This document discusses the use of oxygen/ozone therapy in dentistry. It provides information on the fundamental scientific facts about oxygen/ozone, including its disinfection properties, ability to improve wound healing, activation of red blood cell metabolism and antioxidant systems, anti-inflammatory effects, and ability to increase circulation. The document outlines how ozone is created and its uses in treating dental issues like infections, periodontal disease, root canal therapy, and enhanced wound healing. Ozone therapy is presented as a safe, non-toxic alternative to antibiotics for treating dental problems and infections.

1. AMERICAN COLLEGE OF INTEGRATIVE
MEDICINE AND DENTISTRY
PRESENTS
OXYGEN/OZONE THERAPY IN
DENTISTRY

2. FUNDAMENTAL SCIENTFIC FACTS
ABOUT OXYGEN/OZONE
UNIVERSITY OF HAVANA, CUBA
DEPARTMENT OF PHARMACOLOGY
PROF. OLGA SONIA LEON FERNANDEZ
VIENNA, OCT. 8 -10, 2011
*DISINFECTION PROPERITES
*NON-TOXIC WOUND CLEANING
*IMPROVED AND ACCERATED WOUND
HEALING

3. FUNDAMENTAL SCIENTFIC
FACTS ABOUT OXYGEN/OZONE
UNIVERSITY OF HAVANA, CUBA
DEPARTMENT OF PHARMACOLOGY
PROF. OLGA SONIA LEON FERNANDEZ
VIENNA, OCT. 8 -10, 2011
+ACTIVATION OF RED BLOOD CELL METABOLISM
WITH IMPROVED OXYGEN RELEASE
+ACTIVATION OF ENZYMATIC ANTIOXIDANTS AND
RADICAL SCAVENGERS

4. FUNDAMENTAL SCIENTFIC FACTS
ABOUT OXYGEN/OZONE
*ACTIVATION OF IMMUNOCOMPETENT CELLS
AND RELEASE OF CYTOKINES SUCH AS
INTERFERONES AND INTERLEUKINS.
MODULATION OF THE IMMUNE SYSTEM
UNIVERSITY OF HAVANA, CUBA
DEPARTMENT OF PHARMACOLOGY
PROF. OLGA SONIA LEON FERNANDEZ
VIENNA, OCT. 8 -10, 2011

5. FUNDAMENTAL SCIENTFIC FACTS
ABOUT OXYGEN/OZONE
UNIVERSITY OF HAVANA, CUBA
DEPARTMENT OF PHARMACOLOGY
PROF. OLGA SONIA LEON FERNANDEZ
VIENNA, OCT. 8 -10, 2011
*INCREASED ANTIOXIDANT CAPACITY
THROUGH ACTIVATION OF CELLULAR
ANTIOXIDANT SYSTEMS
CATALASE, SUPEROXIDE DISMUTATSE
CATALASE

6. FUNDAMENTAL SCIENTFIC FACTS
ABOUT OXYGEN/OZONE
*ANTIINFLAMMATORY EFFECT
*INCREASED CIRCULATIONVIA NITRIC OXIDE
*HIGH REDOX POTENTIAL

7. WHAT
IS
OZONE ?

8. • A chemical compound consisting of three oxygen atoms (O3).
• The elemental form of oxygen occurring naturally as a result
of ultraviolet energy or lightning causing a temporary
recombination of oxygen atoms into groups of three.
• The form of oxygen that is formed by an electric discharge
field of a corona discharge generator (simulates lightning).
• Oxygen is the only gas that can accept and carry an
electrical charge. Remember, the terminal electron acceptor.

9. HOW IS OZONE CREATED?

10. OXYGEN AND A ENERGY SOURCE

11. A MEDICAL GRADE OXYGEN FED COLD CORONA DISCHARGE
OZONE UNIT

12. AND SEARCH “OZONE DENTISTRY”
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1.
Treatment of Root Canal Biofilms of Enterococcus faecalis with Ozone Gas and Passive Ultrasound Activation.
Case PD, Bird PS, Kahler WA, George R, Walsh LJ.
J Endod. 2012 Apr;38(4):523-6. Epub 2012 Feb 2.
PMID: 22414842 [PubMed - in process]
Related citations
2.
Ozone therapy in dentistry: A strategic review.
Saini R.
J Nat Sci Biol Med. 2011 Jul;2(2):151-3.
PMID: 22346227 [PubMed - in process] Free PMC Article
191 articles

13. PRIMARY ISSUES IN DENTISTRY
• TRAUMA
• INFECTION
• BACTERIAL
• FUNGAL
• VIRAL
• PARASITIC

14. These infections can be:
• Acute
• Chronic
• Bacterial
• Viral
• Fungal
• Parasitic
• OR… A some combination of the above
• And RESISTANT to current therapeutics

15. IntegrativeTreatment Regimens
• Antibiotics
• Antifungals
• Antivirals
• Antiparasitics
• Antiseptics
• Essential Oils/Plant
Extracts
• Homeopathics
• Lasers
• Ultraviolet Light
• Oxygen/Ozone
Therapies

16. All of these regimens have proven to be
effective in the treatment of infections
BUT…

17. MOST OFTHE REGIMENS HAVE
SIGNIFICANT SIDE EFFECTS
EXCEPT…

18. OXYYGEN/OZONETHERAPY

19. Multitude of resources for use in human treatment.
When used at a therapeutic level

20. DOSE AND LANGUAGE USE FOR
OZONETHERAPY IN DENTISTRY
• EXPRESSED AS MICROGRAMS PER MILLILITERS
OF OXYGEN (mcg/ml)
• ALSO EXPRESSED AS “GAMMA”
• INTERNAL USE RANGE: 6 - 30µg/ml (dependent on
application)
• EXTERNAL USE RANGE: 40- 50µg/ml (root
canal therapy & caries)
• **START with a LOW dose and GO SLOW***

21. What Treatment Regimens will
Effectively Eliminate:
• BACTERIA
• VIRUSES
• FUNGI
• PARASITES

22. 1. Bacteria
2. Ozone contacts cell membrane
3. Ozone (peroxides) penetrates cell membrane
4. Rupture of cell membrane begins
5. Membrane loses integrity
6. Bacteria lyses due to ozone

23. OZONE IS PRODUCED BY ANTIBODIES DURING BACTERIAL
KILLING IN INFLAMMATION
THE SCRIPPS RESEARCH INSTITUTE
NOVEMBER 18, 2002
LERNER,WENTWORH ET AL. SCIENCE

24. WHITE BLOOD CELLS/
PLASMA CELLS/
ANTIBODIES

25. OXIDATION /BIOFILM
The cover story in “THE JOURNAL OFTHE AMERICAN DENTAL ASSOCIATION,”
Vol 140, No 8 978-986 is “Periodontitis:An Archetypical Biofilm Disease”
It states that “Periodontitis is a classic example of biofilm-mediated diseases.”
The article concluded that,“Periodontitis, like other biofilm infections, is refractory to antibiotic
agents and host defenses because the causative microbes live in complex communities that
persist despite challenges that range from targeted antibiotic agents to phagocytosis.” The
clinical implications concluded that “The regular delivery of non targeted anti-biofilm agents
may be an effective strategy for treating biofilms, especially if these agents include oxidative
agents that dissolve the biofilm matrix.”
OZONE OXIDIZES / DESTROYS BIOFILM

26. BIOFILM DISEASES
• Periodontal Disease
• Carious Disease
• Endodontic Disease
• Post Extraction
• Implant Issues

28. CurrentTherapies in Dentistry
• Caries (tooth decay)
• Infection of head and neck
• Periodontal Disease
• Root CanalTherapy
• Enhanced wound healing
(post-surgical)
• Soft tissue lesions

29. ISTHIS A LOCAL OR SYSTEMIC PROBLEM?

30. BOTH!!!

31. Pulp Exposure?

32. Deep Caries?
What’s under sealants?

33. ARRESTMENT OF CARIES

34. THE CASE FOR PIT AND FISSUE
SEALENTS WITH OXYGEN/OZONE

35. 60 second exposure 46µg/cc

36. 2 minutes post exposure

37. 46 micrograms/cc – 60 second exposure

38. OZONE

39. ENDODONTIC REALITY

40. FUMIGATION PROCEDURE WITH 40 – 50 µg/cc
EACH ROOT EXPOSURE TIME 40-60 SEC.

45. A “Dry Socket”?

46. Periodontal Problems?

47. CODE #162

48. TWO MONTHS OFTREATMENT
DR. JOSE DE CASTRO-COIMBRA

49. History of Ozone
CHRISTIAN FREDRICH SCHONBEIN
DISCOVERED OZONE IN 1840

50. • 1881- John Harvey Kellogg, MD, from Battle Creek, MI used ozone to treat diptheria.
• 1896- Nikola Tesla patented first O3 generator and sold them to doctors for medical use.
• 1898- Benedict Lust, MD, New York- Founder of Naturopathy, wrote about ozone therapy.

51. 1885 Florida Medical Association
Published: Ozone
• Written by Charles J. Kenworthy M.D.
• Practicing medicine in Jacksonville, Florida.
• Proved that ozone was in regular medical usage
in the United States before 1885.
• Therefore, it predates the 1906 Pure Food and
Drug Act and the FDA.
• Ozone for medical usage is grandfathered in the
United States, and held perfectly legal for use
without censure.

53. Ozone History in Dentistry
• 1932 - E.A. Fisch, a dentist uses ozonated water in dental
procedures and pioneered its use in medicine. (1932-1998 rise
and fall of antibiotics)
• 1998 - Edward Lynch publishes a series of articles
investigating the efficacy of ozone on dental infection.
• 1998 – Mollica, Harris first introduced to oxygen/ozone therapy
in dentistry to United States
• 2000 – Harris delivers first comprehensive oxygen/ozone
lecture at Capital University of Integrative Medicine.
• 2000 – Oxygen/Ozone becomes curriculum based at CUIM.
• 2000 – Oxygen/Ozone Therapy in Dentistry introduced in the
clinical setting at National Integrative Health Associates –
CUIM clinic
• 2002 – Mollica, Harris Oxygen-Ozone Therapy “Applications in
Biologic Dentistry”. Presented at first continuing education on
oxygen/ozone in dentistry

54. • 2004 – Edward Lynch – chief editor – Ozone: The
Revolution in Dentistry
• 2007 – Mollica, Harris Cyto-Immunologic Nutritional
Supportive Therapies for Oxygen/Ozone Therapy
• 2008 – International Academy of Oral Medicine and
Toxicology accepts Oxygen/Ozone Therapy in
Dentistry as scientifically valid.
• 2008 – IAOMT scientifically validates endodontic
therapy with oxygen/ozone (G. Cole)
• 2009 – IAOMT publishes Oxygen/Ozone in Dentistry
• 2009 – Academy of General Dentistry introduces
subject code 162 – Ozone Therapy

55. THE
SCIENCE
OF
OZONETHERAPY
CORONA
DISCHARGE

56. Properties of Ozone
InVitro (ExVivo) Powerful Oxidizer
–Kills bacteria, fungi and viruses at lower
concentrations than chlorine (1 molecule
of ozone = 3,000 to 10,000 molecules
of chlorine) AND kills 3500 times faster
than chlorine.
●Also oxidizes phenolics (poisonous compounds
of methanol and benzene), pesticides, detergents,
chemical manufacturing wastes and aromatic
compounds more rapidly and effectively than
chlorine, yet WITHOUT chlorine’s harmful
residues (Trihalomethanes, etc.).

57. Important Aspect to Consider
*AGAIN*
Oxygen/Ozone can accept
AND
Carry an electrical charge
Biofilm, Bacteria,Virus,
Fungi, Parasites,
Infection and
Inflammation all are
positively charged
Ozone has a
negatively
charged area

58. The In Vivo
Therapeutic Oxidative
BURST
0Z0NE
PLASMA
REACTIVE OXYGEN
SPECIES
ROS
(early phase)
LIPID OXIDATION
PRODUCTS
LOPs
(late phase)
O3
H2O2
O1

59. –Ozonated Water: Ozone gas is bubbled
through water, creating a solution that has a
shelf life of 10 hours at room temperature and
5 days in the refrigerator. Ozonated water is
used to treat:
• Wounds
• Burns
• Intestinal problems
• Slow healing skin infections
• Gynecological Infections
• Also used for colonics
• and enemas

60. – Ozonated Oil (primarily olive oil): Ozone is bubbled
through oil until it forms a salve/balm type consistency. This
is used to treat:
• Skin conditions, infections with bacteria, fungi and viruses.
• Hemorrhoids
• Vulvovaginitis
• Insect bites/bee stings
• Gingivitis
• Placed on capsules and ingested to treat ulcers, gastritis and
giradiasis

61. TMD Applications
FOLLOW “Standard of Care Procedures”
IF symptoms fail to resolve
THEN consider ozone treatment due to the probability of an
infection/inflammation in the joint(s) or chronic inflammation of
ligaments, tendons, muscles or ganglia

62. THE SCIENCE/RESEARCH OF OXYGEN/OZONE
ALLOWS FOR…….!!!
• NON-TOXIC SAFE AND EFFECTIVE APPLICATION
• TRUE BIOLOGIC AGENT
• MINIMALTO NO SIDE EFFECTS
• ECONOMICTHERAPY

63. IN A BIOLOGIC SENSE…….
• THE CREATION OF A THERAPEUTIC OXYGEN/OXIDATIVE
“BURST” WHICH INDUCES A MULTI-FACTORIAL BIOCHEMICAL/
PHYSIOLOGIC RESPONSE THERE BY REVERSING A STATE OF
ANAEROBIOSIS CONTAINED WITHIN THE AFFLICTED
TISSUES….MOLLICA
OTTO WARBURG

64. INTEGRATION OF OZONETHERAPY
INTO
DAILY BIOLOGIC DENTAL PRACTICE

65. NASAL INSUFFLATION

66. +O3 C10H18O3
TRIOZONIDE OF TRIOLEIN
NOT AN OXIDANT
OXIDATION OF OLIVE OIL
SO WHAT’STHE POINT?

67. – Inhalation of Ozonides: An ozone/oxygen mixture is bubbled
through olive oil and gaseous compounds are created that are a
species of triozonides of triolein (C10H18O3) that are safe to
inhale. These triozonides decompose at the aqueous level of the
lung alveolus. The thereby create oxidative peroxides that are
used to treat:
Allergies
Asthma
Other respiratory diseases
Sinus infections
Foundational procedure
THE CREATION OF AROMATIC TERPENES!

68. BIOFILM
An aggregate of microbes with a distinct
colonizing architecture.

69. LUNG ALVEOLUS
WITH BIOFILM FORMATION
DYSFUNCTIONAL CILIA DUE TO
MICROBIAL TOXINS FROM BIOFILM

70. EAR INSUFFLATION:
THE OXYGEN/OZONE BEING DELIVEREDTOTHETYMPANIC
MEMBRANE MUST BE MOISTENED PRIORTO CONTACT!
MOISTURE CAN BE DELIVERED VIA BUBBLING OXYGEN/OZONE THROUGH
THE OZONATED WATER SNAKE OR MOISTENED AT THE CUPS OF THE
STETHOSCOPE WITH WET GAUZE
(use a fan to blow the gas away from the face)

71. Ear Infections
(German Research)
17 patients received 15 sessions of rectal ozone plus ozonized oil
drops for chronic suppurative O.M. 16 tested negative at the end of
two cycles, and 14 improved their hearing loss. Mastoid x-ray
improvement noted in 13. Another study remarked on the
improvement noted using O2/O3 irrigations of theTM.
This is the preferred therapy for recurrent
otitis media as opposed to repeated and
prolonged use of antibiotics.

72. • Vestn Otorinolaringol. 2009;(3):34-5.
• [Treatment of patients with acute and chronic suppurative middle ear diseases using
ozone preparations].
• [Article in Russian]
• Sin'kov EV.
• Abstract
• Methods of clinical application of gaseous ozone and ozonated solutions are described
with special reference to the treatment of patients with acute and chronic suppurative
middle ear diseases. Results of otoscopy, microbiological and cytological studies confirm
therapeutic efficiency of the above techniques as accelerating recovery of patients with
this pathology. It is recommended to include ozonotherapy as an additional method in
the combined treatment of acute and chronic suppurative otitis media.
It is recommended to include ozonotherapy as an
additional method in the combined treatment of acute
and chronic suppurative otitis media.

73. Vestn Otorinolaringol. 1999;(2):48-9.
[Reasons for the application of medical ozone in the
treatment of chronic purulent mesotympanitis].
[Article in Russian]
Shakov VIu, Edeleva AN.
Abstract
Ozone-oxygen mixture (ozone concentration 600 microg/l) was used in
irrigations of the tympanic cavity in 52 patients with chronic purulent
mesotympanitis (CPM). The course of the treatment consisted of 5-7
procedures. 18 CPM controls received standard treatment without the
irrigations. The course of the ozone-oxygen irrigations produced good
results: ear discharge and tympanic mucosa inflammation stopped in 81%
of the irrigated patients, levels of myeloperoxidase which marks
inflammation reduced significantly, bactericidal effects of the mixture were
observed for all the detected pathogens, antibiotic sensitivity of the
bacteria rose. Fast antiinflammatory result of ozone therapy was due not
only to its bactericidal effect but also via its antihypoxic and
immunomodulating mechanisms
Fast antiinflammatory result of ozone therapy was due not
only to its bactericidal effect but also via its antihypoxic
and immunomodulating mechanisms

74. MULTI-TONSILULAR FORMS

75. TONSIL-FASCIA SPACE INFUSION
TECHNIQUE
• PRINCIPLE PART OF COMPREHENSIVE LYMPHATIC THERAPY
• PURPOSE: TO DRAIN AND DISENFECT THE TONSILS,
SURROUNDING FASCIA AND SOFT TISSUE(RETRO-
PHARYNGEAL SPACE)
• 6 TO 10µg/cc in syringe with 30g 1”needle
• Insert needle up to hub through tonsil.
• Slowing start infusion at the same time, slowly retract. This
will dispense oxygen/ozone throughout area.
• Infusion a total of 1 – 2cc per side
• Expectations: 24 – 72 hours drainage and possible outbreak
of pus-like lesions. This is good and will remediate within a
few days.

77. ONE INFUSION 2-3CC AT 6 - 10µG/CC

78. QUESTIONS??