Exosomes Biogenesis&Therapeutics
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Exosomes have specialized functions and play a key role in different physiological processes and pathological conditions. Consequently, exosomes have attracted increasing attention in their clinical applications for prognosis, https://www.creative-biolabs.com/exosome/applications.htm
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Exosomes - Diagnostics and Therapeutics
This document discusses exosomes, which are extracellular vesicles that originate inside cells and are released outside. It provides background on the discovery of exosomes and their structure. Exosomes are 40-150nm in size and have a phospholipid bilayer. They are secreted by various immune cells, epithelial cells, and are present in many body fluids. The document outlines their biogenesis pathway and composition. It discusses the many potential roles and applications of exosomes in diagnostics and therapeutics for diseases like cancer, neurological disorders, and infectious diseases. Exosomes show promise as novel biomarkers for diagnosis and as vehicles for drug delivery.
Exosome
1. Extracellular vesicles (EVs) such as exosomes and microvesicles are naturally released from cells and have specialized functions including intercellular communication.
2. EVs contain proteins, mRNAs, miRNAs and other molecules. They have potential applications as drug delivery vehicles due to their ability to target specific cells and tissues with low toxicity.
3. The document describes different types of EVs, their composition, biogenesis, isolation methods, and potential use for cancer therapy through targeted drug delivery via surface proteins on EVs.
Exosome isolation and characterization
This document discusses exosome isolation and characterization. It begins with an introduction to exosomes and their biogenesis. It then discusses two challenges in the field: simplifying isolation procedures and distinguishing exosomes from other extracellular vesicles. Six common exosome isolation strategies are described: ultracentrifugation, ultrafiltration, immunoaffinity capture, polymer precipitation, size-exclusion chromatography, and microfluidic techniques. The document concludes by outlining several characterization methods, including electron microscopy, dynamic light scattering, protein quantification assays, and assessing the presence of exosomal markers through western blotting.
Exosome applications
Exosomes have specialized functions and play key roles in physiological and pathological processes. As a result, exosomes show potential for clinical applications in prognosis, diagnosis, drug delivery, and vaccine development. Exosomes can serve as diagnostic biomarkers found in bodily fluids and allow for minimally invasive approaches. They also show promise as drug delivery vehicles due to their biocompatibility, stability, and low toxicity. Additionally, exosomes may function as therapeutic cell-free vaccines by stimulating immune responses against tumors or infections. Stem cell derived exosomes also demonstrate potential in regenerative medicine applications.
Exosome and cancer
Exosomes are smallest extracellular vesicles of size 30 to 100 nm originated from late endosomes. These are released by broad array of cells including B‐ cells, cells, dendritic cells (DCs), T‐cells, epithelial cells,
platelets and many more.
Exosomes.final.pptx
Exosomes are membrane-bound extracellular vesicles that are 30-150 nm in diameter and produced by cells. They contain proteins, lipids, and nucleic acids from their cell of origin. The small size of exosomes makes them challenging to isolate and analyze. Common methods for isolating exosomes include differential ultracentrifugation, ultrafiltration, polyethylene glycol precipitation, immunoaffinity capture, microfluidics, and size-exclusion chromatography. Size-exclusion chromatography is considered an ideal method as it produces relatively pure exosome isolates without excessive sample volumes. Exosomal RNA and membranes can be labeled with fluorescent dyes like green fluorescent cell stain and ceramide for visualization and tracking.
Exosomes lecture
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EXTRACELLULAR VESICLES IN CANCER
This document summarizes a case study on using exosomes for cancer therapy. Key points include:
1) Exosomes were loaded with the anticancer drug paclitaxel (PTX) using incubation, electroporation, or sonication methods. Sonication provided the greatest drug loading capacity and stability.
2) Loaded exosomes (exoPTX) accumulated more in drug-resistant cancer cells than free drug. ExoPTX also overcame P-glycoprotein mediated drug resistance.
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Exosomes - Diagnostics and Therapeutics
This document discusses exosomes, which are extracellular vesicles that originate inside cells and are released outside. It provides background on the discovery of exosomes and their structure. Exosomes are 40-150nm in size and have a phospholipid bilayer. They are secreted by various immune cells, epithelial cells, and are present in many body fluids. The document outlines their biogenesis pathway and composition. It discusses the many potential roles and applications of exosomes in diagnostics and therapeutics for diseases like cancer, neurological disorders, and infectious diseases. Exosomes show promise as novel biomarkers for diagnosis and as vehicles for drug delivery.
Exosome
1. Extracellular vesicles (EVs) such as exosomes and microvesicles are naturally released from cells and have specialized functions including intercellular communication.
2. EVs contain proteins, mRNAs, miRNAs and other molecules. They have potential applications as drug delivery vehicles due to their ability to target specific cells and tissues with low toxicity.
3. The document describes different types of EVs, their composition, biogenesis, isolation methods, and potential use for cancer therapy through targeted drug delivery via surface proteins on EVs.
Exosome isolation and characterization
This document discusses exosome isolation and characterization. It begins with an introduction to exosomes and their biogenesis. It then discusses two challenges in the field: simplifying isolation procedures and distinguishing exosomes from other extracellular vesicles. Six common exosome isolation strategies are described: ultracentrifugation, ultrafiltration, immunoaffinity capture, polymer precipitation, size-exclusion chromatography, and microfluidic techniques. The document concludes by outlining several characterization methods, including electron microscopy, dynamic light scattering, protein quantification assays, and assessing the presence of exosomal markers through western blotting.
Exosome applications
Exosomes have specialized functions and play key roles in physiological and pathological processes. As a result, exosomes show potential for clinical applications in prognosis, diagnosis, drug delivery, and vaccine development. Exosomes can serve as diagnostic biomarkers found in bodily fluids and allow for minimally invasive approaches. They also show promise as drug delivery vehicles due to their biocompatibility, stability, and low toxicity. Additionally, exosomes may function as therapeutic cell-free vaccines by stimulating immune responses against tumors or infections. Stem cell derived exosomes also demonstrate potential in regenerative medicine applications.
Exosome and cancer
Exosomes are smallest extracellular vesicles of size 30 to 100 nm originated from late endosomes. These are released by broad array of cells including B‐ cells, cells, dendritic cells (DCs), T‐cells, epithelial cells,
platelets and many more.
Exosomes.final.pptx
Exosomes are membrane-bound extracellular vesicles that are 30-150 nm in diameter and produced by cells. They contain proteins, lipids, and nucleic acids from their cell of origin. The small size of exosomes makes them challenging to isolate and analyze. Common methods for isolating exosomes include differential ultracentrifugation, ultrafiltration, polyethylene glycol precipitation, immunoaffinity capture, microfluidics, and size-exclusion chromatography. Size-exclusion chromatography is considered an ideal method as it produces relatively pure exosome isolates without excessive sample volumes. Exosomal RNA and membranes can be labeled with fluorescent dyes like green fluorescent cell stain and ceramide for visualization and tracking.
Exosomes lecture
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EXTRACELLULAR VESICLES IN CANCER
This document summarizes a case study on using exosomes for cancer therapy. Key points include:
1) Exosomes were loaded with the anticancer drug paclitaxel (PTX) using incubation, electroporation, or sonication methods. Sonication provided the greatest drug loading capacity and stability.
2) Loaded exosomes (exoPTX) accumulated more in drug-resistant cancer cells than free drug. ExoPTX also overcame P-glycoprotein mediated drug resistance.
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The document provides information about exosomes including their history, structure, biogenesis, types, isolation techniques, applications, and conclusions. Exosomes are extracellular vesicles released by cells that carry proteins, mRNAs, and other biomolecules. They were first discovered in 1983 and are 30-100nm in diameter. Exosomes have potential applications as biomarkers for disease detection and therapeutic delivery vehicles due to their ability to cross membranes and stimulate tissue regeneration. Further research is still needed to better understand exosome biology and utilize their full potential.
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tumor immunology.pptx
- Cancer remains a leading cause of death globally, with an estimated 12.7 million cases expected to increase to 21 million by 2030.
- Tumors can be detected through abnormal cell proliferation caused by genetic mutations or loss of growth control.
- The immune system responds to tumor antigens expressed by cancer cells and tumor-associated antigens shared between tumors induced by the same virus.
- However, tumors have developed multiple mechanisms to evade immune surveillance such as low immunogenicity, antigen modulation, immune suppression, and inducing lymphocyte apoptosis.
Tumor Dormancy
H. Kim Lyerly, M.D., FACS, discusses research in tumor dormancy in breast cancer, including the role of disseminated tumor cells, bone marrow, and the potential for immune responses to control or prevent recurrences. Dr. Lyerly is Director of the Center of Applied Therapeutics at Duke University.
Tumor Dormancy
This document discusses tumor dormancy and mechanisms by which cancer cells can evade immune detection and elimination. It describes how some cancer cells enter a dormant state after disseminating from primary tumors and residing long-term in bone marrow niches without proliferating. These dormant disseminated tumor cells can later reactivate and form metastases. The document also outlines a mouse model using E0771 breast cancer cells expressing a model antigen (ovalbumin) to study interactions between dormant tumor cells in bone marrow and antigen-specific T cells, with the goal of developing non-toxic therapies to eliminate dormant tumor cells or prevent their outgrowth into metastases.
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ROLE OF IMMUNE CELLS IN CANCER AND TARGETING IMMUNE CELLS FOR CANCER THERAPY
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Genome Editing Introduction
Genome editing is a method of making specific changes to the DNA of a cell or organism. An enzyme scissors the DNA at a specific sequence, and when this is repaired by the cell, a change or ‘edit’ is made to the sequence.
https://www.creative-biolabs.com/gene-therapy/approaches-to-genome-editing.htm
Oncolytic Virus and Immunotherapy
Oncolytic viruses (OVs) provide a diverse platform for immunotherapy; they act as in situ vaccines, and can be armed with immuno-modulatory transgenes or combined with other immunotherapie. Taking advantage of the OncoVirapy™ platform, Creative Biolabs provides high-quality oncolytic virus therapy development services for clients globally.
https://www.creative-biolabs.com/oncolytic-virus/oncolytic-virotherapy-development-for-combination-therapy-with-cancer-immunotherapy.htm
Introduction of SARS-CoV-2
SARS-CoV-2 was first identified in Wuhan, China in late 2019 and has since spread to over 200 countries. It belongs to the coronavirus family and causes the disease COVID-19. The virus enters cells through the ACE2 receptor and replicates its RNA and proteins before being released to infect other cells. Potential treatment strategies include using existing antiviral drugs, developing specific drugs that target the virus genome or proteins, and enhancing immune responses. Ongoing research focuses on antibody development, diagnostic assays, vaccines, and other medical countermeasures to treat and prevent the spread of SARS-CoV-2.
SARS-CoV-2 Introduction_Creative Biolabs
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Equipped with cutting-edge techniques and vast experience, Creative Biolabs is keeping up with current research hotspots and integrating all resources to guarantee high-quality services (including but not limiting to): Antiviral Drug Discovery, Vaccine Discovery, Evaluation Platforms, In Vitro Diagnostic (IVD) Immunoassays.
https://sars-cov-2.creative-biolabs.com/novel-coronavirus-sars-cov2.htm
SARS-CoV-2 Introduction_Creative Biolabs
SARS-CoV-2 was first identified in Wuhan, China in late 2019 and has since spread to over 200 countries. It belongs to the coronavirus family and causes the disease COVID-19. The virus enters cells through the ACE2 receptor and replicates its RNA and proteins before being released to infect other cells. Potential treatment strategies include using existing antiviral drugs, developing specific drugs that target the virus genome or proteins, and enhancing immune responses. Ongoing research focuses on antibody development, diagnostic assays, vaccines, and other medical countermeasures to treat and prevent the spread of SARS-CoV-2.
Complement Therapeutics_Creative Biolabs
Creative Biolabs is a leading service provider in the complement therapeutics field in the US. Our one-stop drug discovery platforms and experienced technicians are dedicated in helping you develop therapeutic candidates, diagnostic reagents.
https://www.creative-biolabs.com/complement-therapeutics/
Anti dengue-virus-antibody-products-and-services
Dengue virus (DENV) can cause dengue fever. It is a mosquito-borne, single positive-stranded RNA virus of Flaviviridae. In recent decades, the outbreak of dengue has grown dramatically in the world. Nowadays, dengue is endemic in more than 100 countries.
https://viroantibody.creative-biolabs.com/category-dengue-virus-371.htm
The development of oncolytic viruses—Creative Biolabs
With a better understanding of cancer biology and virology, oncolytic virus therapy is becoming a promising treatment modality for tumor targeting and offers unique opportunities for tumor therapy.
https://www.creative-biolabs.com/oncolytic-virus/introduction-and-mechanism-of-oncolytic-virus-therapy.htm
Introduction to SARS-CoV-2-Creative Biolabs
An ongoing outbreak of a novel coronavirus (SARS-CoV-2) has raised global concerns. It is identified as the cause of pneumonia with unknown etiology. Since the early outbreak in Wuhan, China, it has subsequently spread to all provinces of China and many other countries. The urgent epidemic situation has spurred the development of antiviral drugs and vaccines. As a leading service provider in the field of biological research and drug discovery, Creative Biolabs provides fast & elaborate therapeutic antibody discovery, drug candidates screening and vaccine development services to help combat this outbreak.
https://sars-cov-2.creative-biolabs.com/novel-coronavirus-sars-cov2.htm
Creative biolabs.cart nk cell-based-therapeutics
In addition to adoptive transfer, NK cells are also involved in molecular based therapies. Stimulatory cytokines, small molecule activators/inhibitors, monoclonal antibodies (mAbs) including bispecific antibodies (bsAbs), and antibody-drug conjugates (ADCs) are utilized to target inhibitory/activating receptors/ligands, tumor antigens, immuno-suppressive molecules, and the tumor icroenvironment, to improve the anti-tumor activity of NK cells.
https://www.creative-biolabs.com/bsab/
oncolytic virus immunotherapy
Oncolytic virus immunotherapy is a therapeutic approach to cancer treatment that utilizes native or genetically modified viruses that selectively replicate within tumor cells.
https://www.creative-biolabs.com/oncolytic-virus/
The development of oncolytic viruses
Oncolytic viruses have demonstrated selective replication and killing of tumor cells. Different types of oncolytic viruses including adenoviruses, herpes simplex viruses, Newcastle disease viruses, reovirus, coxsackie viruses, vaccinia viruses, etc., have been applied as either naturally occurring or engineered vectors.
https://www.creative-biolabs.com/oncolytic-virus/oncolytic-virus-basis.htm
Oncolytic virus immunotherapy
The strategy implementation of immunotherapeutic for the treatment of cancer has gained prominence over the past decade. Oncolytic viruses (OVs) are viral strains that can infect and kill malignant cells without harming normal cells, while simultaneously stimulating the immune system and creating antitumor immunity.
https://www.creative-biolabs.com/oncolytic-virus/oncolytic-virotherapy-development-for-combination-therapy-with-cancer-immunotherapy.htm
oncolytic herpes simplex virus
Oncolytic herpes simplex virus (oHSV) is one of oncolytic viruses being studied in cancer therapeutic research and several oHSVs have been investigated in clinical trials. ith the increasing experience and knowledge of HSV system, Creative Biolabs commits to developing efficacious oncolytic herpes simplex virus and enabling wider applications in oncolytic virotherapy of cancers.
https://www.creative-biolabs.com/oncolytic-virus/oncolytic-herpes-simplex-virus.htm
Immunogenicity manipulation
Nowadays, oncolytic virotherapy has gradually become a powerful immunotherapeutic modality for cancer treatment. Immunogenicity manipulation is quite necessary, and with genetic modifications, oncolytic viruses are able to improve the oncolytic effects on tumor cells and stimulate antitumor immunity.
https://www.creative-biolabs.com/oncolytic-virus/immunogenicity-manipulation.htm
Oncolytic virus basis
Oncolytic viruses (OVs) is a group of viruses that selectively replicate in and kill cancer cells, while leaving the normal tissue uninfected. OVs provide a diverse platform for immunotherapy; they act as in situ vaccines, and can be armed with immuno-modulatory transgenes or combined with other immunotherapies, such as immune-checkpoint inhibitor, engineered T cell, bi-specific antibody or chemotherapies.
https://www.creative-biolabs.com/oncolytic-virus/oncolytic-virus-therapy-development.htm
Exosome applications
Exosomes are nano-sized, membrane-enclosed vesicles released by nearly every cell type in the body. Exosomes contain various molecular constituents (genetic material and proteins) of their cell of origin and act as powerful messengers between cells, ultimately providing a regulatory function for many cell processes.
https://www.creative-biolabs.com/exosome/services.htm
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Exosomes Biogenesis&Therapeutics
- 2. 01 Evs Types and Characteristics Exosomes Biogenesis Roles in Normal Physiology and Disease Therapeutics & Drug Delivery Contents 02 03 04
- 3. Evs Types and Characteristics Extracellular vesicles: biology and emerging therapeutic opportunities[J]. Vesicle typs Characteristics Origin Size Marks Contents Exosomes Endolysosomal pathway; intraluminal budding of multivesicular bodies and fusion of multivesicular body with cell membrane 40-120 nm Tetraspanins (such as TSPAN29 and TSPAN30), ESCRT components, PDCDGIP, TSG101, flotillin, MFGE8 mRNA, microRNA (miRNA) and other non- coding RNAs; cytoplasmic and membrane proteins including receptors and major histocompatibility complex (MHC) molecules Microvesicles Cell surface; outward budding of cell membrane 50-1,000 nm Integrins, selectins, CD40 ligand mRNA, miRNA, non-coding RNAs, cytoplasmic proteins and membrane proteins, including receptors Apoptotic bodies Cell surface; outward blebbing of apoptotic cell membrane 500-2,000 nm Extensive amounts of phosphatidylserine Nuclear fractions, cell organelles
- 4. ILV Fuse with lysosome, which degrade multivesicular endosomes (MVE) contents. ① ② Fuse with plasma membrane where ILVs liberated into the extracellular space are released as exosomes. ① ② Exosomes Biogenesis
- 5. ① ② ③ ④ ⑤ Extracellular vesicles: biology and emerging therapeutic opportunities[J]. ESCRT-dependent ESCRT-independent (Ceramide-dependent) Small GTPases-dependent SNAREs-dependent Microvesicles formation ① ② ③ ④ ⑤ Exosomes Biogenesis
- 6. Roles in Normal Physiology and Disease Extracellular vesicles: biology and emerging therapeutic opportunities[J].
- 7. Immunotherapeutic Potential of Extracellular Vesicles [J]. Pro-inflamatory cytokine secretior (facilitate embryo implantation) Normal tissues Exosomes Intestine or milk Prostate or placenta Trophoblast Attenuate inflammatory response Inhibition of cytotoxicity (facilitate fertilization and pregnancy) Roles in Normal Physiology and Disease
- 8. Roles in Normal Physiology and Disease B cell Exosomes with MHC class ll-peptide complexes Primed CD4+ T cell Infected macrophage Exosomes DC B cell exosomes Macrophage exosomes 01 02
- 9. Mature /Immature / modified /pulsed DC Exosomes with MHC class l or ll-peptide complexes Primed T cell DC Naive CD8+ or CD4+ T cell Activation Antigen transfer Immunotherapeutic Potential of Extracellular Vesicles [J]. DC exosomes 03 Roles in Normal Physiology and Disease
- 10. Tumor cell Exosomes Exosomes with tumor antigens Tumor antigen- presenting DC Cytotoxic T lymphocyte Cytotoxic CD8+ T cell activation Inhibition of cytotoxic activity T cell apoptosis Immune suppression Induce differentiation ofmyeloid- derived supressor cells (MDSCs) Inhibition of DC differentiation Roles in Normal Physiology and Disease Immunotherapeuti c Potential of Extracellular Vesicles [J].
- 11. Exosomes as Therapeutic Targets ① ① Inhibiting extracellular vesicle production ② ② Inhibiting extracellular vesicle release ③ ③ Targeting extracellular vesicle components and inhibiting uptake Extracellular vesicles: biology and emerging therapeutic opportunities[J].
- 12. Exosomes as Therapeutic Targets Extracellular vesicles: biology and emerging therapeutic opportunities[J].
- 13. Exosomes for Drug Delivery Extracellular vesicles: biology and emerging therapeutic opportunities[J].
- 14. Creative Biolabs Services EXOSOME SAMPLING u Exosome Isolation u Exosome Purification u Exosome Characterization u Exosome Quantification u Exosome Profiling u Exosome Proteomics Services u Exosome Lipidomics & Metabolomics Services EXOSOME ANALYSIS EXOSOME MANUFACTURING u Exosome Engineering u Exosome Manufacturing EXOSOME-BASED APPLICATIONS u Exosome Antibody Development u Exosome Display
- 15. Web: www.creative-biolabs.com Email: info@creative-biolabs.com Tel: 1-631-381-2994 Address: 45-1 Ramsey Road, Shirley, NY 11967, USA Fax: 1-631-207-8356