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TECHNICAL SESSION - II 
MITIGATION OF GREEN HOUSE GASES AND 
PROFITABLE ANIMAL FARMING
Topic of presentation 
IMPACT OF USAGE OF AYURVET 
HERBAL SPECIALITIES TO MEET 
CHALLENGES OF CLIMATIC CHANGE
CONTENTS 
1 INTRODUCTION TO CLIMATE CHANGE AND GW 
GLOBAL GHG MITIGATION STRATEGIES 
www.themegallery.com 
2 
3 
PROFITABLE ANIMAL FARMING WITH HERBAL 
SPECIALITIES & MEETING CHALLENGES OF CLIMATE 
CHANGE
H
(2090-2099 relative to 1980-1999) 
0 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7 7.5 
Continued emissions would lead to further warming of 1.4oC to 5.8oC over the 21st century 
Reference: UNFCCC, 2005 
(oC) 
Implications of climate change :
Mean sea level is expected to rise by 9-88 cm by 2100, causing floods, shift of climatic 
zones & other impacts 
Reference : FAO, Livestock long shadow report, (2006)
Expected Impacts on Agriculture 
by 2020-2080 
 30% by 2050 in Central and South 
Asia 
 30% by 2080 in Latin America 
 50% by 2020 in some African 
countries 
8 
Possible yield reduction in agriculture: 
Slide adapted from Dr. R K Pachauri ‘s presentation, State of the World Symposium, Washington, 15th January 
2009.
 GW & ITS IMPACT COULD WELL DENY PEOPLE TO 
ACCESS THE BASIC NECESSITIES. 
 RISK EMANATING FROM GHGS IS NO LONGER A 
MATTER OF SPECULATION 
CH4 
NO2 
CO2 
OZONE 
WATER 
VAPOR 
S 
CFC
Global anthropogenic 
GHG emissions (2009) 
Sources: IPCC (2007) AR4 WGI & WGIII 
Greenhouse Gas Principal Sources (and 
Sinks) 
GWP* 
Carbon Dioxide (CO2) Fossil fuel use, land use 
change 
(oceans, terrestrial 
biosphere) 
1 
Methane (CH4) Fossil fuel 
mining/distribution, 
livestock, rice agriculture, 
landfills 
21 
Nitrous Oxide (N2O) Agriculture and associated 
land use change 
310 
“F-gases” Hydrofluorocarbons 
(HFCs), Perfluorocarbons (PFCs), 
Sulphur Hexafluoride (SF6) 
Industrial processes 140 - 
23,900 
*GWP = Global Warming Potential from Second Assessment Report, as used for reporting purposes under the UNFCCC 
10
GLOBAL 
WARIMNG 
AND 
CLIMATE 
CHANGE 
CONTRIBUTION OF LIVESTOCK 
CONTRIBUTION OF SWINE 
CONTRIBUTION OF POULTRY
GLOBAL LIVESTOCK DISTRIBUTION
Deforest 
ation & 
desertifi 
cation, 
35% 
Manure, 
30% 
Artificial 
fertiliser 
s 3-4% 
Enteric 
fermenta 
tion, 
25% 
Other 
4% 
Animal agriculture is 
responsible for 18% of 
global greenhouse gas 
emissions: 
Methane (CH4) 
Nitrous Oxide (N2O) 
Carbon dioxide (CO2)
Livestock industry 
Emissions: 
The single largest source of methane & nitrous oxide 
37% of global methane emissions 
- 23x more potent than CO2 over 100 years 
- 68x more potent than CO2 over 20 years 
65% of global nitrous oxide emissions 
- 297x more potent than CO2 
64% of the world’s ammonia 
- contributing to acid rain 
- affecting biodiversity 
9% of total CO2 emissions 
Source: 
- “Livestock’s Long Shadow Rome 2006 ” (PDF). Ch. 3, P. 82, 112, 114. Retrieved on 17-01-2009. 
http://tinyurl.com/96csxh
GLOBAL METHANE EMISSIONS FROM ENTERIC FERMENTATION 
Emissions (million tonnes CH4 per year by source) 
Region/country Dairy cattle Other cattle Buffaloes Sheep and 
goats 
Pigs Total 
Sub-Saharan Africa 2.30 7.47 0.00 1.82 0.02 11.61 
Asia * 0.84 3.83 2.40 0.88 0.07 8.02 
India 1.70 3.94 5.25 0.91 0.01 11.82 
China 0.49 5.12 1.25 1.51 0.48 8.85 
Central and South 
America 
3.36 17.09 0.06 0.58 0.08 21.17 
West Asia & North 
Africa 
0.98 1.16 0.24 1.20 0.00 3.58 
North America 1.02 3.85 0.00 0.06 0.11 5.05 
Western Europe 2.19 2.31 0.01 0.98 0.20 5.70 
Oceania and Japan 0.71 1.80 0.00 0.73 0.02 3.26 
Eastern Europe and CIS 1.99 2.96 0.02 0.59 0.10 5.66 
Other developed 0.11 0.62 0.00 0.18 0.00 0.91 
Total 15.69 50.16 9.23 9.44 1.11 85.63 
Source: FAO (2007)
100 million tonnes of methane each year 
SOURCE: GHG online, Methane, sources, sinks & science
N 
O EMISSIONS FROM LIVESTOCK (million tonnes per year) 
2 
Region/country Dairy cattle Other cattle Buffalo Sheep and 
goats 
Pigs Poultry Total 
Sub-Saharan Africa 0.06 0.21 0.00 0.13 0.01 0.02 0.43 
Asia * 0.02 0.14 0.06 0.05 0.03 0.05 0.36 
India 0.03 0.15 0.06 0.05 0.01 0.01 0.32 
China 0.01 0.14 0.03 0.10 0.19 0.10 0.58 
Central and South 
0.08 0.41 0.00 0.04 0.04 0.05 0.61 
America 
West Asia and North 
Africa 
0.02 0.03 0.00 0.09 0.00 0.03 0.17 
North America 0.03 0.20 0.00 0.00 0.04 0.04 0.30 
Western Europe 0.06 0.14 0.00 0.07 0.07 0.03 0.36 
Oceania and Japan 0.02 0.08 0.00 0.09 0.01 0.01 0.21 
Eastern Europe and 
CIS 
0.08 0.08 0.00 0.03 0.04 0.02 0.28 
Other developed 0.00 0.03 0.00 0.02 0.00 0.00 0.06 
Total 0.41 1.64 0.15 0.68 0.44 0.36 3.69 
Source: FAO (2007)
Relative emission of GHG from different meat types 
COMMODITY C0% CH4 % N2O % 
Pig meat 47 22 53 
Chicken 45 2 58 
Beef 21 49 31 
Sheep meat/ 
Chevon 
19 50 31 
Milk 24 49 27 
Eggs 46 4 50 
Reference: Williams, a.g., Adsley, E., Sandras, D.L. Determining the environmental burdens and resource use in the 
production of agriculture and horticulture commodities. Defra Research report, 2007. 
The FAO report states that current production levels of meat contribute between 14 and 
22 percent of the 36 billion tons of "CO2-equivalent" greenhouse gases the world 
produces every year. - Scientific American, Feb, 2009
Meat consumption is expected to double by 2050 
 Raising animals for food is a major cause of global 
warming and yet the global livestock sector is growing 
faster than any other agricultural sub-sector. 
 Dairy output is expected to more than double by 2050. 
 Animal Protein vs. Plant protein 
Source: 
-UN FAO, Livestock’s Long Shadow, Rome 2006 
-The livestock industry and climate – EU makes bad worse, Jens Holm & Toivo Jokkala, 
Parliamentary Group (p. 17-18) 
livestock industry 
FUTURE OUTLOOK
“Livestock’s contribution to environmental problems is on 
a massive scale and its potential contribution to their 
solution is equally large. The impact is so significant that it 
needs to be addressed with urgency.” 
- U.N. Food & Agriculture Organization, 2006
The fastest way to slow global warming and curb 
climate change? 
Cut down on short-lived greenhouse gases such 
as Methane – the single largest source of 
which is livestock.
 Methane is one of the 6 GHGs identified under United Nations Framework 
Convention on Climatic Change UNFCCC Kyoto Protocol .This GHG has 21 
times more GWP than CO 
2 
 Methane is more than 21 times as effectual as CO2 at entrapping 
heat in the atmosphere. 
 A shorter chemical half life of 12 yrs as compared to 120 yrs of CO 
2 
 Even if the entire world switched to a zero carbon economy and lifestyle 
tomorrow, it would take 1000’s years for CO2 to dissipate out of the 
atmosphere. 
 Cutting down on short-lived GHGs will quickly translate into cooling of the 
Earth which will give us time to deal with CO2 emissions. 
Source: 
IPCC 4th Assessment, IPCC Fourth Assessment Report – Synthesis Report 2007 http://www.ipcc.ch/ipccreports/ar4-syr.htm, 
chapter 2 http://ipcc-wg1.ucar.edu/wg1/wg1-report.html
RUMEN 
80-95% 
www.themegallery.com 
INTESTINE 
5-20 % 
Methane 
produced/day/ 
animal: 
250-500 lit
ORGANIC MATTER 
BACTERIA 
PROTOZOA 
FUNGI ATP H2 
VFA CO2 CH4 
www.themegallery.com 
CO 
2 
+ 4H 
2 
→ CH 
4 
+ 2H 
O 
2
US AGENCIES BROUGHT HIGH LEVEL FOCUS ON METHANE 
Supporting projects in Argentina, 
Brazil, China, Colombia, Ecuador, 
India, Korea, Mexico, Mongolia, 
Nigeria, Poland, Philippines, Russia, 
Thailand, Ukraine and Vietnam. 
Provides technical assistance 
and capacity building to 
ensure long-term project 
success of projects BY 
Successful engagement of key 
developing countries
• Some cattle and sheep naturally 
Flatulence Vaccine 
produce less methane. Efforts are 
underway Another area to of determine study has the been gene the 
or 
mapping genes involved. of the methane Farmers genome, could which 
then 
a breed New Zealand for lower research greenhouse organization has 
gas 
producing accomplished. livestock. With this knowledge As with they 
the 
will flatulence work toward vaccine, producing ensuring a vaccine that 
to 
prevent belching and flatulence. Vaccine 
new development problems and are refinement not created may take 
in 
some the effort time. 
to genetically manage 
existing ones will be key to taking 
advantage of this inherited trait. 
• Source:http://www.suite101.com/content/managing-methane- 
from-livestock-a58094#ixzz1IB2E9vOO 
SOURCE: 
http://www.suite101.com/content/managing-methane- 
from-livestock-a58094#ixzz1IB3XyljY 
A RESERCH IN NEWZEALAND 
The 45 million sheep and 10 million cattle in 
New Zealand burped and farted about 90 
percent of that country's methane emissions, 
according to government figures.
Argentina has more than 55 
million cows, making it a leading 
producer of beef. In the study, 
the scientists were surprised to 
discover that a standard 550-kg 
cow produces between 800 to 
1,000 liters of emissions, 
including methane, each day. 
PhysOrg.com) -- In an attempt to understand the extent of cow flatulence on global 
warming, scientists in Argentina are strapping plastic bags to the backs of cows to 
capture their emissions.
'Burpless' Grass Cuts Methane Gas From Cattle, May Help Reduce Global 
Warming 
• Scientists in a joint biotech 
venture by Australia’s 
Molecular Plant Breeding 
Cooperative Research Centre 
and New Zealand rural 
services group PGG Wrightson 
Genomics, are developing a 
grass that will not only cut the 
amount of methane cows 
burp up when chewing the 
cud but also grow in hotter 
climes. 
Grass that may help tackle global 
warming by cutting the level of 
methane given off by cows is in the 
works. (Credit: USDA) 
Ref: Science Daily, May 8, 2010 
• Scientists in a joint biotech 
venture by Australia’s 
Molecular Plant Breeding 
Cooperative Research Centre 
and New Zealand rural 
services group PGG Wrightson 
Genomics, are developing a 
grass that will not only cut the 
amount of methane cows 
burp up when chewing the 
cud but also grow in hotter 
climes.
Unusual Feed Supplement Could Ease Gassy Cows, Reduce Their Greenhouse Gas 
Emissions 
• In a series of laboratory 
experiments conducted by 
Penn State Dairy Scientists, 
and in a live animal test, an 
oregano-based supplement 
not only decreased methane 
emissions in dairy cows by 40 
percent, but also improved 
milk production, according to 
Alexander Hristov, an 
associate professor of dairy 
nutrition. 
Cow belches, a major source of greenhouse 
gases, could be decreased by an unusual feed 
supplement developed by a Penn State dairy 
scientist. (Credit: iStockphoto/Fabian Rothe) 
Ref: Science Daily, Sept 8, 2010
Fish Oils Reduce Greenhouse Gas Emissions From Flatulent Cows 
• The benefits to animals of omega 3 
fatty acids in fish oils have been 
well documented – helping the 
heart and circulatory system, 
improving meat quality and 
reducing methane emissions. 
Researchers from University College 
Dublin reported that by including 
2% fish oil in the diet of cattle, they 
achieved a reduction in the amount 
of methane released by the 
animals. New research shows that fish oils can 
improve meat quality and reducing 
methane emissions in cows. (Credit: 
iStockphoto) 
ScienceDaily (Mar. 30, 2009)
45 
40 
35 
30 
25 
20 
15 
10 
5 
0 
BIOLOGICAL CONTROL 
VACCINATION 
HERD MANAGEMENT 
Low Medium High 
Mitigation % 
DIETARY 
SUPPLEMENTS 
BREEDING 
BMPS 
RUMEN MANIPULATION 
Immediate Longer Term 
Eckard, Grainger & de Klein 2010 
Likely Impact
1. Studies on methane mitigation in goats fed roughage based diets by 
supplementing polyherbal products, thesis submitted to Durg veterinary college, 
Durg, Chattisgarh, 2012. 
2. Effects of herbs, spices and essential oils and their combination on rumen 
microbial fermentation and methane production in vitro. Chaouki Benchaar, Ph.D. 
Agriculture and Agri-Food Canada Dairy and Swine Research and Development 
Centre Sherbrooke, Quebec, Canada JIM 0C8, 2013. 
3. Efficacy of herbal formulation on digestibility, nutrient utilization & methane 
mitigation in sheep” submitted by Dr. Prabhat Kr. Pankaj, ARS, Senior Scientist, 
Central Research Institute for Dryland Agriculture (CRIDA), Indian Council of 
Agricultural Research, Santosh Nagar, Hyderabad, A.P. -500 059 (India), 2014.
Thesis Submitted by: Dr. Subhanker Nanda 
GUIDE: DR. S.P.TIWARI 
PROFESSOR & HEAD, 
DEPT. OF ANIMAL NUTRITION 
COLLEGE OF VETERINARY & ANIMAL 
SCIENMCES, 
ANJORA, DURG, CHATTISGARH, PIN: 491001 
www.themegallery.com 
Thesis
PARTICULARS TREATMENTS 
T0 T1 T2 T3 
No. of Animal 6 6 6 6 
Basal Diet 
Concentrate 
Roughage 
250 g 
ad 
libitum 
250g 
ad libitum 
250g 
ad libitum 
250g 
ad libitum 
Polyherbal 
Product 
(0-21 days; 
30-51 days 
60-81 days) 
- Ruchamax 
(@10gm/day) 
AV/DAC/16 
(@10gm/day) 
Optirum 
(@10gm/day) 
Reference : Studies on methane mitigation in goats fed roughage based diet & its mitigation by 
supplementing polyherbal products . Thesis submitted to Durg Veterinary College, Chattisgarh, 
India, 2012. 
www.themegallery.com
RUMINAL 
ENZYMES 
PROTOZOAL 
COUNT & 
RUMINALMOTILITY 
GROWTH PERFORMANCE, 
DIGESTIBILITY & METABOLIC 
RUMEN FERMENTATION 
PATTERN & BIOCHEMICAL 
PARAMTERES 
www.themegallery.com 
STUDIES 
DRY MATTER 
INTAKE & 
METHANE 
PRODUCTION 
FEED ANALYSIS
in vitro studies 
Particulars 
Treatments 
T0 T1 T2 T3 
Syringe (no.) 4 4 4 4 
Feed substrate 
(basal diet) 0.2g 0.2g 0.2g 0.2g 
Anaerobic medium 
(SRL) 
30ml 30ml 30ml 30ml 
Polyherbal product 
- Ruchamax 
@0.002g/day 
Ruchamax 
@0.002g/day 
Optirum 
@0.002g/day 
The in vitro gas production test was performed as per Menke and Steingass (1988). 
Estimation of methane was carried out in Nucon-5765 gas chromatograph 
equipped with a stainless still column packed with Porapak-Q. 
Studies on methane mitigation in goats fed roughage based diets by supplementing polyherbal products, thesis submitted to Durg 
veterinary college, Durg, Chattisgarh, 2012.
In vitro Trial photos
IN VITRO METHANE GAS PRODUCTION (ml/g 
DM) 
50 55 60 65 70 
Optirum 
Ruchamax N 
Ruchamax 
Control 
69.29 
61.76 
67.51 
59.64 
Methane (ml/g DM) 
OPTIRUM: 13.92% reduction in Methane production 
RUCHAMAX : 10.86 % reduction in Methane production 
RUCHAMAX N: 2.56 % reduction in Methane production
OMD (%) 
30.9 32.36 30.37 
36.67 
40 
30 
20 
10 
0 
Control Ruchamax Ruchamax N Optirum 
In vitro organic matter digestibility is higher in treated group than 
control, higher is the digestibility lower is the production of methane 
•in vitro gas production test was performed as per Menke and Steingass (1988).
4.1 
5.8 5.05 6.05 
8 
6 
4 
2 
0 
Control Ruchamax Ruchamax 
N 
Optirum 
T 
V 
F 
A 
DAY 21 
4.23 5.72 5.13 6.12 
8 
6 
4 
2 
0 
Control Ruchamax Ruchamax 
N 
Optirum 
DAY 45 
4.15 
DAY 90 Increase in TVFA concentration in 
5.85 5.39 6.08 
8 
6 
4 
2 
0 
Control Ruchamax Ruchamax 
N 
Optirum 
treated groups might be due to 
significant increase in fermentation 
of carbohydrates and 
simultaneously more shifting of 
methane energy toward the 
production of propionic acid. 
46.5% 
INCREASE
66 
64 
62 
60 
58 
61.45 61.69 
63.73 
65.05 
DAY 21st 
65 62.9 
64 
63 
62 
61 
DAY 45th 
64.87 
63.21 63.43 
70 
65 
60 
55 
67.9 65.04 
62.37 
60.58 
90 
POOR IS THE FIBRE DIGESTION 
HIGHER IS THE 
CONCENTRATION OF ACETATE 
& MORE IS THE METHANE 
PRODUCTION 
Day45th: ns 
Day90th : s 
10.78% 
DECREASE
PERIOD 
(days) 
GROUPS 
CONTROL RUCHAMAX RUCHAMAX 
N 
OPTIRUM SIGNIFICANCE 
21 15.67±0.49 16.35±0.18 16.63±0.07 16.38±0.08 NS 
45 16.17±0.08 a 17.03±0.20 bc 16.53±0.17 ab 17.50±0.3 d ** 
90 16.53±0.18 a 17.50±0.22 a 17.52±0.39 a 19.31±0.4 b ** 
Average 16.12±0.19 a 16.96±0.18 b 16.89±0.17 b 17.73±0.34 c ** 
Better is the nutrient digestion, lower is the methane production and 
greater is the shifting the H+ ions for the production of useful energy in the 
body in the form of propionic acid.
3.18 
Day 90th 
2.43 2.11 2.47 
3.5 
3 
2.5 
2 
1.5 
1 
0.5 
0 
Control Ruchamax Ruchamax N Optirum 
There was a significant reduction of protozoal population by 22.3% in T3 and 
23.65% in T2 (Ruchamax). The comparative suppression of the protozoal population 
might have reduced the methane production in these groups.
HOLOTRICHS (% IN RL) 
69 
68 
67 
66 
65 
64 
63 
62 
61 
60 
59 
Control Ruchamax Ruchamax 
N 
Optirum 
68.61 
62.94 
66.67 
62.28 
holotrichs 
Holotrichs are higher in the 
fibrous and sugar based diet. 
In the present study Holotrichs 
were higher in control due to 
poor fibre digestibility 
The reduction in protozoal 
numbers in treatments groups 
were due to presence of 
defaunating principles of the 
herbs. 
9.22% 
DECREASE
• PROTOZOA 
• BACTERIA 
DEFAUNATION 
BIOCHEMICAL 
MODULATION • Xylanse 
• TVFA, APB 
• TOTAL-N 
• NH3-N2 
• Protease 
ENZYMATIC 
MODULATION
PARAMETERS NUTRIENT BALANCE (%) 
CONTROL RUCHAMAX RUCHAMAX 
N 
OPTIRUM 
NITROGEN BALANCE 62.79±1.95 64.38±1.86 63.37±1.05 67.54±1.88 
CALCIUM BALANCE 68.5±4.65 69.8±4.85 69.12±4.75 70.1±4.58 
PHOSPHORUS 
BALANCE 
72.4±2.58 76.5±2.15 73.5±1.65 78.5±2.01 
 the percent N balance (%) & Phosphorus balance was significantly higher in group 
T3 and T1 over control 
 the percent Ca balance (%) & Phosphorus balance was significantly higher in group 
T3 over control, T1 
& T2were non-significantly different
PERIOD 
(days) 
GROUPS 
CONTROL RUCHAMAX RUCHAMAX 
N 
OPTIRUM SIGNIFICANCE 
21 79.75±3.31 84.03±1.62 83.03±2.18 86.23±2.55 NS 
45 82.28±1.75 86.23±2.55 81.75±2.85 87.62±1.23 NS 
90 78.25±2.46 84.20±1.93 83.87±1.47 89.88±0.59 ** 
Average 80.09±1.45 84.82±1.14 83.88±1.23 87.91±0.97 ** 
LOWER IS THE DEAMINATION OF DIETARY PROTEINS HIGHER IS THE 
AVAILABILITY OF TOTAL-N FOR MICROBIAL PROTEIN SYNTHESIS
PROTEASE 
(μg hydrolyzed azocaesin) conc. 
ENZYME Control Ruchamax 
(@10g/day) 
RUCHAMAX 
N 
(@10g/day) 
OPTIRUM(@ 
10g/day) 
HIGHER IS THE PROTEASE ACTIVITY, EFFICIENT IS THE 
FERMENTATION OF RUMEN AVAILABLE PROTEIN SUBSTRATE & 
GREATER IS THE MICROBIAL PROTEIN SYNTHESIS 
significa 
nce 
Protease 
activity (μg 
hydrolyzed 
azocasein/ml/ 
min) 
0.95±0.11 
ab 
0.94±0.10 ab 0.72±0.13 a 1.26±0.10 b 0.11
Ayurvet offers 
: HERBAL HERBAL RUMEN DIGESTIVE FUNCTION TONIC & MODULATOR 
APPETIZER 
PRODUCT PRODUCT : RUCHAMAX 
: OPTIRUM 
A natural 
alternative 
A natural 
for enhancing 
alternative for 
improving animal 
fibre 
digestibility & 
productivity 
by rumen 
nutrient 
utilization SCIENTIFIC VALIDATIONS RUCHAMAX : 29, OPTIRUM: 7
Pharmacological Activity 
Zingiber officinale 
Trigonella foenum graecum 
Andrographis paniculata 
Phyllanthus emblica 
m 
a 
j 
o 
r 
c 
o 
n 
s 
t 
i 
t 
u 
e 
n 
t 
h 
e 
r 
b 
s 
Terminalia belerica
 Adaptogenic 
 Antioxidant 
 Anti-methanogenic 
REFERENCES: 
• Patra, A.K. / Kamra, D.N. / Agarwal, N. , “Effect of spices on rumen 
fermentation, methanogenesis and protozoa counts in in vitro gas production 
test”, International Congress Series, 1293, p.176-179, Jul 2006. 
• Garcia-Gonzalez, R. / Lopez, S. / Fernandez, M. / Bodas, R. / Gonzalez, J.S. , 
“Screening the activity of plants and spices for decreasing ruminal methane 
production in vitro”, Animal Feed Science and Technology, 147 (1), p.36-52, 
Nov 2008.
 For Rumen Liquor concentration 
 Promoting rumen fermentation 
 Antimethanogenic 
REFERENCES: 
Garcia-Gonzalez, R. / Lopez, S. / Fernandez, M. / Bodas, R. / Gonzalez, J.S. , “Screening the activity of plants and 
spices for decreasing ruminal methane production in vitro”, Animal Feed Science and Technology, 147 (1), p.36- 
52, Nov 2008 
Goel, G. / Makkar, H.P.S. / Becker, K. , “Effects of Sesbania sesban and Carduus pycnocephalus leaves and 
Fenugreek (Trigonella foenum-graecum L.) seeds and their extracts on partitioning of nutrients from roughage-and 
concentrate-based feeds to methane “, Animal Feed Science and Technology, 147 (1), p.72-89, Nov 2008 
Goel, G / Makkar, H P S / Becker, K , “Changes in microbial community structure, methanogenesis and rumen 
fermentation in response to saponin-rich fractions from different plant materials”, Journal of applied 
microbiology, 105 (3), p.770-777, Sep 2008
Other salient findings : 
• In untreated control group methane emission 
(l)/kg of DDMI was 66.25 lit. 
• In contrast, a significant reduction by 17.35 % 
was recorded in Ruchamax supplemented group 
with methane emission (l)/kg of DDMI was of 
54.75 L. 
• In group supplemented with AV/LMP/10, mean 
methane emission (l)/kg of DDMI of 58.21L was 
recorded which was 12.13% lesser. 
• In group supplemented with AV/AGP/10, mean 
methane emission (l)/kg of DDMI of 60.73L was 
recorded which was 8.33% lesser. 
Reference : Efficacy of herbal formulation on digestibility, nutrient utilization & methane mitigation in sheep” 
Central Research Institute for Dryland Agriculture (CRIDA), Indian Council of Agricultural Research, Santosh Nagar, 
Hyderabad, A.P. -500 059 (India)
salient findings OF 
QUBEC, CANADA TRIAL 
• In vitro trial 
• Optirum is efficacious in reducing 
NH3 conc. In RL thus inhibits 
protein degradation. 
• Optirum possesses methane 
mitigating propertiesand lowers 
NH3 production from livestock
5.2 
5 
4.8 
4.6 
4.4 
4.2 
4 
3.8 
Effect of Ruchamax supplementation 
control Ruchamax 5.1 
4.78 
4.66 4.64 4.66 
4.36 
0 15 30 
Days 
Milk yield 
There is 17 % 
increase in milk 
yield on day 30th , 
whereas milk 
yield remains 
constant in control 
group 
for 7 days on milk yield (Kg/day) 
EFFECT OF HERBAL DIGESTIVE TONIC ON MILK PRODUCTION OF BUFFALOES Indian J. Anim. Nutr., 18(3): 278-281, 2001
Effect of Ruchamax supplementation 
for 7 days on milk yield (Kg/day) 
 10 lactating cows in their 1st-6th lactation were 
divided into 2 groups of 5 animals each. 
 T1 – Control 
 T2 – Ruchamax @ 30 g per day orally 
for 15 consecutive days in a 
month for 3 months 
Bhatt et al., 2009 . Effect of Feeding Herbal Preparations on Milk Yield and Rumen 
Parameters in Lactating Crossbred Cows. International Journal of Agriculture and 
Biology, 721-726
PARAMETERS STUDIED 
 Milk Yield and milk composition 
 pH of the Rumen liquor 
 Total Bacterial count in rumen liquor 
 Total Protozoal count in rumen liquor. 
 Total volatile fatty acids.
MILK YIELD (KG/DAY) 
16 
14 
12 
10 
8 
6 
4 
2 
0 
16.13 
CONTROL RUCHAMAX 
Milk yield (Kg/day) 
There is 
52 % increase in 
milk yield in 
RUCHAMAX 
treated group 
at the end of 3 
months 
10.64
ECONOMICS OF MILK PRODUCTION 
ON RUCHAMAX FEEDING/DAY 
Parameter control Ruchamax 
Milk yield (L/day) 10.64 16.13 
Cost of milk (Rs/L) (@Rs. 35/kg) Rs. 372.40 564.55 
Feed consumed (Kg/day) 12.77 16.75 
Cost of feed @ Rs 25/Kg 319.25 418.75 
Roughage consumption (Kg/day) 8.25 11.17 
Cost of roughage @ Rs 5/Kg) Rs. 41.25 56.35 
Labour cost (Rs/day) 5 5 
Cost of product administration 
(Rs/day) 
CBR =4.17: 1 
-- 15 
Income (Rs) 6.90 69.45 
Net Profit over control (Rs) -- 62.55
RUCHAMAX IMPROVES RUCHAM FAIXBREस Dे IफGाEइSबTIरO Nपाचन में सुधार 
• बेहतर फाइबर 
पाचनशक्तत से 
पोषक तत्वों का 
अधधकतम 
उपयोग होता है! 
EFFICIENT IS THE FIBRE 
DIGESTIBILITY BETTER IS 
THE NUTRIENT 
UTILIZATION
Better is the digestibility बेहतर पाचनशक्तत, अ moधrधe isक mi lkद prधूod ucउtioत्nपादन 
फ़ीड additives क़ी पूरकता पाचनशक्तत को बेहतर बनाता है
सुरक्षित वातावरण !!!
Strategies for Poultry & Pigs 
 Nitrogen management 
Herbal Amino acids 
Herbal feed additives 
 Phosphorus 
management 
Available P 
Phytate-P
RECENT TRENDS IN CHANGING 
FEEDING STRATEGY 
This advented of 
ENVIRONMENTAL 
PROBLEMS due to excessive use 
of synthetic protein/amino acid 
sources in feed leading to pollution 
of nitrogen (N) from polutry 
droppings. 
EARLIER CONCEPT : 
dietary adjustments aiming 
to maximizing production 
performance without special 
concern for nutrient 
oversupply, especially protein 
and amino acids. 
NEW PERCEPTION : 
management of protein feeding in poultry and pigs necessitates 
nowadays a close adjustment of protein/amino acid supply to the 
requirements so as to obtain the lowest level N output by emphasizing 
more upon scientific research to search options for synthetic replacers.
NITROGEN RETENTION 
Parameters Control 
T0 
T1 
Superliv conc. Premix 
@ 500 /tonne of feed 
Nitrogen intake/bird/day/gm 5.18 5.29 
Nitrogen excreted/bird/day/gm 2.21 1.86 
Nitrogen retained/bird/day/gm 2.97 3.43 
NITROGEN (%) retained 57.18a 64.75b 
7.57% INCREASE IN NITROGEN RETENTION IN TREATMENT AS COMPARED TO CONTROL 
EFFECT OF DIFFERENT LEVELS OF HERBAL GROWTH PROMOTERS ON BROILER CHICKS. M.vsCTHESIS 
SUBMITTED TO PGIVAS, Akola, Maharashta, 2000.
Bhasker et al., Indian J. Poult. Sci. 38(2):110-114, 2002
CRUDE PROTEIN & METABOLIZABLE ENERGY RETENTION IN 
AFLATOXICATED BIRDS ADMINISTERED WITH TOXIROAK & LIVFIT VET 
GROUPS DI D2 D3 
Crude protein retention (%) 77.24a 79.64b 80.10b 
Metabolizable energy 
retention (%) 
83.10a 82.02a 82.72a 
Protein 
retention 
decreases 
in 
aflatoxicos 
•PROTEIN RETENTION is 2.4% higher in aflatoxicated birds administered Toxiroak & LivFit 
Vet@750g/ton of feed 
•PROTEIN RETENTION is 2.86% higher in aflatoxicated birds administered Toxiroak & 
LivFit Vet@500g/ton of feed 
•No adverse effect of aflatoxicosis on metabolizable energy retention could be detected 
in D2 & D3 Birds administered with herbal toxin binder & herbal liver tonic formula
EFFECT OF DIETARY ADDITION OF HERBAL GROWTH PROMOTERS ON THE PERFORMANCE OF 
BROILERS M.VSc thesis submitted to GBPUAT Pantnagar 
SUPERLIV CONC PREMIX@ 500gm/tonne of feed 
www.themegallery.com 
1.49% 
increase 
in CP 
Retention 
7.92% 
increase in 
Metabolizable 
Energy 
Retention 
8.13% 
increase in 
Retention of 
Ether Extract 
MVSc thesis submitted to GBPUAT Pantnagar)
Scientific Validations 
Publications 
Trial Reports 
Thesis 
30 
13 
10
Non-antibiotic 
Growth Promoter Natural & Safe 
Zero 
Harmful-Chemical 
Residues
Manufactured through 
superior technology 
Active herbal 
constituents helps in 
proper utilization of 
feed nutrients & 
increases the 
digestibility of feed 
which ultimately helps 
in body weight gain 
Egg production and carcass 
yield of bird increases which 
ultimately boost the economic 
importance of the Superliv 
Helps in protein retention, Metabolic energy utilization , 
Calcium & phosphorus utilization
Dietary Protein adjustments with amino acid 
supplementation 
Cicer 
Methiorep 
Balanced 
Natural Solution for synthetic Replacement 
in 
Synergy 
Phaseolus 
Triticum 
Methiorep 
Phaseolus 
Many More 
Mucuna 
Allium 
Triticum
Efficient fat & protein metabolism, lower N excretion 
Proximate Analysis (Fillet) Control Treated 
Moisture % 70 66 
Protein % 55 60 
Fat% 7.46 6.28 
Herbal Phytoaddtive Methiorep improves quality of meat and 
optimized protein and fat content of microwave cooked meat. 
Reference: Waskar, et al., 2010. Effect of 
Phytoadditive Methiorep on Carcass and Cooked 
Meat Quality Attributes in Chicken. Internet 
Journal of Veterinary Medicine, 2010, Volume 8 
No.1
Methionine Supplementation Options in Poultry. V.H. 
Kalbande, K. Ravikanth, S. Maini and D.S. Rekhe 
International Journal of Poultry Science 8 (6): 588-591, 2009. 
Effect of phytoadditive Methiorep on carcass and 
cooked meat qualities of chicken Internet Journal of 
Veterinary Medicine, 2010, Vol.8 No.1.
OTHER HERBAL AMINO ACID SUPPLEMENTS 
 REPCHOL 
 AV/CAP/18 
 AV/NLP/18
ENHANCING PHOSPHORUS UTILIZATION IN 
POULTRY & PIGS
Mean Serum Calcium, Phosphorus and Total Proteins in broilers (3rd & 
10 
8 
6 
4 
2 
0 
5th weeks) after Treatment 
19.5 % 
3rd week 5th week 3rd week 5th week 3rd week 5th week 
higher 
A B C 
7.68 
8.67 
8.2 
9.3 
7.96 
8.99 
3.97 4.1 4.23 
4.9 
4.12 
4.63 
6.63 
7.18 7.15 
7.94 
6.94 
7.72 
mg/dl 
Treatment Groups 
Serum Calcium Serum Phosphorus Total Protein 
serum phos 
phorus level 
in Ayucal D 
supplement 
ed gorup 
Serum calcium phosphorus and total proteins utilization was improved in Ayucal liquid treated 
group B as compared to untreated control group A and Ayucal –D treated group C. 
REFERENCE TRIAL REPORT “EVALUATION OF AYUCAL LIQUID AND AYUCAL-D IN IMPROVING GROWTH, PERFORMANCE AND CARCASS QUALITY IN BROILERS”” 
SUBMITTED BY DR. N.V. JADHAV, DEPT OF POULTRY SCI., BIDAR, KARNATAKA, 2009.
Nutrition and the Environment 
• Phosphorus excretion can be reduced by use of phytase 
enzymes and more closely adhering to minimum phosphorus 
needs. About 80-85% of US broiler diets use phytase 
Ayufytase 5000 
is combination of phytase 
and synergistic herbs 
which effectively breaks 
phytate bonds to release phytate 
phosphorus. 
Further the herbs present in Ayufytase 
5000 will help in effective absorption & 
utilization of the released phosphorus.
REDUCING LITTER MOISTURE 
Weaning diarrhoea in pigs 
Loose droppings in poultry 
DIAROAK is a scientific blend of herbal extracts that maintains and restores the Gastro Intestinal ( GI ) function. The ingredients of DIAROAK contains mucopolysacchrides that 
prevents the loss of water and nutrients , Increases the transit time of ingesta in gut. Adsorb & inactivate enterotoxins. Captures bacteria irreversibly
Use of Pesticides lead to destruction of ecosystems
HERBAL ECTOPARASITICIDAL & FLY REPELLANT 
“KEETGUARD LIQUID” 
INDICATIONS: 
Prevention & treatment of parasitic 
infestation by mange, mites, ticks, 
fleas, lice in livestock & poultry. 
Repelling mosquitoes, insects, fleas & 
flies commonly encountered in animal 
premises.
NONTOXIC 
NO RESIDUAL EFFECTS 
ENVIRONMENT FRIENDLY 
SAFE TO LIVESTOCK 
SAFE FOR HUMAN BEINGS
CONCLUSION
SAFE SCIENTIFIC & SUSTAINABLE APPROACH 
• Pro-active principle that scientifically 
integrates : 
Sustainabilitysustainability 
• Environmental Health 
• Economic Profitability 
• Social & Economic equity 
Source: Center for Sustainable Systems School of Natural Resources and Environment University of 
Michigan, http://css.snre.umich.edu
Ayurvet’s commitment is for the 
enhancement of health index… 
…through eco-friendly herbal formulation for 
safe animal produce
OPENING A NEW PATH FOR : 
SUSTAINABLE ANIMAL PRODUCTION & ENVIRONMENT PROTECTION 
Food security & GHG mitigation 
Thank You ! 
Questions Appreciated 
For any queries please 
contact at Email: 
shivi@ayurvet.in 
clinical@ayurvet.in 
…….THANK YOU 
This is an Ayurvet R&D presentation

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Dr.shivi maini

  • 1. TECHNICAL SESSION - II MITIGATION OF GREEN HOUSE GASES AND PROFITABLE ANIMAL FARMING
  • 2. Topic of presentation IMPACT OF USAGE OF AYURVET HERBAL SPECIALITIES TO MEET CHALLENGES OF CLIMATIC CHANGE
  • 3. CONTENTS 1 INTRODUCTION TO CLIMATE CHANGE AND GW GLOBAL GHG MITIGATION STRATEGIES www.themegallery.com 2 3 PROFITABLE ANIMAL FARMING WITH HERBAL SPECIALITIES & MEETING CHALLENGES OF CLIMATE CHANGE
  • 4. H
  • 5. (2090-2099 relative to 1980-1999) 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7 7.5 Continued emissions would lead to further warming of 1.4oC to 5.8oC over the 21st century Reference: UNFCCC, 2005 (oC) Implications of climate change :
  • 6. Mean sea level is expected to rise by 9-88 cm by 2100, causing floods, shift of climatic zones & other impacts Reference : FAO, Livestock long shadow report, (2006)
  • 7. Expected Impacts on Agriculture by 2020-2080  30% by 2050 in Central and South Asia  30% by 2080 in Latin America  50% by 2020 in some African countries 8 Possible yield reduction in agriculture: Slide adapted from Dr. R K Pachauri ‘s presentation, State of the World Symposium, Washington, 15th January 2009.
  • 8.  GW & ITS IMPACT COULD WELL DENY PEOPLE TO ACCESS THE BASIC NECESSITIES.  RISK EMANATING FROM GHGS IS NO LONGER A MATTER OF SPECULATION CH4 NO2 CO2 OZONE WATER VAPOR S CFC
  • 9. Global anthropogenic GHG emissions (2009) Sources: IPCC (2007) AR4 WGI & WGIII Greenhouse Gas Principal Sources (and Sinks) GWP* Carbon Dioxide (CO2) Fossil fuel use, land use change (oceans, terrestrial biosphere) 1 Methane (CH4) Fossil fuel mining/distribution, livestock, rice agriculture, landfills 21 Nitrous Oxide (N2O) Agriculture and associated land use change 310 “F-gases” Hydrofluorocarbons (HFCs), Perfluorocarbons (PFCs), Sulphur Hexafluoride (SF6) Industrial processes 140 - 23,900 *GWP = Global Warming Potential from Second Assessment Report, as used for reporting purposes under the UNFCCC 10
  • 10. GLOBAL WARIMNG AND CLIMATE CHANGE CONTRIBUTION OF LIVESTOCK CONTRIBUTION OF SWINE CONTRIBUTION OF POULTRY
  • 12.
  • 13. Deforest ation & desertifi cation, 35% Manure, 30% Artificial fertiliser s 3-4% Enteric fermenta tion, 25% Other 4% Animal agriculture is responsible for 18% of global greenhouse gas emissions: Methane (CH4) Nitrous Oxide (N2O) Carbon dioxide (CO2)
  • 14. Livestock industry Emissions: The single largest source of methane & nitrous oxide 37% of global methane emissions - 23x more potent than CO2 over 100 years - 68x more potent than CO2 over 20 years 65% of global nitrous oxide emissions - 297x more potent than CO2 64% of the world’s ammonia - contributing to acid rain - affecting biodiversity 9% of total CO2 emissions Source: - “Livestock’s Long Shadow Rome 2006 ” (PDF). Ch. 3, P. 82, 112, 114. Retrieved on 17-01-2009. http://tinyurl.com/96csxh
  • 15. GLOBAL METHANE EMISSIONS FROM ENTERIC FERMENTATION Emissions (million tonnes CH4 per year by source) Region/country Dairy cattle Other cattle Buffaloes Sheep and goats Pigs Total Sub-Saharan Africa 2.30 7.47 0.00 1.82 0.02 11.61 Asia * 0.84 3.83 2.40 0.88 0.07 8.02 India 1.70 3.94 5.25 0.91 0.01 11.82 China 0.49 5.12 1.25 1.51 0.48 8.85 Central and South America 3.36 17.09 0.06 0.58 0.08 21.17 West Asia & North Africa 0.98 1.16 0.24 1.20 0.00 3.58 North America 1.02 3.85 0.00 0.06 0.11 5.05 Western Europe 2.19 2.31 0.01 0.98 0.20 5.70 Oceania and Japan 0.71 1.80 0.00 0.73 0.02 3.26 Eastern Europe and CIS 1.99 2.96 0.02 0.59 0.10 5.66 Other developed 0.11 0.62 0.00 0.18 0.00 0.91 Total 15.69 50.16 9.23 9.44 1.11 85.63 Source: FAO (2007)
  • 16. 100 million tonnes of methane each year SOURCE: GHG online, Methane, sources, sinks & science
  • 17. N O EMISSIONS FROM LIVESTOCK (million tonnes per year) 2 Region/country Dairy cattle Other cattle Buffalo Sheep and goats Pigs Poultry Total Sub-Saharan Africa 0.06 0.21 0.00 0.13 0.01 0.02 0.43 Asia * 0.02 0.14 0.06 0.05 0.03 0.05 0.36 India 0.03 0.15 0.06 0.05 0.01 0.01 0.32 China 0.01 0.14 0.03 0.10 0.19 0.10 0.58 Central and South 0.08 0.41 0.00 0.04 0.04 0.05 0.61 America West Asia and North Africa 0.02 0.03 0.00 0.09 0.00 0.03 0.17 North America 0.03 0.20 0.00 0.00 0.04 0.04 0.30 Western Europe 0.06 0.14 0.00 0.07 0.07 0.03 0.36 Oceania and Japan 0.02 0.08 0.00 0.09 0.01 0.01 0.21 Eastern Europe and CIS 0.08 0.08 0.00 0.03 0.04 0.02 0.28 Other developed 0.00 0.03 0.00 0.02 0.00 0.00 0.06 Total 0.41 1.64 0.15 0.68 0.44 0.36 3.69 Source: FAO (2007)
  • 18. Relative emission of GHG from different meat types COMMODITY C0% CH4 % N2O % Pig meat 47 22 53 Chicken 45 2 58 Beef 21 49 31 Sheep meat/ Chevon 19 50 31 Milk 24 49 27 Eggs 46 4 50 Reference: Williams, a.g., Adsley, E., Sandras, D.L. Determining the environmental burdens and resource use in the production of agriculture and horticulture commodities. Defra Research report, 2007. The FAO report states that current production levels of meat contribute between 14 and 22 percent of the 36 billion tons of "CO2-equivalent" greenhouse gases the world produces every year. - Scientific American, Feb, 2009
  • 19. Meat consumption is expected to double by 2050  Raising animals for food is a major cause of global warming and yet the global livestock sector is growing faster than any other agricultural sub-sector.  Dairy output is expected to more than double by 2050.  Animal Protein vs. Plant protein Source: -UN FAO, Livestock’s Long Shadow, Rome 2006 -The livestock industry and climate – EU makes bad worse, Jens Holm & Toivo Jokkala, Parliamentary Group (p. 17-18) livestock industry FUTURE OUTLOOK
  • 20. “Livestock’s contribution to environmental problems is on a massive scale and its potential contribution to their solution is equally large. The impact is so significant that it needs to be addressed with urgency.” - U.N. Food & Agriculture Organization, 2006
  • 21. The fastest way to slow global warming and curb climate change? Cut down on short-lived greenhouse gases such as Methane – the single largest source of which is livestock.
  • 22.  Methane is one of the 6 GHGs identified under United Nations Framework Convention on Climatic Change UNFCCC Kyoto Protocol .This GHG has 21 times more GWP than CO 2  Methane is more than 21 times as effectual as CO2 at entrapping heat in the atmosphere.  A shorter chemical half life of 12 yrs as compared to 120 yrs of CO 2  Even if the entire world switched to a zero carbon economy and lifestyle tomorrow, it would take 1000’s years for CO2 to dissipate out of the atmosphere.  Cutting down on short-lived GHGs will quickly translate into cooling of the Earth which will give us time to deal with CO2 emissions. Source: IPCC 4th Assessment, IPCC Fourth Assessment Report – Synthesis Report 2007 http://www.ipcc.ch/ipccreports/ar4-syr.htm, chapter 2 http://ipcc-wg1.ucar.edu/wg1/wg1-report.html
  • 23. RUMEN 80-95% www.themegallery.com INTESTINE 5-20 % Methane produced/day/ animal: 250-500 lit
  • 24. ORGANIC MATTER BACTERIA PROTOZOA FUNGI ATP H2 VFA CO2 CH4 www.themegallery.com CO 2 + 4H 2 → CH 4 + 2H O 2
  • 25.
  • 26. US AGENCIES BROUGHT HIGH LEVEL FOCUS ON METHANE Supporting projects in Argentina, Brazil, China, Colombia, Ecuador, India, Korea, Mexico, Mongolia, Nigeria, Poland, Philippines, Russia, Thailand, Ukraine and Vietnam. Provides technical assistance and capacity building to ensure long-term project success of projects BY Successful engagement of key developing countries
  • 27. • Some cattle and sheep naturally Flatulence Vaccine produce less methane. Efforts are underway Another area to of determine study has the been gene the or mapping genes involved. of the methane Farmers genome, could which then a breed New Zealand for lower research greenhouse organization has gas producing accomplished. livestock. With this knowledge As with they the will flatulence work toward vaccine, producing ensuring a vaccine that to prevent belching and flatulence. Vaccine new development problems and are refinement not created may take in some the effort time. to genetically manage existing ones will be key to taking advantage of this inherited trait. • Source:http://www.suite101.com/content/managing-methane- from-livestock-a58094#ixzz1IB2E9vOO SOURCE: http://www.suite101.com/content/managing-methane- from-livestock-a58094#ixzz1IB3XyljY A RESERCH IN NEWZEALAND The 45 million sheep and 10 million cattle in New Zealand burped and farted about 90 percent of that country's methane emissions, according to government figures.
  • 28. Argentina has more than 55 million cows, making it a leading producer of beef. In the study, the scientists were surprised to discover that a standard 550-kg cow produces between 800 to 1,000 liters of emissions, including methane, each day. PhysOrg.com) -- In an attempt to understand the extent of cow flatulence on global warming, scientists in Argentina are strapping plastic bags to the backs of cows to capture their emissions.
  • 29. 'Burpless' Grass Cuts Methane Gas From Cattle, May Help Reduce Global Warming • Scientists in a joint biotech venture by Australia’s Molecular Plant Breeding Cooperative Research Centre and New Zealand rural services group PGG Wrightson Genomics, are developing a grass that will not only cut the amount of methane cows burp up when chewing the cud but also grow in hotter climes. Grass that may help tackle global warming by cutting the level of methane given off by cows is in the works. (Credit: USDA) Ref: Science Daily, May 8, 2010 • Scientists in a joint biotech venture by Australia’s Molecular Plant Breeding Cooperative Research Centre and New Zealand rural services group PGG Wrightson Genomics, are developing a grass that will not only cut the amount of methane cows burp up when chewing the cud but also grow in hotter climes.
  • 30. Unusual Feed Supplement Could Ease Gassy Cows, Reduce Their Greenhouse Gas Emissions • In a series of laboratory experiments conducted by Penn State Dairy Scientists, and in a live animal test, an oregano-based supplement not only decreased methane emissions in dairy cows by 40 percent, but also improved milk production, according to Alexander Hristov, an associate professor of dairy nutrition. Cow belches, a major source of greenhouse gases, could be decreased by an unusual feed supplement developed by a Penn State dairy scientist. (Credit: iStockphoto/Fabian Rothe) Ref: Science Daily, Sept 8, 2010
  • 31. Fish Oils Reduce Greenhouse Gas Emissions From Flatulent Cows • The benefits to animals of omega 3 fatty acids in fish oils have been well documented – helping the heart and circulatory system, improving meat quality and reducing methane emissions. Researchers from University College Dublin reported that by including 2% fish oil in the diet of cattle, they achieved a reduction in the amount of methane released by the animals. New research shows that fish oils can improve meat quality and reducing methane emissions in cows. (Credit: iStockphoto) ScienceDaily (Mar. 30, 2009)
  • 32. 45 40 35 30 25 20 15 10 5 0 BIOLOGICAL CONTROL VACCINATION HERD MANAGEMENT Low Medium High Mitigation % DIETARY SUPPLEMENTS BREEDING BMPS RUMEN MANIPULATION Immediate Longer Term Eckard, Grainger & de Klein 2010 Likely Impact
  • 33.
  • 34. 1. Studies on methane mitigation in goats fed roughage based diets by supplementing polyherbal products, thesis submitted to Durg veterinary college, Durg, Chattisgarh, 2012. 2. Effects of herbs, spices and essential oils and their combination on rumen microbial fermentation and methane production in vitro. Chaouki Benchaar, Ph.D. Agriculture and Agri-Food Canada Dairy and Swine Research and Development Centre Sherbrooke, Quebec, Canada JIM 0C8, 2013. 3. Efficacy of herbal formulation on digestibility, nutrient utilization & methane mitigation in sheep” submitted by Dr. Prabhat Kr. Pankaj, ARS, Senior Scientist, Central Research Institute for Dryland Agriculture (CRIDA), Indian Council of Agricultural Research, Santosh Nagar, Hyderabad, A.P. -500 059 (India), 2014.
  • 35.
  • 36. Thesis Submitted by: Dr. Subhanker Nanda GUIDE: DR. S.P.TIWARI PROFESSOR & HEAD, DEPT. OF ANIMAL NUTRITION COLLEGE OF VETERINARY & ANIMAL SCIENMCES, ANJORA, DURG, CHATTISGARH, PIN: 491001 www.themegallery.com Thesis
  • 37. PARTICULARS TREATMENTS T0 T1 T2 T3 No. of Animal 6 6 6 6 Basal Diet Concentrate Roughage 250 g ad libitum 250g ad libitum 250g ad libitum 250g ad libitum Polyherbal Product (0-21 days; 30-51 days 60-81 days) - Ruchamax (@10gm/day) AV/DAC/16 (@10gm/day) Optirum (@10gm/day) Reference : Studies on methane mitigation in goats fed roughage based diet & its mitigation by supplementing polyherbal products . Thesis submitted to Durg Veterinary College, Chattisgarh, India, 2012. www.themegallery.com
  • 38. RUMINAL ENZYMES PROTOZOAL COUNT & RUMINALMOTILITY GROWTH PERFORMANCE, DIGESTIBILITY & METABOLIC RUMEN FERMENTATION PATTERN & BIOCHEMICAL PARAMTERES www.themegallery.com STUDIES DRY MATTER INTAKE & METHANE PRODUCTION FEED ANALYSIS
  • 39. in vitro studies Particulars Treatments T0 T1 T2 T3 Syringe (no.) 4 4 4 4 Feed substrate (basal diet) 0.2g 0.2g 0.2g 0.2g Anaerobic medium (SRL) 30ml 30ml 30ml 30ml Polyherbal product - Ruchamax @0.002g/day Ruchamax @0.002g/day Optirum @0.002g/day The in vitro gas production test was performed as per Menke and Steingass (1988). Estimation of methane was carried out in Nucon-5765 gas chromatograph equipped with a stainless still column packed with Porapak-Q. Studies on methane mitigation in goats fed roughage based diets by supplementing polyherbal products, thesis submitted to Durg veterinary college, Durg, Chattisgarh, 2012.
  • 40. In vitro Trial photos
  • 41. IN VITRO METHANE GAS PRODUCTION (ml/g DM) 50 55 60 65 70 Optirum Ruchamax N Ruchamax Control 69.29 61.76 67.51 59.64 Methane (ml/g DM) OPTIRUM: 13.92% reduction in Methane production RUCHAMAX : 10.86 % reduction in Methane production RUCHAMAX N: 2.56 % reduction in Methane production
  • 42. OMD (%) 30.9 32.36 30.37 36.67 40 30 20 10 0 Control Ruchamax Ruchamax N Optirum In vitro organic matter digestibility is higher in treated group than control, higher is the digestibility lower is the production of methane •in vitro gas production test was performed as per Menke and Steingass (1988).
  • 43. 4.1 5.8 5.05 6.05 8 6 4 2 0 Control Ruchamax Ruchamax N Optirum T V F A DAY 21 4.23 5.72 5.13 6.12 8 6 4 2 0 Control Ruchamax Ruchamax N Optirum DAY 45 4.15 DAY 90 Increase in TVFA concentration in 5.85 5.39 6.08 8 6 4 2 0 Control Ruchamax Ruchamax N Optirum treated groups might be due to significant increase in fermentation of carbohydrates and simultaneously more shifting of methane energy toward the production of propionic acid. 46.5% INCREASE
  • 44. 66 64 62 60 58 61.45 61.69 63.73 65.05 DAY 21st 65 62.9 64 63 62 61 DAY 45th 64.87 63.21 63.43 70 65 60 55 67.9 65.04 62.37 60.58 90 POOR IS THE FIBRE DIGESTION HIGHER IS THE CONCENTRATION OF ACETATE & MORE IS THE METHANE PRODUCTION Day45th: ns Day90th : s 10.78% DECREASE
  • 45. PERIOD (days) GROUPS CONTROL RUCHAMAX RUCHAMAX N OPTIRUM SIGNIFICANCE 21 15.67±0.49 16.35±0.18 16.63±0.07 16.38±0.08 NS 45 16.17±0.08 a 17.03±0.20 bc 16.53±0.17 ab 17.50±0.3 d ** 90 16.53±0.18 a 17.50±0.22 a 17.52±0.39 a 19.31±0.4 b ** Average 16.12±0.19 a 16.96±0.18 b 16.89±0.17 b 17.73±0.34 c ** Better is the nutrient digestion, lower is the methane production and greater is the shifting the H+ ions for the production of useful energy in the body in the form of propionic acid.
  • 46. 3.18 Day 90th 2.43 2.11 2.47 3.5 3 2.5 2 1.5 1 0.5 0 Control Ruchamax Ruchamax N Optirum There was a significant reduction of protozoal population by 22.3% in T3 and 23.65% in T2 (Ruchamax). The comparative suppression of the protozoal population might have reduced the methane production in these groups.
  • 47. HOLOTRICHS (% IN RL) 69 68 67 66 65 64 63 62 61 60 59 Control Ruchamax Ruchamax N Optirum 68.61 62.94 66.67 62.28 holotrichs Holotrichs are higher in the fibrous and sugar based diet. In the present study Holotrichs were higher in control due to poor fibre digestibility The reduction in protozoal numbers in treatments groups were due to presence of defaunating principles of the herbs. 9.22% DECREASE
  • 48. • PROTOZOA • BACTERIA DEFAUNATION BIOCHEMICAL MODULATION • Xylanse • TVFA, APB • TOTAL-N • NH3-N2 • Protease ENZYMATIC MODULATION
  • 49. PARAMETERS NUTRIENT BALANCE (%) CONTROL RUCHAMAX RUCHAMAX N OPTIRUM NITROGEN BALANCE 62.79±1.95 64.38±1.86 63.37±1.05 67.54±1.88 CALCIUM BALANCE 68.5±4.65 69.8±4.85 69.12±4.75 70.1±4.58 PHOSPHORUS BALANCE 72.4±2.58 76.5±2.15 73.5±1.65 78.5±2.01  the percent N balance (%) & Phosphorus balance was significantly higher in group T3 and T1 over control  the percent Ca balance (%) & Phosphorus balance was significantly higher in group T3 over control, T1 & T2were non-significantly different
  • 50. PERIOD (days) GROUPS CONTROL RUCHAMAX RUCHAMAX N OPTIRUM SIGNIFICANCE 21 79.75±3.31 84.03±1.62 83.03±2.18 86.23±2.55 NS 45 82.28±1.75 86.23±2.55 81.75±2.85 87.62±1.23 NS 90 78.25±2.46 84.20±1.93 83.87±1.47 89.88±0.59 ** Average 80.09±1.45 84.82±1.14 83.88±1.23 87.91±0.97 ** LOWER IS THE DEAMINATION OF DIETARY PROTEINS HIGHER IS THE AVAILABILITY OF TOTAL-N FOR MICROBIAL PROTEIN SYNTHESIS
  • 51. PROTEASE (μg hydrolyzed azocaesin) conc. ENZYME Control Ruchamax (@10g/day) RUCHAMAX N (@10g/day) OPTIRUM(@ 10g/day) HIGHER IS THE PROTEASE ACTIVITY, EFFICIENT IS THE FERMENTATION OF RUMEN AVAILABLE PROTEIN SUBSTRATE & GREATER IS THE MICROBIAL PROTEIN SYNTHESIS significa nce Protease activity (μg hydrolyzed azocasein/ml/ min) 0.95±0.11 ab 0.94±0.10 ab 0.72±0.13 a 1.26±0.10 b 0.11
  • 52. Ayurvet offers : HERBAL HERBAL RUMEN DIGESTIVE FUNCTION TONIC & MODULATOR APPETIZER PRODUCT PRODUCT : RUCHAMAX : OPTIRUM A natural alternative A natural for enhancing alternative for improving animal fibre digestibility & productivity by rumen nutrient utilization SCIENTIFIC VALIDATIONS RUCHAMAX : 29, OPTIRUM: 7
  • 53. Pharmacological Activity Zingiber officinale Trigonella foenum graecum Andrographis paniculata Phyllanthus emblica m a j o r c o n s t i t u e n t h e r b s Terminalia belerica
  • 54.  Adaptogenic  Antioxidant  Anti-methanogenic REFERENCES: • Patra, A.K. / Kamra, D.N. / Agarwal, N. , “Effect of spices on rumen fermentation, methanogenesis and protozoa counts in in vitro gas production test”, International Congress Series, 1293, p.176-179, Jul 2006. • Garcia-Gonzalez, R. / Lopez, S. / Fernandez, M. / Bodas, R. / Gonzalez, J.S. , “Screening the activity of plants and spices for decreasing ruminal methane production in vitro”, Animal Feed Science and Technology, 147 (1), p.36-52, Nov 2008.
  • 55.  For Rumen Liquor concentration  Promoting rumen fermentation  Antimethanogenic REFERENCES: Garcia-Gonzalez, R. / Lopez, S. / Fernandez, M. / Bodas, R. / Gonzalez, J.S. , “Screening the activity of plants and spices for decreasing ruminal methane production in vitro”, Animal Feed Science and Technology, 147 (1), p.36- 52, Nov 2008 Goel, G. / Makkar, H.P.S. / Becker, K. , “Effects of Sesbania sesban and Carduus pycnocephalus leaves and Fenugreek (Trigonella foenum-graecum L.) seeds and their extracts on partitioning of nutrients from roughage-and concentrate-based feeds to methane “, Animal Feed Science and Technology, 147 (1), p.72-89, Nov 2008 Goel, G / Makkar, H P S / Becker, K , “Changes in microbial community structure, methanogenesis and rumen fermentation in response to saponin-rich fractions from different plant materials”, Journal of applied microbiology, 105 (3), p.770-777, Sep 2008
  • 56. Other salient findings : • In untreated control group methane emission (l)/kg of DDMI was 66.25 lit. • In contrast, a significant reduction by 17.35 % was recorded in Ruchamax supplemented group with methane emission (l)/kg of DDMI was of 54.75 L. • In group supplemented with AV/LMP/10, mean methane emission (l)/kg of DDMI of 58.21L was recorded which was 12.13% lesser. • In group supplemented with AV/AGP/10, mean methane emission (l)/kg of DDMI of 60.73L was recorded which was 8.33% lesser. Reference : Efficacy of herbal formulation on digestibility, nutrient utilization & methane mitigation in sheep” Central Research Institute for Dryland Agriculture (CRIDA), Indian Council of Agricultural Research, Santosh Nagar, Hyderabad, A.P. -500 059 (India)
  • 57. salient findings OF QUBEC, CANADA TRIAL • In vitro trial • Optirum is efficacious in reducing NH3 conc. In RL thus inhibits protein degradation. • Optirum possesses methane mitigating propertiesand lowers NH3 production from livestock
  • 58. 5.2 5 4.8 4.6 4.4 4.2 4 3.8 Effect of Ruchamax supplementation control Ruchamax 5.1 4.78 4.66 4.64 4.66 4.36 0 15 30 Days Milk yield There is 17 % increase in milk yield on day 30th , whereas milk yield remains constant in control group for 7 days on milk yield (Kg/day) EFFECT OF HERBAL DIGESTIVE TONIC ON MILK PRODUCTION OF BUFFALOES Indian J. Anim. Nutr., 18(3): 278-281, 2001
  • 59. Effect of Ruchamax supplementation for 7 days on milk yield (Kg/day)  10 lactating cows in their 1st-6th lactation were divided into 2 groups of 5 animals each.  T1 – Control  T2 – Ruchamax @ 30 g per day orally for 15 consecutive days in a month for 3 months Bhatt et al., 2009 . Effect of Feeding Herbal Preparations on Milk Yield and Rumen Parameters in Lactating Crossbred Cows. International Journal of Agriculture and Biology, 721-726
  • 60. PARAMETERS STUDIED  Milk Yield and milk composition  pH of the Rumen liquor  Total Bacterial count in rumen liquor  Total Protozoal count in rumen liquor.  Total volatile fatty acids.
  • 61. MILK YIELD (KG/DAY) 16 14 12 10 8 6 4 2 0 16.13 CONTROL RUCHAMAX Milk yield (Kg/day) There is 52 % increase in milk yield in RUCHAMAX treated group at the end of 3 months 10.64
  • 62. ECONOMICS OF MILK PRODUCTION ON RUCHAMAX FEEDING/DAY Parameter control Ruchamax Milk yield (L/day) 10.64 16.13 Cost of milk (Rs/L) (@Rs. 35/kg) Rs. 372.40 564.55 Feed consumed (Kg/day) 12.77 16.75 Cost of feed @ Rs 25/Kg 319.25 418.75 Roughage consumption (Kg/day) 8.25 11.17 Cost of roughage @ Rs 5/Kg) Rs. 41.25 56.35 Labour cost (Rs/day) 5 5 Cost of product administration (Rs/day) CBR =4.17: 1 -- 15 Income (Rs) 6.90 69.45 Net Profit over control (Rs) -- 62.55
  • 63. RUCHAMAX IMPROVES RUCHAM FAIXBREस Dे IफGाEइSबTIरO Nपाचन में सुधार • बेहतर फाइबर पाचनशक्तत से पोषक तत्वों का अधधकतम उपयोग होता है! EFFICIENT IS THE FIBRE DIGESTIBILITY BETTER IS THE NUTRIENT UTILIZATION
  • 64. Better is the digestibility बेहतर पाचनशक्तत, अ moधrधe isक mi lkद prधूod ucउtioत्nपादन फ़ीड additives क़ी पूरकता पाचनशक्तत को बेहतर बनाता है
  • 66.
  • 67. Strategies for Poultry & Pigs  Nitrogen management Herbal Amino acids Herbal feed additives  Phosphorus management Available P Phytate-P
  • 68. RECENT TRENDS IN CHANGING FEEDING STRATEGY This advented of ENVIRONMENTAL PROBLEMS due to excessive use of synthetic protein/amino acid sources in feed leading to pollution of nitrogen (N) from polutry droppings. EARLIER CONCEPT : dietary adjustments aiming to maximizing production performance without special concern for nutrient oversupply, especially protein and amino acids. NEW PERCEPTION : management of protein feeding in poultry and pigs necessitates nowadays a close adjustment of protein/amino acid supply to the requirements so as to obtain the lowest level N output by emphasizing more upon scientific research to search options for synthetic replacers.
  • 69. NITROGEN RETENTION Parameters Control T0 T1 Superliv conc. Premix @ 500 /tonne of feed Nitrogen intake/bird/day/gm 5.18 5.29 Nitrogen excreted/bird/day/gm 2.21 1.86 Nitrogen retained/bird/day/gm 2.97 3.43 NITROGEN (%) retained 57.18a 64.75b 7.57% INCREASE IN NITROGEN RETENTION IN TREATMENT AS COMPARED TO CONTROL EFFECT OF DIFFERENT LEVELS OF HERBAL GROWTH PROMOTERS ON BROILER CHICKS. M.vsCTHESIS SUBMITTED TO PGIVAS, Akola, Maharashta, 2000.
  • 70. Bhasker et al., Indian J. Poult. Sci. 38(2):110-114, 2002
  • 71. CRUDE PROTEIN & METABOLIZABLE ENERGY RETENTION IN AFLATOXICATED BIRDS ADMINISTERED WITH TOXIROAK & LIVFIT VET GROUPS DI D2 D3 Crude protein retention (%) 77.24a 79.64b 80.10b Metabolizable energy retention (%) 83.10a 82.02a 82.72a Protein retention decreases in aflatoxicos •PROTEIN RETENTION is 2.4% higher in aflatoxicated birds administered Toxiroak & LivFit Vet@750g/ton of feed •PROTEIN RETENTION is 2.86% higher in aflatoxicated birds administered Toxiroak & LivFit Vet@500g/ton of feed •No adverse effect of aflatoxicosis on metabolizable energy retention could be detected in D2 & D3 Birds administered with herbal toxin binder & herbal liver tonic formula
  • 72. EFFECT OF DIETARY ADDITION OF HERBAL GROWTH PROMOTERS ON THE PERFORMANCE OF BROILERS M.VSc thesis submitted to GBPUAT Pantnagar SUPERLIV CONC PREMIX@ 500gm/tonne of feed www.themegallery.com 1.49% increase in CP Retention 7.92% increase in Metabolizable Energy Retention 8.13% increase in Retention of Ether Extract MVSc thesis submitted to GBPUAT Pantnagar)
  • 73. Scientific Validations Publications Trial Reports Thesis 30 13 10
  • 74. Non-antibiotic Growth Promoter Natural & Safe Zero Harmful-Chemical Residues
  • 75. Manufactured through superior technology Active herbal constituents helps in proper utilization of feed nutrients & increases the digestibility of feed which ultimately helps in body weight gain Egg production and carcass yield of bird increases which ultimately boost the economic importance of the Superliv Helps in protein retention, Metabolic energy utilization , Calcium & phosphorus utilization
  • 76. Dietary Protein adjustments with amino acid supplementation Cicer Methiorep Balanced Natural Solution for synthetic Replacement in Synergy Phaseolus Triticum Methiorep Phaseolus Many More Mucuna Allium Triticum
  • 77. Efficient fat & protein metabolism, lower N excretion Proximate Analysis (Fillet) Control Treated Moisture % 70 66 Protein % 55 60 Fat% 7.46 6.28 Herbal Phytoaddtive Methiorep improves quality of meat and optimized protein and fat content of microwave cooked meat. Reference: Waskar, et al., 2010. Effect of Phytoadditive Methiorep on Carcass and Cooked Meat Quality Attributes in Chicken. Internet Journal of Veterinary Medicine, 2010, Volume 8 No.1
  • 78. Methionine Supplementation Options in Poultry. V.H. Kalbande, K. Ravikanth, S. Maini and D.S. Rekhe International Journal of Poultry Science 8 (6): 588-591, 2009. Effect of phytoadditive Methiorep on carcass and cooked meat qualities of chicken Internet Journal of Veterinary Medicine, 2010, Vol.8 No.1.
  • 79. OTHER HERBAL AMINO ACID SUPPLEMENTS  REPCHOL  AV/CAP/18  AV/NLP/18
  • 80. ENHANCING PHOSPHORUS UTILIZATION IN POULTRY & PIGS
  • 81. Mean Serum Calcium, Phosphorus and Total Proteins in broilers (3rd & 10 8 6 4 2 0 5th weeks) after Treatment 19.5 % 3rd week 5th week 3rd week 5th week 3rd week 5th week higher A B C 7.68 8.67 8.2 9.3 7.96 8.99 3.97 4.1 4.23 4.9 4.12 4.63 6.63 7.18 7.15 7.94 6.94 7.72 mg/dl Treatment Groups Serum Calcium Serum Phosphorus Total Protein serum phos phorus level in Ayucal D supplement ed gorup Serum calcium phosphorus and total proteins utilization was improved in Ayucal liquid treated group B as compared to untreated control group A and Ayucal –D treated group C. REFERENCE TRIAL REPORT “EVALUATION OF AYUCAL LIQUID AND AYUCAL-D IN IMPROVING GROWTH, PERFORMANCE AND CARCASS QUALITY IN BROILERS”” SUBMITTED BY DR. N.V. JADHAV, DEPT OF POULTRY SCI., BIDAR, KARNATAKA, 2009.
  • 82. Nutrition and the Environment • Phosphorus excretion can be reduced by use of phytase enzymes and more closely adhering to minimum phosphorus needs. About 80-85% of US broiler diets use phytase Ayufytase 5000 is combination of phytase and synergistic herbs which effectively breaks phytate bonds to release phytate phosphorus. Further the herbs present in Ayufytase 5000 will help in effective absorption & utilization of the released phosphorus.
  • 83. REDUCING LITTER MOISTURE Weaning diarrhoea in pigs Loose droppings in poultry DIAROAK is a scientific blend of herbal extracts that maintains and restores the Gastro Intestinal ( GI ) function. The ingredients of DIAROAK contains mucopolysacchrides that prevents the loss of water and nutrients , Increases the transit time of ingesta in gut. Adsorb & inactivate enterotoxins. Captures bacteria irreversibly
  • 84. Use of Pesticides lead to destruction of ecosystems
  • 85. HERBAL ECTOPARASITICIDAL & FLY REPELLANT “KEETGUARD LIQUID” INDICATIONS: Prevention & treatment of parasitic infestation by mange, mites, ticks, fleas, lice in livestock & poultry. Repelling mosquitoes, insects, fleas & flies commonly encountered in animal premises.
  • 86. NONTOXIC NO RESIDUAL EFFECTS ENVIRONMENT FRIENDLY SAFE TO LIVESTOCK SAFE FOR HUMAN BEINGS
  • 88. SAFE SCIENTIFIC & SUSTAINABLE APPROACH • Pro-active principle that scientifically integrates : Sustainabilitysustainability • Environmental Health • Economic Profitability • Social & Economic equity Source: Center for Sustainable Systems School of Natural Resources and Environment University of Michigan, http://css.snre.umich.edu
  • 89. Ayurvet’s commitment is for the enhancement of health index… …through eco-friendly herbal formulation for safe animal produce
  • 90. OPENING A NEW PATH FOR : SUSTAINABLE ANIMAL PRODUCTION & ENVIRONMENT PROTECTION Food security & GHG mitigation Thank You ! Questions Appreciated For any queries please contact at Email: shivi@ayurvet.in clinical@ayurvet.in …….THANK YOU This is an Ayurvet R&D presentation