This is Part 3 of a 3-part series on the quest by scientists to find effective ways to fight skin aging. In our previous article, entitled “What have scientists found to fight the leading cause of skin aging – mitochondrial decay?”, we discussed how Schisandrin B and (−)Schisandrin B have been proven to be successful in that regard, dramatically improving the appearance of aging skin. A study related to this was recently published in the Journal of Cosmetic Dermatology, the official publication of the International Academy of Cosmetic Dermatology. “Mitochondria: a new focus as an anti-aging target in skin care”1 confirms the important role of mitochondrial function in aging and advocates targeting mitochondria in the development of new products. Here, we continue to explore the use of Schisandrin B and (−)Schisandrin B in the revolutionary new ingredient, Glissandrin™, which promises to change the way we approach skin care.

1. Glissandrin™: The Ultimate
Anti-Aging Skincare Ingredient
November 2011
Introduction
This is Part 3 of a 3-part series on the quest by scientists to find effective ways to fight
skin aging. In our previous article, entitled “What have scientists found to fight the
leading cause of skin aging – mitochondrial decay?”, we discussed how Schisandrin B
and (−)Schisandrin B have been proven to be successful in that regard, dramatically
improving the appearance of aging skin.
A study related to this was recently published in the Journal of Cosmetic Dermatology,
the official publication of the International Academy of Cosmetic Dermatology.
“Mitochondria: a new focus as an anti-aging target in skin care” 1 confirms the
important role of mitochondrial function in aging and advocates targeting
mitochondria in the development of new products.
Here, we continue to explore the use of Schisandrin B and (−)Schisandrin B in the
revolutionary new ingredient, Glissandrin™, which promises to change the way we
approach skin care.
As discussed in previous articles, mitochondrial decay plays a fundamental role in the
process of aging. Over time, cells lose the ability to function normally, falling victim to
free radicals and the ensuing oxidative stress. The consequences of this appear as the
visible signs of skin aging, including wrinkles, fine lines, age spots, loss of suppleness,
and a marked deterioration in skin tone.
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2. Glissandrin™
Glissandrin™ is a suite of natural compounds isolated from the Schisandra berry.
Consisting mainly of Schisandrin B and (−)Schisandrin B, Glissandrin™ also comprises
(±)γ-Schisandrin, Schisandrin A, and Schisandrin C. Extensive research i on
Glissandrin™ has confirmed its anti-aging properties on skin cells, most notably on its
ability to target the fundamental cause of aging: mitochondrial decay.
Research Findings
A. Mitochondrial decay in skin cells can be reversed by topical application of
Glissandrin™
A 3-day application of Glissandrin™ cream (2% and 5%) to skin cells caused a
dose-dependent increase in reduced glutathione (GSH) and α-tocopherol (α-Toc)
levels in skin mitochondria. Glissandrin™ cream is able to enhance the antioxidant
capacity of mitochondria and to reverse mitochondrial decay in aging skin cells.
Figure 1: Glissandrin™ enhances mitochondrial antioxidant capacity in the skin
Cream containing 2% or 5% Glissandrin™ was applied to skin cells on a daily basis for 3 days. The
control group was treated with cream containing no Glissandrin™. Skin tissues were isolated and
mitochondrial fractions were prepared. Mitochondrial reduced glutathione and α-tocopherol levels
were measured. P < 0.05 when compared to the control, using Student’s t-test.
i
GlissandrinTM is the result of over 20 years of research and development at the Hong Kong University of
Science and Technology (HKUST), led by Dr. Robert Ko, Professor of Life Science. HKUST was ranked
41st worldwide among research universities by Times Higher Education in 2010.
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3. B. Mitochondrial functional capabilities in skin cells can be enhanced by topical
application of Glissandrin™
Glissandrin™ also dose-dependently increased mitochondrial ATP (cellular
energy) production in human skin cells, indicative of enhancement in mitochondrial
functional ability.
Figure 2: Glissandrin™ enhances the mitochondrial functional ability in human skin cells
Cultured human skin cells were incubated with Glissandrin™ at the indicated concentration for 24
or 48 hours. ATP generation capacity was measured in situ and data were expressed as the
percentage of control. P < 0.05 when compared to the control, using Student’s t-test.
C. In-vivo and in-vitro studies have confirmed other properties of Glissandrin™
that are beneficial to the skin2:
• Emolliating proliferative skin conditions2
• Enhancing the natural antioxidative capabilities in skin cells3
• Increasing the expression of cellular heat shock proteins, thereby alerting
the skin to adverse external environmental factors3 4
• Suppressing inflammation5
• Inhibiting the production of collagenase, an enzyme that destroys collagen
• Protecting skin cells from solar radiation – particularly UV – and repairing
skin cells with UV damage5 6
• Reducing the destruction of elastin, a protein in the skin matrix
responsible for skin elasticity6
• Inhibiting ATR protein kinase activity (cancer prevention)7
Glissandrin™ has been clinically tested to be safe for even the most sensitive skin,
especially for users who experience adverse reactions to most skincare products.
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4. The desired level of biological availability and the purity of chemical compositions of
Glissandrin™ are standardized through a proprietary process developed specifically for its
manufacturing. Furthermore, the concentrations of the constituent compounds have been
formulated for easy absorption, optimal efficacy, and a luxurious feel when applied to the
skin.
Conclusion
The International Academy of Cosmetic Dermatology has spoken1, and its message is
clear: targeting mitochondrial decay is now the goal of the anti-aging industry.
Without understanding and addressing mitochondrial decay, the leading cause of skin
aging, most skincare products are ill-equipped to provide the real, sustainable results
they promise. Fortunately, Glissandrin™ is available todayii as the ultimate anti-aging
skincare solutioniii.
This article has been contributed by Glissandra Skincare.
More information on Schisandrin B, (−)Schisandrin B, and mitochondrial decay can be
found on these independent websites:
• National Institutes of Health (http://www.nih.gov/)
• PubMed (http://www.ncbi.nlm.nih.gov/pubmed/)
• Natural Standard (http://www.naturalstandard.com/)
ii
Glissandrin™ formulations are available under a licensing agreement.
iii
Glissandrin™ formulations are covered by the following patents:
US Non-Provisional Patent # US 7,276,257 B2
US Non-Provisional Patent # US 7,396,544 B2
US Non-Provisional Patent Publication No. 2008/0167372 A1
Pending - PCT Patent Application No. PCT/CN 2006/001804, filed on 21 July 2006
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5. About Glissandra™ Skincare Inc.
Glissandra™ Skincare Inc. is a network marketing company (also known as multi-level
marketing, MLM, or direct sales) dedicated to providing effective anti-aging skincare
through its holistic approach to skin health. Glissandra’s comprehensive skincare system
is the result of over 20 years of research and development at the Hong Kong University
of Science and Technology, led by Dr. Robert Ko. Over 100 research papers have been
published on its key proprietary ingredient, Glissandrin™, a suite of natural compounds
extracted from the Schisandra berry. In-vivo and in-vitro studies have proven the ability
of Glissandrin™ to address mitochondrial decay, the leading cause of aging, and to
enhance the cell’s natural ability to fight oxidative damage. (http://glissandra.com).
Contact Us
http://www.glissandra.com/
info@glissandra.com
1-877-313-7242
1
“Mitochondria: a new focus as an anti-aging target in skin care”; Menon et al, Global
R&D, ISP Corporation, New Jersey, USA; Journal of Cosmetic Dermatology 2010,
Wiley Periodicals Inc.
2
“Composition and Biological Activity of Different Extract from Schisandra
sphenanthera and Schisandra chinensis”; Huyke, et al., Department of Dermatology,
University Medical Centre, Freiburg, Germany; Planta Medica 2007.
3
“Schisandrin B-induced increase in cellular glutathione level and protection against
oxidant injury are mediated by the enhancement of glutathione synthesis and regeneration
in AML12 and H9c2 cells”, Ko et al., Biofactors 2006; 26: 221-230.
4
“Hepatoprotective mechanism of Schisandrin B: Role of mitochondrial glutathione
antioxidant status and heat shock proteins”, Ko et al., Free Radic. Biol. Med. 2003; 35:
368-380.
5
“Composition and Biological Activity of Different Extracts from Schisandra
sphenanthera and Schisandra chinensis”, Huyke et al., Department of Dermatology,
University Medical Center, Freiberg, Germany, Planta Med 2007 73: 1116-1126.
6
“Schisandrin B protects against solar irradiation-induced oxidative injury in BJ human
fibroblasts”; Ko et al.; Department of Biochemistry, the Hong Kong University of
Science and Technology; Fitoterapia 82 (2011) 682-691.
7
“Inhibition of ATR protein kinase activity by Schisandrin B in DNA damage response”,
H. Nishida et al., Department of Applied Life Science, Niigata University of Pharmacy
and Applied Life Sciences; Nucleic Acids Research 2009, Vol. 37, No. 17.
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