Ozone is a naturally occurring molecule that protects us from radiation and binds to air pollutants. It is formed by sunlight and lightning and exists as a triatomic molecule. Ozone therapy uses ozone in gas, water, or oil forms to disinfect areas and stimulate healing in the body. When introduced into the body, ozone creates an oxidative burst that kills microorganisms but also improves blood flow, immune response, and healing. Ozone therapy can be used to treat dental issues like tooth decay, sensitivity, gum disease, and sockets after tooth extraction by disinfecting and accelerating the healing process.

2. Introduction
• Ozone is known to us as a molecule
present in the atmosphere which protects
us from radiation. It also binds to the
pollutants presents, hence used to
measure pollution levels.
• This is the job of ozone, to be nature’s
filter of “bad” particles.

3. WHAT IS OZONE ?
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Ozone (O3) is a triatomic molecule.
10-fold more soluble in water than oxygen.
Ozone is not a radical molecule
It is the third most potent oxidant.
It also has the capacity to stimulate blood
circulation and the immune response.

4. How is ozone produced ?
Ozone is naturally formed by the sun and
lightning in storms.

5. Ozone Generators

6. Forms of ozone used
• Ozone gas.
• Ozonated water.
• Ozonated olive oil.
These can be used singly or in combination

7. HOW DOES OZONE THERAPY WORK ?
• Ozone, when introduced into the living system,
creates what is called a "transient oxidative
burst". The infective microorganisms have no
natural defense against the reaction, and, as a
result, are overstressed and die. Thus, Ozone
disinfects the area treated.
• This "oxidative burst" also includes a multitude
of natural biochemical and physiological
reactions. These reactions include better
blood flow, enhanced immune response, and
a more rapid healing response.

8. CAN YOU BE ALLERGIC TO OZONE ?
• No, Ozone is just an energized form of
oxygen.

9. Safety from ozone
• Due to safety concerns, O3 gas was not
recommended for intra-oral use earlier.
• With the development of a foot pedal
activated dental handpiece with a suction
feature, O3 gas can now be used safely in
situations where diffusion is an important
factor.

10. Primary Pits & Fissures Caries
Proximal Caries Lesions
– As a general rule, in non-cavitated low speed
onset lesions, a non-invasive protocol should
be used first. In cavitated lesions, restoration is
a must.
– The tooth is treated with ozone can completely
stop all caries activity and in initial lesion even
reverse the damage and allow remineralization.

11. Hyper-Sensitive Teeth
– Non-carious hypersensitivity is due to many
contributing factors among which are
erosion, bite pressure, recessed gum, etc.
– After elimination of the cause(s), ozone
application might alleviate almost instantly the
pain felt by the patients from hypersensitive
teeth in most cases.
– Apply ozone for 40-60 sec on sensitive areas
then a remineralizing agent. Repeat if
necessary.

12. Root Canal Therapy
– Ozone is highly indicated in root canal therapy
due to its strong disinfection property and
absence of cytotoxicity at the recommended
concentration and form.
– The ozonated air can get to the narrowest
places in the canal and peri-apical region where
conventional liquids may not be able to reach,
disinfect the area and accelerate healing.
– Also it reduces the requirement of antibiotics as
the region is disinfected locally.
– Ozonated oil can be used as a root canal
dressing.

13. An adapted silicone cap and 25G needle
for ozone gas canal disinfection
In case a silicone cap is unpractical put the
suction tip close to the canal orifice

14. Periodontal Therapy
– Gingival and Periodontal diseases represent a
major concern both in dentistry and medicine.
– The majority of the contributing factors and
causes in the etiology of these diseases are
reduced or treated with ozone.
– Using ozonated water generously irrigate the
affected area during and after scaling, root
surface planning, and non-surgical pocket
curettage.
– Fill the pockets with ozonized olive oil using a
blunt 25G needle or any other appropriate tip.
Repeat once a week till seen fit.

16. Results of using ozonized olive oil three days after scaling.
Picture taken post scaling
Picture taken 3 days post scaling
showing accelerated healing

17. TMJ Peri-Articular Ozone Gas
Injection - Trigger Points
– The biostimulation and anti-inflammatory effects
of ozone help in the management of articulation
inflammatory diseases and muscular trigger
points.
– Chronic oxidative stress and elevated levels of
pro-inflammatory cytokines are commonly
found in these skeletal chronic inflammations
where ozone gas infiltration can contribute in
stimulating the anti-oxidant defense mechanism
and in balancing the immune response by
modulating the production of cytokines

18. OZONE GAS INJECTION INTO SUPERIOR JOINT SPACE.

19. Post-Extraction
– After final debridement of the socket, irrigate
with copious amounts of ozonated water and
then use gauze imbibed with ozonated water to
compress the extraction site. Before retiring the
patient, fill the socket with ozonized oil and
cover it with gauze, it improves healing and
prevents infection.

20. Alveolar socket after extraction
Ozonated water being used for irrigation

21. Peri-Implantitis
– Peri-implantitis is very bothering to both the
dentist and the patient.
– Ozone can play an important role and be used
as gas or in aqueous form.
– Ozonated water can also be used as an irrigant
during debridement and curettage.
– Also advise the patient to apply ozonized oil on
the affected area 3-4 times/day.

22. Results of the use of standard antiseptics and
ozonated oil
3 DAYS USE OF
0.12% CHLOEHEXIDINE
3 DAYS USE OF
OZONATED OLIVE OIL

23. Crowns & Bridges - Veneers
– A common occurrence we sometimes see
during the temporization phase in crowns,
inlays/onlays and veneers procedures is
hypersensitivity.
– Many factors might contribute in this event,
one of which is the presence of bacteria left
inside the opened dentinal tubules during
preparation. It is paramount to remove the
smear layer and disinfect the prepared
teeth before temporization and before
seating of the final restoration.

24. Use of ozonated air before temporization and cementing the
final prosthesis

25. Soft Tissue Lesions
– All kinds of infectious, inflammatory, traumatic,
burns, wounds, soft tissue lesions respond very
well to topical ozone treatment.
– The infected area is sealed with a PVC or
silicone cap and ozone gas applied for 1-2
minutes. In larger areas aqueous ozone is also
used. Repeat if necessary.
– In case of cyst fistula, insert a plastic needle
slowly in the passage of the fistula and inject 12 cc of ozone gas.
– In many instances, daily at home application of
ozonized olive oil is required.

26. EFFECT OF OZONE THERAPY ON SOFT TISSUE LESIONS
Herpetic lesion treated with 2
min application of ozone gas.
Picture taken 40 min post
ozone application.

27. Whitening with Ozone
– Due to the strong oxidation power of ozone,
researchers started looking on the ability of
ozone to whiten teeth.
– The results so far are promising.

28. Conclusion
• Modern dentistry is all about non/minimal
invasive dentistry. Ozone therapy in the
present stage is reducing caries activity,
improving periodontal health, and also
improving the healing time of oral lesions
and wounds.
Ozone therapy is bound to
revolutionize the way dentistry is
going to be practiced in the future.