This document discusses DNA sequencing methods. It covers the Sanger method, Maxam-Gilbert sequencing, and advanced sequencing techniques. The Sanger method involves amplifying DNA, denaturing it to produce single strands, annealing a primer to the template strand, and dispersing the primed DNA into separate reactions containing chain-terminating nucleotides.

2.  Introduction
 History
 Methods
 Applications
 Advantage and disadvantage

6.  Sanger method or chain termination method
 Maxa-Gilbert sequencing
 Advanced methods

13.  Firstly the DNA to be sequenced has to be
 amplified
 Secondly heat the DNA to denature the DNA to
produce a complementary strand and the
template strand for DNA sequencing
 A primer is then annealed to the 5 end of DNA
mean on the template strand a primer is
attached on 5 end
 The primed DNA is then dispersed equally
among four reaction vessels

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