Gel electrophoresis

1. Gel Electrophoresis
Presented by:
Amany ELshamy

2. • Introduction
• What is the electrophoresis?
• Principle
• Material and steps
• Equipment
• Application
• Advantages
• Disadvantages
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3. • The term electrophoresis was coined from a
Greek word “Phoresis” which means “Being
Carried Away”.
• Electrophoresis= to carry with electricity.
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4. • It is a technique used to separate DNA
fragments according to their size by using an
electrical current.
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5. TYPES OF
ELECTROPHORESIS
Zone
ELECTROPHORESIS
Free-flow
ELECTROPHORESIS
Gel
ELECTROPHORESIS
Cellulose acetate
ELECTROPHORESIS
Paper
ELECTROPHORESIS
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6. Gel
ELECTROPHORESIS
Poly acrylamide
ELECTROPHORESIS
Starch
ELECTROPHORESIS
Agarose gel
ELECTROPHORESIS
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7. • Any charged ion or molecule migrates when
placed in an electric field.
• The rate of migration depend upon its net:
Charge
Size
Shape
The applied electric current.
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• Ethidium Bromide.
• Gel Loading Dye.
• DNA ladder.

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• To separate DNA fragments from each other.
• To determine the sizes of DNA fragments.
• To determine the presence or amount of
DNA.

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• Ease of operation as the Gel casted horizontally.
• Short analysis time.
• Non-toxic and low waste generation.
• Low sample consumption and Separate large
molecules (Commonly used for DNA separations).
• Staining can be done before or pouring the gel.
• It does not denaturant the DNA sample.

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• The gel can melt during electrophoresis and
thus may lead to change the DNA shapes.
• Electrophoresis is excellent at separating and
identifying medium- to large-sized
biomolecules.
• Low throughput.

20. • https://www.youtube.com/watch?v=vq759wK
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• https://www.youtube.com/watch?v=saJIWFU
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• https://www.youtube.com/watch?v=ZDZUAle
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