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Enhancement of Lycopene and β-carotene Production in
Cherry Tomato Fruits (Solanum lycopersicum L. var.
cerasiforme) by Using Red and Blue Light Treatment
Calvin Lo, Robert Manurung, Rizkita Rachmi Esyanti
School of Life Sciences and Technology
Institut Teknologi Bandung, Indonesia
calvinloe.92@gmail.com, manurung@sith.itb.ac.id, rizkita@sith.itb.ac.id
Introduction
• Lycopene  prevention of cancer, cardiovascular disorder, etc.
(Kong et al., 2010)
• β-carotene  provitamin A & natural pigment.
• Prospective high economic value
(Pizarro & Stange, 2009)
(Kondo et al., 2014)
Aims of Study
• To determine the optimal light spectrum to enhance
lycopene, β-carotene and sugar production in cherry tomato
fruits.
• To construct mass distribution of lycopene, β-carotene and
sugar production in plant treated with red, white and blue
light exposure.
Methods
Vegetative Growth
2
2.5
3
3.5
4
4.5
0 7 14 21 28 35
ln(plantdryweight)
Period of treatment (days)
Control
Red
White
Blue
Treatment Specific growth rate (day-1) Doubling time (days)
Control 0.0572 12.2
Red 0.0642 10.8
White 0.0719 9.6
Blue 0.0677 10.2
Fruit Biomass Yield
0
100
200
300
400
500
600
700
Control Red White Blue
Totalmassofharvestedfruit
(g)
Treatment
Gilberto et al. (2005):
Overexpression Cry2 inhibited flowering time
Sugar Content (Brix Analysis)
1 °Brix ≅ 10%-w of sucrose
0
2
4
6
8
10
Control Red White Blue
Sugarcontent(°Brix)
Period of treatment (days)
Sugar 7 days
Sugar 28 days
Lycopene & β-carotene Content
0
50
100
150
200
Control Red White Blue
Lycopene&β-carotenecontent
(mg/kgfruit)
Treatments
Lycopene 7 days
Lycopene 28 days
Beta-carotene 7 days
Beta-carotene 28 days
Mass Distribution
Treatment
Vegetative
biomass (g)
Fruit biomass
(g)
Total amount produced in fruit
Lycopene (mg) β-carotene (mg) Sugar (g)
Control 41.33 47.7 63.78 17.46 41.25
Red 50.28 55.0 86.62 25.18 51.87
White 62.43 41.0 40.17 15.68 30.61
Blue 55.54 45.9 52.43 16.93 36.37
Conclusion
•Red light (663 nm) treatment enhanced lycopene, β-carotene and
sugar production in cherry tomato fruits, with increment of 35.8%,
46.5%, and 27.5% respectively.
•The best vegetative growth was observed on tomato plants treated
with white light, while the highest yield of fruit (1.15 fold), sugar,
lycopene and β-carotene were observed on plants treated with red
light.
Thank You

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Grds international conference on health and life (4)

  • 1. Enhancement of Lycopene and β-carotene Production in Cherry Tomato Fruits (Solanum lycopersicum L. var. cerasiforme) by Using Red and Blue Light Treatment Calvin Lo, Robert Manurung, Rizkita Rachmi Esyanti School of Life Sciences and Technology Institut Teknologi Bandung, Indonesia calvinloe.92@gmail.com, manurung@sith.itb.ac.id, rizkita@sith.itb.ac.id
  • 2. Introduction • Lycopene  prevention of cancer, cardiovascular disorder, etc. (Kong et al., 2010) • β-carotene  provitamin A & natural pigment. • Prospective high economic value
  • 3. (Pizarro & Stange, 2009) (Kondo et al., 2014)
  • 4. Aims of Study • To determine the optimal light spectrum to enhance lycopene, β-carotene and sugar production in cherry tomato fruits. • To construct mass distribution of lycopene, β-carotene and sugar production in plant treated with red, white and blue light exposure.
  • 6. Vegetative Growth 2 2.5 3 3.5 4 4.5 0 7 14 21 28 35 ln(plantdryweight) Period of treatment (days) Control Red White Blue Treatment Specific growth rate (day-1) Doubling time (days) Control 0.0572 12.2 Red 0.0642 10.8 White 0.0719 9.6 Blue 0.0677 10.2
  • 7. Fruit Biomass Yield 0 100 200 300 400 500 600 700 Control Red White Blue Totalmassofharvestedfruit (g) Treatment Gilberto et al. (2005): Overexpression Cry2 inhibited flowering time
  • 8. Sugar Content (Brix Analysis) 1 °Brix ≅ 10%-w of sucrose 0 2 4 6 8 10 Control Red White Blue Sugarcontent(°Brix) Period of treatment (days) Sugar 7 days Sugar 28 days
  • 9. Lycopene & β-carotene Content 0 50 100 150 200 Control Red White Blue Lycopene&β-carotenecontent (mg/kgfruit) Treatments Lycopene 7 days Lycopene 28 days Beta-carotene 7 days Beta-carotene 28 days
  • 10. Mass Distribution Treatment Vegetative biomass (g) Fruit biomass (g) Total amount produced in fruit Lycopene (mg) β-carotene (mg) Sugar (g) Control 41.33 47.7 63.78 17.46 41.25 Red 50.28 55.0 86.62 25.18 51.87 White 62.43 41.0 40.17 15.68 30.61 Blue 55.54 45.9 52.43 16.93 36.37
  • 11. Conclusion •Red light (663 nm) treatment enhanced lycopene, β-carotene and sugar production in cherry tomato fruits, with increment of 35.8%, 46.5%, and 27.5% respectively. •The best vegetative growth was observed on tomato plants treated with white light, while the highest yield of fruit (1.15 fold), sugar, lycopene and β-carotene were observed on plants treated with red light.