Percent Composition in
Plants
Basilio, Chavez, Escudero, Evidente, Lirio
Introduction
Plants are composed of water, organic and
inorganic matter.
   Fresh plant matter is about 80-95% water.

Water is the principal constituent of plants and an
essential component for the maintenance of life,
growth and development.
Introduction
Organic matter
   Hydrogen, Oxygen, Carbon
   Drawn from the soil and the air, but generally
   through the absorption done by the roots.

Inorganic Matter
   Phosphorus, Sulfur, Potassium, Calcium,
   Magnesium, Silicon and other elements.
   From the soil
Introduction
Determine the percent composition of water,
organic and inorganic constituents of plants by
the determination of fresh, dry and ash weight.
Methodology
two replicates, one species (flowering plant)

wash &blot

get fresh weight (record)

wrap in paper (label)

dry in oven (not >80ºC)

get dry weight (record)
Methodology
cut dried specimen

put in pre-weighed crucible

place in muffle furnace (550ºF)

cool & weigh ash content
Results
Table 1: Percent Composition of Water, Organic and Inorganic
Constituents in Vernonia sp.
Results
Table 2: Percent Composition of Water, Organic and
Inorganic Constituents in Catharanthus roseus.
Results
Table 3: Percent Composition of Water, Organic and
Inorganic Constituents in Catharanthus roseus.
Results
Table 4: Percent Composition of Water, Organic and
Inorganic Constituents in Tridax sp.
Discussion
Chemicals and substances that function to maintain
  an internal balance in the body compose the
  cells and tissues of living organisms.

            water,
            organic matter, and
            inorganic matter
Water
Depending on the type of plant, the
water content in most fruits and
vegetables accounts for approximately
70 to 95 percent of their weight.
Parameter            Replicate 1   Replicate 2   Average
Fresh weight (FW), gms         3.7           2.0        2.85

 Dry weight (DW), gms          1             0.6         0.8

 Ash weight (AW), gms          0.1           0.1         0.1
  Percent water {(FW-
                            72.97%          70%        71.49%
     DW)/FW} X100
   Percent dry matter
                            27.03%          30%        28.52%
    {(DW/FW)X100}
Percent inorganic matter
                            2.703%          5%         3.85%
    (AW/FW)X100
Percent organic matter
                            24.33%          25%        24.67%
     %DW-%AW
Water is the major
component of living
cells and
constitutes more
than 90% of
protoplasm by
volume and weight
•medium for the
conduction and
distribution of nutrients
and other ions

• maintaining turgor
pressure
Vernonia sp.

A herbaceous mesophyte
                          Source:
                          http://upload.wikimedia.org/wikipedia/c
                          ommons/3/3d/Vernonia_cinerea.jpg
Organic
matter
Carbon-containing
compounds
    •carbohydrates,

    • lipids,

    • nucleic acids, and

    •proteins
Parameter            Replicate 1   Replicate 2   Average
Fresh weight (FW), gms         3.7           2.0        2.85

 Dry weight (DW), gms          1             0.6         0.8

 Ash weight (AW), gms          0.1           0.1         0.1
  Percent water {(FW-
                            72.97%          70%        71.49%
     DW)/FW} X100
   Percent dry matter
                            27.03%          30%        28.52%
    {(DW/FW)X100}
Percent inorganic matter
                            2.703%          5%         3.85%
    (AW/FW)X100
Percent organic matter
                            24.33%          25%        24.67%
     %DW-%AW
Pathway of lignin biosynthesis in angiosperm trees.
Source: http://www.nature.com/nbt/journal/v20/n6/fig_tab/nbt0602-557_F1.html
Source: http://mpnforum.files.wordpress.com/2012/01/genome-project-cartoon.png
Inorganic matter
 Macronutrients

 Micronutrients
Parameter            Replicate 1   Replicate 2   Average
Fresh weight (FW), gms         3.7           2.0        2.85

 Dry weight (DW), gms          1             0.6         0.8

 Ash weight (AW), gms          0.1           0.1         0.1
  Percent water {(FW-
                            72.97%          70%        71.49%
     DW)/FW} X100
   Percent dry matter
                            27.03%          30%        28.52%
    {(DW/FW)X100}
Percent inorganic matter
                            2.703%          5%         3.85%
    (AW/FW)X100
Percent organic matter
                            24.33%          25%        24.67%
     %DW-%AW
Conclusion
Why study the percent composition of plants
   understand physiology and morphology
   environmental conditions
   health condition
Conclusion
Generally, plants are composed of:
   Water
   organic matter
   inorganic matter
Conclusion

Vernonia sp. (Asteraceae)
   water (71.49%) > organic matter (24.67%) >
   inorganic matter (3.85%)
Experiment 3 percent composition in plants

Experiment 3 percent composition in plants

  • 1.
    Percent Composition in Plants Basilio,Chavez, Escudero, Evidente, Lirio
  • 2.
    Introduction Plants are composedof water, organic and inorganic matter. Fresh plant matter is about 80-95% water. Water is the principal constituent of plants and an essential component for the maintenance of life, growth and development.
  • 3.
    Introduction Organic matter Hydrogen, Oxygen, Carbon Drawn from the soil and the air, but generally through the absorption done by the roots. Inorganic Matter Phosphorus, Sulfur, Potassium, Calcium, Magnesium, Silicon and other elements. From the soil
  • 4.
    Introduction Determine the percentcomposition of water, organic and inorganic constituents of plants by the determination of fresh, dry and ash weight.
  • 5.
    Methodology two replicates, onespecies (flowering plant) wash &blot get fresh weight (record) wrap in paper (label) dry in oven (not >80ºC) get dry weight (record)
  • 6.
    Methodology cut dried specimen putin pre-weighed crucible place in muffle furnace (550ºF) cool & weigh ash content
  • 7.
    Results Table 1: PercentComposition of Water, Organic and Inorganic Constituents in Vernonia sp.
  • 8.
    Results Table 2: PercentComposition of Water, Organic and Inorganic Constituents in Catharanthus roseus.
  • 9.
    Results Table 3: PercentComposition of Water, Organic and Inorganic Constituents in Catharanthus roseus.
  • 10.
    Results Table 4: PercentComposition of Water, Organic and Inorganic Constituents in Tridax sp.
  • 11.
    Discussion Chemicals and substancesthat function to maintain an internal balance in the body compose the cells and tissues of living organisms.  water,  organic matter, and  inorganic matter
  • 12.
    Water Depending on thetype of plant, the water content in most fruits and vegetables accounts for approximately 70 to 95 percent of their weight.
  • 13.
    Parameter Replicate 1 Replicate 2 Average Fresh weight (FW), gms 3.7 2.0 2.85 Dry weight (DW), gms 1 0.6 0.8 Ash weight (AW), gms 0.1 0.1 0.1 Percent water {(FW- 72.97% 70% 71.49% DW)/FW} X100 Percent dry matter 27.03% 30% 28.52% {(DW/FW)X100} Percent inorganic matter 2.703% 5% 3.85% (AW/FW)X100 Percent organic matter 24.33% 25% 24.67% %DW-%AW
  • 14.
    Water is themajor component of living cells and constitutes more than 90% of protoplasm by volume and weight •medium for the conduction and distribution of nutrients and other ions • maintaining turgor pressure
  • 15.
    Vernonia sp. A herbaceousmesophyte Source: http://upload.wikimedia.org/wikipedia/c ommons/3/3d/Vernonia_cinerea.jpg
  • 16.
    Organic matter Carbon-containing compounds •carbohydrates, • lipids, • nucleic acids, and •proteins
  • 17.
    Parameter Replicate 1 Replicate 2 Average Fresh weight (FW), gms 3.7 2.0 2.85 Dry weight (DW), gms 1 0.6 0.8 Ash weight (AW), gms 0.1 0.1 0.1 Percent water {(FW- 72.97% 70% 71.49% DW)/FW} X100 Percent dry matter 27.03% 30% 28.52% {(DW/FW)X100} Percent inorganic matter 2.703% 5% 3.85% (AW/FW)X100 Percent organic matter 24.33% 25% 24.67% %DW-%AW
  • 18.
    Pathway of ligninbiosynthesis in angiosperm trees. Source: http://www.nature.com/nbt/journal/v20/n6/fig_tab/nbt0602-557_F1.html
  • 20.
  • 21.
  • 22.
    Parameter Replicate 1 Replicate 2 Average Fresh weight (FW), gms 3.7 2.0 2.85 Dry weight (DW), gms 1 0.6 0.8 Ash weight (AW), gms 0.1 0.1 0.1 Percent water {(FW- 72.97% 70% 71.49% DW)/FW} X100 Percent dry matter 27.03% 30% 28.52% {(DW/FW)X100} Percent inorganic matter 2.703% 5% 3.85% (AW/FW)X100 Percent organic matter 24.33% 25% 24.67% %DW-%AW
  • 24.
    Conclusion Why study thepercent composition of plants understand physiology and morphology environmental conditions health condition
  • 25.
    Conclusion Generally, plants arecomposed of: Water organic matter inorganic matter
  • 26.
    Conclusion Vernonia sp. (Asteraceae) water (71.49%) > organic matter (24.67%) > inorganic matter (3.85%)

Editor's Notes

  • #13 Singkamas, Pachyrhizuserosus86-90% waterWatermelon, ‘melon’, apples, apricot, celery carrots, berries
  • #15 Turgor pressure = keeps plants to remain upright and in shape
  • #16 Mesophytic plants havewater rather than organic matter in high amounts.
  • #17 Cellulose, the basic structural component of plant cell walls, is a polysaccharide that composes roughly 33% of all vegetable matter.
  • #19 carbohydrates that function as structural materials for the plant also include hemicelluloses, pectin, and lignin
  • #20 Lipids and proteins compose the cell’s plasma membranePreteins also serve tofacilitate basic physiological functions of the plantchannels in regulating water and ions that enter and leave the cellWhile lipids are present as cutin and suberin
  • #21 Nucleic acids are present as DNA and RNA 
  • #22 Macro – present in high quantities; Micro – present in low amounts
  • #24 Seventy percent of total ash content is a mixture of calcium and phosphorus and 1% is attributed to wet body mass (Hallare, n.d.). - Mainly function as cofactors in certain biochemical reactions