This document summarizes the formulation, characterization, and in vitro evaluation of aceclofenac emulgel for topical application. Eight aceclofenac emulgel formulations were prepared using a factorial design with variables including gelling agent, light liquid paraffin, and emulsifying agent. The formulations were evaluated for properties such as appearance, pH, viscosity, spreadability, and drug content. In vitro drug permeation studies showed that formulation F7 had the highest permeation, with 89.97% cumulative drug release after 12 hours. Kinetic analysis indicated F7 followed Higuchi diffusion, indicating drug release was diffusion mediated. Thus, F7 was selected as the optimized aceclofenac emulgel formulation for
it describes the controlled drug release by diffusion or dissolution or both or swelling or erosion and which kinetics it follows either zero,first , higuchi or peppas
Optimization technique is a rational approach for selecting the excipients, their concentrations and process conditions for obtaining the best possible product satisfying the quality characteristics.
Optimization is an act, process or methodology of making design, system or decisions as fully perfect, functional or as effective as possible.
FORMULATION AND EVALUATION OF OCUSERTS OF CIPROFLOXACIN HClMohammad Adil
Conventional ocular drug delivery system i.e., eye drops, ointments, gels etc., had become less popular pertaining to their disadvantages like evaporation by tears, pre-corneal loss, drug metabolism, drug-protein interaction, drainage, sticking of eye lids, induced lacrimation, poor patient compliance, systemic side effect and blurred vision etc. That’s why fundamentals of controlled release by means of ocular inserts were utilized to increase problem pre-corneal drug residence time.
This project title “Formulation and Evaluation of Ocuserts of Ciprofloxacin HCl” revealed following results:
Compatibility study using FTIR was performed to check the compatibility of drug with various excipient. Characteristics peaks obtained with pure drug were compared with that produced with different excipients that confirmed the compatibility of drug with excipients.
Ocusert of Ciprofloxacin HCl was prepared using different material i.e., PVP K-30, PVA, PEG 400 and glycerin.
Prepared ocuserts were evaluated for various parameters viz., percentage moisture loss, percentage moisture absorbs, thickness, weight variation, drug content and In-vitro diffusion.
The percentage (%) moisture absorption and loss of ocular insert were found to be 26% and 27% respectively.
The thickness of ocular insert was found to be uniformed and its mean while measuring at different points was found to be 0.124mm.
The weight of ocular inserts was found to be in the range of 12.2 - 12.6mg which indicated decent distribution of the drug, polymer and plasticizer.
The drug content of ocular insert was found to be 99.89%.
Percentage drug release from Ciprofloxacin HCl Ocusert was found to be 41.969% in 8 hr.
It was concluded that prepared Ocusert of Ciprofloxacin HCl could be a better alternative to conventional ocular formulations that retained on ocular surface for longer duration and released drug in controlled manner.
it describes the controlled drug release by diffusion or dissolution or both or swelling or erosion and which kinetics it follows either zero,first , higuchi or peppas
Optimization technique is a rational approach for selecting the excipients, their concentrations and process conditions for obtaining the best possible product satisfying the quality characteristics.
Optimization is an act, process or methodology of making design, system or decisions as fully perfect, functional or as effective as possible.
FORMULATION AND EVALUATION OF OCUSERTS OF CIPROFLOXACIN HClMohammad Adil
Conventional ocular drug delivery system i.e., eye drops, ointments, gels etc., had become less popular pertaining to their disadvantages like evaporation by tears, pre-corneal loss, drug metabolism, drug-protein interaction, drainage, sticking of eye lids, induced lacrimation, poor patient compliance, systemic side effect and blurred vision etc. That’s why fundamentals of controlled release by means of ocular inserts were utilized to increase problem pre-corneal drug residence time.
This project title “Formulation and Evaluation of Ocuserts of Ciprofloxacin HCl” revealed following results:
Compatibility study using FTIR was performed to check the compatibility of drug with various excipient. Characteristics peaks obtained with pure drug were compared with that produced with different excipients that confirmed the compatibility of drug with excipients.
Ocusert of Ciprofloxacin HCl was prepared using different material i.e., PVP K-30, PVA, PEG 400 and glycerin.
Prepared ocuserts were evaluated for various parameters viz., percentage moisture loss, percentage moisture absorbs, thickness, weight variation, drug content and In-vitro diffusion.
The percentage (%) moisture absorption and loss of ocular insert were found to be 26% and 27% respectively.
The thickness of ocular insert was found to be uniformed and its mean while measuring at different points was found to be 0.124mm.
The weight of ocular inserts was found to be in the range of 12.2 - 12.6mg which indicated decent distribution of the drug, polymer and plasticizer.
The drug content of ocular insert was found to be 99.89%.
Percentage drug release from Ciprofloxacin HCl Ocusert was found to be 41.969% in 8 hr.
It was concluded that prepared Ocusert of Ciprofloxacin HCl could be a better alternative to conventional ocular formulations that retained on ocular surface for longer duration and released drug in controlled manner.
Dissolution, factors affecting drug dissolution, methods to evaluate dissolution, advantages and disadvantages, recent approaches--these are the topics covered in this presentation.
This slide share includes the introduction about smedds, difference between emulsion and smedd and sedds and smedds, composition and its formulation aspects.
Dissolution, factors affecting drug dissolution, methods to evaluate dissolution, advantages and disadvantages, recent approaches--these are the topics covered in this presentation.
This slide share includes the introduction about smedds, difference between emulsion and smedd and sedds and smedds, composition and its formulation aspects.
INTRODUCTION
Conventional oral drug delivery systems are known to provide an immediate release of drug, in which one cannot control the release of the drug and effective concentration at the target site. The bioavailability of drug from these formulations may vary significantly, depending on factors such as physico-chemical properties of the drug, presence of excipients, various physiological factors such as the presence or absence of food, pH of the GI tract, GI motility, etc. so to overcome this limitation oral route is replied by parenteral route. This route offers the advantage of reduced dose, targeting of site and avoiding GI stability, hepatic by-pass of drug molecule. (1)
In recent years, much attention has focused on novel drug delivery systems (NDDS). There are many designing options available to control or modify the drug release from a dosage form. Numerous technologies have been used to control the systemic delivery of drugs.
Based on the mechanism of the drug release can be classified as:
Diffusion controlled (matrix and reservoir type of systems)
Dissolution controlled (surface eroding, surface swelling type of systems)
Osmotic drug delivery
Multi particulate systems
Enteric coated (pH dependent systems)
One of the most interesting one is that employs osmotic pressure as an energy source for release of drugs.
The role of drug development is to take a therapeutically effective molecule with sub-optimal physicochemical and/or physiological properties and develop an optimized product that will still be therapeutically effective with additional benefits such as:
Sustained and consistent blood levels within the therapeutic window
Enhanced bioavailability
Reduced interpatient variability
Customized delivery profiles
Decreased dosing frequency
Improved patient compliance
Reduced side effects.
Osmotically controlled oral drug delivery systems (OCODDS) utilize osmotic pressure as the energy source for the controlled delivery of drugs. Drug release from these systems is independent of pH and hydrodynamic conditions of the gastro-intestinal tract (GIT) to a large extent and release characteristics can be easily adjusted by optimizing the parameters of the delivery system.
PRINCIPLE
The flow of solvent depends on SPM characteristics and different osmosis pressures between two sides of regions.
Osmosis pressure for concentrated solution of soluble solutes commonly used in controlled release formulation are extremely high, ranging from 30 atm for sodium phosphate up to 500 atm for a lactose-fructose mixture (US patent number 4077407). These osmosis pressures can produce high water flows across semi permeable membrane.
TYPES OF PUMPS
Oral Osmotic Pumps
Elementary osmotic pump(8)
Push Pull Osmotic Pump(9)
Controlled porosity Osmotic Pumps (CPOP)(10)
OROS CT System(11)
NEED OF THE STUDY
Present investigation is to develop controlled osmotic tablet of
Levetiracetam is an anti-epileptic medicine used to treat seizures in epilepsy. It is used for partial-onset, myoclonic, or tonic–clonic seizures. General route of administration is by Oral and IV. But it is not recommended throughout an epileptic attack due to the risk of nausea or vomiting. The Intranasal (IN) route is a promising therapy option, as it allows medications to reach the brain directly, it is regarded an alternative to parenteral administration, and hence the effective dosage predicted via other administration routes is scheduled to be reduced using the IN route
Abstract:
The Chronopharmacotherapy the drug administration is synchronised with circadian rhythms Formulation development of Microspheres is more reliable formulation as compare to single type dosage formulation due to it avoids dose dumping, as per required drug release profile is achieved For microspheres many polymers are used such as albumin, gelatine, starch, Eudragit, Polyacrylamide (“PAM”) these material loading capacity is high. Micro sponges which are Spherical are called as micro-balloons. Due to its hollow structure it shows good floating properties. In these systems use of Carbon-dioxide (CO2) as gas generating system which are used for floating purpose. The objective of present investigation is to prepared and evaluate a floating pulsatile drug delivery system of Aceclofenac. The strategy adopted for microspheres containing Aceclofenac as a material were prepared by emulsion solvent diffusion technique. Drug and polymer were mixed in dichloromethane and ethanol at 1:1 ratio. The drug and polymer solution were poured in water 50% W/V polyvinyl alcohol maintained at 30-40 C and the solution was stir at 500rpm using mechanical stirrer, The microspheres obtain were washed repeatedly with water until free from poly vinyl alcohol. The developed formulations were evaluated yield of floating microspheres particle size and shape, drug entrapment efficiency in-vitro evolution of floating ability, in-vitro drug release study. On the basis of these evolution parameters it was found that optimised floating pulsatile release formulation F7 showed higher drug entrapment efficiency floating time 6.8 minutes and the drug and polymer 32 1:3 ratio the particle size was increased.
Key Words: Chronopharmacotherapy, Floating pulsatile drug delivery, Aceclofenac.
“Intervention of a clinical pharmacist in order to reduce polypharmacy, avera...SriramNagarajan17
“Intervention of a clinical pharmacist in order to reduce polypharmacy, average cost of therapy and percentage of patients received injections (parenterals) in pediatrics dept; study carried out at multi-specialty teaching hospital”
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
Follow us on: Pinterest
Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Formulation, characterization and invitro evaluation of aceclofenacemulgel for topical application
1. Mahesh B et al / Journal of Pharmacreations Vol-3(1) 2016 [90-98]
90
Pharmacreations | Vol.3 | Issue 1 | Jan- Mar- 2016
Journal Home page: www.pharmacreations.com
Research article Open Access
Formulation, characterization and invitro evaluation of aceclofenacemulgel
for topical application
Mahesh BabuKondra*, SaradaPrasannaSethy
Sushrut Institute of Pharmacy, Taddanapally village, Pulkal madal, Medak, Sangareddy.
*Corresponding author: Mahesh BabuKondra
Email Id- m.m7773@gmail.com
ABSTRACT
Aceclofenac is categorized as anti-arthritic drug and is successfully used in the treatment of arthritis. Aceclofenac
maximum wavelength is determined by UV-Visible spectrophotometer using 6.8 pH phosphate buffer and was
detected to be 275 nm. Aceclofenac emulgel was formulated using light liquid paraffin as oil phase and emulsifying
agents tween 20 and span 20 for emulsion and incorporated into gel using CMC and carbopol 934 polymers in
different ratios. The optimized formulation F7 showed a shear thinning with thixotropic property with better
spreadability, viscosity and in-vitro permeability compared to other formulations. In the study it was observed that
the concentrations of tween20 spann 20 and light liquid paraffin has shown effect on viscosity, spreadability and in-
vitro drug permeability. Increased amount of liquid paraffin showed suppress activity of tween 20 and span 20.
Keywords: UV-Visible spectrophotometer, CMC and carbopol 934
INTRODUCTION
Transdermal drug delivery system has been in
existence for a long time. In the past, the most
commonly applied systems were topically applied
lotions, creams and ointments for dermatological
disorders4
. The occurrence of systemic side-effects
with some of these formulations is indicative of
absorption of the drugs through the skin, which lead
to the idea of TDDS. In a broad sense, the term
transdermal delivery system includes all topically
administered drug formulations intended to deliver
the active ingredient into the general circulation.
Transdermal therapeutic systems have been designed
to provide controlled continuous delivery of drugs via
the skin to the systemic circulation10
.
Transdermal drug delivery system (TDDS)
established itself as an integral part of novel drug
delivery systems2
. The novel Transdermal drug
delivery is defined as self-contained, discrete dosage
forms which when applied to the intact skin, deliver
the drug through the skin at controlled rate to the
systemic circulation1
.
OBJECTIVES
The main aim of present research work is:
Formulation of Aceclofenac Emulgel using
various emulsifying agents and gelling agents in
different combinations ratios by suitable method.
Evaluation of emulgel formulations for its physico-
chemical properties like visual appearance, viscosity,
pH, spreadability, drug content, etc.
In-vitro drug release permeation studies using Franz-
Diffusion cell7.
Journal of Pharmacreations
2. Mahesh B et al / Journal of Pharmacreations Vol-3(1) 2016 [90-98]
91
To predict the surface morphology SEM studies were
performed for the best formulation
METHODOLOGY
Identification and authenticity of Aceclofenac
pure drug
Physical appearance
Physical appearance of the drug was examined by
organoleptic properties, such as color, taste, odor and
state.
Determination of melting point
Melting point of the drug was determined by
taking small quantity of drug in a capillary tube
closed at one end which was then place in Tehsil’s
melting point apparatus. The temperature at which
the drug melts was noted using liquid paraffin as
liquid solvent. Average of triplicate readings was
recorded.
Formulation design for Aceclofenac emulgel
preparation:
The formulation code was designed according to
a 23
factorial design so total eight
Aceclofenacemulgel formulations were prepared. The
optimization in the formulation batches were made
mainly based on three factors i.e., gelling agent, light
liquid paraffin and emulsifying agent12
Formulation code for gel preparation (Aceclofenac 10% w/w) Table 1
Ingredients
(% w/w)
F1 F2 F3 F4 F5 F6 F7 F8
HPMC K15M 0.5 0.5 0.5 0.5 - - - -
Carbopol 934 - - - - 0.25 0.25 0.25 0.25
Purified water q.s q.s q.s q.s q.s q.s q.s q.s
Formulation code for emulsion preparation (Aceclofenac 10% w/w) Table 2
Ingredients
(% w/w)
F1 F2 F3 F4 F5 F6 F7 F8
Aceclofenac 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2
Light liquid paraffin 2.5 3.75 2.5 3.75 2.5 3.75 2.5 3.75
Tween 20 0.3 0.3 0.5 0.5 0.3 0.3 0.5 0.5
Span 20 0.45 0.45 0.75 0.75 0.45 0.45 0.75 0.75
Propylene glycol 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5
Ethanol 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25
Methylparaben 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01
Propylparaben 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005
Glutaraldehyde 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05
Purified water (q.s) 20 20 20 20 20 20 20 20
Final formulation code: (Table 3)
Ingredients
(% w/w)
F1 F2 F3 F4 F5 F6 F7 F8
Aceclofenac (mg) 100 100 100 100 100 100 100 100
CMC 0.5 0.5 0.5 0.5 - - - -
Carbopol 934 - - - - 0.25 0.25 0.25 0.25
Light liquid paraffin 2.5 3.75 2.5 3.75 2.5 3.75 2.5 3.75
Tween 20 0.3 0.3 0.5 0.5 0.3 0.3 0.5 0.5
Span 20 0.45 0.45 0.75 0.75 0.45 0.45 0.75 0.75
Propylene glycol 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5
Ethanol 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25
3. Mahesh B et al / Journal of Pharmacreations Vol-3(1) 2016 [90-98]
92
Methylparaben 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01
Propylparaben 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005
glutaraldehyde 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05
Purified water (q.s) 50 50 50 50 50 50 50 50
Formulation of Aceclofenac emulgel
Gel preparation
The composition of Aceclofenac emulgel 10%
w/w was shown in the formulation code table. The
carbopol gel was prepared by dispersing 1.25g of
carbopol 934 in purified water with constant stirring
at a moderate speed and soaked overnight. The gel
was obtained by neutralizing the dispersion with tri
ethanol amine and pH is adjusted to 6.5 and purified
water was added to adjust the weight to 50g.In case
of Carboxy Methyl cellulose gel was prepared by
dispersing CMC in hot purified water (800
C) and the
dispersion was cooled, then weight was adjusted to
50g with purified water.
Emulsion preparation
The oil phase of emulsion was prepared by dissolving
span 20 in light liquid paraffin and heated upto 700
-
800
C.Aqueous phase was prepared by dissolving
tween 20 and drug in 5ml ethanol and heated upto
700
-800
C. Methylparaben, propylparaben were mixed
in propylene glycol and glutaraldehyde and this
added this mixture was dissolved in aqueous
phase.Then oil phase was mixed slowly with aqueous
phase and final volume is made with purified water.
Emulgel preparation
The obtained emulsion was mixed with the gel and
weight was adjusted to 50g with water and subjected
to homogenization for 45 minutes to get aceclofenac
emulgel 10% w/w.
RESULTS
Identification of authenticity of Aceclofenac
pure drug
Physical appearance
Physical appearance of the drug was examined by
organoleptic properties and results were obtained as
follows:
Color: White or almost white
Odor: Odorless
Taste: Bitter
State: crystalline powder
FTIR spectroscopy
Figure: 1 FTIR of Aceclofenac pure drug
4. Mahesh B et al / Journal of Pharmacreations Vol-3(1) 2016 [90-98]
93
Figure: 2 FTIR of Aceclofenac emulgel optimized formulation
Evaluation parameters
Physical appearance: (Table4 )
Formulation code Color Homogeneity Consistency Phase separation
F1 Creamy white Homogenous Smooth Not occured
F2 Creamy white Homogenous Smooth Not occurred
F3 Creamy white Homogenous Smooth Not occured
F4 Creamy white Homogenous Smooth Not occured
F5 Creamy white Homogenous Smooth Not occured
F6 Creamy white Homogenous Smooth Not occured
F7 Creamy white Homogenous Smooth Not occured
F8 Creamy white Homogenous Smooth Not occured
pH determination: (Table5 )
Sl.no Formulation code pH
1 F1 6.4±0.43
2 F2 6.1±0.63
3 F3 6.7±0.25
4 F4 6.0±0.72
5 F5 6.3±0.11
6 F6 6.4±0.24
7 F7 6.7±0.88
8 F8 6.5±0.02
6. Mahesh B et al / Journal of Pharmacreations Vol-3(1) 2016 [90-98]
95
9 60.02 52.32 42.62 49.83 68.56 40.65 77.54 45.65
10 68.32 59.82 49.53 52.64 76.85 46.74 83.45 52.25
11 74.60 63.25 53.62 57.45 80.25 50.15 85.05 55.48
12 76.77 69.53 60.31 62.06 84.32 58.09 89.97 63.42
Figure: 3In vitro drug permeation graph
7 Drug release kinetics
Fig no: 4 Zero order graph of optimized formulation
0
10
20
30
40
50
60
70
80
90
100
0 2 4 6 8 10 12 14
cumulative%drugrelease
time in hrs
F1
F2
F3
F4
F5
F6
F7
F8
y = 7.7575x + 4.6718
R² = 0.9804
0
20
40
60
80
100
120
0 2 4 6 8 10 12 14
%
C
D
R
TIME IN HRS
ZERO ORDER
7. Mahesh B et al / Journal of Pharmacreations Vol-3(1) 2016 [90-98]
96
Fig no:5 First order graph of optimized formulation
Fig no:6 Higuchi graph of optimized formulation
y = -0.1423x + 2.2729
R² = 0.6742
0
0.5
1
1.5
2
2.5
0 2 4 6 8 10 12 14
LOG%DRUGREMAINED
TIME
FIRST ORDER
y = 29.573x - 15.32
R² = 0.9545
-40
-20
0
20
40
60
80
100
0 1 2 3 4
%
C
D
R
SQUARE ROOT OF TIME
HIGUCHI PLOT
8. Mahesh B et al / Journal of Pharmacreations Vol-3(1) 2016 [90-98]
97
Fig no:7 Peppas graph of optimized formulation
Table no:10 Release kinetics of optimized formulation
ZERO FIRST HIGUCHI PEPPAS
% CDR Vs T Log % Remain Vs T %CDR Vs √T Log C Vs Log T
Slope 7.757527473 -0.14226443 29.57298225 1.343816675
Intercept 4.671758242 2.272945911 -15.3200226 0.639814304
Correlation 0.990176129 -0.82109574 0.976979985 0.908588553
R 2 0.980448767 0.674198227 0.95448989 0.825533159
Scanning electron microscope study
Figure: Best Emulgel formulation (F7)
Figure: 8SEM of Best formulation (F7)
y = 1.3438x + 0.6398
R² = 0.8255
0
0.5
1
1.5
2
2.5
0 0.2 0.4 0.6 0.8 1 1.2
L
O
G
%
C
D
R
LOG TIME
PEPPAS
9. Mahesh B et al / Journal of Pharmacreations Vol-3(1) 2016 [90-98]
98
DISCUSSION
The optimized formulation F7 showed a shear
thinning with thixotropic property with better
spreadability, viscosity and in-vitro permeability
compared to other formulations. In the study it was
observed that the concentrations of tween20 spann 20
and light liquid paraffin has shown effect on
viscosity, spreadability and in-vitro drug
permeability. Increased amount of liquid paraffin
showed suppress activity of tween 20 and span
20.The formulation F7 showed the 89.97% drug
release.
CONCLUSION
Aceclofenac is categorized as anti-arthritic drug
and is successfully used in the treatment of arthritis.
Aceclofenac is a lipophylic anti-arthritic drug with
biological half life 4hrs with oral bioavailability 60-
70%. Aceclofenac causes severe gastrointestinal
related toxicities associated with oral administration.
So to overcome this problem it was alternatively
developed for topical route of administration.
Aceclofenac maximum wavelength is determined by
UV-Visible spectrophotometer using 6.8 pH
phosphate buffer and was detected to be 275 nm.
Aceclofenac emulgel was formulated using light
liquid paraffin as oil phase and emulsifying agents
tween 20 and span 20 for emulsion and incorporated
into gel using CMC and carbopol 934 polymers in
different ratios. The optimized formulation F7
showed a shear thinning with thixotropic property
with better spreadability, viscosity and in-vitro
permeability compared to other formulations. In the
study it was observed that the concentrations of
tween20 spann 20 and light liquid paraffin has shown
effect on viscosity, spreadability and in-vitro drug
permeability. Increased amount of liquid paraffin
showed suppress activity of tween 20 and span 20.
The surface morphology of the optimized
formulation was observed by Scanning Electron
Microscopic study. Thus Aceclofenac emulgel which
could increase the drug permeability across the skin
and fast release of the drug could be successfully
achieved.
REFERENCES
[1]. Arunachalam A et al. Transdermal drug delivery systems: A review. Current Pharma Res 2010; 1(1): 70-81.
[2]. Meera C Singh, Ajinkya S Naik, Sawant SD. Transdermal drug delivery system with major emphasis on
transdermal patches. J Pharm Res 2010; 3(10): 2537-2543.
[3]. Robinson JR, Lee VH. Controlled drug delivery fundamentals and applications. 2nd ed. New Delhi: CBS
Publishers & Distributors; 2005; vol 29: 523-552.
[4]. KottaKranthi Kumar, Sasikanth K, Sabareesh M, Dorababu N. Formulation and evaluation of diacerein
cream. Asian J Pharm Clinical Res 2011; 4(2): 93-98.
[5]. Jonathan Hadgraft, Richard H Guy. Transdermal drug delivery: development issues and research initiative.
Marcel Dekker. Vol 35: 1-16.
[6]. Divyesh Patel, Nirav Patel, MeghalParmar, NavpreetKaur. Transdermal drug delivery system: review. Int J
BiopharmToxicol Res 2011; 1(1): 61-80.
[7]. VirendraYadav. Transdermal drug delivery system: review. Int J Pharm Sci Res2012; 3(2): 376-382.
[8]. Ankush I Shembale, Dipak K Borole, Rajesh T Lohiya. Useful permeation enhancers for transdermal drug
delivery: A review. Int J Pharma Res Dev 2010; 2(5):1-6.
[9]. Loyd VA, Nicholas GP, Ansel HC. Pharmaceutical dosage forms and drug delivery systems. 8th ed.
Philadelphia: Lippincott Williams and Wilkins 2005; 298-315.
[10]. Bhavsar JD, Brahambhatt VG, Patel MR, Patel KR, Patel NM. Review article: Novel approaches in
semisolids. Int J Pharm World Res 2011; 2(1): 1-22.
[11]. Patil Bharat, MandoreParesh, Sharma RK, Tekade BW, Thakre VM, Patil VR. A review: novel advances in
semisolid dosage form and patented technology in semisolid dosage forms. Int J Pharm Tech Res 2011; 3(1):
420-430
[12]. RachitKhullar, Saini S, Seth N, Rana AC. Emulgels: a surrogate approach for topically used hydrophobic
drugs. Int J Pharm Bio Sci 2011; 1(3): 117-128.
[13]. Joshi Baibhav, Singh Gurupreet, Rana AC, SainiSeema, Single Vikas. Emulgel: a comprehensive review on
the recent advances in topical drug delivery. Int Res J Pharm 2011; 2(11): 66-70.