssh, pptRome icssh, ppt
Rome icssh, ppt
 
Rome icrst 2017
Rome  icrst 2017Rome  icrst 2017
Rome icrst 2017
 
Rome icllr 2017
Rome  icllr 2017Rome  icllr 2017
Rome icllr 2017
 
Rome ichlsr
Rome  ichlsr Rome  ichlsr
Rome ichlsr
 
Rome icbmls 2017
Rome icbmls 2017Rome icbmls 2017
Rome icbmls 2017
 
Wb june ictel
Wb june ictelWb june ictel
Wb june ictel
 
Wb june icrst
Wb june icrstWb june icrst
Wb june icrst
 

Recently uploaded

practical guide of obesityfinal hyper1د حاتم البيطار القمة اكاديمي 0120238902...
practical guide of obesityfinal hyper1د حاتم البيطار القمة اكاديمي 0120238902...practical guide of obesityfinal hyper1د حاتم البيطار القمة اكاديمي 0120238902...
practical guide of obesityfinal hyper1د حاتم البيطار القمة اكاديمي 0120238902...
د حاتم البيطار
 
The Power of Superfoods and Exercise.pdf
The Power of Superfoods and Exercise.pdfThe Power of Superfoods and Exercise.pdf
The Power of Superfoods and Exercise.pdf
Dr Rachana Gujar
 
DECODING THE RISKS - ALCOHOL, TOBACCO & DRUGS.pdf
DECODING THE RISKS - ALCOHOL, TOBACCO & DRUGS.pdfDECODING THE RISKS - ALCOHOL, TOBACCO & DRUGS.pdf
DECODING THE RISKS - ALCOHOL, TOBACCO & DRUGS.pdf
Dr Rachana Gujar
 
Exploring the Benefits of Binaural Hearing: Why Two Hearing Aids Are Better T...
Exploring the Benefits of Binaural Hearing: Why Two Hearing Aids Are Better T...Exploring the Benefits of Binaural Hearing: Why Two Hearing Aids Are Better T...
Exploring the Benefits of Binaural Hearing: Why Two Hearing Aids Are Better T...
Ear Solutions (ESPL)
 
Let's Talk About It: Breast Cancer (What is Mindset and Does it Really Matter?)
Let's Talk About It: Breast Cancer (What is Mindset and Does it Really Matter?)Let's Talk About It: Breast Cancer (What is Mindset and Does it Really Matter?)
Let's Talk About It: Breast Cancer (What is Mindset and Does it Really Matter?)
bkling
 
Top massage center in ajman chandrima Spa
Top massage center in ajman chandrima  SpaTop massage center in ajman chandrima  Spa
Top massage center in ajman chandrima Spa
Chandrima Spa Ajman
 
Get Covid Testing at Fit to Fly PCR Test
Get Covid Testing at Fit to Fly PCR TestGet Covid Testing at Fit to Fly PCR Test
Get Covid Testing at Fit to Fly PCR Test
NX Healthcare
 
GIT BS.pptx about human body their structure and
GIT BS.pptx about human body their structure andGIT BS.pptx about human body their structure and
GIT BS.pptx about human body their structure and
MuzafarBohio
 
HUMAN BRAIN.pptx.PRIYA BHOJWANI@GAMIL.COM
HUMAN BRAIN.pptx.PRIYA BHOJWANI@GAMIL.COMHUMAN BRAIN.pptx.PRIYA BHOJWANI@GAMIL.COM
HUMAN BRAIN.pptx.PRIYA BHOJWANI@GAMIL.COM
priyabhojwani1200
 
COPD Treatment in Ghatkopar,Mumbai. Dr Kumar Doshi
COPD Treatment in Ghatkopar,Mumbai. Dr Kumar DoshiCOPD Treatment in Ghatkopar,Mumbai. Dr Kumar Doshi
COPD Treatment in Ghatkopar,Mumbai. Dr Kumar Doshi
Dr Kumar Doshi
 
Professional Secrecy: Forensic Medicine Lecture
Professional Secrecy: Forensic Medicine LectureProfessional Secrecy: Forensic Medicine Lecture
Professional Secrecy: Forensic Medicine Lecture
DIVYANSHU740006
 
一比一原版(EUR毕业证)鹿特丹伊拉斯姆斯大学毕业证如何办理
一比一原版(EUR毕业证)鹿特丹伊拉斯姆斯大学毕业证如何办理一比一原版(EUR毕业证)鹿特丹伊拉斯姆斯大学毕业证如何办理
一比一原版(EUR毕业证)鹿特丹伊拉斯姆斯大学毕业证如何办理
gjsma0ep
 
National Rural Health Mission(NRHM).pptx
National Rural Health Mission(NRHM).pptxNational Rural Health Mission(NRHM).pptx
National Rural Health Mission(NRHM).pptx
Jyoti Chand
 
2024 HIPAA Compliance Training Guide to the Compliance Officers
2024 HIPAA Compliance Training Guide to the Compliance Officers2024 HIPAA Compliance Training Guide to the Compliance Officers
2024 HIPAA Compliance Training Guide to the Compliance Officers
Conference Panel
 
Hypotension and role of physiotherapy in it
Hypotension and role of physiotherapy in itHypotension and role of physiotherapy in it
Hypotension and role of physiotherapy in it
Vishal kr Thakur
 
Champions of Health Spotlight On Leaders Shaping Germany's Healthcare.pdf
Champions of Health Spotlight On Leaders Shaping Germany's Healthcare.pdfChampions of Health Spotlight On Leaders Shaping Germany's Healthcare.pdf
Champions of Health Spotlight On Leaders Shaping Germany's Healthcare.pdf
eurohealthleaders
 
Know Latest Hiranandani Hospital Powai News.pdf
Know Latest Hiranandani Hospital Powai News.pdfKnow Latest Hiranandani Hospital Powai News.pdf
Know Latest Hiranandani Hospital Powai News.pdf
Dr. Sujit Chatterjee CEO Hiranandani Hospital
 
R3 Stem Cell Therapy: A New Hope for Women with Ovarian Failure
R3 Stem Cell Therapy: A New Hope for Women with Ovarian FailureR3 Stem Cell Therapy: A New Hope for Women with Ovarian Failure
R3 Stem Cell Therapy: A New Hope for Women with Ovarian Failure
R3 Stem Cell
 
Vicarious movements or trick movements_AB.pdf
Vicarious movements or trick movements_AB.pdfVicarious movements or trick movements_AB.pdf
Vicarious movements or trick movements_AB.pdf
Arunima620542
 
MBC Support Group for Black Women – Insights in Genetic Testing.pdf
MBC Support Group for Black Women – Insights in Genetic Testing.pdfMBC Support Group for Black Women – Insights in Genetic Testing.pdf
MBC Support Group for Black Women – Insights in Genetic Testing.pdf
bkling
 

Recently uploaded (20)

practical guide of obesityfinal hyper1د حاتم البيطار القمة اكاديمي 0120238902...
practical guide of obesityfinal hyper1د حاتم البيطار القمة اكاديمي 0120238902...practical guide of obesityfinal hyper1د حاتم البيطار القمة اكاديمي 0120238902...
practical guide of obesityfinal hyper1د حاتم البيطار القمة اكاديمي 0120238902...
 
The Power of Superfoods and Exercise.pdf
The Power of Superfoods and Exercise.pdfThe Power of Superfoods and Exercise.pdf
The Power of Superfoods and Exercise.pdf
 
DECODING THE RISKS - ALCOHOL, TOBACCO & DRUGS.pdf
DECODING THE RISKS - ALCOHOL, TOBACCO & DRUGS.pdfDECODING THE RISKS - ALCOHOL, TOBACCO & DRUGS.pdf
DECODING THE RISKS - ALCOHOL, TOBACCO & DRUGS.pdf
 
Exploring the Benefits of Binaural Hearing: Why Two Hearing Aids Are Better T...
Exploring the Benefits of Binaural Hearing: Why Two Hearing Aids Are Better T...Exploring the Benefits of Binaural Hearing: Why Two Hearing Aids Are Better T...
Exploring the Benefits of Binaural Hearing: Why Two Hearing Aids Are Better T...
 
Let's Talk About It: Breast Cancer (What is Mindset and Does it Really Matter?)
Let's Talk About It: Breast Cancer (What is Mindset and Does it Really Matter?)Let's Talk About It: Breast Cancer (What is Mindset and Does it Really Matter?)
Let's Talk About It: Breast Cancer (What is Mindset and Does it Really Matter?)
 
Top massage center in ajman chandrima Spa
Top massage center in ajman chandrima  SpaTop massage center in ajman chandrima  Spa
Top massage center in ajman chandrima Spa
 
Get Covid Testing at Fit to Fly PCR Test
Get Covid Testing at Fit to Fly PCR TestGet Covid Testing at Fit to Fly PCR Test
Get Covid Testing at Fit to Fly PCR Test
 
GIT BS.pptx about human body their structure and
GIT BS.pptx about human body their structure andGIT BS.pptx about human body their structure and
GIT BS.pptx about human body their structure and
 
HUMAN BRAIN.pptx.PRIYA BHOJWANI@GAMIL.COM
HUMAN BRAIN.pptx.PRIYA BHOJWANI@GAMIL.COMHUMAN BRAIN.pptx.PRIYA BHOJWANI@GAMIL.COM
HUMAN BRAIN.pptx.PRIYA BHOJWANI@GAMIL.COM
 
COPD Treatment in Ghatkopar,Mumbai. Dr Kumar Doshi
COPD Treatment in Ghatkopar,Mumbai. Dr Kumar DoshiCOPD Treatment in Ghatkopar,Mumbai. Dr Kumar Doshi
COPD Treatment in Ghatkopar,Mumbai. Dr Kumar Doshi
 
Professional Secrecy: Forensic Medicine Lecture
Professional Secrecy: Forensic Medicine LectureProfessional Secrecy: Forensic Medicine Lecture
Professional Secrecy: Forensic Medicine Lecture
 
一比一原版(EUR毕业证)鹿特丹伊拉斯姆斯大学毕业证如何办理
一比一原版(EUR毕业证)鹿特丹伊拉斯姆斯大学毕业证如何办理一比一原版(EUR毕业证)鹿特丹伊拉斯姆斯大学毕业证如何办理
一比一原版(EUR毕业证)鹿特丹伊拉斯姆斯大学毕业证如何办理
 
National Rural Health Mission(NRHM).pptx
National Rural Health Mission(NRHM).pptxNational Rural Health Mission(NRHM).pptx
National Rural Health Mission(NRHM).pptx
 
2024 HIPAA Compliance Training Guide to the Compliance Officers
2024 HIPAA Compliance Training Guide to the Compliance Officers2024 HIPAA Compliance Training Guide to the Compliance Officers
2024 HIPAA Compliance Training Guide to the Compliance Officers
 
Hypotension and role of physiotherapy in it
Hypotension and role of physiotherapy in itHypotension and role of physiotherapy in it
Hypotension and role of physiotherapy in it
 
Champions of Health Spotlight On Leaders Shaping Germany's Healthcare.pdf
Champions of Health Spotlight On Leaders Shaping Germany's Healthcare.pdfChampions of Health Spotlight On Leaders Shaping Germany's Healthcare.pdf
Champions of Health Spotlight On Leaders Shaping Germany's Healthcare.pdf
 
Know Latest Hiranandani Hospital Powai News.pdf
Know Latest Hiranandani Hospital Powai News.pdfKnow Latest Hiranandani Hospital Powai News.pdf
Know Latest Hiranandani Hospital Powai News.pdf
 
R3 Stem Cell Therapy: A New Hope for Women with Ovarian Failure
R3 Stem Cell Therapy: A New Hope for Women with Ovarian FailureR3 Stem Cell Therapy: A New Hope for Women with Ovarian Failure
R3 Stem Cell Therapy: A New Hope for Women with Ovarian Failure
 
Vicarious movements or trick movements_AB.pdf
Vicarious movements or trick movements_AB.pdfVicarious movements or trick movements_AB.pdf
Vicarious movements or trick movements_AB.pdf
 
MBC Support Group for Black Women – Insights in Genetic Testing.pdf
MBC Support Group for Black Women – Insights in Genetic Testing.pdfMBC Support Group for Black Women – Insights in Genetic Testing.pdf
MBC Support Group for Black Women – Insights in Genetic Testing.pdf
 

Grds international conference on health and life (8)

  • 1. Januar Natanael, Rizkita Rachmi Esyanti,Robert Manurung Department of Biological Engineering School of Life Sciences and Technology Institut Teknologi Bandung, Indonesia
  • 2.   Eurycoma longifolia Jack or tongkat ali can be used as a medicine to cure malaria, anti-inflammatory, anti- microbial, aphrodisiac and is still being developed as anticancer (Bhat, et al., 2010) Problem:  excess exploitation of this plants  critically endangered (Sudiarto, et al., 2002) Alternative method  tissue culture technique Introduction
  • 3.   Elicitation is a treatment given to culture, such as by exposing ultra-violet light, to induce the production of specific metabolites  UV elicitation has been proved able to induce the formation of the enzyme tryptophan decarboxylase on Catharantus roseus L.  alkaloid production increased (Ouwerkerk et al., 1999) Introduction
  • 4.   Biomass propagation in the bioreactor is a way to increase the biomass and secondary metabolites production in a larger scale.  There are several factors that must be considered in the scale-up process, such as: profiles of stirring, the solubility of oxygen in the medium, the exact dimensions of the reactor, the size of the cells used, and many more (Taticek et al., 1991) Introduction
  • 5.   In this study, the effect of scale-up of cell suspension cultures of E. longifolia in bioreactor conditions and treated by UV light on the growth and secondary metabolite production will be evaluated. Objective
  • 6.  Methods Subcultivated callus in fresh solid media Initiation cell suspension from callus Next MS Medium half strength 1,1 ppm 2,4-D and 1 ppm kinetin MS Solid Medium half stregth 2,2 ppm 2,4-D and 2 ppm kinetin
  • 7.  Methods Subcultured cell aggregate into fresh liquid and bioreactor for treatment Analysis Treatment: Exposed by UV 18 hr/day Rotation: 100 rpm Air flow rate: 0.3 vvm
  • 8.  E. longifolia Cell Culture Specific Growth Rate y = 0.0257x + 0.0468 R² = 0.9129 y = 0.0225x - 0.019 R² = 0.9316 y = 0.0549x -0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 0 2 4 6 8 10 12 14 ln(Xt/Xo) Day Flask-Control Flask-UV Bioreactor-UV * * Ouwerkerk et al., 1999 dan Stephanopoulus et al., 1998). ** Shuler dan Kargi, 2002 dan Schlatmann et al., 1994 **
  • 9.  Medium Conductivity Profile of E. longifolia Cell Culture 2 2.5 3 3.5 4 4.5 5 5.5 6 0 2 4 6 8 10 12 14 Conductivity(mS) Day Flask-Control Flask-UV Bioreactor-UV *Taya et al., 1989 dan Hahlbrock, 1975
  • 10.  Sucrose Consumption of E. longifolia Cell Culture y = -0.56x + 24.86 R² = 0.99145 y = -0.43x + 25.34 R² = 0.91009 y = -0.18x + 25 13.00 15.00 17.00 19.00 21.00 23.00 25.00 27.00 -1 4 9 14 SucroseLevel(g/L) Day Flask-Control Flask-UV Bioreactor-UV * Shuler dan Kargi, 2002 ** Shuler dan Kargi, 2002 ** *
  • 11.  Secondary Metabolites of E. longifolia Cell Culture Day Metabolites in Control Culture Amount Day Metabolites in UV-Exposed Culture Amount Day Metabolites in UV- Exposed Bioreactor Amount 9 Eurylactone 3.75% 4 Squalene 4.38% 14 Canthin-6-one 6.81% 12 5-(hydroxymethyl)-2- Furancarboxaldehyde 20.52% 9 Squalene 8.04% 14 Beta-carboline 29.24% 14 Canthin-6-one 2.67% 12 Quasinoid 4.03% 14 Squalene 3.50% 14 Canthin-6-one 5.12% 14 Beta-carboline 25.11% * Arvy et al., 1994; Fett-Neto dan Zhang, 1994; dan Ouwerkerk et al., 1999
  • 12.   E. longifolia cell cultures that were grown in bioreactor had a faster growth rate than in the flask culture.  UV light inhibits the growth of E. longifolia cell culture  The faster the rate of biomass formation, the faster the rate of sucrose and mineral salts consumption.  Elicitation with UV light increases the production of secondary metabolites in both the flask scale culture and bioreactor scale culture. Conclusions