The document discusses the isolation and purification of flavonoids, which are natural polyphenolic compounds found in various plant sources. It outlines the structure of flavonoids, their roles in plant development and defense, and the methods for extracting them using different solvents. The extraction involves boiling plant material and purification through fractional crystallization.

1. Topic
Introduction, Isolation and Purification of Flavonoids
Presented by
Swapnil G. Ugle
M. Pharmacy 1st Semister
Department of Pharmaceutical Sciences, Rashtrasant Tukdoji Maharaj
Nagpur University, Nagpur

2. INDEX
 Introduction
 Structure of flavonoid
 Isolation and purification of flavonoid

3. Introduction
 Flavonoids are group of natural substances with variable
phenolic structures.
 Are found in fruits, vegetables, grains, bark, roots, stems,
flowers, tea and wine.
 These are secondary metabolites of plants.
 Polyphenolic 15 carbon containing compounds.
 Flavonoids regulate plant development, pigmentation,
and UV protection.
 And roles in defence and signalling between plants and
microorganisms.

4. Structure of Flavonoids
Flavonoid is 15 carbon
compound.
C6C3C6
Two rings are aromatic and
middle ring contain oxygen.

5. Basically flavonoids are
polyphenolic compounds.

7. Isolation and purification of flavonoids
 Powdered plant material is extracted with various solvents.
 Various solvents are used according to types of flavonoides like
CHCl3 , Diethyl ether, ethyl acetate etc.
 All solvent used for extraction are semipolar or nonpolar.
 More polar flavonoids are extracted with Alcohol or aqueous
alcohol.
 Fractional crystallization is employed for purification of extract.

8. Genaral extraction
method: Powdered plant material
Extraction with boiling water
Aqueous extract
Trated with lead acetate
Precipitation with tannin Supernatant liquid containing
flavonoids
Dilute with water + HCl
+ boil for 1 hour
Precipitate of sugar free
flavonoid
Ppt extract with alcohol + purified
with fractional crystallization
Flavonoid

10. References:
https//www.slideshare.net/roshniannbaby/flavanoids
https://www.google.com/search?client=ms-android-
xiaomi&hl=en&sxsrf=AJOqlzXtRGrpkcJt8_hFWvNdhuRMUheozw:16
76813709827&q=flavonoids+slideshare&tbm=isch&sa=X&ved=2ah
UKEwjx-
5zs2aH9AhWI8zgGHQEKBvQQ0pQJegQICBAB&biw=360&bih=559&
dpr=2#

11. THANK YOU