This document provides information about eye donation and pledging eyes after death. It outlines the process of registering with an eye bank and the criteria for who can and cannot donate eyes. The key steps in removing eyes from a donor are described, including transporting the corneas to the eye bank within 6 hours of death. Methods of short-term storage like moist chamber and McCarey Kaufman medium allow preservation for up to 4 days, while intermediate and long-term storage options can maintain corneas for weeks to years by reducing hydration or cryopreservation. Donated corneas can be used for keratoplasty procedures to restore vision, remove infections, or maintain the integrity of the eyeball.

1. EYE DONATION

2. INCIDENCE OF BLINDNESS

3. EYE PLEDGING
 Eyes should not be removed from a living person
 Eyes should not be purchased or sold
 Individuals can register in the nearest eye bank for
donating their eyes after death.
 Eyes are collected after getting death information
and consent from nearest relatives.

4. PLEDGE FORM
 Name :
 Gender :
 Date of Birth : Submit (mm/dd/yyyy)
 e-mail id :
 Address :
 Country :
 State :
 City :
 Pincode :
 Phone No (Residence) :
 Mobile :
The information furnished above is correct to my knowledge
I PLEDGE

5. WHO CAN DONATE
 Any age
 Any sex
 Any religion
 Even if not pledged while alive
 Spectacle wearers
 Undergone cataract or other eye surgeries
 Diabetics & hypertensives

6. WHO CANNOT
 HIV +
 HEPATITIS B , C
 Rabies
 Syphilis
 Creutzfeldt Jacob Disease
 Alzeimer’s Disease
 Septicemia
 Leukemia
 Death From Unknown Cause

7.  Corneal diseases
 Intraocular tumours eg. Retinoblastoma,melanoma
 Iridocyclitis
 Endophthalmitis

8. DONOR REMOVAL

9. TIME OF REMOVAL
:
 Eyes must be collected within 6 hours of death.

10. BEFORE REMOVAL
 dgg

11. INFORMATION NOTED
 AGE OF DONOR
 TIME OF DEATH
 CAUSE OF DEATH

12. Methods of REMOVAL
 ENUCLEATION

13.  CORNEO-SCLERAL BUTTON EXCISION

14.  5 ml blood withdrawn from donor to screen for
infectious diseases like HIV, Hepatitis

15. TRANSPORT TO EYE BANK
MOIST CHAMBER METHOD
 Glass bottles with metal stand

16. EVALUATION OF DONOR CORNEA
 SLIT LAMP BIOMICROSCOPY
 SPECULAR MICROSCOPY- endothelial cell count

17. GRADING OF CORNEA

18. STORAGE
 SHORT TERM (2-4 days)
 INTERMEDIATE TERM ( 2 weeks)
 LONG-TERM (months-years)

19. SHORT TERM
 MOIST – CHAMBER METHOD (2 Days)
 McCAREY KAUFMAN MEDIUM (4days)

20. MOIST – CHAMBER METHOD
 The whole donor eye is kept in a sterile jar filled with
saturated moist atmosphere at 4°C
 Upto 2 days
 Advantage – simplest, cheap, esp in developing
countries.
 Disadvantage – intact eye, hence endothelium
exposed to aqueous changes.

21. McCAREY KAUFMAN MEDIUM
 Described by McCarey and Kaufman
Contents
 Tissue culture TC-199
 5% dextran-40
 HEPES buffer
 Gentamycin
 Pink in colour
 Upto 4 days.
 Only Corneoscleral button

22. INTERMEDIATE TERM
 Corneal storage media containing chondroitin
sulfate
 Enhanced corneal dehydration.
 OPTISOL
 K-SOL
 DEXSOL
 Upto 2 weeks.

23. LONG TERM
 ORGAN – CULTURE METHOD
Corneas are incubated in tissue culture medium
supplemented with fetal calf serum, antibiotics, and
antimycotics at 30–37°C.
 Upto 30 days
 CRYOPRESERVATION:
Freezing the donor cornea stored in liquid nitrogen.
Upto 1 year

24. UTILIZATION OF DONOR CORNEA
 KERATOPLASTY
Penetrating – full thickness graft
Lamellar – partial thickness graft
 Optical – to restore vision
 Therapeutic – remove infected tissues in non-healing
ulcers
 Tectonic – restore integrity of eyeball
 Cosmetic