Cellular respiration involves three main stages: 1. Glycolysis produces 2 ATP and 2 NADH per glucose molecule. 2. The Krebs cycle produces 8 NADH, 2 FADH2, and 2 GTP per glucose. 3. Electron transport chain uses the NADH and FADH2 to produce 34 more ATP per glucose. Overall, cellular respiration results in the production of 36 ATP from one glucose molecule.

1. Cellular Respiration C 6 H 12 O 6 + 6O 2 6CO 2 + 6H 2 O + 36 ATP

2. 1. Glycolysis

3. 1. Glycolysis TOTALS _____ ATP produced _____ ATP used _____ ATP net gain _____ NADH produced 2 C 6 H 12 O 6 C C C = O H O H H H H O P i C C C = O H O H H H H O P i P-Gal C C C = O O - O H H H H H Pyruvic Acid C C C = O O - O H H H H H Pyruvic Acid ADP + P i ATP ADP + P i ATP NAD + NADH NAD + NADH ATP ATP ADP + P i ADP + P i ATP ATP ADP + P i ADP + P i Investment Phase Two ATP’s are hydrolyzed 4 2 2

4. 2. Krebs Cycle

5. Pyruvic Acid (3C) Acetic Acid (2C) 5C C-C-C-C- C 4C C-C-C-C 4C 4C Oxaloacetate (4C) 4C Krebs Cycle TOTALS (per molecule of glucose) _____ NADH produced _____ FADH 2 produced _____ GTP produced _____ CO2 produced NOT Citric Acid (6C) Citric Acid (6C) C-C-C-C- C-C CO 2 CO 2 CO 2 NAD + NADH NAD + NADH NAD + NADH GTP GDP + P i FADH 2 FAD + NAD + NADH 8 2 2 6

6. 3. Electron Transport Chain

7. I II III IV NADH H + H + H + H + H + H + H + H + H + H + H + H + H + H + ATP Synthase Inter- Membrane Space Matrix Inner membrane NAD + e - e - e - e - Enzyme spins ADP P i ATP 34 ATP from 1 glucose

8. For every NADH in the ETC, 3 ATP’s are produced For every FADH 2 in the ETC, 2 ATP’s are produced Cellular Respiration TOTALS ____ NADH in ETC ____ FADH 2 in ETC 10 2 30 4 34 4 2 36 38 ____ ATP produced ____ ATP produced ____ ATP produced in ETC ____ ATP produced in glycolysis ____ Subtotal ____ ATP used in glycolysis ____ ATP produced from one glucose in cellular respiration

9. Practice Slides

10. P-Gal C C C = O O - O H H H H H Pyruvic Acid Investment Phase 1. Glycolysis TOTALS _____ ATP produced _____ ATP used _____ ATP net gain _____ NADH produced C 6 H 12 O 6 C C C = O H O H H H H O P i C C C = O H O H H H H O P i C C C = O O - O H H H H H Pyruvic Acid

11. Pyruvic Acid (3C) Acetic Acid (2C) Citric Acid (6C) C-C-C-C- C-C NOT Citric Acid (6C) 5C C-C-C-C- C 4C C-C-C-C 4C 4C Ocaloacetate (4C) 4C Krebs Cycle TOTALS (per molecule of glucose) _____ NADH produced _____ FADH 2 produced _____ GTP produced _____ CO2 produced

12. I II III IV NADH H + H + H + H + H + H + H + H + H + H + H + H + H + H + ATP Synthase ADP P i