ELISA .pptx
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ELISA (enzyme-linked immunosorbent assay) is a common laboratory technique used to measure the concentration of an analyte like antibodies or antigens in solution. It works based on the principle of antigen-antibody binding, using an enzyme conjugate attached to a secondary antibody that binds to the antibody-antigen complex. There are different types of ELISA including indirect, direct, sandwich, and competitive. ELISA has many applications like screening blood donations, measuring hormone levels, detecting infections and drugs.
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ELISA- Principle, procedure , types and applications
Enzyme-linked immunosorbent assay (ELISA) is a labeled immunoassay that is considered the gold standard of immunoassays.
This immunological test is very sensitive and is used to detect and quantify substances, including antibodies, antigens, proteins, glycoproteins, and hormones.
The detection of these products is accomplished by complexing antibodies and antigens to produce a measurable result.
Elisa ppt
The enzyme-linked immunosorbent assay (ELISA) is a common laboratory technique used to measure the concentration of an analyte like antibodies or antigens in solution. It works by using the principle of antigen-antibody binding and an enzyme-linked secondary antibody or antigen to detect the presence of an analyte. There are several types of ELISA including direct, indirect, sandwich, and competitive based on the procedure and detection method used. ELISA is a sensitive and specific technique with advantages like using inexpensive and stable reagents, but it also has limitations like possible false positives and negatives.
Elisa and Its types
ENZYME LINKED IMMUNO-SORBENT ASSAY is a method used in different laboratories for the detection of multiple antigens and antibodies.
ELISA: Enzyme-linked Immunosorbent Assay
ELISA or Enzyme-linked Immunosorbent Assay is a qualitative and quantitative assay for detecting the presence of antigens (virus, hormones, enzymes, etc.) in a sample.
Elisa
The document describes the ELISA (Enzyme-Linked Immunosorbent Assay) technique. ELISA involves using an enzyme to detect the binding of an antigen to its antibody. The enzyme converts a colorless substrate to a colored product, indicating the presence of the antigen-antibody binding. There are different types of ELISA including direct, indirect, sandwich, and competitive ELISA. ELISA is used to detect various diseases and was one of the first screening tests for HIV.
ELISA.pptx
ELISA (enzyme-linked immunosorbent assay) is a biochemical technique used to detect the presence of antigens, antibodies, or hormones in a biological sample. It works by detecting the binding of an antibody to its specific antigen using an enzyme to produce a colored product, which can then be measured. There are three main types of ELISA - indirect, sandwich, and competitive - which differ in their detection of antigens or antibodies. ELISA provides quantitative results and is more sensitive than other antibody detection methods, making it useful for applications like disease diagnosis, food testing, and pharmaceutical research.
Enzyme linked immunosorbent assay
ELISA is a biochemical assay technique used in immunology to detect antibodies, antigens, or other substances. It works by immobilizing an antigen or antibody on a plate and detecting its presence with an enzyme-linked antibody or antigen. There are four common ELISA tests based on the binding structure between the antibody and antigen: direct ELISA, indirect ELISA, sandwich ELISA, and competition ELISA. ELISA provides a sensitive, specific, and quantitative method to detect antibodies or antigens in a sample.
Immunoassays powerpoint presentaion
Immunoassays are biochemical methods that use the specificity of antigen-antibody reactions to detect and quantify target molecules in biological samples. The document defines immunoassay and provides details on various types including enzyme immunoassays (ELISA, EMIT), radioimmunoassay (RIA), counting immunoassay (CIA), fluoroimmunoassay (FIA), and chemiluminescence immunoassay (CLIA). It describes the principles, components, procedures, and applications of each type of immunoassay for detecting molecules like antibodies, hormones, and proteins.
Recommended
ELISA- Principle, procedure , types and applications
Enzyme-linked immunosorbent assay (ELISA) is a labeled immunoassay that is considered the gold standard of immunoassays.
This immunological test is very sensitive and is used to detect and quantify substances, including antibodies, antigens, proteins, glycoproteins, and hormones.
The detection of these products is accomplished by complexing antibodies and antigens to produce a measurable result.
Elisa ppt
The enzyme-linked immunosorbent assay (ELISA) is a common laboratory technique used to measure the concentration of an analyte like antibodies or antigens in solution. It works by using the principle of antigen-antibody binding and an enzyme-linked secondary antibody or antigen to detect the presence of an analyte. There are several types of ELISA including direct, indirect, sandwich, and competitive based on the procedure and detection method used. ELISA is a sensitive and specific technique with advantages like using inexpensive and stable reagents, but it also has limitations like possible false positives and negatives.
Elisa and Its types
ENZYME LINKED IMMUNO-SORBENT ASSAY is a method used in different laboratories for the detection of multiple antigens and antibodies.
ELISA: Enzyme-linked Immunosorbent Assay
ELISA or Enzyme-linked Immunosorbent Assay is a qualitative and quantitative assay for detecting the presence of antigens (virus, hormones, enzymes, etc.) in a sample.
Elisa
The document describes the ELISA (Enzyme-Linked Immunosorbent Assay) technique. ELISA involves using an enzyme to detect the binding of an antigen to its antibody. The enzyme converts a colorless substrate to a colored product, indicating the presence of the antigen-antibody binding. There are different types of ELISA including direct, indirect, sandwich, and competitive ELISA. ELISA is used to detect various diseases and was one of the first screening tests for HIV.
ELISA.pptx
ELISA (enzyme-linked immunosorbent assay) is a biochemical technique used to detect the presence of antigens, antibodies, or hormones in a biological sample. It works by detecting the binding of an antibody to its specific antigen using an enzyme to produce a colored product, which can then be measured. There are three main types of ELISA - indirect, sandwich, and competitive - which differ in their detection of antigens or antibodies. ELISA provides quantitative results and is more sensitive than other antibody detection methods, making it useful for applications like disease diagnosis, food testing, and pharmaceutical research.
Enzyme linked immunosorbent assay
ELISA is a biochemical assay technique used in immunology to detect antibodies, antigens, or other substances. It works by immobilizing an antigen or antibody on a plate and detecting its presence with an enzyme-linked antibody or antigen. There are four common ELISA tests based on the binding structure between the antibody and antigen: direct ELISA, indirect ELISA, sandwich ELISA, and competition ELISA. ELISA provides a sensitive, specific, and quantitative method to detect antibodies or antigens in a sample.
Immunoassays powerpoint presentaion
Immunoassays are biochemical methods that use the specificity of antigen-antibody reactions to detect and quantify target molecules in biological samples. The document defines immunoassay and provides details on various types including enzyme immunoassays (ELISA, EMIT), radioimmunoassay (RIA), counting immunoassay (CIA), fluoroimmunoassay (FIA), and chemiluminescence immunoassay (CLIA). It describes the principles, components, procedures, and applications of each type of immunoassay for detecting molecules like antibodies, hormones, and proteins.
Elisa and its type
these slides will let you know about the ELISA and its types and how it is preformed in the laboratory
Enzyme-Linked Immunosorbent Assay.pptx
What is enzyme-linked immunosorbent assay?
A laboratory technique that uses antibodies linked to enzymes to detect and measure the amount of a substance in a solution, such as serum. The test is done using a solid surface to which the antibodies and other molecules stick.
Enzyme Linked Immunosorbent Assay (ELISA)
The document summarizes the enzyme-linked immunosorbent assay (ELISA) technique. It describes the basic process which involves coating a solid surface like a microwell plate with an antigen or antibody, adding an enzyme-linked antibody or antigen to detect binding, washing away unbound reagents, and measuring the enzyme activity using a chromogenic substrate which produces a detectable color change. It discusses different types of ELISA based on detection methods or the antigen-antibody binding procedure. It also lists materials commonly used and provides some advantages and limitations of the ELISA technique.
BIOTECHNOLOGY 2 Lecture 9 ELISA TEST.pptx
ELISA (enzyme-linked immunosorbent assay) is a biochemical technique used to detect the presence of antibodies or antigens in a sample. It involves affixing an unknown antigen to a surface, applying a specific antibody linked to an enzyme, and adding a substrate that the enzyme converts to a detectable signal, most commonly a color change. ELISA has been used as a diagnostic tool in medicine, plant pathology, and the food industry for quality control.
Principles and Applications of ELISA
ELISA (enzyme-linked immunosorbent assay) is a biochemical technique used to detect the presence of antibodies and antigens in a liquid sample. It relies on an enzyme-linked antibody or antigen to detect the target protein. There are different types of ELISA including direct, indirect, sandwich, and competitive. The ELISA process involves coating a plate with an antigen or antibody, adding a sample and enzyme-linked antibody, washing unbound material, and detecting the enzyme's product to quantify the target. ELISAs are widely used in medical testing, food safety, and disease detection.
Elisa
The document provides an overview of enzyme-linked immunosorbent assay (ELISA) and how it is used as a diagnostic tool. It describes the basic immune response process, including how antigens are presented and recognized by B and T cells, leading to antibody production. It then explains the principles of ELISA, noting it detects antibodies or antigens based on antibody-antigen binding. The main types of ELISA - indirect, direct, sandwich and competitive - are defined. Applications like detecting disease infections and allergens are highlighted.
Presentation elisa ppt
ELISA is an immunological technique used to detect the presence of antigens or antibodies in samples. It works by immobilizing an antigen or antibody on a plate and detecting it using an enzyme-linked antibody or antigen. This allows quantification of the analyte being tested for. ELISA has advantages like being sensitive, specific, easy to perform, and having a long shelf life for reagents. It is commonly used to detect proteins, hormones, drugs, tumor markers, antibodies, and antigens in clinical and research applications.
Enzyme-Linked Immunosorbent Assay (ELISA).pptx
The document provides an overview of enzyme-linked immunosorbent assay (ELISA), including its history, principles, requirements, types (direct, indirect, sandwich, competitive), applications (ELISPOT), equipment (washer, reader), and generations used for HIV detection. ELISA is a sensitive technique for detecting antigen-antibody interactions using an enzyme-substrate system for detection. It was developed in 1971 and involves multiple steps with different reagents to separate bound and unbound fractions.
Presentation1 elisa
The ELISA (enzyme-linked immunosorbent assay) is a test that uses antibodies and color change to detect the presence of a substance like an antigen. There are four main types of ELISA - direct, indirect, sandwich, and competitive. The sandwich ELISA involves capturing the antigen between two antibodies, one coated on a surface and one linked to an enzyme. This amplified signal allows the ELISA to be a sensitive diagnostic tool for detecting substances related to diseases, food allergens, and other analytes.
Enzyme linked immunosorbent assay (elisa) and its clinical significance
This document discusses the principle and applications of enzyme-linked immunosorbent assay (ELISA). ELISA uses an enzyme-linked antibody or antigen to detect a protein. It has advantages over radioimmunoassay like being non-radioactive, more stable reagents, and ability to automate. ELISA is used to detect hormones, tumor markers, antigens, and antibodies involved in infectious diseases. Variations include indirect ELISA for antibody detection, sandwich ELISA for antigen detection, and competitive ELISA. The document also discusses other immunoassay techniques like immunofluorescence, immunohistochemistry, EMIT, and lab-on-chip technologies.
Elisa
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones.
Elisa ppt nitub-02
The document provides guidelines for performing ELISA (Enzyme-Linked Immunosorbent Assay), including:
1. ELISA is a popular immunological technique that uses antibodies and enzymes to detect antigens or antibodies. It is very sensitive and can be used both qualitatively and quantitatively.
2. The basic steps of ELISA involve coating a plate with an antigen, adding a primary antibody, adding a secondary antibody linked to an enzyme, adding a substrate that reacts with the enzyme to produce a colored product.
3. Factors that can cause troubleshooting issues include contamination, improper washing, expired reagents, incorrect procedures, temperature issues, poor pipetting technique, and more. Proper controls and validation
Elisa- ENZYME LINKED IMMUNO SORBENT ASSAY
ELISA (enzyme-linked immunosorbent assay) is a sensitive technique used to detect antibodies, antigens, and other proteins. It uses antibodies and color changing enzymes to detect the presence of a substance. There are different types of ELISA including sandwich, indirect, and competitive assays. ELISA uses microplates coated with capture antibodies and enzyme-linked detection antibodies to amplify signals. It has various applications in diagnostics, toxicology, industry, and forensics due to its sensitivity and ability to detect substances in small quantities.
Test for detection of plant virus by ELISA test.pdf
This presentation provides an overview of the Enzyme-Linked Immunosorbent Assay (ELISA) test for the detection of plant viruses. The ELISA test is a highly sensitive and specific method for detecting viral antigens in plant tissues, seeds, and other plant materials.
The presentation covers the principles of ELISA test, including the different types of ELISA tests, such as direct, indirect, sandwich, and competitive ELISA. The steps involved in the ELISA test, including sample preparation, antibody labeling, incubation, and detection, are discussed in detail.
The advantages and limitations of the ELISA test for plant virus detection are also highlighted. The presentation includes a comparison of the ELISA test with other diagnostic methods for plant viruses, such as polymerase chain reaction (PCR) and serological assays.
Finally, the presentation provides examples of the applications of the ELISA test for plant virus detection, including its use in crop protection, disease management, and quarantine measures. The presentation concludes with a summary of the key points discussed and recommendations for the use of the ELISA test in plant virus detection.
elisa ppt class-converted.pptx
ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood. An antibody is a protein produced by the body's immune system when it detects harmful substances, called antigens
elisaLecture_based on new way to medical lab.ppt
The document describes the enzyme-linked immunosorbent assay (ELISA) technique. ELISA uses antibodies and antigen-enzyme conjugates to detect the presence and quantify antigens or antibodies in a sample. It is a sensitive, specific immunoassay that is usually performed in microwell plates, where antibodies or antigens bind to the wells and are detected using enzyme-linked antibodies and color-changing substrates. There are different types of ELISA including sandwich, competitive, and indirect formats.
ELISA
The document discusses the enzyme-linked immunosorbent assay (ELISA), a biochemical technique used to detect antibodies, antigens, or other molecules in a liquid sample. ELISA uses antibodies and color changing enzymes to identify target molecules. It is a sensitive, quantitative, and widely used technique in medicine and research. There are different types of ELISA including competitive, sandwich, and indirect methods depending on the target molecule being detected.
ELISA and its application in clinical researches
The document describes the enzyme-linked immunosorbent assay (ELISA), a commonly used laboratory technique for detecting and quantifying antigens and antibodies. It involves immobilizing an antigen or antibody to a plate and detecting it using an antibody or antigen linked to an enzyme. There are four basic ELISA formats - direct, indirect, sandwich, and competitive - that allow flexibility based on the antibodies and results required. The general ELISA procedure involves coating a plate with a capture antibody or antigen, adding the sample, washing unbound material, adding a detection antibody linked to an enzyme, washing again, and detecting the enzyme's activity using a substrate to produce a measurable signal correlated to analyte concentration.
Elisa
This document provides an overview of Enzyme Linked Immunosorbent Assay (ELISA). ELISA is a biochemical technique used to detect the presence of antibodies or antigens in a liquid sample using enzymes to produce a detectable color change. The basic principle involves an antigen or antibody being immobilized on a plate and detected by an antibody or antigen linked to an enzyme. The document describes the basic components and steps of ELISA including antigen, antibody, equipment, reagents, and different types like direct, indirect, sandwich and competitive ELISA. Advantages include high specificity and sensitivity, while limitations include potential cross-reactivity.
Elisa
This document provides an overview of Enzyme Linked Immunosorbent Assay (ELISA). ELISA is a biochemical technique used to detect the presence of antibodies or antigens in a liquid sample. It relies on the principle of using an enzyme to detect the binding of an antigen-antibody complex. The document discusses the basic components and steps of ELISA including antigen, antibody, equipment, reagents, and different types like direct, indirect, sandwich and competitive ELISA. It also covers troubleshooting, advantages, and applications of this important laboratory technique.
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DNA IS GENETIC METERIAL.pptx
This document summarizes three key experiments in the discovery of DNA as the genetic material:
- In 1927, Frederick Griffith discovered the "transforming principle" through experiments showing that living bacteria could be transformed from harmless to virulent.
- In 1944, Avery, Macleod and McCarty treated bacteria with enzymes that broke down proteins, RNA and DNA, finding that only treatment with DNAses inhibited bacterial transformation, proving DNA was the genetic material.
- In 1952, Hershey and Chase used radioactive labeling to show that DNA, not protein, entered bacteria during viral infection, confirming DNA as the hereditary material.
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these slides will let you know about the ELISA and its types and how it is preformed in the laboratory
Enzyme-Linked Immunosorbent Assay.pptx
What is enzyme-linked immunosorbent assay?
A laboratory technique that uses antibodies linked to enzymes to detect and measure the amount of a substance in a solution, such as serum. The test is done using a solid surface to which the antibodies and other molecules stick.
Enzyme Linked Immunosorbent Assay (ELISA)
The document summarizes the enzyme-linked immunosorbent assay (ELISA) technique. It describes the basic process which involves coating a solid surface like a microwell plate with an antigen or antibody, adding an enzyme-linked antibody or antigen to detect binding, washing away unbound reagents, and measuring the enzyme activity using a chromogenic substrate which produces a detectable color change. It discusses different types of ELISA based on detection methods or the antigen-antibody binding procedure. It also lists materials commonly used and provides some advantages and limitations of the ELISA technique.
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ELISA (enzyme-linked immunosorbent assay) is a biochemical technique used to detect the presence of antibodies or antigens in a sample. It involves affixing an unknown antigen to a surface, applying a specific antibody linked to an enzyme, and adding a substrate that the enzyme converts to a detectable signal, most commonly a color change. ELISA has been used as a diagnostic tool in medicine, plant pathology, and the food industry for quality control.
Principles and Applications of ELISA
ELISA (enzyme-linked immunosorbent assay) is a biochemical technique used to detect the presence of antibodies and antigens in a liquid sample. It relies on an enzyme-linked antibody or antigen to detect the target protein. There are different types of ELISA including direct, indirect, sandwich, and competitive. The ELISA process involves coating a plate with an antigen or antibody, adding a sample and enzyme-linked antibody, washing unbound material, and detecting the enzyme's product to quantify the target. ELISAs are widely used in medical testing, food safety, and disease detection.
Elisa
The document provides an overview of enzyme-linked immunosorbent assay (ELISA) and how it is used as a diagnostic tool. It describes the basic immune response process, including how antigens are presented and recognized by B and T cells, leading to antibody production. It then explains the principles of ELISA, noting it detects antibodies or antigens based on antibody-antigen binding. The main types of ELISA - indirect, direct, sandwich and competitive - are defined. Applications like detecting disease infections and allergens are highlighted.
Presentation elisa ppt
ELISA is an immunological technique used to detect the presence of antigens or antibodies in samples. It works by immobilizing an antigen or antibody on a plate and detecting it using an enzyme-linked antibody or antigen. This allows quantification of the analyte being tested for. ELISA has advantages like being sensitive, specific, easy to perform, and having a long shelf life for reagents. It is commonly used to detect proteins, hormones, drugs, tumor markers, antibodies, and antigens in clinical and research applications.
Enzyme-Linked Immunosorbent Assay (ELISA).pptx
The document provides an overview of enzyme-linked immunosorbent assay (ELISA), including its history, principles, requirements, types (direct, indirect, sandwich, competitive), applications (ELISPOT), equipment (washer, reader), and generations used for HIV detection. ELISA is a sensitive technique for detecting antigen-antibody interactions using an enzyme-substrate system for detection. It was developed in 1971 and involves multiple steps with different reagents to separate bound and unbound fractions.
Presentation1 elisa
The ELISA (enzyme-linked immunosorbent assay) is a test that uses antibodies and color change to detect the presence of a substance like an antigen. There are four main types of ELISA - direct, indirect, sandwich, and competitive. The sandwich ELISA involves capturing the antigen between two antibodies, one coated on a surface and one linked to an enzyme. This amplified signal allows the ELISA to be a sensitive diagnostic tool for detecting substances related to diseases, food allergens, and other analytes.
Enzyme linked immunosorbent assay (elisa) and its clinical significance
This document discusses the principle and applications of enzyme-linked immunosorbent assay (ELISA). ELISA uses an enzyme-linked antibody or antigen to detect a protein. It has advantages over radioimmunoassay like being non-radioactive, more stable reagents, and ability to automate. ELISA is used to detect hormones, tumor markers, antigens, and antibodies involved in infectious diseases. Variations include indirect ELISA for antibody detection, sandwich ELISA for antigen detection, and competitive ELISA. The document also discusses other immunoassay techniques like immunofluorescence, immunohistochemistry, EMIT, and lab-on-chip technologies.
Elisa
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones.
Elisa ppt nitub-02
The document provides guidelines for performing ELISA (Enzyme-Linked Immunosorbent Assay), including:
1. ELISA is a popular immunological technique that uses antibodies and enzymes to detect antigens or antibodies. It is very sensitive and can be used both qualitatively and quantitatively.
2. The basic steps of ELISA involve coating a plate with an antigen, adding a primary antibody, adding a secondary antibody linked to an enzyme, adding a substrate that reacts with the enzyme to produce a colored product.
3. Factors that can cause troubleshooting issues include contamination, improper washing, expired reagents, incorrect procedures, temperature issues, poor pipetting technique, and more. Proper controls and validation
Elisa- ENZYME LINKED IMMUNO SORBENT ASSAY
ELISA (enzyme-linked immunosorbent assay) is a sensitive technique used to detect antibodies, antigens, and other proteins. It uses antibodies and color changing enzymes to detect the presence of a substance. There are different types of ELISA including sandwich, indirect, and competitive assays. ELISA uses microplates coated with capture antibodies and enzyme-linked detection antibodies to amplify signals. It has various applications in diagnostics, toxicology, industry, and forensics due to its sensitivity and ability to detect substances in small quantities.
Test for detection of plant virus by ELISA test.pdf
This presentation provides an overview of the Enzyme-Linked Immunosorbent Assay (ELISA) test for the detection of plant viruses. The ELISA test is a highly sensitive and specific method for detecting viral antigens in plant tissues, seeds, and other plant materials.
The presentation covers the principles of ELISA test, including the different types of ELISA tests, such as direct, indirect, sandwich, and competitive ELISA. The steps involved in the ELISA test, including sample preparation, antibody labeling, incubation, and detection, are discussed in detail.
The advantages and limitations of the ELISA test for plant virus detection are also highlighted. The presentation includes a comparison of the ELISA test with other diagnostic methods for plant viruses, such as polymerase chain reaction (PCR) and serological assays.
Finally, the presentation provides examples of the applications of the ELISA test for plant virus detection, including its use in crop protection, disease management, and quarantine measures. The presentation concludes with a summary of the key points discussed and recommendations for the use of the ELISA test in plant virus detection.
elisa ppt class-converted.pptx
ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood. An antibody is a protein produced by the body's immune system when it detects harmful substances, called antigens
elisaLecture_based on new way to medical lab.ppt
The document describes the enzyme-linked immunosorbent assay (ELISA) technique. ELISA uses antibodies and antigen-enzyme conjugates to detect the presence and quantify antigens or antibodies in a sample. It is a sensitive, specific immunoassay that is usually performed in microwell plates, where antibodies or antigens bind to the wells and are detected using enzyme-linked antibodies and color-changing substrates. There are different types of ELISA including sandwich, competitive, and indirect formats.
ELISA
The document discusses the enzyme-linked immunosorbent assay (ELISA), a biochemical technique used to detect antibodies, antigens, or other molecules in a liquid sample. ELISA uses antibodies and color changing enzymes to identify target molecules. It is a sensitive, quantitative, and widely used technique in medicine and research. There are different types of ELISA including competitive, sandwich, and indirect methods depending on the target molecule being detected.
ELISA and its application in clinical researches
The document describes the enzyme-linked immunosorbent assay (ELISA), a commonly used laboratory technique for detecting and quantifying antigens and antibodies. It involves immobilizing an antigen or antibody to a plate and detecting it using an antibody or antigen linked to an enzyme. There are four basic ELISA formats - direct, indirect, sandwich, and competitive - that allow flexibility based on the antibodies and results required. The general ELISA procedure involves coating a plate with a capture antibody or antigen, adding the sample, washing unbound material, adding a detection antibody linked to an enzyme, washing again, and detecting the enzyme's activity using a substrate to produce a measurable signal correlated to analyte concentration.
Elisa
This document provides an overview of Enzyme Linked Immunosorbent Assay (ELISA). ELISA is a biochemical technique used to detect the presence of antibodies or antigens in a liquid sample using enzymes to produce a detectable color change. The basic principle involves an antigen or antibody being immobilized on a plate and detected by an antibody or antigen linked to an enzyme. The document describes the basic components and steps of ELISA including antigen, antibody, equipment, reagents, and different types like direct, indirect, sandwich and competitive ELISA. Advantages include high specificity and sensitivity, while limitations include potential cross-reactivity.
Elisa
This document provides an overview of Enzyme Linked Immunosorbent Assay (ELISA). ELISA is a biochemical technique used to detect the presence of antibodies or antigens in a liquid sample. It relies on the principle of using an enzyme to detect the binding of an antigen-antibody complex. The document discusses the basic components and steps of ELISA including antigen, antibody, equipment, reagents, and different types like direct, indirect, sandwich and competitive ELISA. It also covers troubleshooting, advantages, and applications of this important laboratory technique.
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Enzyme linked immunosorbent assay (elisa) and its clinical significance
Enzyme linked immunosorbent assay (elisa) and its clinical significance
Test for detection of plant virus by ELISA test.pdf
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TOPIC NAME Structure of DNA and their Type.pptx
DNA has a double helix structure that resembles a twisted ladder. It is composed of nucleotides, each containing a sugar, phosphate group, and one of four nitrogen bases (A, T, C, or G). The bases on opposite strands bond together via hydrogen bonds between A-T and C-G to form the structure. Erwin Chargaff discovered that the amounts of A and T are equal, as are the amounts of C and G in any DNA sample. There are three types of DNA structures: A-DNA, B-DNA, and Z-DNA.
LAC OPERON SYSTEM.pptx
The document describes the lac operon system, which was the first operon described in 1961 and controls the catabolism of lactose in prokaryotes. The lac operon has 5 structural units - a promoter, operator, three structural genes (LacZ, LacY, LacA) that encode enzymes for lactose breakdown, CAP binding sites, and a regulatory gene. When lactose is absent, a repressor protein binds to the operator, preventing transcription of the structural genes. When lactose is present, it binds to the repressor and causes a conformational change that releases it from the operator, allowing transcription and the breakdown of lactose.
LAC OPERON 2.pptx
The lac operon is a cluster of three genes in prokaryotes that are involved in processing the sugar lactose. It consists of a promoter, operator, and structural genes. The lac operon is inducible, meaning it is only transcribed when lactose is present. Depending on whether lactose and glucose are present or absent, the lac operon will be either actively transcribed or transcriptionally inactive.
DNA STRUCTURE AND TYPES.pptx
DNA is a double-stranded molecule composed of nucleotides that contain nitrogenous bases, a deoxyribose sugar, and a phosphate group. The bases--cytosine, thymine, adenine, and guanine--form rungs of a twisted ladder structure, while the alternating sugar and phosphate molecules form the sides. Watson and Crick discovered that the bases pair up specifically through hydrogen bonds to form the DNA double helix, which contains the genetic information needed to build an organism. DNA can take on different secondary structures including A-DNA, B-DNA, and Z-DNA forms.
TOPIC-DNA repair mechanism and their types.pptx
DNA repair mechanisms are necessary to correct damage to DNA that could otherwise lead to mutations and cancer. There are several types of DNA repair mechanisms that address different types of damage, including base repair through excision, resynthesis and ligation of bases, nucleotide excision repair to address mismatches, and mismatch repair to fix errors. Double stranded breaks can be repaired through non-homologous end joining or homologous recombination repair.
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- 2. ELISA: (Enzyme-linkedimmunosorbent assay ) Definition: • The enzyme-linked immunosorbent assay (ELISA) is a common laboratory technique which is used to measure the concentration of an analyte (usually antibodies or antigens) in solution. EL I SA
- 3. HISTORY OF ELISA Prof-Eva Engvall (1971) .................. Rosalyn Sussman (1960) ...............
- 4. Basic Terms: Solid Phase: •Usually a microtiter plate well, having 8×12 well format. Adsorption: •The process of adding an antigen/antibody, diluted in buffer, so it attaches to the solid phase on incubation. Washing: •The simple flooding & emptying of wells with a buffered solution to separate bound from un-bound reagents in ELISA. Antigen: •Any molecule that elicits the production of antibodies when introduced into body. Antibodies: •Proteins produced in response to antigenic stimuli. Enzyme conjugate: An enzyme that is attached irreversibly to an antibody. e.g: Horse-redish peroxidase (HRPO).
- 5. Basic Terms: Chromogen: Stopping: Reading: Principle of ELISA: Based on Basic Immunology Response Lock and Key Concept: 1) Antigen (key) 2) Antibody (lock): –Key fits into the lock Enzyme conjugate substrates • Bound to a secondary antibody that binds with the antibody- antigen complex.
- 6. TYPES OF ELISA INDIRECT ELISA DIRECT ELISA SANDWICH ELISA NON -COMPETETIVE ELISA 1) Colorimetric ELISA: 2) Chemiluminescent ELISA: 3) Competitive Fluorescence ELISA (On the Basis of Detection) (on the basis of procedure)
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- 8. PROCEDURE OF ELISA:- (FINAL PLATE OF ELISA)
- 9. COMPETETIVE ELISA • ADVANTAGES: Suitable for complex (crude or impure) samples, since the antigen does not require purification prior to measurement. • DISADVANTAGES: Each antigen may require a different method to couple it to the enzyme.
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- 11. THANK YOU