Genetically Modified Organisms for the human welfare

1. GMO in somatotropin
production

2. The GMO
• A genetically modified organism (GMO) is
any organism whose genetic material has
been altered using genetic engineering
techniques

3. Somatotropin
• HGH
• It is a peptide hormone that stimulates
growth, cell reproduction, and cell
regeneration in humans and other animals.
• Growth hormone is produced in the growth-
stimulating somatotropic cells of the pituitary
gland, which is located at the base of the brain

4. Role in Humans
• stimulate the liver and other tissues to secrete
IGF-I. IGF-I (growth factors)
• stimulates proliferation of chondrocytes
(cartilage cells)
• Rapid growth of cells

5. Structure
(Primary and secondary )

6. Sequence
• 740 bp linear mRNA
• >NM_022560.3 Homo sapiens growth hormone 1 (GH1), mRNA
AAGAGACCAGCTCAAGGATCCCAAGGCCCAACTCCCCGAACCACTCAGGGTCCTGTGGACAGCTCACCTA
GCTGCAATGGCTACAGGCTCCCGGACGTCCCTGCTCCTGGCTTTTGGCCTGCTCTGCCTGCCCTGGCTTC
AAGAGGGCAGTGCCTTCCCAACCATTCCCTTATCCAGGCTTTTTGACAACGCTATGCTCCGCGCCCATCG
TCTGCACCAGCTGGCCTTTGACACCTACCAGGAGTTTAACCTAGAGCTGCTCCGCATCTCCCTGCTGCTC
ATCCAGTCGTGGCTGGAGCCCGTGCAGTTCCTCAGGAGTGTCTTCGCCAACAGCCTGGTGTACGGCGCCT
CTGACAGCAACGTCTATGACCTCCTAAAGGACCTAGAGGAAGGCATCCAAACGCTGATGGGGAGGCTGGA
AGATGGCAGCCCCCGGACTGGGCAGATCTTCAAGCAGACCTACAGCAAGTTCGACACAAACTCACACAAC
GATGACGCACTACTCAAGAACTACGGGCTGCTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACAT
TCCTGCGCATCGTGCAGTGCCGCTCTGTGGAGGGCAGCTGTGGCTTCTAGCTGCCCGGGTGGCATCCCTG
TGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATA
AAATTAAGTTGCATCATTTTGTCTGAAAAAAAAAAAAAAA

7. Production

8. The strategies
• Enzymes used: EcoRI; Pvu II; BamHI
• Vector used: recombinant expression vector -
PhGH
• Host used: Escherichia coli

10. Somatotropin supplement