Enzymes are proteins that act as catalysts for biochemical reactions by binding to specific substrate molecules. Enzymes have an active site that allows only particular substrates to bind via a "lock and key" mechanism. This binding forms an enzyme-substrate complex and lowers the activation energy needed for the reaction, accelerating the production of products. For example, the activation energy of acid hydrolysis of sucrose is 6.22 kj/mol, but only 2.15 kj/mol in the presence of the sucrose enzyme. The enzyme then changes shape after producing products and can no longer bind substrates.

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2. Mahee ANAM
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3. Mechanism of enzyme action

4. Enzyme & Substrates :
 Enzyme: A substance produced by a living organism that acts as a catalyst to bring
about a specific biochemical reaction.
 Substrates: In biochemistry, the substrate is a molecule upon which an enzyme acts.
Enzymes catalyze chemical reactions involving the substrate(s).
 Enzyme are highly specific in their active site.
 Enzymes and substrates are bind together in a model called “lock and key”.

5. Mechanism of enzyme action:
The substrate molecule bind it self to the active sites on the surface of enzyme. The
active site in a given enzyme is so shaped that only a specific substrate can fit in it. Just
like a lock and key. The specific binding results in the formation of enzyme substrate
complex.
Enzyme lower down the activation energy of reaction.

6. Example:
Activation energy of acid hydrolysis of sucrose is 6.22 kj/mol. But activation
energy in only 2.15 kj/mol. When hydrolysis take place in presence of sucrose
enzyme.

7. Cont.…
 At finally product are produce.
 The active site of the enzyme change shape and can no longer bind to
the substrate. It been denatured.