Papain is a powerful digestive enzyme commonly found in and extracted from papaya. It plays a key role in breaking down toxins and is a digestive aid and antioxidant. It breaks down larger proteins into smaller proteins, peptides, or amino acids by splitting bonds in protein chains. Bromelain is an enzyme found in pineapples, kiwis, mangos, and bananas that conducts similar functions to papain. It is prepared from pineapple plant stumps after fruit harvest by extracting the juice containing the soluble bromelain enzyme. Along with papain, bromelain is commonly used to tenderize meat. Ficin is a protease enzyme derived from fig latex. It is part of the cyst

1. Sapna Srivastava
M.Sc IInd semester
MBT-203 ENZYME TECHNOLOGY

2.  Enzymes are biological molecule that act as
catalyst and help complex reaction occur
everywhere in life.

3.  Lets say you ate a piece of meat. Protease
would go to work and help break down the
peptide bonds between the amino acids

5.  A powerful digestive enzyme commonly
found and extracted from papaya.
 Used as a meat tenderizer
 Plays a key role in the break down of toxins
and is a powerful digestive aid and
antioxidant.
 Breakdown of larger protein into smaller
proteins or peptides or even into the
smallest amino acid subunit by splitting the
bond in the interior of the protein chain or
the end of the chain in a broad Ph range

6.  An enzyme that is the part of the papain
family and conducts similar function.
 It is found in fruits kiwis,pineapple, mangos
and bananas.

7.  Bromelain is prepared from the stump or root
portion of the pineapple plant after harvest
of the fruit. This stump or root portion is
collected from the fields, peeled and
crushed to extract the juice containing the
soluble Bromelain enzyme.
 Roughly 95 plus percent of the meat
tenderizing enzymes consumed in the United
States are from the plant proteases – Papain
and Bromelain.

8.  Along with papain, bromelain is one of the
most popular proteases to use for meat
tenderizing.
 Preparation of meat balls.
 Systemic enzyme therapy (combinations of
proteolytic such as bromelain, trypsin and
papain) has been used in the treatment of
breast cancer.

9.  It is a vegetable based enzyme and it is
derived from figs latex
 It is of a family of proteases known as
cysteine endopeptidases.

10.  Fermented fish sauce is a traditional
 The production procedure consist of mixing
small pieces of fish with high concentration
of salt and storing the mixture at ambient
temperature for 6-18 months
 During the process hydrolytic enzyme slowly
degrade the fish tissue
 Massive proteolysis occurs with the formation
of peptides and amino acid
 The use of enzyme as specific tools for fish
processing operation

11.  Increasing the yield.
 Enhance flavour and improve protein yield.
 Improving the extraction of proteins and
converting by products into valuable
ingredients.
 the consumer sprinkles the powder
containing the standardized enzyme material
on the meat and through a mechanical
process called “forking” have the enzyme
penetrate the meat cut and then
immediately cook in order to produce a
tenderized and highly palatable product.

12.  The action of the enzyme will depend on the
time and temperature that the enzyme has
to work. As an example, a cut of meat may
be injected with a marinade and then
vacuum tumbled to finish absorption and
forming. The meat is then flash frozen and
thawed when ready for use.