Does Cryopreservation Stress Impact Genotype Integrity? A Case Study with Germplasm of Musa spp - Third International Symposium on Plant Cryopreservation, Asia Hotel, Bangkok, Thailand March 26-28, 2018
Establishment of an in vitro propagation and transformation system of Balani...PGS
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University
Abstract
Balanites aegyptiaca is a drought-tolerant but salt-sensitive tree species distributed in the tropical and arid lands in Africa and Asia; the seeds were used in biodiesel production. This study aimed to establish an in vitro propagation system of two B. aegyptiaca provenances from nodal and cotyledon explants. The explants were placed on Murashige and Skoog medium supplemented with different concentrations of 6-benzyladenine (BA) and thidiazuron (TDZ) for shoot induction. BA was significantly more effective in shoot induction from nodal explants. Three different Agrobacterium tumefaciens strains (EHA105, GV3101, and LBA4404) harboring the plasmid pCAMBIA2301 containing the nptII marker and gus reporter genes were used to establish a transformation system in B. aegyptiaca. Strain GV3101 resulted in the highest survival rates and highest number of explants positive in the GUS assay. This selected A. tumefaciens strain was used to introduce pBinAR containing the sequence encoding ERD10 (early responsive to dehydration 10) to produce salt-tolerant B. aegyptiaca plants.
Establishment of an in vitro propagation and transformation system of Balani...PGS
This lecture was a part of Plant Genetics Seminars - PGS 2017/2018 at Assiut University. These seminars organized by Dr. Ahmed Sallam, Department of Genetics, Faculty of Agriculture, Assiut University
Abstract
Balanites aegyptiaca is a drought-tolerant but salt-sensitive tree species distributed in the tropical and arid lands in Africa and Asia; the seeds were used in biodiesel production. This study aimed to establish an in vitro propagation system of two B. aegyptiaca provenances from nodal and cotyledon explants. The explants were placed on Murashige and Skoog medium supplemented with different concentrations of 6-benzyladenine (BA) and thidiazuron (TDZ) for shoot induction. BA was significantly more effective in shoot induction from nodal explants. Three different Agrobacterium tumefaciens strains (EHA105, GV3101, and LBA4404) harboring the plasmid pCAMBIA2301 containing the nptII marker and gus reporter genes were used to establish a transformation system in B. aegyptiaca. Strain GV3101 resulted in the highest survival rates and highest number of explants positive in the GUS assay. This selected A. tumefaciens strain was used to introduce pBinAR containing the sequence encoding ERD10 (early responsive to dehydration 10) to produce salt-tolerant B. aegyptiaca plants.
Effect of different treatments on dormancy breaking of wild oat (Avenafatua) ...Innspub Net
To study the effect of different treatments on dormancy breaking of Avena fatua eight experiments based on randomized complete design (RCD) with five replications were conducted in the Arsanjan Islamic Azad Univetrsity, Fars province. Dormancy breaking treatments included the application of different concentrations of gibberellin, sulfuric acid, warm water, stratification(chilling), scarification, different temperatures, rinsing, and the use of ethanol. The results showed thatthe highest percent of germination was found in the stratification period of 2 to 3 weeks at 2-5 ° C in which germination rate was over 70%. Gibberellin application with a concentration of 600 ppm led to wild oat braking dormancy with the the maximum seed germination of 36%.The wild oat seeds exposure to sulfuric acid also led to dormancy breaking where the highest germination of 36 % was obtained by a 8-hour seed expoure. The results of concentration of sulfuric acid showed that the highest seed germination was 42% in treatment via concentration sulfuric acid 15%. In addition, our findings indicated that rinsing, warm water application, constant temperatures were not effective treatment forwild oat
dormancy breaking.
Identification of defense proteins in pearl millet seeds effective against Ma...ICRISAT
Pearl millet leaf blast is caused by Magnaporthe grisea (Anamorph, Pyricularia grisea) has been recently emerged as devastating disease with economic significance in India. It is well-known that host plant resistance is the most economical strategy to effectively manage this disease; hence, identification of resistance sources for blast disease is important to incorporate resistance genes into elite breeding lines. On the other hand, fungal cell wall is a multi-layered, in which chitin and glucan are the major polysaccharide constituents (Figure 1). In this view, chitinases and glucanases gain significant attention as antifungal enzymes. These were produced as pathogenesis related (PR) hydrolses in plants with constitutive expression in seeds, leaves, flowers, tubers and induced upon pathogen invasion. They exert their defensive role by decomposing the fungal cell wall polysaccharides chitin and glucan into respective monomers as N-Acetyl D-glucosamine and D-glucose residues (Prasannath, 2017). Whereas, protease inhibitors (PIs) are known to participate in defensive role by inhibiting the extracellular protease activity secretes from actively growing fungal mycelia as well as cysteine proteases involved in the chitin synthase activity (Joshi et al., 1998). Hence, the present study is focused on the screening of chitinases, glucanases and cysteine protease inhibitors in ten pearl millet seed proteins with differential disease resistance and evaluation of their anti fungal efficacy against growth of P. grisea (Pg 45), prevalent isolate in Hyderabad, Telangana region.
iDiffIR: Identifying differential intron retention from RNA-seqAraport
iDiffIR is a method for identifying differential intron retention from RNA-seq. For more information, please visit http://combi.cs.colostate.edu/idiffir/
Comparative analysis of some biochemical responses of winter and spring wheat...Innspub Net
To compare changes of biochemical indices between spring (Kavir) and winter (Azar2) cultivars of wheat (Triticum aestivum L.) under low temperature, 14 days old wheat seedlings were exposed to cold. The seedlings were transferred into growth chamber for 9 days at 5/3 °C (day/night) as cold treatment, or at 20/18 °C as control. Proline content, total protein accumulation, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) enzymes, were assayed in the leaf extracts of control and cold treated plants. The results showed that cold led to an accumulation of proline and an increase in protein level, especially in winter cultivar. Rapid increases in proline and protein accumulations were observed during early stages of cold stress. SOD activity displayed no significant differences between the two cultivars during the first 3 days after cold stress, while in Azar 2, the level of SOD activity was gradually increased after 3 days of cold stress. The POD and CAT activity were higher in plants grown at cold stress than in the controls; however, their rate was different in winter and spring wheat cultivars. In general, Azar2 showed relatively higher POD and CAT activity compared to Kavir. Regarding antioxidant enzymes activities, cultivars respond differently under cold stress. Articles source: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Short duration pigeonpea hybrids to bridge decades old yield gapICRISAT
Low yield in pigeonpea has always been a matter of concern for all the stakeholders over more than last five decades. But, development of CMS based hybrid technology in pigeonpea, has proved the potential to bridge this yield gap. Several medium duration hybrids have been released in states of India, yielding 25-40% higher yield than local varieties, but these hybrids are region specific due to its photothermo sensitive nature. Hence, need was felt to explore other possibilities to increase pigeonpea production and productivity. Recently developed photo-thermo incentive short duration varieties have helped to identify new production niches like wheat and rice cropping system in non-traditional states of India. But over years, productivity of these short duration varieties has also become stagnant. Considering this, hybrid breeding program was initiated at ICRISAT to develop location specific short duration hybrids. Nine short duration experimental hybrids along with two checks were evaluated for its yield and adaptability at ICRISAT, Patancheru and RARI, Durgapura Rajasthan for three years (2013-15) using GGE biplot analysis. Among the six environments evaluated, Patancheru (Pat-15) was identified as suitable location in discriminating short duration pigeonpea hybrids and can be considered as ideal testing location. ICPH 2364 followed by ICPH 3310 were earliest to mature while ICPH 2429 and ICPH 2433 were found to be high yielders and stable.
Effect of different treatments on dormancy breaking of wild oat (Avenafatua) ...Innspub Net
To study the effect of different treatments on dormancy breaking of Avena fatua eight experiments based on randomized complete design (RCD) with five replications were conducted in the Arsanjan Islamic Azad Univetrsity, Fars province. Dormancy breaking treatments included the application of different concentrations of gibberellin, sulfuric acid, warm water, stratification(chilling), scarification, different temperatures, rinsing, and the use of ethanol. The results showed thatthe highest percent of germination was found in the stratification period of 2 to 3 weeks at 2-5 ° C in which germination rate was over 70%. Gibberellin application with a concentration of 600 ppm led to wild oat braking dormancy with the the maximum seed germination of 36%.The wild oat seeds exposure to sulfuric acid also led to dormancy breaking where the highest germination of 36 % was obtained by a 8-hour seed expoure. The results of concentration of sulfuric acid showed that the highest seed germination was 42% in treatment via concentration sulfuric acid 15%. In addition, our findings indicated that rinsing, warm water application, constant temperatures were not effective treatment forwild oat
dormancy breaking.
Identification of defense proteins in pearl millet seeds effective against Ma...ICRISAT
Pearl millet leaf blast is caused by Magnaporthe grisea (Anamorph, Pyricularia grisea) has been recently emerged as devastating disease with economic significance in India. It is well-known that host plant resistance is the most economical strategy to effectively manage this disease; hence, identification of resistance sources for blast disease is important to incorporate resistance genes into elite breeding lines. On the other hand, fungal cell wall is a multi-layered, in which chitin and glucan are the major polysaccharide constituents (Figure 1). In this view, chitinases and glucanases gain significant attention as antifungal enzymes. These were produced as pathogenesis related (PR) hydrolses in plants with constitutive expression in seeds, leaves, flowers, tubers and induced upon pathogen invasion. They exert their defensive role by decomposing the fungal cell wall polysaccharides chitin and glucan into respective monomers as N-Acetyl D-glucosamine and D-glucose residues (Prasannath, 2017). Whereas, protease inhibitors (PIs) are known to participate in defensive role by inhibiting the extracellular protease activity secretes from actively growing fungal mycelia as well as cysteine proteases involved in the chitin synthase activity (Joshi et al., 1998). Hence, the present study is focused on the screening of chitinases, glucanases and cysteine protease inhibitors in ten pearl millet seed proteins with differential disease resistance and evaluation of their anti fungal efficacy against growth of P. grisea (Pg 45), prevalent isolate in Hyderabad, Telangana region.
iDiffIR: Identifying differential intron retention from RNA-seqAraport
iDiffIR is a method for identifying differential intron retention from RNA-seq. For more information, please visit http://combi.cs.colostate.edu/idiffir/
Comparative analysis of some biochemical responses of winter and spring wheat...Innspub Net
To compare changes of biochemical indices between spring (Kavir) and winter (Azar2) cultivars of wheat (Triticum aestivum L.) under low temperature, 14 days old wheat seedlings were exposed to cold. The seedlings were transferred into growth chamber for 9 days at 5/3 °C (day/night) as cold treatment, or at 20/18 °C as control. Proline content, total protein accumulation, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) enzymes, were assayed in the leaf extracts of control and cold treated plants. The results showed that cold led to an accumulation of proline and an increase in protein level, especially in winter cultivar. Rapid increases in proline and protein accumulations were observed during early stages of cold stress. SOD activity displayed no significant differences between the two cultivars during the first 3 days after cold stress, while in Azar 2, the level of SOD activity was gradually increased after 3 days of cold stress. The POD and CAT activity were higher in plants grown at cold stress than in the controls; however, their rate was different in winter and spring wheat cultivars. In general, Azar2 showed relatively higher POD and CAT activity compared to Kavir. Regarding antioxidant enzymes activities, cultivars respond differently under cold stress. Articles source: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Short duration pigeonpea hybrids to bridge decades old yield gapICRISAT
Low yield in pigeonpea has always been a matter of concern for all the stakeholders over more than last five decades. But, development of CMS based hybrid technology in pigeonpea, has proved the potential to bridge this yield gap. Several medium duration hybrids have been released in states of India, yielding 25-40% higher yield than local varieties, but these hybrids are region specific due to its photothermo sensitive nature. Hence, need was felt to explore other possibilities to increase pigeonpea production and productivity. Recently developed photo-thermo incentive short duration varieties have helped to identify new production niches like wheat and rice cropping system in non-traditional states of India. But over years, productivity of these short duration varieties has also become stagnant. Considering this, hybrid breeding program was initiated at ICRISAT to develop location specific short duration hybrids. Nine short duration experimental hybrids along with two checks were evaluated for its yield and adaptability at ICRISAT, Patancheru and RARI, Durgapura Rajasthan for three years (2013-15) using GGE biplot analysis. Among the six environments evaluated, Patancheru (Pat-15) was identified as suitable location in discriminating short duration pigeonpea hybrids and can be considered as ideal testing location. ICPH 2364 followed by ICPH 3310 were earliest to mature while ICPH 2429 and ICPH 2433 were found to be high yielders and stable.
Effects of 60Co gamma radiation doses on seed germination of Jatropha curcas ...Innspub Net
By: Baudouin K. Nyembo, Alexandre N. Mbaya, Calvin C. Ilunga, Jean-Louis N. Muambi, Luc L. Tshilenge
Key Words: Jatropha curcas, Gamma irradiation, Seed germination, LD50.
Int. J. Agron. Agri. Res. 13(5), 46-52, November 2018.
This study aimed to assess the effects of different gamma radiation doses from Cobalt -60 isotopic source on seed germination and early growth parameters of Jatropha curcas L. Healthy and dry seeds were subjected to three doses of gamma rays (100, 200 and 300 Gy). The experiment was conducted using randomized complete block design, with three replicates. The significantly maximum germination percentage (89.85 %), seedling survival (92.3 %), seedling collar diameter (0.892 cm), plant height (17.30 cm), number of leaves (7) were observed at 30 days after germination. The results revealed that seed germination percentages and seedling shoot length decreased with increasing dose of gamma-rays. Higher gamma-ray dose (300 Gy) in particular had a pronounced effect on these germination parameters than others, probably because high-dose inhibited cell division due to free radicals and DNA system damage. The LD50 for seeds germination rates was obtained at 254 Gy. These results implied that germination traits of Jatropha curcas seeds were sensitive to increase in gamma-ray.
Detection of Genetic variation in tissue culture clones of date palm using IS...IJSRD
Date palm is a plant having high nutritional value and long life (yielding up to 100 years). Phoenix dactylifera requires 2-5 males for pollination of 100 females’ plant depending up on genetic and environment factors. Therefore paternity variation expected to very low according to PCR based techniques, Even though we have tried to find out genetic variation among tissue culture cloned plant. Tissue culture technique can be used for genetic improvement of date palm. The main purpose of this study was to evaluate the genetic variation in the tissue culture clones of date palm by using ISSR primers among mother and it’s two clones. The plant DNA was extracted and subjected to detection of genetic variation in two groups of date palm using ISSR primers. In this study ISSR primers produced monomorphic bands within group-1 and group-2. Genetic variation in tissue culture clones of date palm was not detecte by UBC primer series.
Induced Mutagenesis of Flowering, Phenology and Yield in M1 Generation of Bam...ijtsrd
A highly cherished Bambara groundnut variety, ‘Caro’ was treated with different concentrations of ethyl methane sulphonate EMS for different durations to raise an M1 generation. In the M1 generation, the mutagenic effect of EMS on seed germination, growth and seed yield at different doses 0.01 , 0.1 , 0.25 and 0.5 and for different durations 6 hours, 12 hours and 24 hours were studied. Characters like days to first flowering, days to 50 flowering, number of flowers per plant, number of leaves, number of nodes per plant, number of stems per plant, plant spread, plant height, number of branches per plant, pod length, pod width, number of pods per plant, number of seeds per plant, seed length, seed width and seed yield were measured. Data collected were subjected to analysis of variance ANOVA and means were separated using least significant difference LSD . The result showed that there was significant effect p 0.05 of ethyl methane sulphonate concentrations in all the parameters except on number of days to maturity. The concentration, 0.1 and soaking duration of 6 hours was more promising in creating variability. Apparently, could Ethyl methane sulphonate significantly improved the performance and create genetic variability of Bambara groundnut and therefore can be employed in breeding programme for Bambara groundnut. Grace O. Chimdi | Michael I. Uguru | Peter. E. Ogbonna | Nwakuche C. Onwubiko "Induced Mutagenesis of Flowering, Phenology and Yield in M1 Generation of Bambara Groundnut (Vigna Subterrnea (L.) Verdc)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-2 , February 2022, URL: https://www.ijtsrd.com/papers/ijtsrd49327.pdf Paper URL: https://www.ijtsrd.com/other-scientific-research-area/other/49327/induced-mutagenesis-of-flowering-phenology-and-yield-in-m1-generation-of-bambara-groundnut-vigna-subterrnea-l-verdc/grace-o-chimdi
Research Program Genetic Gains (RPGG) Review Meeting 2021: From Discovery to ...ICRISAT
Chickpea (Cicer arietinum) is the second most widely grown legume crop after soybean, accounting for a substantial proportion of human dietary nitrogen intake and playing a crucial role in food security in developing countries. We report the∼ 738-Mb draft whole genome shotgun sequence of CDC Frontier, a kabuli chickpea variety, which contains an estimated 28,269 genes. Resequencing and analysis of 90 cultivated and wild genotypes from ten countries identifies targets of both breeding-associated genetic sweeps and breeding-associated balancing selection. Candidate genes for disease resistance and agronomic traits are highlighted, including traits that distinguish the two main market classes of cultivated chickpea—desi and kabuli.
Phylotype Analysis of Ralstonia Solanacearum Causing Bacterial wilt in Eggpla...ijtsrd
Eggplant is prone to attack by several pests including bacteria, fungi, nematodes and insects. In this study, we have analyzed phylotype of bacterial wilt Ralstonia solanacearum infection in eggplant plants collected from Bhubaneswar Orissa in India. Bacterial wilt symptomatic five plant samples were collected from brinjal field in Bhubaneswar in 2016. The samples were macerated in sterile distilled water and grown on Kelman's triphenyltetrazolium chloride TZC agar media. Total genomic DNA of the bacterium were extracted and subjected to PCR amplification using the R. solanacearum specific universal primer pair 759 760. An expected single 280 bp fragment amplified in all the samples confirmed the identity of these as Ralstonia. To reconfirmed isolate of bacterium, the amplicon was sequenced in sequencer. In NCBI blast, the nucleotide sequence was 100 similar with Ralstonia solanacearum strain RS lpxC DOB 1 AB910593 and the sequence was submitted in NCBI database under Acc. No. KY393266. To determined phylotype of strain used specific multiplex PCR with phylotype specific primers Nmult 21F1 2, Nmult 22InF, Nmult 23AF, Nmult 22RR revealed that all the five infected samples belonged to phylotype I as a 144 bp amplicon were observed in agarose gel. On the basis of above finding concluded that the bacterial wilt infected eggplant collected from Bhubaneswar was Ralostonia solanacearum, Phylotype I. Rakesh Kumar | Ramachandran, E. | Koteshwar Yadav "Phylotype Analysis of Ralstonia Solanacearum Causing Bacterial wilt in Eggplants in Orissa in India" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21580.pdf
Dioscorea rotundata is a staple food crop for millions of people in the tropical and subtropical regions. In vitro germplasm conservation is a very useful tool in yam improvement strategies but very little is known about the genetic integrity and stability of in-vitro conserved yam plants. In this study, 42 accessions from in vitro and field populations were genotyped using 11 microsatellite markers and 23 morphological descriptors to assess variability within and between accessions. Out of the 23 morphological variables used, 13 were identified as most discriminate and were used to cluster the accessions into 4 clusters using the unweighted pair group arithmetic mean average (UPGMA). Accession maintained in field as well as in in-vitro showed high genetic similarity (R2 = 0.91, p-value: 1e-04). Out of the 42 accessions analyzed, nine accessions maintained in the field and in-vitro displayed different genetic profiles. This study provided basic information on the possible somaclonal variation of yam accessions maintained through in-vitro. Further study with advanced tools such as next-generation sequencing is required to elucidate the nature of the observed variation within clones.
S M Masiul Azam, Md Shahidul Islam, Parvin Shahanaz, Md Shafiqur Rahman and Sarder Md Shahriar Alam. “Molecular Characterization of Brassica Cultivars through RAPD Markers” United International Journal for Research & Technology (UIJRT) 1.3 (2019): 41-45.
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Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
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Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
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What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
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Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
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heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Does Cryopreservation Stress Impact Genotype Integrity? A Case Study with Germplasm of Musa spp
1. Anuradha Agrawal, R. Sanayaima, R. Goswami, J. Goenka, D.P. Meena, Shivani, R.
Singh, S. Uma1 and Rishi Kumar Tyagi2
National Bureau of Plant Genetic Resources (ICAR-NBPGR), New Delhi – 110 012, INDIA
1National Research Centre for Banana (ICAR-NRCB), Tiruchirapalli, Tamil Nadu, INDIA
2 Coordinator, Asia-Pacific Consortium on Agricultural Biotechnology and Bioresources
(APCoAB), Asia-Pacific Association of Agricultural Research Institutions (APAARI)
Bangkok 10100, THAILAND
Does Cryopreservation Stress Impact Genotype Integrity?
A Case Study with Germplasm of Musa spp.
ThirdInternationalSymposiumonPlantCryopreservation
AsiaHotel,Bangkok,Thailand,March26-28,2018
3. • Immense economic importance and diversity (>300 cultivars,
several endemic wild species like M. cheesmannii, M. sikkimensis,
M. nagensium and M. andamanica)
• Loss of habitat and local landraces
• Climate change and other unknown biotic and abiotic stresses
Urgency for Musa Conservation in India
Musa arunachalensis: a new species of section
Rhodochlamys from Arunachal Pradesh, India
Ref: Sreejith et al. Phytotaxa 134 (1): 49–54 (2013)
Arunachal Pradesh, India
wild bananas
5. Musa Conservation in Complimentary Mode
Field Gene Bank of NRCB, Trichy, India
(1993)
Cryobank of NBPGR, New Delhi,
India (2004)
•Absence of seeds in most of the triploid
Musa cultivars
•Predominantly vegetatively propogated
In Vitro Genebank of NBPGR, New
Delhi, India (1986)
6. Comparison of cryopreservation protocols in banana
cv Robusta (AAA)
Ref: Agrawal A., Panis B., Swennen R. (2004) CryoLetters 25: 101-110
Simple
freezing of
PM
Vitrification
of PM
Droplet
Vitrification
of PM
Droplet
Vitrification
of IM
0
20
40
60
80
100
120
C LN C LN C LN C LN
SF V DV DV-IM
Shootregeneration(%)
% Callus
% Shoot
a
d
b
bc
bc
bc
b
b
8. Studies on regenerated plants from cryopreserved
meristems of Musa
• Survival
• Regrowth of shoot
• Recovery of plantlets
• Hardening and field transfer
• Morphological evaluation of regenerants
• SSR analysis of regenerants
10. 0
20
40
60
80
100
120
C LN C LN C LN C LN C LN
30 min. 60 min. 90 min. 120 min. 150 min.
Survival(%)
PVS2
Callus
Shoota
e
a
de
a
cd
b
cd
bc
e
Pidi Monthan (ABB)
0
20
40
60
80
100
120
C LN C LN C LN C LN C LN
30 min. 60 min. 90 min. 120 min. 150 min.
PVS2
Survival(%)
Callus
Shoot
cd
a
a a a
cd
cd bc
ab
d
Sommrani Monthan (ABB)
0
20
40
60
80
100
120
C LN C LN C LN C LN C LN
30 min. 60 min. 90 min. 120 min. 150 min.
PVS2
Survival(%)
Callus
Shoot
a
e
ab
de
a
cd
a
cd
bc
c
Kallu Monthan (ABB)
Agrawal A. et al. (2008) Current Science 94:1125-1128
Vitrification Protocol in Indian Cooking Bananas (Monthan subgroup)
11. Descriptors Recorded (IPGRI 1996)
1 Leaf habit,
2 Pseudostem color
3 Pseudostem appearance
4
Predominant underlying color of the
pseudostem
5
Pigmentation of the underlying
pseudostem
6 Wax on the leaf sheaths
7 Appearance of the leaf upper surface
8 Color of leaf upper surface
9 Color of leaf lower surface
10 Wax on leaves
11 Insertion point of leaf blades on petiole
12 Shape of leaf blade base
13 Color of midrib dorsal surface
14 Peduncle color
15 Peduncle hairiness
Contd.
16 Male bud type
17 Male bud shape
18 Male bud size
19 Bract
20 Bract apex shape
21 Bract imbricate
22 Color of bract external face
23 Color of the bract internal face
24 Color on the bract apex
25 Male flower behavior
26 Compound tepal basic color
27 Compound tepal pigmentation
28 Lobe color of compound tepal
29 Lobe development of compound
tepal
30 Free tepal color
31 Free tepal shape
FlowerVegetative
12. 32 Free tepal appearance
33 Free tepal apex appearance
34 Free tepal apex shape
35 Anther exertion
36 Filament color
37 Style basic color
38 Pigmentation on style
39 Style exertion
40 Style shape
41 Stigma color
42 Ovary shape
43 Ovary basic color
44 Ovary pigmentation
45 Dormant color of the male
flower
46 Bunch position
47 Bunch shape
48 Bunch appearance
49 Rachis type
50 Rachis position
51 Fruit shape
52 Transverse section of fruit
53 Fruit apex
54 Remains of flower relics at
fruit apex
55 Pedicel surface
56 Fusion of pedicels
57 Immature fruit peel color
58 Mature fruit peel color
59 Adherence of the fruit peel
60 Cracks in fruit peel
61 Pulp color before maturity
62 Pulp color at maturity
63 Fruit fall from hands
64 Flesh texture
Flower Fruit
13. Cryopreserved banana cv Pidi Monthan (ABB) with fruiting bunch, at
NBPGR, New Delhi (left) and NRCB, Trichy (right)
14. NRC on Banana, TrichyNBPGR, New Delhi
Cryopreserved plants with fruiting bunch, at NBPGR, New Delhi (left) and NRCB,
Trichy (right)
Musa ABB cv ‘Sommrani Monthan’
15. Parameters
TC
Control
Cryo
Control
LN
treated
Sucker
Control
MSE
C.D @
1%
CV %
Pseudostem Height 275.00 281.75 282.00 282.50 8.45 6.68 1.04
Pseudostem Girth 62.00 61.50 60.75 62.00 5.45 NS 3.79
No. of leaves at Shooting 13.75 15.00 15.50 14.75 0.33 1.32 3.89
Petiole length 62.75 63.00 63.75 61.25 1.12 2.43 1.69
Leaf area in sq. m 14.65 15.97 16.93 15.98 345.32 1.35 3.70
Time taken for shooting 362.25 366.50 363.50 366.00 67.90 NS 2.26
Time taken for bunch
maturation
115.00 115.00 113.75 113.00 22.78 NS 4.18
Duration 477.25 481.50 477.25 479.00 42.00 NS 1.35
Bunch weight (in kg) 15.62 16.25 15.62 15.75 0.26 NS 3.23
No. of hands/ bunch 8.75 8.50 8.50 8.75 0.36 NS 6.96
No. of fruits / hand 13.25 13.75 13.75 13.25 0.28 NS 3.92
Total no. of fruits 116.00 116.75 116.75 116.00 81.25 NS 7.75
Growth and Yield Data of Cryopreserved Musa accession (ABB)
Agrawal A. et al. (2011) Acta Horticulturae 908: 129-138
16. Sommrani Monthan (ABB)
Fruit from Non-
Cryopreserved
Plant (Ash
coated fruits)
Fruit from
Cryopreserved
Plant
(Green fruits)
Agrawal A. et al. (2011) Acta Horticulturae 908: 129-138
17. What caused the reversion?
Stress from tissue culture or cryopreservation
(PVS2, Freeze stress, Thawing injury)?
18. Sl. No. Primer name Primer sequence
1. AGMI 33 AGTTTCACCGATTGGTTCAT
AGMI 34 TAACAAGGACTAATCATGGGT
2. AGMI 35 TGACCCACGAGAAAAGAAGC
AGMI 36 CTCCTCCATAGCCTGACTGC
3. AGMI 67 ATACCTTCTCCCGTTCTTCTTC
AGMI 68 TGGAAACCCAATCATTGATC
4. AGMI 93 AACAACTAGGATGGTAATGTGTGGAA
AGMI 94 GATCTGAGGATGGTTCTGTTGGAGTG
5. AGMI 95 ACTTATTCCCCCGCACTCAA
AGMI 96 ACTCTCGCCCATCTTCATCC
6. AGMI 103 ACAGAATCGCTAACCCTAATCCTCA
AGMI 104 CCCTTTGCGTGCCCCTAA
7. AGMI 129 GGAGGCCCAACATAGGAAGAGGAAT
AGMI 130 CATAAACGACAGTAGAAATAGCAAC
8. Mb SSR 1-146 F CCGTTGGATTTCTCCCCCACA
Mb SSR 1-146 R GAAGAACTGGGCTTACCCAGGA
9. Mb SSR 1-149 F CCGAAACGAAGGTTACAACAA
SSR primers used for Sommrani Monthan analysis
20. Green mutant
Ash colour fruit
•Our initial study showed
that 100% of the in vitro-
conserved and 93.3% of the
cryopreserved Musa
germplasm was genetically
stable.
•But the single plant
reversion prompted further
studies to be undertaken in
other accessions.
21. Cryopreservation of Sucker Meristems (SM)
by Droplet Freezing Technique in Musa ABB cv. Karpura Chakkarakeli
22. 0
20
40
60
80
100
120
C LN C LN C LN
SF VIT DF
Survival(%)
Callus
Shoot
b
ba ab
a
a
0
20
40
60
80
100
120
C LN C LN C LN
PM IVM SM
Survival(%)
Callus
Shoot
a
a
a
a
a
a
2 months8 months14 months
Use of sucker meristems
(SM) for cryopreservation
of banana was successful
The duration of
cryopreserving plants from
SM was only 2 months and
this cuts down the cost of in
vitro maintenance of
cultures
Amongst the three
cryopreservation
methods, droplet
vitrification method
gave the highest
number of shoot
during regeneration
Agrawal et al., 2014. In Vitro Cellular and Developmental Biology – Plant 50:345–356
23. Tissue culture control Fruiting of cryopreserved plant
derived from proliferating meristems
Fruiting of cryopreserved plant derived
from in vitro single shoot meristems
Fruiting of cryopreserved plant
derived from sucker meristems
Fruiting of cryopreserved plants in Karpura Chakkrakeli (ABB)
24. Primer Sequence
Melting
temperatur
eTM (ºC)
Annealing
temperature
TA (ºC)
No. of
scorable
bands
BN-01F AAG AAG GCA CGA GGG TAG 56
BN-01R CGA ACC AAG TGA AAT AGC G 56
BN-02F GGA AAA CGC GAA TGT GTG 54
57.4 1
BN-02R AGC CAT ATA CCG AGC ACT TG 60
BN-03F CGT CAC AGA AGA AAG CAC TTG 62
50.9 2
BN-03R CCT CTC CAT CGT CAT CAA TC 60
BN-04F GAG CCC ATT AAG CTG AAC A 54
BN-04R CCG ACA GTC AAC ATA CAA TAC A 62
BN-05F TGA GGC GGG GAA TCG GTA 58
BN-05R GGC GGG AGA CAG ATG GAG TT 64
BN-06F AAG AAG GCA CGA GGG TAG 56
60 2
BN-06R CGA ACC AAG TGA AAT AGC G 56
BN-07F GGA AAA CGC GAA TGT GTG 54
50 1
BN-07R AGC CAT ATA CCG AGC ACT TG 60
BN-08F ATG TCG CTT CGG ACC AGA 56
58.5 3
BN-08R GCA GGA CGA AGA ACT TAC C 58
BN-09F ATG ATC ATG AGA GGA ATA TCT 56
50.3
1
BN-09R TCG CTC TAA TCG GAT TAT CTC 60
BN-10F GGT TGG AAC GGA GGT ATA CTA A 64 50.9 2
BN-10R TCC AAG CTT ATC GAT CTA CG 58
Genetic stability
Analysis using SSR
Primers
25. Primer Sequence TM (ºC) TA (ºC)
No. of scorable
bands
BN-11F TGT CGA AGC ATC CTA CAT C 56
56 1
BN-11R CTT GGA AAC ATG AGA AAC ATA C 60
BN-12F TTG AAG TGA ATC CCA AGT TTG 58
50.9 2
BN-12R AAA ACA CAT GTC CCC ATC TC 58
BN-13F TGC TCT TCC ACA TCT CAA GAA C 64
59.8 1
BN-13R GAT TGC ACG GAG ATT CAA CA 58
BN-14F GGT GCT CTT CGG AGG A 52
59.8
BN-14R CGC TTT ATA TCC ATT CCC A 54
BN-15F GAG CCC ATT AAG CTG AAC A 56
59 1
BN-15R CCG ACA GTC AAC ATA CAA TAC A 62
BN-16F GAT GAT GGT GAG AGG CTG ATG A 66
60
BN-16R GGT CGG TAT GGG AAG CAC C 62
BN-17F TTT GCC TGG TTG GGC TGA 56
50 3
BN-17R CCC CCC TTT CCT CTT TTG C 60
BN-18F GAG CCC ATT AAG CTG AAC A 54
60 1
BN-18R CCG ACA GTC AAC ATA CAA TAC A 62
BN-19F CTC CTT TGT GAG CTC GGC ATA T 66
50.3 1
BN-19R AGG GTC CAA GAA ACT CCT CCA A 66
BN-20F GAA GCA TCC AAT GGA CCT A 56
60 1
BN-20R GCG AAC TCA CAA TAG CGA 54
SSRprimers
used
26. Primer Sequence TM (ºC) TA (ºC)
No. of scorable
bands
BN-21F CCC GTC CCA TTT CTC A 50
59.8 1
BN-21R TTC GTT GTT CAT GGAATC A 52
BN-22F GGT GGA TGG CTG GGT A 52
59.8 1
BN-22R GGA TCC AAG CTT ATC GAG TT 58
BN-23F GGT GCT CTT CGG AGG A 52
58.5 1
BN-23R CGT TTA TAT CCA TTC CCA 50
BN-24F GCT TGT CTC TCA CCC ACT C 60
50 1
BN-24R ACC GAC TCC CCAATA GG 54
BN-25F CTG GTC CTT TTC AGT TCA CTC 62
50.3 3
BN-25R TAG GCA GCT CCC AAT CA 52
BN-26F CTA GGC TTC CTG CTG CTC 58
60
BN-26R TGA GCG AAT TTG ATC AGAAC 56
BN-27F GCA CGAAGA GGC ATC AC 54
54.3 2
BN-27R GGC CAAATT TGA TGG ACT 68
SSRprimers
used
27. SSR Profile of Cryopreserved Plants
BNR 13
BNR 17
BNR 18
In all the
cryopreserved plants
regardless of the
method used, plants
show 92% similarity,
based on 24 SSR
primer pairs
29. Primer Sequence
Melting temperatureTM
(ºC)
Annealing
temperature
TA (ºC)
Ma-1-132 GGA AAA CGC GAA TGT GTG 54
57.4
AGC CAT ATA CCG AGC ACT TG 60
Ma-SSR-18 CGT CAC AGA AGA AAG CAC TTG 62
50.9
CCT CTC CAT CGT CAT CAA TC 60
Ma-SSR-07 AAG AAG GCA CGA GGG TAG 56
60
CGA ACC AAG TGA AAT AGC G 56
Ma-SSR-07 GGA AAA CGC GAA TGT GTG 54
50
AGC CAT ATA CCG AGC ACT TG 60
Ma-1-136 ATG TCG CTT CGG ACC AGA 56
58.5
GCA GGA CGA AGA ACT TAC C 58
Ma-1-127 ATG ATC ATG AGA GGA ATA TCT 56
50.3
TCG CTC TAA TCG GAT TAT CTC 60
Ma-1-5 GGT TGG AAC GGA GGT ATA CTA A 64 50.9
TCC AAG CTT ATC GAT CTA CG 58
SSR primers used (21)
Contd…
30. Primer Sequence TM (ºC) TA (ºC)
Ma-1-230 TGT CGA AGC ATC CTA CAT C 56 56
CTT GGA AAC ATG AGA AAC ATA C 60
Ma-1-27 TTG AAG TGA ATC CCA AGT TTG 58 50.9
AAA ACA CAT GTC CCC ATC TC 58
Ma-1-18 TGC TCT TCC ACA TCT CAA GAA C 64 59.8
GAT TGC ACG GAG ATT CAA CA 58
Ma-2-2 GAG CCC ATT AAG CTG AAC A 56 59
CCG ACA GTC AAC ATA CAA TAC A 62
Ma-1-16 TTT GCC TGG TTG GGC TGA 56 50
CCC CCC TTT CCT CTT TTG C 60
Ma-1-24 GAG CCC ATT AAG CTG AAC A 54 60
CCG ACA GTC AAC ATA CAA TAC A 62
Ma-2-4 CTC CTT TGT GAG CTC GGC ATA T 66 50.3
AGG GTC CAA GAA ACT CCT CCA A 66
Ma-3-41 GAA GCA TCC AAT GGA CCT A 56 60
GCG AAC TCA CAA TAG CGA 54
Contd…
31. Primer Sequence TM (ºC) TA (ºC)
Ma-3-41 CCC GTC CCA TTT CTC A 50 59.8
TTC GTT GTT CAT GGA ATC A 52
Ma-3-50 GGT GGA TGG CTG GGT A 52 59.8
GGA TCC AAG CTT ATC GAG TT 58
Ma-3-55 GGT GCT CTT CGG AGG A 52 58.5
CGT TTA TAT CCA TTC CCA 50
Ma-3-59 GCT TGT CTC TCA CCC ACT C 60 50
ACC GAC TCC CCA ATA GG 54
Ma-3-79 CTG GTC CTT TTC AGT TCA CTC 62 50.3
TAG GCA GCT CCC AAT CA 52
Ma-3-90 GCA CGA AGA GGC ATC AC 54 54.3
GGC CAA ATT TGA TGG ACT 68
32. L M1 M2 M3 M4 M5 M6
a b c d a b c d a b c d a b c d a b c d a b c d
L M7 M8 M9
a b c d a b c d a b c d
a=control, b-d=cryopreserved samples
290 bp
SSR profile obtained with primer pair Ma-3-41
34. Conserving Banana Diversity
for Use in Perpetuity
Bioversity International Funded Project (2009-12)
Agrawal A. and R.K. Tyagi. 2014. Int. J. Innovative Horti. 3(2):115-133.
36. Treatments (4) (3 samples per treatment)
SSR Primers (11)
Ma-SSR-18, Ma-SSR-07, Ma-1-127, Ma-1-18, Ma-2-2, Ma-1-24, Ma-3-41,
Ma-3-55, Ma-3-59, Ma-3-79, Ma-3-90
Plants grown T1 Tissue Culture Controls (not subjected to any
cryotreatment)
T2 PVS2 treated plants (not subjected to LN
treatment)
T3 Plants obtained after LN treatment using
vitrification technique
T4 Plants obtained after LN treatment using droplet-
vitrification
No. of plants : 3 per treatment
Design RBD
Planting Date : July, 2011
Flowering and
Fruiting
Nov., 2012 – Feb, 2014
(First and ratoon crop)
37. T1: In Vitro
conserved
T2 : Cryopreserved using vitrification
T4 :Cryopreserved using droplet- vitrification
Pelipita(ABB)
ANOVA revealed that most of the traits of frozen material were non-significantly
different from non-frozen controls and in vitro conserved material
T3: PVS2 treated
Field Characterization
38. TC-C1 TC-C2 TC-C3 PVS-C1 PVS-C2 PVSC3 V-LN1 V-LN3 V-LN3 DV-LN1 DV-LN2 DV-LN3
SSR profile obtained with primer pair Ma-1-18
1000 bp
500 bp
200 bp
200 bp
EC653573,
Pisang Madu
EC653545,
Pelipita
EC653551,
Pisang
Radjah
41. EC653573, Pisang Madu (AA)
Similarity coefficient based in UPGMA
analysis >80%, except in one sample
of TCC (64%)
42. •20 amplified fragments detected (average 1.8 per primer) among all
control and cryopreserved samples.
•UPGMA (Nei and Li/Dice) analysis at the SSR loci exhibited a high
(>85%) co-efficient of similarity among the control and cryopreserved
samples in the 3 accessions tested.
•In all the three accessions, one tissue culture control sample
exhibited lower co-efficient of similarity (64-85%) as compared to the
rest of the samples.
•All the bands were found to be monomorphic, and variation
observed was due to the absence of the band in a few samples; no
variation was observed based on amplicon size.
•The SSR data is being compared with the morphological data to
conclude if variation observed was in the coding or non-coding region
of the genome.
43. Musa AABp cv Nendran
Cryobank
the
bananas…
But keep a
watch!
Conclusion
44. Genomic
Group
Indigenous accessions (46) Exotic accessions (30)
AAA Borjahaji, Chenkadali,
Dwarf Cavendish, Manoranjitham
Chinese Cavendish, Cocos
Grand Naine, Palang, Pisang Nangka,
Biu Ketip, Red Dacca, Robusta
AAAp and
AAAh
Nendran Akpakpak, Maiden Plantain, Mbi
Egome 1, Obubit Ntanga green
mutant, Plantain no.2, Guineo
AAB Abhar Velchi, Alpan, Amritpani,
Dasaman, Digjowa, Dudhsagar, Giant, H-
2, Honda, Kalibow, Kottavazhai, Ladan,
Ladan Pointed, Soniyal
Blue Torres Strait Island, Cocos,
Kelong Mekintu, Pisang Keling,
Pisang Radjah
ABB Boothi Bale, Booditha Bontha Batheesa,
Cherapunji,
Enna Benian, Karpura Chakkarakeli, Kallu
Monthan, Pacha Bontha Batheesa, Pidi
Monthan, Sommrani Monthan, Sakkar
Chinia, Thozhouvan, Udhyam,
Vellapalayankodan
Cachaco, Kayinja, Pelipita, Fougamou
1
Musa germplasm cryobanked (using meristems) at NBPGR
(As up to 2017)
Contd…
45. Genomic
Group
Indigenous
accessions
Exotic accessions
AB Adukkan, Agniswar,,
Kodappanilla Kunnan,
Somai
AA Matti, Sanna
Chenkadali, Vai Balha
Kaul
Bebek, Bie Yeng, Gu Nin Chio, Gulum,
Kasaska, Kluai Lep Mu Nang, Lidi, NBA 14,
Pisang Buntal, Pisang Mulik, Pisang Tongat,
Pisang Madu, Pitu, Pu-te La-Bum, Sowmuk,
Tjau Lagada
AAw Musa aurantiaca M. acuminata type 2 x, M. ac. ssp
malaccensis type malaccensis; M. ac. type
Kluai Thong Det, M. ac. type Pisang Cici Alas,
M. ac. ssp. banksii
BBw M. balbisiana, Butuhan intermediate apex
Australimusa M. basjoo, M. textilis, M. jackeyi
46. Acknowledgements
• Organizers, CryoSymp2018
• Director General, ICAR, Director(s), NBPGR and
NRCB
• Bart Panis, K.U. Leuven, Belgium
• Bioversity International, Rome, Montpellier,
Leuven & Delhi
(Emile Frison, Stephan Weise, Nicolas Roux,
Prem Mathur, Anne Vezina , Ines van den
Houwe)
• Department of Science and Technology
• S. Uma, R. Selvarajan, M.S. Saraswathi (NRCB),
India
• Anuradha Agrawal and staff/students of TCCU,
NBPGR, India
Thank you!
Projects funded by :
Indian Council of
Agricultural Research
Department of Science and
Technology, GoI
Department of
Biotechnology, GoI
World Bank , NATP
Bioversity International
Global Crop Diversity Trust