Beyond the EU: DORA and NIS 2 Directive's Global Impact
Essay On Importance Of Peace. Essay On Industria
1. 💄Essay On Importance Of
Peace. Essay On Industria
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💄Essay On Importance Of Peace. Essay On Industria 💄Essay On Importance Of Peace. Essay On Industria
2. Applying Certificate Iv Of Training And Assessment
Applying Certificate IV in Training and Assessment to work in assisting youth towards market
demand in Timor Leste
Introduction
Timor Leste received independence on 30 August 1999 after a majority of the population voted for
pulling out of Indonesian occupation of the country. Life was not easy after getting the light of
independence as many sectors were destroyed to the ground by the militias. An enormous
responsibility rested on people to exert efforts to reorganize everything from the ground up. The
education system was highlighted as a core sector to be improved both in quality and service within
the country. Education and training are some of the main tools being used to wipe away the tears of
victims, who suffered much during the conflict. Technical vocational education and training (TVET) is
believed to be a key area which will assist in restoring many devastated sectors which totally
paralyzed the country. In early 2010, the TVET system was formally established in a national strategic
plan with a particular function in assuring, formulating and establishing the national qualification
framework (NQF) (PATVET, 2011). It has been tremendously effective through its system of
competence based training and competence based assessment in working towards market needs. This
TVET system has evolved smoothly in Timor Leste as it is culturally appropriate to local beliefs and is
flexible in its approach. The role of the TVET System in producing competent and qualified
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3. Fluorescence Spectroscopy And The Emission Wavelength Of...
Abstract Fluorescent properties are used to study protein and small molecule interactions. Fluorescent
spectrophotometry determined the excitation wavelength of eosin isothiocyanate to be 525 nm and its
emission wavelength was 545 nm. Glycogen phosphorylase was similarly studied. The excitation
wavelength was 330 nm and the emission wavelength was 360 nm. The emission wavelength could be
indicative of the presence of the fluorophore, tryptophan. The potential interaction between bovine
serum albumin (BSA) and 1 anilino 8 naphthalene sulfonic acid (ANS) was studied through
fluorescence and fluorescence resonance energy transfer (FRET). BSA had an emission wavelength of
358 nm which could also be indicative of a tryptophan. When BSA and ANS were mixed together, the
emission wavelength was longer suggesting that the molecules interact with each other and follow
FRET.
Introduction
Fluorescence spectroscopy is a useful technique to determine the proteins and nucleic acids of a
macromolecule. Some molecules, fluorophores, can absorb light at certain wavelengths and then emit
it at another wavelength. When the molecule absorbs light it is excitation and the light released is
emission. The light particles, photons, hit a fluorophore to excite the molecule (Voet et. al 2013).
Excitation occurs by the valence electrons of the molecule receiving energy and moving to a higher
energy state from the ground state. The excited electrons will later return to the ground state because
they
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4. Smackey Dog Foods
Introduction
Smackey Dog Foods, Inc. is a small, private company which specializes in manufacturing and selling
natural dog foods. Their new boutique division, Best Boy Gourmet has grown significantly due to
recent product demand. The company started small and is operated by sisters Sarah, Kim, and Jillian.
Sarah serves as the President and General Manager. Kim is the Production Operation Manager
overseeing the inventory, production, and shipment of the dog food with the help of her assistant,
Henry. Jillian is in charge of Sales with a team of 12 salesmen in Illinois, Indiana, and Wisconsin. The
business is based out of their hometown of Chicago. In an attempt to expand the business, Sarah has
applied for a loan of ... Show more content on Helpwriting.net ...
While making observations during field work, Ben and Maureen actually saw an employee take
previously thrown away dog food out of a dumpster and place it in Henry s vehicle. I knew from this
incident, that Smackey Dog Foods, Inc had a major weakness in their internal controls with inventory
and possibly an outright problem with theft. My team was present at the physical inventory count to
help ensure the accuracy of the count and we performed various analytical procedures on the
inventory such as comparing the gross margin percentage, the inventory turnover, and unit costs of
inventory with those of the previous years. We also ensured that all expired/obsolete inventories was
disposed of and not counted as we were aware of an issue that Smackey was having with the handling
of returned inventory. In order to confirm the accounts receivable balances, I decided to use positive
confirmations since this was my first time auditing the company and the collateral for the loan would
be the receivables. The confirmations helped to verify the accuracy and existence of the accounts. I
also calculated the Receivables Turnover Ratio in order to better evaluate the overall success of
collection on accounts. The sample size that I chose was determined by the factors of tolerable
misstatement, inherent risk, control risk, achieved detection risk
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5. Essay on Dred Scott v. Sandford
Dred Scott v. Sandford Dred Scott was born a slave in the state of Virginia around the 1800 s. Around
1833 he was purchased from his original owner, Peter Blow, by John Emerson, an officer in the
United States Army. Dr. Emerson took Dred Scott to the free state of Illinois to live, and under it s
constitution, he was eligible to be free. In around 1836, Dred Scott and his owner moved to Wisconsin
territory, a territory that was free under the Missouri compromise. It was in Wisconsin that Dred Scott
met and married Harriet Robinson. John Emerson was transferred in 1837 to Ft. Jessup, Louisiana,
were he met and married Irene Sandford. Dred Scott and his wife followed Dr. Emerson and his wife
from duty station to duty station; ... Show more content on Helpwriting.net ...
Sandford. Because Mr. Sandford resided in New York, there was diversity of residence, and the case
was taken to federal courts. Mr. Scott lost this suit as well, and this time appealed to the U.S Supreme
Court. The U.S. Supreme court ruled that Mr. Scott was a black man and therefore had no rights; this
included the right to sue. During the trial period, Mr. Scott and his family were in the custody of the
St. Louis county sheriff department. The Scotts were rented out and the money earned was held in
escrow for the winner in the case. Mr. Scott was financially backed by the family of his first owner,
and in fact Charles E Labeaume, the brother in law of Peter Blow, had been renting the family and
helped Mr. Scott sue for his freedom in the Federal Court. Dred Scott also sued Mr. Sandford for
battery and wrongful imprisonment. Mr. Sandford did not deny Mr. Scotts claim, he agreed that he had
gently laid his hands upon Scott and his family, but that he could do so because they were legally his
property. Sanford won this case because Mr. Scott s status as a slave had already been decided by
Missouri Supreme Court. There was also the question that when Mr. Scott did reside in the free states,
why didn t he claim his freedom then? In the past Missouri courts supported the doctrine of once free,
always free, however Dred Scott could not read or write. Money ran low for the Blow family and they
were no
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6. The American Dream Of Home Ownership
Too many Americans have fallen victim to the crisis that has become the norm for our citizens these
days. Lenders no longer want to work with individuals who have gone through the foreclosure process
and for many it is not only their homes they lose. Some have lost their jobs and/or families, others fall
into a deep depression and worst of all some have taken their own lives. An alternative would be for
lenders to add a true customer service department which is not based on pay or get out, but is
counseling based to keep Americans in the American Dream of home ownership. This would not only
keep their customers for future business with new home loans, but would be noticed by neighbors and
the community who are appreciative their home values are not decreasing. The lenders would counsel
them through the sale by assisting them with getting the most they can for their home. By getting local
community organizations involved to help with home repairs and curb appeal. Once the home is
market ready and a dollar amount has been reached for these repairs the home owner will have to
volunteer time and/or resources to assist another person going through the same situation. This would
not only help the homeowner but will keep homes in the area selling at a fair market value and not
bring down the values in their neighborhood. The assistance provided by the lenders and assistance
from the community will help build their self confidence and make them feel like they are not alone.
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7. Business Cycle And Recovery Period Analysis
Introduction The period following a recession, where the GDP of the economy is increasing, is
encapsulated in the term recovery . Subsequently, an increase in national output would ensue, thus
prompting the recovery and growth of the economy. As opposed to the previously existent
contractionary period, the economy would now be working at higher capacity allowing factors such as
the rate of unemployment to decline, in addition to increasing productivity. Illustrated in the diagram
below shows the business cycle and recovery period. As shown in the diagram, the recovery stage
occurs between the trough (the lowest point in the graph, and where the real output is equal to the
potential output. Impact on values of imports The term ... Show more content on Helpwriting.net ...
Nevertheless, despite a decline in quantity demand for these good and services, the resultant increase
in price may balance the decline that occurred to the demand. Thus, as a result of the elasticity of price
of imported goods, the increase of price affecting imports, caused by deprecation of currency, may
ultimately have negligible effect on the value of imports, and might possibly even cause it to increase.
Additionally, despite the depreciation of the British pound in 2007, the UK s economy experienced a
3.1% increase in imports. This is a result of the UK economy being at a stage of recovery, which
would cause to increase imports, as the manufacturing division would import an increased amount of
foreign products. Thus, it is plausible that a fall in the value of the GBP, which would inevitably cause
an increase in price of imports, may prompt the value of imported goods and services to remain
stagnant or decrease. Due to decreased labour productivity In the instance of a decline in the labour
productivity of the UK in its entirety, a decline will consequently occur in the general production of
goods in the country. This would trigger a chain of events that would concomitantly increase the cost
of production per unit for the producers, causing the supply to decrease, regardless of the price it is
sold at. These circumstances would result in a reduction of the aggregate supply (AS) of the
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8. This is a speech for a US Representative running for...
First off I want to say that I am very happy to see all of you here. I am going to use this time to
announce my candidacy for Senate. After much deliberation I have decided to leave the House of
Representatives after my three terms and run for a position in the United States Senate. I believe that I
am the right candidate for this position for several reasons. First of all, I was in the House for six
years, because of this I understand how the institution is run from the inside, and I understand the
problems of the Senate. I believe that this is a very important aspect when running for the Senate.
Second I realize that Congress has some fundamental problems, that come from individual goals as
well as institutional goals, and I want to take ... Show more content on Helpwriting.net ...
As you can see from these examples though this problem is not just unique to Congress. It can also be
seen in the state legislatures of the colonial era, and in the British House of Commons of the sixteenth
and twentieth centuries
I believe that there are ways to make Congress better by solving these problems. Three proposals that I
believe will help alleviate the problems we are having with Congress is campaign finance reform,
more deliberation in Congress and public education of issues.
Campaign finance reform is probably one proposal that you might be familiar with and it is my belief
that implementing this will help improve Congress as an institution. There are several areas of this
proposal that I find important. These issues include the elimination of soft money, raising the limits on
party contributions, and encouraging more participation of the public in campaigns.
Soft money is not being used for the same reasons as it was originally meant to be. What originally
started out to help grassroots party activities has turned into a cover for the contribution of political
parties to donate large sums of money, which can be used as a way to influence the vote of certain
government officials. In 1998 the Republic Congressional Campaign Committee held a dinner that
raised 10 million dollars and since 1995 Democratic Party committees have been receiving over 1
million dollars
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9. Diabetic Teaching Plan for Nurses Essay
C.S. is a morbidly obese 32 year old female admitted to the hospital on 8/26/05 with an admitting
diagnosis of poly drug overdose. According to the patient, the last thing she remembers is going to bed
and then waking up in the hospital 2 days later without any recollection of what had happened. She
has a history of suicide twice in the past, but denies suicidal ideations this time. C.S. also has a history
of Diabetes type II that is normally controlled with oral medications. She states that she checks her
blood glucose at home and it has been in the 200mg/dl range for the past month. Current blood
glucose is 256mg/dl. C.S. is to be discharged tomorrow with a new prescription for insulin.
Assessment data that indicates learning ... Show more content on Helpwriting.net ...
Specific learning objectives:
Learning objective #1: (cognitive) patient will be able to verbalize the signs and symptoms of
hyperglycemia and hypoglycemia and the actions to take with each situation.
Learning objective #2: (Affective) patient will be able to verbalize the benefits of maintaining
acceptable blood glucose levels and the importance of taking insulin exactly as prescribed.
Learning objective #3: (Psychomotor) patient will demonstrate ability to self administer Insulin with
little, or no prompts. The estimated time for the teaching session is approximately 3 hours. In the first
hour I will give a brief overview of what diabetes is and how it can be controlled with insulin. First I
will include a very simple explanation of the pathophysiology of the disease and the signs and
symptoms that are common. Next, I will explain that when controlling diabetes, her blood sugar can
become too high or too low. High blood sugar, or hyperglycemia is caused by eating too much food,
eating sugary foods, or by not taking insulin as prescribed. Signs of high blood sugar include: dry
mouth, thirsty, having to urinate often, blurry vision, feeling tired, and weight loss. If high blood sugar
is not treated, it can cause you to go into a coma (Aldridge, 2005 p.36 37). When you have high blood
sugar, drink water and take your insulin according to the sliding scale your doctor has provided. Low
blood sugar, or hypoglycemia, can occur
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10. The Accounting Principles Of Financial Statements And...
Accounting in health care has profound effects on financial statements, and knowing the necessary
components to conducting financial reports and balancing the assets is vital. Many things contribute to
the overall foundation of the balance sheet and valuations for the health care organization. A lot of
moving parts are required to keep health care organizations running and afloat for the long term. One
must understand the accounting concepts, and methods, terminology, in order to explain the various
uses on how they apply to balance sheets. Valuing assets and Generally Accepted Accounting
Principles (GAAP) method is effective for the right valuation as they will be explained in the
following paragraphs.
Differences between Cash and Accrual Accounting
Cash accounting is a simple method which deals with revenue and expense within the health care
organization and personal uses. This method can easily apply to everyday people as several people
have a personal checkbook; they use to keep track of their expenses and depositing money every so
often. Using a checkbook is how we withdraw money for expenses which is one standard way to pay
for items. Cash basis accounting is also applied when reporting salaries for physicians or registered
nurses as an expense and this would allow no revenue of patients treated by physicians until the
following year when profits are collected. As good as method this is for people, accountants strive for
a better way to assess the organizations
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11. A Career In Pottery
intro
To be a successful potter in today s society, one needs to be innovative, creative, and people smart.
They need to be able to read a crowd and their desires. Many popular potters have creative minds,
creating new and innovative works on the spot. If you are a self employed potter, you need to be
business savvy in order to survive in today s economy. There will always be a consumer market for
pottery, but one needs to know where to look. According to local potter Ken Tracey, disipline and
motivation are indispensable skills for potters (5). The U.S. Bureau of Labor Statistics shows the
pottery profession has an expected job growth rate of 3 percent within the next 10 years (U.S. Bureau
of Labor Statistics 6). However, Learn.org suggests an expected rate between 8 and 14 percent for all
potters in ... Show more content on Helpwriting.net ...
Whether one is a potter as a full time or part time job, both regions are expected to grow ( Careers in
Pottery 1). The pottery industry is not an individual industry it s success and decline can, and does,
affect other businesses. When a potter rents or builds a studio, the building s owner or contractor gains
business from the potter. If a property owner is renting a building to a potter, and the potter goes
bankrupt, the property owner is affected, also. Manufacturing is a large part of the pottery industry,
along with self employment. Mass produced pieces are very popular, being sold at retail stores all over
the world. According to O Net OnLine, the top industries are manufacturing and self employment (
Potters, Manufacturing 16). If a prevalent potter has their work featured at a gallery or museum, the
museum and the surrounding area receive an influx of business from the fans of said potter. When
people travel and pay to see the work, they may rent a room at a nearby hotel or motel, they eat at
nearby restaurants, shop at nearby stores, among other numerous actions. Pottery is not a stand alon
industry its success may affect the surrounding areas
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12. Eldorado Comparison
Similar to the HG Wells story, in the poem Eldorado by Edgar Allen Poe the romance genre is
demonstrated through a place apart, and also demonstrates a serpent in the garden. This is
demonstrated through the knight and the shadow trying to find Eldorado; a place/county that was
known for having the most gold during the gold rush in 1848. In the first stanza of the poem
everything seems perfect Gaily bedight, A gallant knight, In sunshine and in shadow , although still
when the second stanza starts the knight is in the same optimistic state; that he will eventually find
Eldorado and prosper. There are still glimpses of a gloomy future, the knight is old, a shadow is
growing over his heart, and he is failing his quest. The shadow that was originally
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13. Quality Of Measures
This paper will highlight the information epidemiologists utilize to evaluate the quality of measures
used in research as well as provide reasons why the quality of measures are essential and finally
explain how epidemiologists ensure the measures used in research are of good quality.
a. What type of information do epidemiologists use to judge/evaluate the quality of their measures?
Tests for validity and reliability are the criteria mostly used by epidemiologists to judge the quality of
measures used in research. Reliability of the measure shows that when different observers use the
measure at different occasions, they will obtain similar test results (1). For example, a measure that
determines weight gain among male adults in Saskatoon ... Show more content on Helpwriting.net ...
The standards of acceptable reliability which epidemiologists seeks to attain include internal
consistency exceeding 0.8 and stability measures greater than 0.5 (3), nevertheless, even if the internal
consistency of a measure is low, it is better the measure has high stability and content validity.
Validity tests involve ensuring that the instrument is measuring what the epidemiologist intended (4),
validity has several components including content and construct validity. Validity of the measure at
minimum can be assessed subjectively by expert opinion of one or more experts in the field of study,
where experts review the measure to determine if the instrument appears to be assessing the desired
qualities (Face Validity) or determining whether the instrument samples all the relevant content
(Content Validity) (3).
Additionally, constructs are mini theories used to assist in developing a new or better instrument in
order to explain a broader range of findings or make more accurate predictions about a person s
behavior. Hence, when no other measures exist, the epidemiologist can link an attribute measured to
another attribute by a hypothesis or construct and test the instrument by applying it to a sample to
determine if the expected relationship is obtained; this is regarded as construct validity. For example;
if a test for blood sugar was devised because there is no other test
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14. Hysterectomy In Surgery
New Surgical Technique has Dangerous Side Affects
Over half a million women will undergo hysterectomies this year [according to whom?]. Many of
these surgeries are performed to remove fibroids, as well. These benign growths in the uterus typically
cause pain, bleeding, and other symptoms. There are several techniques used by medical professionals
to perform a hysterectomy and to remove fibroid tumors, but one the power morcellator has raised
serious cause for concern.
The Power Morcellator
The power morcellator is a device that has been used in laparoscopic surgeries over the last 10 years
or so to remove fibroids, the uterus, or both. This procedure is less invasive than an open
hysterectomy with a shorter recovery time for patients, ... Show more content on Helpwriting.net ...
Because it is more invasive, it is attached to six weeks of recovery and a large scar. There is also an
increased rate of complications from blood clots and infections with this surgery.
Vaginal Hysterectomy
This procedure uses the technology of the laparoscope to cut away the uterus, which is then removed
through the vaginal canal. The morcellator is NOT part of this technique. Because there are minimal
incisions, the recovery time is dropped to approximately two weeks. There is decreased rate of clotting
or infection, and 20% of hysterectomies are currently being performed using this technique. This
procedure is not utilized if the patient has an enlarged uterus or extremely large fibroids.
Other Techniques
There are some additional techniques that have been used to rid the uterus of fibroids including
ultrasound surgery and embolization. The ultrasound surgery uses sound waves to heat and destroy
fibroids, while embolization cuts off the blood supply to the fibroids, causing them to shrink and die.
If you are evaluating the different procedures for a hysterectomy or fibroid removal, we encourage
you to carefully consider your options and the risks
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15. Taxation Law
TAXATION LAW
WRITTEN GROUP ASSIGNMENT
PART A
ISSUE 1:
Is Andy a resident of Australia for the purpose of income tax?
RULE:
S6 (1) ITAA 36 definition of resident
There are four alternative tests helping to decide whether a person is a resident of Australia or not. An
individual needs to satisfy at least one test to prove residency.
(1) Common Law test; (2) Domicile test; (3) 183 day test; (4) Superannuation test
APPLICATION:
(1) Common Law test
According to ordinary concepts of resident , an individual should satisfy the Common Law test . In
this particular case, Andy spends the last three years working in Thailand, having signed a contract
with a Canadian company in Australia, which doesn t fulfill ... Show more content on Helpwriting.net
...
(3) 183 day test
The 183 day test is satisfied when a person who has actually been in Australia, continuously or
intermittently, during more than one half of the year of income.
In the given case, Andy spent most of the year working in Thailand and he only got back to meet his
family in Australia one month every three months. Therefore, he does not satisfy this test.
(4) Superannuation test
As there is no sufficient information regarding Andy s superannuation, the test is not applicable here.
16. CONCLUSION:
As can be concluded by the above test and analysis, Andy satisfies the Common Law test and
Domicile test. Even though the 183 day test is not satisfied and the superannuation test is not
applicable, we can still be sure that Andy can be considered as a resident for the purpose of income tax
assessment.
ISSUE 2:
How should Andy s salary and investment income be taxed?
RULE:
S6 5 (2) if you are an Australian resident, your assessable income includes the ordinary income you
derived directly or indirectly from all sources, whether in or out of Australia, during the income year.
S6 10 (4) if you are an Australian resident, your assessable income includes your statutory income
from all sources, whether in or out of Australia.
S4 1 and S4 10 ITAA97:
Income tax = (taxable income * rate) tax offset
Where taxable income = assessable income deductions
APPLICATION:
Both the income of
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17. Miss Julie Character Analysis
Our attitude towards what is normal in terms of gender as well as social class has changed
dramatically from the Victorian Era into the Modern Era. During the Victorian Era, women had clearly
defined roles as housewives, mothers, caretakers, and mediators, however, this all began to change
during the modern era as the ideology and morals shifted over time and women began to take on a
more flexible and dynamic role. Through the presentation of Julie and Jean s nontraditional characters
in the play Miss Julie, August Strindberg explores the themes of class and gender dynamic during the
late Victorian era to be one that is shifting. The 1889 play took place in an elaborately decorated estate
that is home to a Count and his daughter, Miss Julie. Miss Julie s engagement recently ended and she
finds herself in the estate kitchen one evening and has an affair with one of the servants, Jean, who is
engaged to another servant, Christine. Julie and Jean end up dancing together before they disappear to
Jean s bedroom, and then making plans to run away and begin a hotel business together. They discuss
the harsh realities from their childhood and reveal secrets to each other over the course of their night
together. Jean talks about growing up poor and how he would admire Miss Julie when she was little.
Miss Julie discusses the trouble between her mother and father that brought along her hatred of men.
After the two bond, Christine wakes up and Jean tells her what happened between him
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18. Fairport Animal Hospital Case Study
Your pet s health should never be compromised. When looking for the right animal hospital, you
should expect top notch care of your pet as well as great customer service. Fairport Animal Hospital, a
veterinary facility in Fairport, NY, has the right equipment and employs the best staff in the area so
you can be sure that your animal companion is in good hands. Whether it s your first time to take your
pets to an animal hospital or you re looking for a new facility in your area, it s essential to choose a
hospital that can adequately address your pet s needs.
What should you expect from a good animal hospital?
Specializes In Animal Surgery: A good animal hospital can perform various surgical operations on a
number of different species of animals. Fairport Animal Hospital performs spay and neuter
procedures, tumor removals, caesarean sections as well as reconstructive surgery. They can also
perform surgical operations on avian, reptile and other small mammals. ... Show more content on
Helpwriting.net ...
Your veterinarian should be patient enough to explain to you the condition of your pet and the kind of
procedures they will have to undergo. Sometimes your vet will suggest running exams such as blood
and urine test to diagnose your pet s health problems.
State Of The Art Facilities: The level of care an animal hospital is able to give your pet will be limited
by their equipment and laboratory facilities. Fairport Animal Hospital houses modern hospital
equipment that allows vets to give your pet the best medical care possible. They have digital
radiology, ultrasonic imaging, ECG s, and CO2 Laser. Pet microchips are also available in their
... Get more on HelpWriting.net ...
19. Egg Observation Paper
Samples preparation and observation of egg activation and cell division The gametes used are from
the species Strongylocentrotus purpuratus. Sea water containing unfertilized eggs was collected and
four experimental conditions were applied. Before applying the treatments to the eggs, sea water was
incubated in 16ºC water for 10 minutes. In the first experimental condition, 1mL of seawater was
collected as a negative control. In the second experimental condition, 1µL of sperm was added to 1mL
of sea water, as a positive control. The third condition contains 1mL sea water and 5µL of Ca2+
ionophore (A23187) prepared as a 1mM stock in DMSO. A23187 is a hydrophobic compound
responsible to facilitate the diffusion of Ca2+ from the endoplasmic reticulum to the cytosol. DMSO
is a substance used to add hydrophobic compounds to cells, since it is miscible in hydrophilic and
hydrophobic solutions. The last condition contained 1mL of sea water and 5µL of DMSO, which
works as a ... Show more content on Helpwriting.net ...
Then, the samples were incubated for 35 minutes in 16ºC water bath, the eggs were analyzed under
low magnification microscope and scored for egg activation. 1mL of each sample was used to prepare
western blot samples, which were centrifuged for 30 seconds at full speed at a micro centrifuge.
MAPK lysis buffer (1% NP 40, 20mM HEPES pH 7, 15mM EGTA and 150mM NaCl) combined with
10x inhibitor cocktail (2mM Pefablock, 10µg/mL Pepstatin, 100mM ß glycerophosphate, 4mM NaF
and 2mM Na3VO4) was prepared, and 48µL of this solution was added to the egg pellets to
homogenize them. The samples were centrifuged in a cold room centrifuge at full speed for 10
minutes. The supernatant was retrieved from each tube and 5X SDS PAGE buffer was added. The
samples were heated at 95ºC for 2 minutes and stored in the
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20. The Film Moonlight By Barry Jenkins
It s more than difficult living in a drug plagued, impoverished community. These places are often
ignored, but just as important as anywhere else. The film Moonlight, directed by Barry Jenkins, is
meant to demonstrate the hardships of living in a difficult community. Thematically, it highlights the
already difficult nature of being black in modern America, while simultaneously showing the hardship
of also being gay in said tough community. The film is comprised into three acts surrounding the
development of the protagonist, Chiron (in order of acts: Alex R. Hibbert, Ashton Sanders, and
Trevant Rhodes). Following him as he struggles with his own sexuality. Consequentially, each act
circles back to Chiron at a local beach, which for him, is ... Show more content on Helpwriting.net ...
The blocking in this scene is also a foreshadow for the rest of the movie. In the beginning, Chiron is
scared and unsure of who he is, turning to the help of others like Juan and his girlfriend, Teresa.
However, after trial and tribulation, Chiron learns how to swim on his own. He slowly starts
understanding how to cope with his sexuality even if he s not accepted. And unfortunately, for most of
the film, Chiron is subjected to the sad realities that engulf everyone around him.
As expected, this happy scene is cut short as Chiron is dropped off at home to find his mother with
drugs and a stranger in the house. The scene is meant to immediately draw the viewer back to the real
world, the one Chiron has grown up in. In actuality, Chiron can t just escape the situation he was born
into. The mise èn scene used in this moment is meant to demonstrate how the mother is quickly losing
grasp of a happy life; trying to escape their reality by any means necessary. The blocking of Paula
portrays her as anxious and angry, grabbing what appears to be drugs from her dining table. The
lighting is also dark and the house is dirty, reinforcing the idea that her life is slipping away. This is
just one of many situations in which Chiron is snapped back into reality after feeling accepting for
who he is.
Act two cuts to Chiron as a high schooler. Like before, his quiet nature seems to get the best
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21. Essay about We Wear the Mask
Paul Laurence Dunbar, dispatches the cold troubles of African Americans in the lyrical poem, We
Wear the Mask. In this poem, Dunbar links imagery, rhythm, rhyme, and word choice to in order to
institute a connection to the reader. From reading the poem, one can infer that Mr. Dunbar is speaking
in general, of the misery that many people keep concealed under a grin that they wear very well. But if
one were to go further and take the time to research Mr. Dunbar s selection of this piece and the era of
which this poem was written, one would come to understand that this poem focuses entirely on Paul
Laurence Dunbar s viewpoints on racial prejudice and the struggle for equality for the African
American s of his time period. Though this ... Show more content on Helpwriting.net ...
Since it does, when reading each line, there is a resilient connection that allows the reader to put
together and feel for what the narrator is speaking of. As each line is metrically linked, the words are
further recited in a durable voice and the poem is virtually put together, musically. In the first and
second lines of the third stanza, an apostrophe, a figure of speech that directly addresses an absent
person or entity, is presented, We smile, but O great Christ, our cries to thee from tortured souls arise.
Alliteration and metaphors are two major elements of this poem. The repetition of consonant sounds
and alliteration occurs throughout the poem. All of the lines strongly use the repetition of consonants
and alliteration except for lines two and seven. In addition to that, the controlling figure of speech in
this poem is a metaphor. It is strongly articulated in the first line, and as the one continues to read, it is
amplified and extended throughout the rest of the poem. The metaphor compares mask of Line 1 to the
fabricated emotive facades that African Americans had made use of in order to avert provoking their
oppressors.
Ultimately, since Dunbar avoids specifically mentioning blacks and their suffering, with the history of
this poem in mind, this poem could stand as a lament on behalf of all of the individuals who were
forcefully made to wear a mask just as a girl who tries to hide her pregnancy from her parents, or as a
boy who
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22. Greek Influence On The Modern World
The Greek influence in the modern world is still significant today. In a matter of words Hellenism
brought us intelligence. The cultural significance of Hellenism in this modern world brought us
libraries, museums, art, architecture, language, warfare tactics and weapons, philosophers and
philosophies, and democratic societies. Hellenism in the modern world means to adopt Greek culture
even in non Greek regions. Hellenism has brought to the modern world many different things that may
have been once impossible. Hellenism has brought literature comedies epic tales, poetry, and tragedy.
In modern terms this is converted into books, movies, theatre, television, plays, and they have become
a part of everyday life. Furthermore, Hellenism has brought us philosophy, which in a way increased
intelligence. With the likes of Pluto, he brought us ethics, justice, and methods of acquiring
knowledge. Aristotle brought us ... Show more content on Helpwriting.net ...
Thales of Maletus, studied astronomy and was the first to predict an eclipse of the sun. He also
calculated the height and distance of things, and was considered an inventor and engineer who is also
interested in electrical properties. Democritus believed that the universe contained many different
worlds which may be inhabited. He researched different things, such as minerals and plants, he was
also interested in mathematics and geometry.
The Greeks had a love of architecture, and as such they strove for perfection. They were known for the
temple designs, however they also built theatres for entertainment, stadiums for sporting events, and
they paved roads. In Alexandria they built the Pharos lighthouse, they also built a great library, and
museum, so architecture was very important to the Greeks and today it is important to
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23. Habeaus Corpas
The War against Terror as War against the Constitution Jackson A. Niday, II Abstract: This essay
examines rhetorical dynamics in the 2004 US Supreme Court case Hamdi v. Rumsfeld. News reports
suggested the court split 8 1 or 6 3. However, case texts show substantive disagreements created a 4 2
2 1 split in the court. Moreover, while the justices on the bench split into four camps rather than two,
those camps were not defined along ideological lines. This essay argues that pragmatism, the legal
philosophy that held sway in the case, achieved practical expediency at the expense of judicial and
constitutional coherency. In the end, the court found a majority through neither persuasion nor
principled conviction but, rather, through ... Show more content on Helpwriting.net ...
Each of these philosophies has engendered rich schools of thought and many tomes have been devoted
to defining each position, but we can distill working definitions from their governing hermeneutic
principles. Textualism (often called strict constructivism) calls for an interpretation and application of
the law guided by taking the words of a source document literally with strict adherence to etymology
and historical precedent.1 Legal pragmatism, in contrast, adopts a hermeneutics stressing (1) the
importance of context; (2) the lack of foundations; (3) the instrumental nature of law; and (4) the
unavoidable presence of alternate perspectives. 2 In the Hamdi case, Justice O Connor s pragmatism
battled and bested Justice Scalia s textualism. That triumph, however, was a narrow victory, and a
victory riddled with dissonance. It was also a victory that evoked from Scalia one of the more scathing
jeremiads he has issued from the bench. In what follows, I shall analyze the argumentation of Hamdi
in terms of substance and style, and will look at the various opinions issued, along with a detailed
examination of Scalia s lamentation. A decidedly American philosophy, pragmatism seeks what works.
In that pursuit, its focus on the instrumental often privileges expediency over principle. For many
human endeavors, finding expedient resolutions simply
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24. Review Of James Fenimore Cooper s The Last Of The Mohicans
Portrayed in the midst of the French and Indian war, The Last of the Mohicans, by James Fenimore
Cooper, focuses on life in the frontier and the violent clashes the French and Native Americans
experience with the English during the French and Indian war. Hawkeye, a white hunter who
identified closely to the Native Americans, becomes entangled in the grotesques battles happening in
the French and Indian war in order to save the lives of two women. Although written seventy five
years past the prime of the events happening in the novel, Cooper implements and manipulates factual
events in history to provide a subtle idea of the conflicts that happened in the French and Indian War.
France and Great Britain went to war during 1754 due to the increased tension of territorial expansion.
It is true that Major General Webb abandoned fort William Henry and appointed Lieutenant Munro
who was unprepared for the anticipated French attack. ... Show more content on Helpwriting.net ...
The Indians in this romantic adventure novel are helping their allies for this same reason. Tribes who
may have not felt any maliciousness towards each other were made to due the tension of the war.
Cooper captured this through the Delawares who were made to initially oppose Hawkeye and his
friends. The title of this novel foreshadows the genocide of Indians throughout the course of the war.
The survivors of the Mohican tribe are Uncas and his father Chingachgook. In chapter 33, Uncas dies
in an attempt to save the life of his beloved Cora, the fictitious daughter of Munro. Tamenund, the
leader of the Delaware tribe says, The pale faces are masters of the earth. And the time of the red men
has not yet come again (Chapter 33). His optimism of the Native American people acquiring the
opportunity to gain power is ironic because the Indians do not gain any power in reality; instead they
are forced off their
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25. The Pros And Cons Of Rudolf Diesel
In the year 1885 Rudolf Diesel sets up a small shop in Paris to begin developing an engine that would
forever change the way we look at transportation. Rudolf was trying to design an engine to run off
compression ignition to replace the costly steam locomotive. His research lasted around 13 years
before showing a successful test of a slow burning, efficient, compression ignition engine. diesels had
his first running engine built on February 17,1897 with an efficiency of 26.7% v.s the steam engine s
efficiency at 10%.
Rudolf had paved the path for modern diesel engines, quickly evolving from running on coal dust to
having computerized injectors with injection so microscopic water is incapable of traveling through in
a little over a century. ... Show more content on Helpwriting.net ...
One pro of diesel engines is also a con. While a diesel engine is an internal combustion engine it fires
off of the heat of the engine. The heat is caused from the friction inside the cylinders, this causes the
engine to have a little less maintenance and run more efficiently. The con of diesel engines combustion
off of cylinder heat is in northern climates it isn t always warm enough for the engine to maintain
enough heat to run. They solved this problem in the northern climates by installing glow plugs and
block warmers. Glow plugs are used to heat the fuel before it gets injected into the cylinder helping
the motor start because the heat needed for combustion isn t used to warm the fuel. Block warmers are
a small heater that is placed in a soft plug hole on the side of the engine and helps keep the coolant
warm so that the block stays warm while the truck is sitting for a week or a few minutes. Cold weather
can cause more then just starting issues for diesel engines it can cause them to just shut off from a lack
of fuel, the diesel itself will gel in cold weather and be like a jello like substance and then needs
additives added to the fuel to be bring it back to its original
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26. Margaret Mead
Margaret Mead (1901 1978)
Margaret Mead was born on Monday, December 16, 1901, at the West Park Hospital in Philadelphia,
P.A. Margaret was the first baby to be born in this hospital, and because of this, she felt different from
the rest of the children, because they had all been born at home. Margaret #8217;s parents were from
the midwest, and because of their professions, the family moved quite a bit living in such places as
Hampton, New Jersey; Greenwich Village in New York City, and St. Marks Square in Philadelphia.
Because she moved so much as a child, Margaret had been subjected to many different styles of
living, and therefore had a growing desire to learn more about different lifestyles and cultures. ...
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In 1949, she was president of the society of applied anthropology. In 1942, she was awarded a gold
metal from the society for women geographers. Margaret Mead taught generations of Americans about
the value of looking carefully and openly at other cultures to better understand the complexities of
being human. Margaret died in 1978 having lived a very enriched life. Margaret Mead was a clean and
forceful person, who had a great impact on the world of psychology and anthropology, and to her we
owe much knowledge. Her work has, and will continue to impact the daily lives of people around the
world. Her 44 books, and more than 1,000 articles have been translated into almost every language.
Her data has been carefully catalogued and
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27. Holden s Alienation In Catcher In The Rye
Holden never quite fitted in to society s tradition norms and this is obvious from his poor academic
performance, scoffing at authority and his distinct choice of clothing that is promotes his isolation
even more but what exactly is Holden alienated from? At best society, but more importantly himself
and others and since Holden does not own up to his own mistakes nor ever assess his judgment which
purely reflects himself, he remains alienated from the world. Early one we learn that Holden is failing
the majority of his classes and the closest person to being considered his friend is Holden s history
teacher: Mr. Spencer, who is only someone who feels responsible for Holden s negligence in academic
performance but this is nothing extraordinary since Holden convinces many others to feel for him. ...
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Haas and his friend Stradlater for being unscrupulous while comparably, Holden isn t any different.
For instance after agreeing with Old Spencer regarding life as a game, internally Holden admitted that
this idea was idiotic and Holden had no problem divulging this to the reader and the reader might find
this insignificant but quite coincidentally, these occurrences happen whenever reality is too much for
Holden to handle. A great example of this was when Old Spencer asked Holden if his future was
significant to him, to which Holden divulges to the reader that this subject is quite depressing for him
but in order for a simple concept such as the future to be so depressing, one may infer that Holden has
gone through a series of unhealthy events in his life that have made him feel as if life is
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28. Short Note On Procedural Memory
Long Term Memory
Procedural Memory Apart of the long term memory, responsible for knowing how to do things ex how
to ride a bike
Procedural Knowledge Knowing how to simple things ex how to tie your shoe or riding a bike, this
does not include conscious thoughts (conscious aware, so not aware of doing actions.
Semantic Memory Knowledge of meanings, the basics and about the world
Episodic Memory Stores info from events that we have experienced
Endel Tulving An Estonian Canadian experimental psychologist and human research on memory
influenced psychologist, scientist, and others. He defined long term memory(one of the earliest to
define long term memory). He proposed to define the difference between episodic, semantic, and
procedural memory.
Declarative Knowledge Knowing, ex when mom s birthday is, dogs are animals, and Columbus is the
capital of Ohio. Long term memory is located in the inner fold of the temporal lobe. Temporal lobe
lies beneath the temples on the head. The temporal lobe controls hearing.
Alzheimer s
Alzheimer s A disease in the brain that destroys memory and other functions.
Symptoms
Cognitive: Confusion,difficulty thinking/understanding/concentrating, makes things up and not able to
create new memories,can t do simple math or not able to recognize simple things.
Behavioral: aggression, agitation, difficulty with self care, irritability, meaningless repetition of own
words, personality changes, lack of restraint, or
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29. The British In Battle-Personal Narrative
Today we finally beat the British in battle, it was an amazing victory, although i must say, I really miss
my long hair. Its a lot harder than I thought it would be to hide that I m a girl, I cant go to the
bathroom, and getting dressed is complete hell. I remember being called out of my deep slumber after
Paul Rever saw the British coming. As we were all standing waiting for the British soldiers at around
five a.m. I heard the shouting, The lobster backs are down the road! Led by Major John Pitcairn, the
British troops approached Lexington. As we were lined up, preparing for battle, we were told to not
fire unless we were fired upon. Our leader Captain Parker called for us to back down after seeing how
many soldiers the British had and
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30. Essay Flannery O Connor s Good Country People
Flannery O Connor s Good Country People
In Good Country People by Flannery O Connor, uses symbolism in the choice of names, almost to the
point of being ironic and humorous.
These names center around the personality and demeanor of the characters.
Hulga, once known as Joy, simply changed her name because it was the ugliest she could think of.
Mrs. Freeman s name is ironic because she is burdened by the land that she works, so is not really
free. Mrs. Hopewell?s name is also ironic, because she trys to provide hope, but is in fact empty in her
talk. Each one of these characters names, Hulga, Mrs. Freeman, and
Mrs. Hopewell, show the symbolism used by Flannery O Connor.
Hulga, the daughter to Mrs. Hopewell, was actually ... Show more content on Helpwriting.net ...
She does not think of herself as pretty, but in fact she believes that she is ugly and depressed and
actually enjoys feeling this way.
Mrs. Freeman is the tenet farmer for Mrs. Hopewell. Mrs. Freeman is very nosy and has a fondness
for the grotesque and secret infections. She has an attitude of trying to be someone she is not, almost
as if she is trying to be like Mrs. Hopewell. When Mrs. Hopewell stated to Mrs. Freeman one of her
many phrases ?that life was like that, Mrs. Freeman would say, ?I always said so myself.? Nothing had
been arrived at by anyone that had not first been arrived at by herself.? Mrs. Freeman?s name could
mean two things. One possible meaning is that a freed slave was called a freeman, but
Mrs. Freeman is a white tenet farmer, so technically she is not free. This could show how Mrs.
Freeman would rather be a freed black slave, rather than a poor white farmer tied to the land. The
other meaning is that when people first came to America, many people would become indentured
servants, and then over time they would eventually get their freedom. These people were called
freeman. This meaning could show how Mrs. Freeman had a desire to be free and not work for
someone else. One day Mrs.
Hopewell had said to Mrs. Freeman, ?You know, you?re the wheel behind the wheel,? and winked,
Mrs. Freeman had said, ?I know it. I?ve always been quick.? This shows that perhaps Mrs.
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31. Critical Speech In Perils Of Indifference By Elie Wiesel
Romanian born American Jewish writer, professor, political activist, Nobel Laureate, and Holocaust
survivor, Elie Wiesel in his critical speech, Perils of Indifference, maintains the idea that indifference
is inhumane. He supports his claim by using his personal experience in the Holocaust. Additionally, it
states in paragraph 9 that They were dead and did not know it. emphasizes how indifference has made
the Muselmann completely unaware of their fate. Further, it states in paragraph 8 that Indifference can
be tempting... troublesome... and can make our lives meaningless. Wiesel s purpose is to warn the
people that change is needed in order to encourage the people to make a change in the world for the
better so something as tragic and devastating like the Holocaust never happens again. He establishes a
critical tone for readers by using stylistic devices and rhetorical devices such as imagery,
contemplative questions,and word choice in order to achieve his message that indifference is
inhumane. Wiesel s message about the inhumanity of indifference and the importance of resistance is
still relevant today. Unfortunately, although Elie Wiesel and much of the world hoped nothing like the
Holocaust would ever happen again, there have been other cases of genocide later after the Holocaust.
For example, in the year 1991, the The Bosnian War and Srebrenica Genocide took place ( The
Bosnian War ). In 1991, Yugoslavia began to break up along ethnic lines, causing a surge of ethnic
groups trying to seize the power in government ( The Bosnian War ). When the republic of Bosnia and
Herzegovina declared their independence in 1992 the region quickly became the center of the fighting.
In the article The Bosnian War and Srebrenica Genocide the Serbs were a group who rapidly rose to
power by taking up 90 percent of the military, and by also oppressing any other ethnic group that to
them posed a threat to their rights In addition the article The Bosnian War and Srebrenica Genocide it
states that the height of the killing took place in July 1995. When 8,000 Bosniaks were killed in what
became known as the Srebrenica genocide, the largest massacre in Europe. Despite, all the mass
killings, most of the world
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32. Exchange Rate Between Countries Determines One Currency s...
Lewis Hodge
International Finance
Euro/USD Exchange rate
December 7, 2015
Exchange Rate
The exchange rate between countries determines one currency s strength in comparison to another.
These rates also oversee the economic growth and the currency purchasing power of countries.
Therefore, exchange rates are vital in ensuring that one country can maintain its value versus the US
dollar which is the most dominant currency. Currently, the Euro/USD exchange rate is at 1.0666. This
paper strives to give clear predictions on the value of the Euro against the USD short term and long
term. The rate is expected to continue decreasing or rather the US dollar is expected to gain strength
against the Euro in the near future as a result of the ... Show more content on Helpwriting.net ...
It is unlikely for the Euro to gain stability based on the current market movement and expected market
movement in the next three months. With fed intervention, it is expected to see the Euro/USD
exchange rate rise to 1.05, (Eurorateforecast.com, 2015). The Euro is expected to continue declining
for the next three months and even in the foreseeable future. The declining rate has enabled currencies
such as USD and Sterling Pounds to make gains against the Euro in a very substantial manner. The
declining rate of the Euro/USD exchange rate is expected to follow the trend outlined below in the
next three months. The Euro/USD exchange rate is expected to fall to 1.07 in a period of one month
and then to 1.05 in three months. The latter will see the USD gain strength against the Euro, (Bank,
2015). The strength of Euro mainly depends on the desire of private investors to hold assets in Euros.
The European Central Bank has failed to check the anti inflationary measures that will assist in seeing
the Euro strengthen against the US Dollar. The Central bank has also failed to maintain the price
stability that has facilitated the continued deterioration of the Euro.
The Euro/USD exchange rate is expected to decline further to 0.98 in two years due to the various
factors that
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33. Not Always A Bad Thing Essay
Strangely enough when Jean died he didn t feel any pain. He didn t feel scared, or try to fight it. He
felt calm, ready. He felt it was just his time to go. His friends were screaming, and frantically trying to
save him.
A Titan had grabbed him after his Three Dimensional Movement Gear had malfunctioned and the belt
snapped. He had managed to grab a replacement set and fight off several Titans, but he was just
outmatched.
Jean remembered the Titan picking him up in one massive hand and crushing him. It broke his back
and punctured both lungs before cutting him in half with disgusting and jagged teeth.
After that, nothing.
hr
Jean could see. Not only could he see but he could feel, hear and smell.
Above him was a bright blue sky ... Show more content on Helpwriting.net ...
Th Thomas? He asked in disbelief.
The young man staring at Jean was Thomas Annigan, one of the people who had been training with
hopes of someday joining the Survey Corps. He was killed when the Titans breached wall Rose. Jean
reached out a shaky hand and placed it on Thomas shoulder. He was shocked to find that it was sturdy,
and felt real.
But... You re... Dead.
Thomas frowned and pulled Jean for a hug. Jean froze at the sudden awkward contact. When Thomas
was done hugging him, Jean gestured around the room.
Where are we? What is this place?
Thomas smiled.
It s my house. I ve always wanted one just like this. Surrounded by trees, and there s even a river
nearby.
Jean slowly sat on a nearby chair.
34. But Thomas... You re dead...
Thomas sat in a chair adjacent to Jean and smiled reassuringly.
What s the last thing you remember?
Jean frowned and looked down.
I remember... Titans... They had broken wall Rose again... And I...
Jean clapped a hand over his mouth to stifle a sudden scream.
Oh God... No..!
Thomas patted his shoulder reassuringly.
It s okay Jean. You re in a better place now.
Jean began to quake in anger and sadness.
Better place..? We re dead... How can you be okay with that?
At first I was scared... And so alone. But then some of the others found me and explained what
happened and I felt better. We re all together now, and we re happy.
Jean looked up.
Others? There s more people here?
Thomas nodded.
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35. Hellenistic Weather Patterns
Currently, climate change and erratic weather patterns are among the most prevalent news items.
Moreover, modern day humans have developed a habit of checking the daily weather update, and they
use this information for planning their day activities. Citing the recent weather disasters, such as the
Tsunami, and Hurricane Katrina, weather patterns have become an integral part of the modern day
living. According to a study by Horanont, Phithakkitnukoon and Leong, particular weather conditions
influence human activities when contrasted with people s normal activity patterns. Further, these
activities were noticeable at different time intervals of the day (e81153). Consequently, the weather
has an effect on how people feel and act.
According to Horanont, Phithakkitnukoon and Leong, cold, rainy, and windy weather conditions
influenced human activities significantly (e81153). During the cold and rainy weather days, people s
day and night activities were more diverse. The intensity of the weather condition influenced people s
activities with heavy rainfalls and strong wind speeds days indicating more diversity in terms of
human day and night activities (Horanont, ... Show more content on Helpwriting.net ...
These events have shaped human activities around the weather. Sherman Morris reviewed 25 years
research about human response and activities during severe weather events and found out that various
factors influence human response to weather conditions. According to the research, more than 70
percent of the people in tornado prone areas were aware of the weather event and sought shelter
(Sherman Morris 670). Moreover, people response to a weather condition is based on the perceive
injury, health concern, the likelihood of the event, and the perceived risk level (Sherman Morris 676).
In conclusion, the two studies show that the weather has an effect on how people feel and
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