3. FDA DISCLAIMER
THIS MEDICAL AND SCIENTIFIC INFORMATION IS NOT FOR USE CONSUMERS.
THE DIETARY SUPPLEMENT PRODUCTS AND MEDICAL DEVICES OFFERED BY
CELVITALI MEDICAL HAVE NOT BEEN EVALUATED BY THE FDA AND ARE NOT
INTENDED FOR USE BY CONSUMERS OR MEDICAL PROVIDERS FOR BILLING
PURPOSES NOR AS A MEANS TO CURE, TREAT, PREVENT, DIAGNOSE, OR
MITIGATE ANY DISEASE OR OTHER MEDICAL CONDITION.
THIS IS NOT TO BE USED FOR SALES OR MARKETING. THIS IS FOR INTERNAL
EDUCATONAL PURPOSES ONLY.
4. •What are Growth Factors (GF)
• Basic skin anatomy and physiology
• How growth factors can help reverse the aging process
• Product line uses, benefits and applications
Overview
8. How Smoking Effects Skin
• Low level of collagen
production
• Nicotine and cadmium
suppress fibroblast
proliferation and hence
collagen production
• Hydrogen cyanide inhibits
oxygen delivery to cells in
dermal matrix – poor cell
function
Pre Post
9. How Smoking Effects Skin
Vasodilatation &
Inflammation
Fibroblast Proliferation &
Collagen Production
Collagen Cross-linking &
Matrix Maturation
Vasoconstriction
Nicotine
Inhibits Fibroblast Proliferation
And Function
Nicotine & Cadmium
Reduced Tissue Oxygenation
& Enzymatic Oxidative
Metabolism
Hydrogen Cyanide
10. What are Growth Factors
Growth factors are proteins that bind to receptors
on the cell membrane surface with the primary
function of activating cellular activities.
Many growth factors are quite versatile,
stimulating cellular processes in numerous
different cell types while others are specific to a
particular cell-type.
11. • Chemical messengers between cells
• Turn a variety of cellular activities “on” and “off”
• Can increase the rate at which cells in the body grow
•Proliferation
• Are involved with signaling new blood vessel growth
(angiogenesis) as well as collagen, elastin and fibronectin
production and distribution
•ECM – Extra Cellular Matrix (elasticity and resilience)
Functions of Growth Factors
12. • According to the US National Library of Medicine
and the National Center for Biotechnology
Information There are over 500 published scholarly
articles that discuss the topical application of growth
factors in regenerative medicine, wound healing and
anti-aging
• The use of skin based growth factors for aged skin
has been shown to positively counteract the aging
process which includes photo-damage, loss of
elasticity, uneven skin tone, and thinning of the
epidermis and dermis
Growth Factors in Medicine
13. How does Science define Growth
Factors & Cytokines?
• Growth factors are very specific peptides
(proteins) that emit biological signals
involved in the regulation of cell growth
and function.1,3
• For Example: TGF-b is a potent stimulator
of collagen production, promotes the
synthesis of extracellular matrix proteins
called glycosaminoglycan's (GAG’s), and
inhibits extra cellular matrix degradation
(thinning of your skin).1,2,3
• Cytokines are associated with
immune system cells and play a key
role in modulation of inflammation
• For example: PDGF (Platelet-derived
growth factors) and GM-CSF
(Granulocyte-Macrophage Colony-
Stimulating Factor), can stimulate
extracellular matrix production,
reduce inflammation, and promote
the formation of new blood vessels.1,3
1. Molecular Aspects of Dermatology. GC Priestley, ed. John Wiley & Sons, NY 1993
2. Arch Dermatology 138 (11): 1462-70, 2002
3. Growth Factors and Wound Healing. Ziegler, Pierce, Herndon, ed. Springer-Verlag NY 1997
14. Function of Cytokines and GF’s
Cytokine Function
TGF-beta Stimulates collagen, elastin and fibronectin production
(b1, b2, b3) Stimulates fibroblast collagen and glycosaminoglycan production
Increases fibronectin synthesis
Inhibits matrix degradation
Facilitates cell chemotaxis (healthy communication environment)
PDGF Stimulates fibroblast collagen and glycosaminoglycan production
Stimulates angiogenesis (new blood vessel growth)
Stimulates wound contraction
Recruits endogenous regenerative cells to region
GM-CSF Improves leukocyte function
Activates neutrophils, eosinophils, and monocytes/macrophages
Recruits endogenous regenerative cells to region
Interleukins Involved with Immunological Functions
(IL-3,IL-6, IL-7, IL-8, and IL-
16
Regulate hematopoietic and endothelial cell differentiation and proliferation
Involved with microenvironment homeostasis
Regulate inflammation
Immune cell response to pathogens
15. Aging of the Skin
• Aging of the skin can be regarded as a cellular process that is partially
genetically determined and partially influenced by environmental
factors (i.e. sun damage, air pollutants, etc.)
• The most obvious changes in the skin due to aging include: wrinkling,
laxity, dryness, and general thinning.
• Prominent wrinkling is mainly caused by the loss of dermal collagen
content, and a decreased rate of collagen production. 1 This is a result
of decreased growth factor production.
1. Molecular Aspects of Dermatology, GC Priestley, ed. John Wiley & Sons, NY 1993
2. Arch Dermatology 138 (11): 1462-70, 2002
3. Growth Factors and Wound Healing, Ziegler, Pierce, Herndon, ed. Springer-Verlag. NY 1997
16. The Fibroblast
• Dermal fibroblast cells are the "construction workers" of skin. A
decline in the number of fibroblast cells results in:
– less collagen production
– decreased concentration levels of growth factors
• Skin loses around 1% of the number of fibroblast cells and 1% of
its thickness per year.1
• The rate of decline in fibroblast cell number and function is
increased by sun exposure, recent studies have also shown that
sun damaged skin has 35% fewer blood capillaries than normal
skin3; thus receiving less nutrients and oxygen than normal
healthy skin.
1. Molecular Aspects of Dermatology, GC Priestley, ed. John Wiley & Sons, NY 1993
2. Arch Dermatology 138 (11): 1462-70, 2002
3. Growth Factors and Wound Healing, Ziegler, Pierce, Herndon, ed. Springer-Verlag. NY 1997
17. Growth Factors, Stem Cells and Plants…
Misconception vs. Fact
• Do Stem cells produce growth factors?
• Are they relevant?
• Why stem cell?
• Do growth factors from other species; e.g. plants
work on humans?
• What is the best source of growth factors?
18. Skin Growth Factors and Cytokines in
Summary
• Are very specific peptides (proteins)
• Involved in the communication process between cells and
tissues (lock and key)
• They regulate the cell’s growth, replication and
regeneration mechanisms (proliferation and
differentiation)
• Stimulate the skin’s own renewal mechanism
• Can increase hydration and promote elimination of toxins
(angiogenesis)
• Improve elasticity and resilience (ECM production)
• Aid in repairing the look of damaged skin
21. Manufacturing of Growth Factors
• Fibroblast cell line is screened , genetically modified, licensed and
patented
• Cells grow in enriched media for pure growth factor production
• Growth factors, cytokines, interleukins and other peptides are
produced by the cells
• Growth factors and peptides are harvested and purified from the
cell cultures then stored under stringent sterile conditions
• The products are formulated in a sterile environment in a FDA
registered facility under GMP to produce the product
Enrich cells for growth factor
production
22. • Are there any studies or published articles on negative
effects of topically applied growth factors?
•clear record of safety for 10+ years
•What is the assurance and theory behind safety?
•Growth Factors are naturally found in the body
• They act locally and don’t enter the blood stream
•Paracrine and not Endocrine (hormone)
•Paracrine refers to a hormone that has effect only in the
vicinity of the gland secreting it
•US FDA allows the use of GF in cosmetics
•No treatment claim
Safety: are Growth Factors safe for
topical application?
23. Impact of Quality on Results
What difference does it make?
o 94% of women saw improvement in overall appearance of
their skin
o 85% experienced improvement in skin texture/smoothness
o 79% felt increase in skin firmness and elasticity
o 87% saw reduction in the appearance of fine lines and
wrinkles
24. Alpha-Hydroxy Acids
• α-Hydroxy acids, or alpha hydroxy
acids (AHAs), are a class of chemical
compounds that consist of a carboxylic acid
substituted with a hydroxyl group on the
adjacent carbon.
• May well-known α-hydroxy acids are glycolic
acid, lactic acid, citric acid and mandelic acid.
25. Beta-hydroxy Acids
• A beta hydroxy acid or β-hydroxy acid (BHA)
is an organic compound contains a carboxylic
acid functional group and functional group
separated by two carbon atoms.
• Salicyclic Acid is a well-known beta-hydroxy
acid
26.
27. Restore Cleanser
Product Benefits:
All Skin Types
For all skin types, this gentle sulfate-free cleanser can be used daily to
help refine skin texture and fade discoloration and sun spots.
28. Restore Cleanser
Active Ingredients:
Aloe Vera - Improves the circulation and regenerates the cells. Its effects
are soothing, astringent, and protecting. Aloe is also excellent for
sensitive skins, rosacea conditions, and dry skin.
Citrus Aurantium Dulcis (Orange Peel) – An Anti-inflammatory and
antibacterial, it is valued for its invigorating, relaxing and lightening
abilities.
Olea Europaea (Olive Oil) – A carrier oil that helps stimulate the
synthesis of such substances as collagen, elastin, proteoglycans, and
glycoproteins over the skins surface.
Tocopheryl (vitamin E) – This wonderful vitamin, when applied topically,
is absorbed through the skin. It is attributed with reducing skin
pigmentation, decreasing functioning of the sebaceous glands, increasing
water retention in the skin, and serving as a super antioxidant and free
radical scavenger.
32. Revitalize Toner
Product Benefits:
An antiseptic toner that removes excess oil, dead cell build up and
flaky skin. It helps refine and unclog pores as it clarifies the
complexion. This formula contains Salicylic Acid, Witch Hazel
Extract, Lemon Peel Extract, Panthenol, Aloe Vera, Sodium
Ascorbate, Tocopheryl (Vit.E) and Retinol, (Vit.A).
Exfoliates and clarifies the complexion Dissolves excess oil
without over-drying Stimulates and improves circulation Tones
and balances skin
33. Revitalize Toner
Active Ingredients:
Salicylic Acid
This ingredient aids in dissolving the top layer of the stratum corneum by virtue of its
keratolytic nature, or exfoliation. Salicylic Acid is derived from the bark of sweet birch and the
leaves of wintergreen. It is also known for its antiseptic qualities and is acceptable for use on
most skin types, except those allergic to “aspirin” or extremely dry or extra sensitive skin.
Retinol Palmitate
A biologically active form of Vitamin A that can be converted to retinoic acid when applied to
the skin. Retinol’s benefits stems from its vital role in the
ability to produce new cell tissue, maintenance, and optimum cellular function.
Witch Hazel Extract
Known for its astringent properties. Witch Hazel extract is also toning, anti-inflammatory, and
regulating. Derived from the bark and leaves of the tree. It is a proficient vasoconstrictor effect
that is excellent for inflamed, irritated or acneic skin conditions. It is also used in ointments for
the treatment of sunburns, bruises and insect bites.
Panthenol
This highly moisturizing ingredient aids in tissue repair and stimulates cellular proliferation.
Panthenol also helps promote wound healing and is anti-inflammatory. Its ability to attract
water from the environment provides added hydration and suppleness to the skin.
37. Replenish Growth Factor
Product Benefits:
Formulated with patented growth factor technology, the face serum
includes concentrated proteins, growth factors and active ingredients,
that help revitalize the skin to create a firm, youthful looking
complexion. When regularly applied topically to the skin, this serum is
designed to naturally accelerate the skins renewal process by providing
the damaged skin cells with the necessary components of young,
healthy skin. These active ingredients aim to stimulate the production
of collagen in the deeper layers of the skin, resulting in improved
elasticity and overall appearance of the skin.
• Diminish deep lines and wrinkles
• Firm and tone the skin
• Improve the look of skin texture
• Create an even skin tone
• Fade sun damage
39. Replenish Growth Factor Serum
Usage:
• Carefully dispense a small amount of
serum onto clean fingers and gently
apply over the entire face, neck and
chest areas after cleansing and toning.
Repeat process twice daily, preferably
morning and night. Optimal results are
seen after 6 weeks of regular usage.
40.
41. Rejuvenate Daily Moisturizer
Ingredients:
Aloe Vera
Known for its anti‐inflammatory and antibiotic properties. Aloe Vera is made
up of the most precious substances of our own skin‐ enzymes, vitamins,
proteins, and minerals that support all skin functions and activate the skins
healing power. Aloe Vera helps maintain the skins moisture balance and
stimulates blood circulation. It has been used to calm and soothe burns and
irritations, and it is moisturizing.
Green Tea Leaf Extract (Camellia Sinensis)
This herbal extract is rich in potent antioxidants to protect skin from harmful
ultraviolet sunrays. It has astringent properties due to the tannic acid content
which helps reduce inflammation and swelling.
42. Spirulina Extract
Spirulina is a essential glyco-nutrient. It nourishes skin and contains essential
fatty acids and vitamins.
Tocopheryl Acetate (Vitamin E)
An antioxidant found in vegetable oils and other herbal essential oils. Also
known as Vitamin E, this wonderful vitamin, when applied topically, is easily
absorbed into the skin. It reduces unwanted skin pigmentation, decreases
production of sebaceous gland activity, and increases water retention in the
skin. It also serves as a super antioxidant and free radical scavenger. It also
helps protect from harmful UVA/UVB sunrays.
46. Repair Growth Factor Eye Serum
Product Benefits:
The Eye Serum is particularly intended to target the
delicate area around the eye. The concentrated proteins
of the advanced growth factor technology aim to tighten
and brighten the skin. The sensitive skin around the eye
will appear brighter and more refreshed with this
moisturizing formula that helps the broken capillaries
causing the dark circles, crow feet and fine lines.
• Lift and tighten skin along outer edges of the eye
• Smooth fine lines and sagging skin
• Reduce dark circles
• Gently moisturizes the skin around the eye
47. Dark Circles under eyes
• The most effective dark circles eye creams will have
advanced powerful ingredients like Hesperidin methyl
chalcone, Palmitoyl oligopeptide and Palmitoyl
tetrapeptide-3. Together these ingredients work to
diminish broken blood vessels under the skin which
cause dark under eye circles and stimulate collagen
production to repair flaws like fine lines and wrinkles.
• Hemosiderin is a pigment that is a byproduct of
hemoglobin in the blood that has spread into the tissues
following injury with bleeding and bruising of the tissues.
It contains iron pigment and may take along time (years)
for the body to metabolize the iron and pigment that has
spread into the tissues.
48. Hesperidin methyl chalcone
• Hesperidin is found in naturally high concentrations
in the peel, pulp, and membranes of citrus fruit,
such as grapefruits, lemons, and oranges.
Hesperidin methyl chalcone has antioxidant
properties, which help it to combat the
inflammation and cell damage caused by free
radicals.
• Hesperidin helps to reduce tiny blood vessels in the skin
around your eyes from breaking and leaking. This
leakage causes dark bruises or what you know as dark
circles.
49. Palmitoyl oligopeptide and Palmitoyl tetrapeptide-3
• Synthetically produced peptides comprised of four
amino acids that are to suppress the production of
excess interleukins, the chemical messengers which
trigger the body’s acute inflammatory response.
• These peptides work to stimulate collagen
production in the skin. Increased collagen
production helps diminish fine lines and wrinkles
around the eye area. Skin is younger and smoother
looking.
50. Retinyl palmitate
• This retinoid is a form of Vitamin A that works to
speed up the metabolism of the skin cells. It helps
regenerate new skin cell growth which leads to
radiant and rejuvenated skin.
51. Chrysin
• Chrysin is an naturally occurring isoflavone that
is extracted from plants, most often from
the passiflora caerulea (blue passion flower).
• Chrysin is an anti-oxidant and anti-inflammatory. It
works as a vasodiolator; which helps increase
proper blood flow around the eye. This helps
prevent blood vessels from breaking and bruising
and also enhances the effects of hesperidin.
53. Repair Growth Factor Eye Complex
Usage:
• Carefully dispense a small amount of serum onto
clean fingers. With gentle pressure, apply serum
using a circular motion around the entire eye area
until the serum is absorbed. Repeat process twice
daily, preferably morning and night. Optimal
results are seen after 6 weeks of regular usage.
54.
55.
56. Regenerate Night Cream
Product Benefits:
• This lush moisturizer is ideal for all skin types.
Enriched with Aloe and infused with Olive and
Jojoba oils, this moisturizer glides on for long-
lasting softness.
57. Regenerate Night Cream
Active Ingredients:
Aloe Vera - Improves the circulation and regenerates the cells. Its effects are
soothing, astringent, and protecting. Aloe is also excellent for sensitive skins,
rosacea conditions, and dry skin.
Olea Europaea (Olive Oil) – A carrier oil that helps stimulate the synthesis of
such substances as collagen, elastin, proteoglycans, and glycoproteins over
the skins surface.
Jojoba Oil – Beneficial to help treat dry or oily skin. If your skin has an over
production of sebum jojoba oil will dissolve clogged pores and restore the
skin to its natural pH balance. Helps reduces superficial lines, wrinkles and is
hypoallergenic and pure.
58. Sweet Almond Oil – An easily absorbable oil that is suitable for all skin
types, helps relieve irritation, inflammation and itching. Known for its
ability to soften and re-condition the skin. It is rich in proteins and
Vitamin D, and is considered extremely nourishing.
Tocopheryl (vitamin E) – This wonderful vitamin, when applied topically,
is absorbed through the skin. It is attributed with reducing skin
pigmentation, decreasing functioning of the sebaceous glands, increasing
water retention in the skin, and serving as a super antioxidant and free
radical scavenger.
62. FDA DISCLAIMER
THIS MEDICAL AND SCIENTIFIC INFORMATION IS NOT FOR USE CONSUMERS.
THE DIETARY SUPPLEMENT PRODUCTS AND MEDICAL DEVICES OFFERED BY
CELVITALI MEDICAL HAVE NOT BEEN EVALUATED BY THE FDA AND ARE NOT
INTENDED FOR USE BY CONSUMERS OR MEDICAL PROVIDERS FOR BILLING
PURPOSES NOR AS A MEANS TO CURE, TREAT, PREVENT, DIAGNOSE, OR
MITIGATE ANY DISEASE OR OTHER MEDICAL CONDITION.
THIS IS NOT TO BE USED FOR SALES OR MARKETING. THIS IS FOR INTERNAL
EDUCATONAL PURPOSES ONLY.
