DNA Fingerprinting or DNA Profiling
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DNA Fingerprinting involves different stages - 1. First is isolation of DNA from target cell. 2. Then treatment of restriction digestion of DNA to generate DNA fragments. 3. Then separation of DNA fragments using Agarose Gel Electrophoresis . 4. Transfer of Agarose Gel DNA bands to nitrocellulose membrane filter. This filter paper after the DNA transfer is known as Southern Blot . 5. Then use of complementary DNA Probe to bind with target DNA . 6. X- Ray film exposure which represent Dark bands of DNA called DNA Fingerprints .
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Dna fingerprinting
DNA fingerprinting.... information to share and learn more... for beginners as well.... must have a look..
DNA Sequencing
The document discusses DNA sequencing, including its history, common methods like Sanger sequencing, the steps involved, challenges and limitations. DNA sequencing determines the order of nucleotides in DNA and has various applications such as identifying genetic diseases, forensics, and studying genome evolution. Sanger sequencing, developed in 1977, works by using chain-terminating dideoxynucleotides during DNA replication to determine the sequence.
Southern blot
Southern Blot is a technique invented by Edwin Southern in the 1970s to locate specific DNA sequences within a larger sample of DNA. It works by cutting DNA with restriction enzymes, separating the fragments via gel electrophoresis, transferring them to a membrane, and using a radioactive or enzyme-labeled probe to bind to complementary DNA sequences, revealing their locations. Southern Blot can be used to map genes, for forensic analysis, and to discover new genes or detect diseases.
Chapter 7 Dna fingerprinting
This chapter discusses DNA fingerprinting, which involves analyzing variable regions of DNA, like VNTRs and STRs, to develop a unique genetic profile that can identify individuals and determine relationships. The key steps are extracting DNA from samples, cutting the DNA into fragments using restriction enzymes, amplifying the fragments via PCR, and then separating the fragments by size via gel electrophoresis to view the band pattern DNA fingerprint. DNA fingerprinting is used in forensics to match crime scene evidence to suspects and establish familial connections through patterns of inheritance.
Dna fingerprinting powerpoint 1
DNA profiling, also known as DNA fingerprinting, is a technique used by forensic scientists to identify individuals by characteristics in their DNA. The process was invented in 1985 by Alec Jeffreys at the University of Leicester. It involves extracting DNA from samples, cutting the DNA into fragments of varying lengths using restriction enzymes, separating the fragments by size using gel electrophoresis, and comparing the resulting patterns to DNA profiles in a database or from other samples. DNA profiling can be used to solve crimes by matching DNA evidence from a crime scene to suspects, and to solve medical problems like paternity testing.
DNA Fingerprinting
DNA fingerprinting is a technique used for identification by extracting and analyzing the base pair pattern of an individual's DNA. It involves isolating DNA from a sample, cutting the DNA into fragments using restriction enzymes, and comparing the fragment patterns on a gel to identify individuals. The main applications of DNA fingerprinting are in solving criminal cases by matching DNA evidence to suspects, diagnosing inherited diseases, and determining biological relationships in areas like paternity testing.
Polymerase Chain Reaction
Polymerase chain reaction (PCR) is an in vitro technique used to amplify a specific region of DNA. It involves repeated cycles of heating and cooling of the DNA sample in the presence of primers, DNA polymerase, and dNTPs. Each cycle doubles the amount of target DNA. Real-time PCR permits both amplification and simultaneous quantification of the target DNA by using fluorescent dyes. It has various applications including disease diagnosis, gene expression analysis, and food testing.
Polymerase Chain Reaction
Introduction, Evolution of PCR, Principle, Instrumentation, Reagents, Primer Designing, DNA polymerase, Components of PCR, PCR Types, Applications
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The document discusses DNA sequencing, including its history, common methods like Sanger sequencing, the steps involved, challenges and limitations. DNA sequencing determines the order of nucleotides in DNA and has various applications such as identifying genetic diseases, forensics, and studying genome evolution. Sanger sequencing, developed in 1977, works by using chain-terminating dideoxynucleotides during DNA replication to determine the sequence.
Southern blot
Southern Blot is a technique invented by Edwin Southern in the 1970s to locate specific DNA sequences within a larger sample of DNA. It works by cutting DNA with restriction enzymes, separating the fragments via gel electrophoresis, transferring them to a membrane, and using a radioactive or enzyme-labeled probe to bind to complementary DNA sequences, revealing their locations. Southern Blot can be used to map genes, for forensic analysis, and to discover new genes or detect diseases.
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Dna fingerprinting powerpoint 1
DNA profiling, also known as DNA fingerprinting, is a technique used by forensic scientists to identify individuals by characteristics in their DNA. The process was invented in 1985 by Alec Jeffreys at the University of Leicester. It involves extracting DNA from samples, cutting the DNA into fragments of varying lengths using restriction enzymes, separating the fragments by size using gel electrophoresis, and comparing the resulting patterns to DNA profiles in a database or from other samples. DNA profiling can be used to solve crimes by matching DNA evidence from a crime scene to suspects, and to solve medical problems like paternity testing.
DNA Fingerprinting
DNA fingerprinting is a technique used for identification by extracting and analyzing the base pair pattern of an individual's DNA. It involves isolating DNA from a sample, cutting the DNA into fragments using restriction enzymes, and comparing the fragment patterns on a gel to identify individuals. The main applications of DNA fingerprinting are in solving criminal cases by matching DNA evidence to suspects, diagnosing inherited diseases, and determining biological relationships in areas like paternity testing.
Polymerase Chain Reaction
Polymerase chain reaction (PCR) is an in vitro technique used to amplify a specific region of DNA. It involves repeated cycles of heating and cooling of the DNA sample in the presence of primers, DNA polymerase, and dNTPs. Each cycle doubles the amount of target DNA. Real-time PCR permits both amplification and simultaneous quantification of the target DNA by using fluorescent dyes. It has various applications including disease diagnosis, gene expression analysis, and food testing.
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Blotting techniques ppt
The document discusses various blotting techniques used to detect DNA, RNA, and proteins. It describes Southern blotting for detecting DNA, Northern blotting for detecting RNA, and Western blotting for detecting proteins. It provides details on the steps involved in each technique, including separating biomolecules by electrophoresis, transferring them to a membrane, and using probes for detection through hybridization or antibody binding.
Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a technique used to amplify small segments of DNA. During PCR, millions of copies of a DNA segment are made in just a few hours by separating the DNA strands, annealing primers, and extending new DNA strands using DNA polymerase. The key components of PCR are the target DNA, primers, DNA polymerase, nucleotides, and buffer solution. The PCR process involves denaturation, annealing, and extension steps that are repeated for multiple cycles to exponentially amplify the target DNA segment. PCR has many applications in basic research, applied research, and medical diagnosis.
DNA fingerprinting- criminology and paternal identification
DNA fingerprinting uses variations in repetitive DNA sequences to uniquely identify individuals. The technique analyzes short tandem repeat polymorphisms that are highly variable between people. DNA profiling has been used in forensic investigations to identify suspects from crime scene DNA samples. It has helped solve many criminal cases by matching DNA evidence to suspects' DNA profiles with a high degree of statistical certainty.
Dna Fingerprinting
DNA fingerprinting is a technique used to analyze an organism's unique DNA composition as a characteristic marker. It works by examining variable number tandem repeats (VNTRs) in DNA, which provide a scientific marker for identity. The two main methods are Restriction Fragment Length Polymorphism (RFLP), which cuts, sorts, and photographs DNA fragments, and Polymerase Chain Reaction (PCR), which amplifies a small DNA sample. DNA fingerprinting is used to identify suspects in criminal cases like murders and rapes, determine parentage in legal disputes, and study non-human samples like wildlife crimes.
DNA Fingerprinting & its techniques by Shiv Kalia (M.Pharma in Analytical Che...
DNA fingerprinting and below mention content widely cover in this presentation
History & Introduction of DNA fingerprinting
How was the first DNA fingerprint produced?
Types of DNA Based Markers
Polymerase Chain Reaction (PCR)
PCR based Methodology of DNA fingerprinting
Electrophoresis
Utility of DNA Based Markers
Various DNA Fingerprinting Techniques Advantages & Disadvantages
Authentication of Various Ayurvedic Herbs by DNA Fingerprinting
Advantages of DNA fingerprinting in Plants
Disadvantages of DNA fingerprinting in Plants
CONCLUSION
Gene Sequencing
Gene Sequencing, a tool to analyze the exact order of nucleotide sequence in the DNA -Deoxyribonucleic Acid.
Focuses on Two methods:
a. Maxam-Gilbert (Chemical Degradation) Method
b. Sanger's Method (Dideoxy Chain termination Method)
polymerase Chain Reaction(PCR)
It is called “polymerase” because the only enzyme used in this reaction is DNA polymerase.
It is called “chain” because the products of the first reaction become substrates of the following one, and so on.
Southern northern and western blotting
Southern, Northern, and Western blotting are techniques to detect specific biomolecules separated by gel electrophoresis. Southern blotting detects DNA using DNA probes, Northern blotting detects RNA using RNA or DNA probes, and Western blotting detects proteins using antibodies. All three techniques involve transferring the separated biomolecules from a gel to a membrane, probing with a labeled detection molecule, washing unbound probes, and detecting signals.
DNA fingerprinting
1) DNA fingerprinting is a technique used to identify individuals by their unique DNA patterns. It analyzes Variable Number Tandem Repeats (VNTRs) in DNA, which vary between people.
2) The Southern blot technique is used to detect and analyze VNTRs. It involves extracting DNA from a sample, cutting it, separating fragments by size, and probing for specific VNTRs.
3) PCR (polymerase chain reaction) is used to amplify small DNA samples. It heats and cools DNA in cycles to make billions of copies of specific DNA regions for analysis.
Dna fingerprinting by laxmee dahal
DNA fingerprinting is a technique used to analyze and compare DNA samples from individuals for identification purposes. It involves extracting DNA from cells, cutting the DNA into fragments using restriction enzymes, separating the fragments by size via gel electrophoresis, and comparing fragment patterns between samples. DNA fingerprinting can be used for paternity testing, criminal investigations by matching crime scene DNA to suspects, and identifying unknown samples. While very accurate, DNA fingerprinting requires high quality samples as contamination can interfere with results. It has helped convict criminals and exonerate innocent suspects.
Southern blot kanchana
Southern blotting is a technique used to detect specific DNA sequences. It involves purifying DNA from cells, cutting the DNA into fragments using restriction enzymes, separating the fragments via gel electrophoresis, transferring the fragments to a membrane, and using a labeled probe to identify fragments that are complementary to the probe. The final step is to visualize the bound probes on the membrane through autoradiography to determine which DNA fragments are present in the sample. Southern blotting can be used for applications like DNA fingerprinting, paternity testing, and identifying genetic mutations or infectious agents.
Techniques of DNA Extraction, Purification and Quantification
Introduction
The overall process…
Uses of isolated genomic DNA
Extraction of DNA from plant material
Components of DNA extraction solutions
Cell Lysis or Cell disruption :
Purification of DNA
CTAB Method
Phenol–chloroform extraction
PROTEINASE K
Salting out
Silica adsorption method
Magnetic beads
FTA Paper
Nucleic acid quantification
Agarose Gel Electrophoresis
UV spectroscopy
DNA quantification using NanoDrop
Dna finger printing
DNA fingerprinting is a technique used to identify individuals by analyzing genetic variations in DNA sequences from samples. It involves 7 steps: extracting DNA from samples, cutting DNA into fragments using restriction enzymes, separating fragments by size using gel electrophoresis, transferring fragments to paper, using radioactive probes to identify specific fragments, detecting hybridized fragments through autoradiography, and comparing fragment patterns to determine genetic matches or exclusions. DNA fingerprinting is used for forensic identification, paternity testing, immigration disputes, and missing persons investigations by comparing an individual's unique DNA profile to other samples.
Pcr
Polymerase chain reaction (PCR) is a technique used to amplify a single copy of a DNA segment across orders of magnitude, generating thousands to millions of copies. PCR involves repeated cycles of heating and cooling of the DNA sample to denature and separate the DNA strands, followed by primer annealing and polymerase extension. This allows for exponential amplification of the target DNA sequence. PCR is commonly used in clinical and research applications such as disease diagnosis, genetic analysis, and forensic identification.
NEXT GENERATION SEQUENCING
Next generation DNA sequencing refers to modern massively parallel DNA sequencing technologies that can generate millions of sequences simultaneously. It involves sequencing small DNA fragments in parallel and then bioinformatics to assemble the sequences. There are several NGS platforms including pyrosequencing, Illumina sequencing, and Ion Torrent sequencing which use different chemistries to sequence DNA in a massively parallel fashion, enabling large-scale genome and transcriptome sequencing. These technologies have significantly reduced the cost of DNA sequencing.
Namrata singh -recombinant dna technology
Recombinant DNA technology ( also known as genetic engineering) is the set of techniques that enable the DNA to be identified, isolated and recombined so that new characteristics can be introduced into the genome of organism. It was largely the work of Paul Berg, Herbert W. Boyer and Stanley N Cohen, although many other scientists made important contributions to the new technology as well.One important aspect of Recombinant DNA Technology is DNA Cloning.
Recombinant dna technology
This document discusses recombinant DNA technology and its applications. It covers key steps like cloning vectors, restriction modification systems, DNA ligases, transformation, and screening cDNA libraries to identify genes. Examples are given of recombinant proteins used as drugs to treat diseases like growth hormone deficiency, diabetes, and hemophilia. The last section briefly discusses genetic modification of animals and plants, and debates around genetically engineered salmon.
Dna extraction
The document discusses various DNA extraction methods from biological samples. It describes organic extraction, non-organic extraction, Chelex extraction, and FTA paper methods. Organic extraction uses phenol-chloroform to remove proteins and isolate DNA, while non-organic extraction uses high salt concentrations. Chelex extraction uses an ion exchange resin to remove ions that degrade DNA. FTA paper allows samples to be stored at room temperature. The minimum DNA quantities needed for different techniques is also discussed.
NEXT GENERATION SEQUENCING
This presentation is explains about the genome sequencing, its traditional method and modern method. This basically focus on Next Generation Sequencing and its types.
Dna sequencing
DNA sequencing determines the order of nucleotide bases in a DNA sample. The Sanger method, also known as dideoxy or chain termination method, was the first widely used DNA sequencing technique. It involves DNA polymerase, dNTPs, a primer, and ddNTPs to terminate DNA strand extension. The fragments are separated by gel electrophoresis and the sequence is read. Next-generation sequencing methods like pyrosequencing do not require electrophoresis or fragment separation. They detect incorporated nucleotides in real-time. Nanopore sequencing detects changes in electrical current as single-stranded DNA passes through a nanopore protein.
Blotting Techniques.pptx
Southern blotting is a technique used to detect specific DNA sequences in a DNA sample. It involves digesting DNA samples with restriction enzymes, separating the fragments via gel electrophoresis, transferring the fragments to a membrane, and then detecting specific sequences using DNA probes. Key steps include purifying DNA from samples, fragmenting DNA with restriction enzymes, separating fragments by size using gel electrophoresis, transferring DNA from the gel to a membrane, and detecting fragments using hybridization with labeled DNA probes. This allows for the identification and analysis of specific DNA sequences.
DNA PROFILING
DNA profiling is a technique used by scientists to distinguish individuals using DNA samples. It involves breaking down cells to extract DNA, amplifying small DNA samples, cutting the DNA into fragments using restriction enzymes, separating the fragments by size using gel electrophoresis to produce a fluorescent image of bands, and analyzing the pattern of fragment distribution.
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Blotting techniques ppt
The document discusses various blotting techniques used to detect DNA, RNA, and proteins. It describes Southern blotting for detecting DNA, Northern blotting for detecting RNA, and Western blotting for detecting proteins. It provides details on the steps involved in each technique, including separating biomolecules by electrophoresis, transferring them to a membrane, and using probes for detection through hybridization or antibody binding.
Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a technique used to amplify small segments of DNA. During PCR, millions of copies of a DNA segment are made in just a few hours by separating the DNA strands, annealing primers, and extending new DNA strands using DNA polymerase. The key components of PCR are the target DNA, primers, DNA polymerase, nucleotides, and buffer solution. The PCR process involves denaturation, annealing, and extension steps that are repeated for multiple cycles to exponentially amplify the target DNA segment. PCR has many applications in basic research, applied research, and medical diagnosis.
DNA fingerprinting- criminology and paternal identification
DNA fingerprinting uses variations in repetitive DNA sequences to uniquely identify individuals. The technique analyzes short tandem repeat polymorphisms that are highly variable between people. DNA profiling has been used in forensic investigations to identify suspects from crime scene DNA samples. It has helped solve many criminal cases by matching DNA evidence to suspects' DNA profiles with a high degree of statistical certainty.
Dna Fingerprinting
DNA fingerprinting is a technique used to analyze an organism's unique DNA composition as a characteristic marker. It works by examining variable number tandem repeats (VNTRs) in DNA, which provide a scientific marker for identity. The two main methods are Restriction Fragment Length Polymorphism (RFLP), which cuts, sorts, and photographs DNA fragments, and Polymerase Chain Reaction (PCR), which amplifies a small DNA sample. DNA fingerprinting is used to identify suspects in criminal cases like murders and rapes, determine parentage in legal disputes, and study non-human samples like wildlife crimes.
DNA Fingerprinting & its techniques by Shiv Kalia (M.Pharma in Analytical Che...
DNA fingerprinting and below mention content widely cover in this presentation
History & Introduction of DNA fingerprinting
How was the first DNA fingerprint produced?
Types of DNA Based Markers
Polymerase Chain Reaction (PCR)
PCR based Methodology of DNA fingerprinting
Electrophoresis
Utility of DNA Based Markers
Various DNA Fingerprinting Techniques Advantages & Disadvantages
Authentication of Various Ayurvedic Herbs by DNA Fingerprinting
Advantages of DNA fingerprinting in Plants
Disadvantages of DNA fingerprinting in Plants
CONCLUSION
Gene Sequencing
Gene Sequencing, a tool to analyze the exact order of nucleotide sequence in the DNA -Deoxyribonucleic Acid.
Focuses on Two methods:
a. Maxam-Gilbert (Chemical Degradation) Method
b. Sanger's Method (Dideoxy Chain termination Method)
polymerase Chain Reaction(PCR)
It is called “polymerase” because the only enzyme used in this reaction is DNA polymerase.
It is called “chain” because the products of the first reaction become substrates of the following one, and so on.
Southern northern and western blotting
Southern, Northern, and Western blotting are techniques to detect specific biomolecules separated by gel electrophoresis. Southern blotting detects DNA using DNA probes, Northern blotting detects RNA using RNA or DNA probes, and Western blotting detects proteins using antibodies. All three techniques involve transferring the separated biomolecules from a gel to a membrane, probing with a labeled detection molecule, washing unbound probes, and detecting signals.
DNA fingerprinting
1) DNA fingerprinting is a technique used to identify individuals by their unique DNA patterns. It analyzes Variable Number Tandem Repeats (VNTRs) in DNA, which vary between people.
2) The Southern blot technique is used to detect and analyze VNTRs. It involves extracting DNA from a sample, cutting it, separating fragments by size, and probing for specific VNTRs.
3) PCR (polymerase chain reaction) is used to amplify small DNA samples. It heats and cools DNA in cycles to make billions of copies of specific DNA regions for analysis.
Dna fingerprinting by laxmee dahal
DNA fingerprinting is a technique used to analyze and compare DNA samples from individuals for identification purposes. It involves extracting DNA from cells, cutting the DNA into fragments using restriction enzymes, separating the fragments by size via gel electrophoresis, and comparing fragment patterns between samples. DNA fingerprinting can be used for paternity testing, criminal investigations by matching crime scene DNA to suspects, and identifying unknown samples. While very accurate, DNA fingerprinting requires high quality samples as contamination can interfere with results. It has helped convict criminals and exonerate innocent suspects.
Southern blot kanchana
Southern blotting is a technique used to detect specific DNA sequences. It involves purifying DNA from cells, cutting the DNA into fragments using restriction enzymes, separating the fragments via gel electrophoresis, transferring the fragments to a membrane, and using a labeled probe to identify fragments that are complementary to the probe. The final step is to visualize the bound probes on the membrane through autoradiography to determine which DNA fragments are present in the sample. Southern blotting can be used for applications like DNA fingerprinting, paternity testing, and identifying genetic mutations or infectious agents.
Techniques of DNA Extraction, Purification and Quantification
Introduction
The overall process…
Uses of isolated genomic DNA
Extraction of DNA from plant material
Components of DNA extraction solutions
Cell Lysis or Cell disruption :
Purification of DNA
CTAB Method
Phenol–chloroform extraction
PROTEINASE K
Salting out
Silica adsorption method
Magnetic beads
FTA Paper
Nucleic acid quantification
Agarose Gel Electrophoresis
UV spectroscopy
DNA quantification using NanoDrop
Dna finger printing
DNA fingerprinting is a technique used to identify individuals by analyzing genetic variations in DNA sequences from samples. It involves 7 steps: extracting DNA from samples, cutting DNA into fragments using restriction enzymes, separating fragments by size using gel electrophoresis, transferring fragments to paper, using radioactive probes to identify specific fragments, detecting hybridized fragments through autoradiography, and comparing fragment patterns to determine genetic matches or exclusions. DNA fingerprinting is used for forensic identification, paternity testing, immigration disputes, and missing persons investigations by comparing an individual's unique DNA profile to other samples.
Pcr
Polymerase chain reaction (PCR) is a technique used to amplify a single copy of a DNA segment across orders of magnitude, generating thousands to millions of copies. PCR involves repeated cycles of heating and cooling of the DNA sample to denature and separate the DNA strands, followed by primer annealing and polymerase extension. This allows for exponential amplification of the target DNA sequence. PCR is commonly used in clinical and research applications such as disease diagnosis, genetic analysis, and forensic identification.
NEXT GENERATION SEQUENCING
Next generation DNA sequencing refers to modern massively parallel DNA sequencing technologies that can generate millions of sequences simultaneously. It involves sequencing small DNA fragments in parallel and then bioinformatics to assemble the sequences. There are several NGS platforms including pyrosequencing, Illumina sequencing, and Ion Torrent sequencing which use different chemistries to sequence DNA in a massively parallel fashion, enabling large-scale genome and transcriptome sequencing. These technologies have significantly reduced the cost of DNA sequencing.
Namrata singh -recombinant dna technology
Recombinant DNA technology ( also known as genetic engineering) is the set of techniques that enable the DNA to be identified, isolated and recombined so that new characteristics can be introduced into the genome of organism. It was largely the work of Paul Berg, Herbert W. Boyer and Stanley N Cohen, although many other scientists made important contributions to the new technology as well.One important aspect of Recombinant DNA Technology is DNA Cloning.
Recombinant dna technology
This document discusses recombinant DNA technology and its applications. It covers key steps like cloning vectors, restriction modification systems, DNA ligases, transformation, and screening cDNA libraries to identify genes. Examples are given of recombinant proteins used as drugs to treat diseases like growth hormone deficiency, diabetes, and hemophilia. The last section briefly discusses genetic modification of animals and plants, and debates around genetically engineered salmon.
Dna extraction
The document discusses various DNA extraction methods from biological samples. It describes organic extraction, non-organic extraction, Chelex extraction, and FTA paper methods. Organic extraction uses phenol-chloroform to remove proteins and isolate DNA, while non-organic extraction uses high salt concentrations. Chelex extraction uses an ion exchange resin to remove ions that degrade DNA. FTA paper allows samples to be stored at room temperature. The minimum DNA quantities needed for different techniques is also discussed.
NEXT GENERATION SEQUENCING
This presentation is explains about the genome sequencing, its traditional method and modern method. This basically focus on Next Generation Sequencing and its types.
Dna sequencing
DNA sequencing determines the order of nucleotide bases in a DNA sample. The Sanger method, also known as dideoxy or chain termination method, was the first widely used DNA sequencing technique. It involves DNA polymerase, dNTPs, a primer, and ddNTPs to terminate DNA strand extension. The fragments are separated by gel electrophoresis and the sequence is read. Next-generation sequencing methods like pyrosequencing do not require electrophoresis or fragment separation. They detect incorporated nucleotides in real-time. Nanopore sequencing detects changes in electrical current as single-stranded DNA passes through a nanopore protein.
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DNA fingerprinting- criminology and paternal identification
DNA fingerprinting- criminology and paternal identification
DNA Fingerprinting & its techniques by Shiv Kalia (M.Pharma in Analytical Che...
DNA Fingerprinting & its techniques by Shiv Kalia (M.Pharma in Analytical Che...
Techniques of DNA Extraction, Purification and Quantification
Techniques of DNA Extraction, Purification and Quantification
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Blotting Techniques.pptx
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DNA PROFILING
DNA profiling is a technique used by scientists to distinguish individuals using DNA samples. It involves breaking down cells to extract DNA, amplifying small DNA samples, cutting the DNA into fragments using restriction enzymes, separating the fragments by size using gel electrophoresis to produce a fluorescent image of bands, and analyzing the pattern of fragment distribution.
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Dna fingerprinting- Logan Briggs
DNA fingerprinting is a process used to identify individuals by their unique DNA patterns. It involves cutting DNA into fragments using restriction enzymes, separating the fragments via gel electrophoresis, transferring them to a membrane, and detecting specific DNA sequences using radioactive probes. When exposed to X-ray film, the probe-hybridized fragments produce a pattern of bands that serves as an individual's unique DNA fingerprint. DNA fingerprinting has applications in criminal investigations, genetic trait and disease analysis, animal breeding, and disease tracking and origin identification.
Souther and northern blotting systems
Southern blotting and Northern blotting are techniques used to detect specific DNA and RNA sequences. Southern blotting involves transferring DNA fragments separated by gel electrophoresis to a membrane, then using a labeled probe to identify fragments by hybridization. Northern blotting follows similar steps but detects RNA, requiring formaldehyde treatment to denature RNA. Both techniques allow identification of specific molecules in complex mixtures.
Blotting techniques
1. The document discusses various blotting techniques including Southern blotting, Northern blotting, and Western blotting. It provides a brief history and overview of each technique.
2. Southern blotting is used to detect DNA, Northern blotting detects RNA, and Western blotting detects proteins. The techniques involve separating biomolecules by electrophoresis, transferring them to a membrane, then using a probe for detection.
3. The document outlines the basic methodology for each type of blotting. This includes separation, transfer to a membrane, blocking, hybridization with a probe, washing, and detection. Each technique allows for the analysis of biomolecules and has various applications in research.
Bloting techniques.pptx
Blotting techniques allow the transfer of DNA, RNA, or proteins from a gel to a membrane for detection. Southern blotting detects DNA using separation, transfer, and hybridization with a DNA probe. Northern blotting detects RNA and was developed at Stanford University. Western blotting identifies specific proteins by separating them using SDS-PAGE gel electrophoresis, transferring them to a membrane, and detecting them using labeled antibodies in an antigen-antibody reaction. These techniques have applications in gene discovery, mapping, diagnostics, and more.
Northern Blotting PPT ( The transfer of RNA to membrane)
The northern blot technique allows researchers to detect specific RNA molecules and study gene expression. It was developed in 1977 at Stanford University as an adaptation of the southern blot which detects DNA. The northern blot involves separating RNA fragments via gel electrophoresis, transferring them to a membrane, then using a labeled probe to detect the RNA of interest via autoradiography or fluorescence. It provides a way to analyze RNA expression and has been an important tool in molecular biology research.
Blotting southern,northern, western techniques
This document describes various blotting techniques used to detect specific nucleic acids or proteins in samples, including Southern blotting, Northern blotting, and Western blotting. It provides an overview of the basic principles and steps involved in each technique, such as separating biomolecules by size, transferring them to a membrane, hybridizing probes, washing unbound probes, and detecting bound probes. The techniques allow researchers to analyze gene expression, detect mutations, and study proteins.
Blotting techniques
This document summarizes several blotting techniques used in molecular biology to detect biomolecules like DNA, RNA, and proteins. Southern blotting detects specific DNA sequences, Northern blotting detects RNA, and Western blotting detects proteins. Eastern blotting detects post-translational modifications to proteins. Dot blotting directly applies biomolecule samples to a membrane as dots to detect presence or absence without separation by size. These techniques involve transferring molecules to a membrane, probing with a labeled probe, washing, and detecting hybridized probes.
southern blotting (used to detect the presence of a particular piece of DNA i...
The technique was developed by E.M. Southern in 1975.
The Southern blot is used to detect the presence of a particular piece of DNA in a sample
mujahid hussain, Department of Botany, University of Sargodha, Sargodha, Punjab, Pakistan
Southern blotting
these slides can easily give better understanding to the reader about the Steps involved in Southern Blotting.
VBC-321_BLOTTING_TECHNIQUES.pptx
1. Blotting techniques such as Southern, Northern, and Western blotting allow for the transfer of DNA, RNA, and proteins from a gel to a membrane for detection.
2. The Southern blot detects DNA using hybridization with a labeled probe. The Northern blot detects RNA and the Western blot detects proteins using antibodies.
3. These techniques separate biomolecules by size then transfer and detect them on a membrane using probes or antibodies, allowing analysis of complex samples.
Southern blotting
Southern blotting and Northern blotting are techniques used to detect specific DNA and RNA sequences. Southern blotting involves extracting DNA from cells, cutting the DNA into fragments using restriction enzymes, separating the fragments by size using gel electrophoresis, transferring the fragments to a membrane, and using a labeled probe to detect complementary DNA sequences via hybridization. Northern blotting uses a similar process to detect specific RNA sequences, and is used to study gene expression and RNA processing. Both techniques allow detection of specific sequences among a large mixture of nucleic acids.
DNA sequencing
This document discusses various methods for DNA sequencing, including both early chemical methods and more recent next-generation sequencing technologies. It describes Frederick Sanger's chain termination method from 1977, which uses DNA polymerase, dNTPs, and ddNTPs. It also describes Maxam-Gilbert sequencing from the same year, which uses chemical cleavage. Next-generation methods discussed include pyrosequencing, sequencing by ligation, sequencing by synthesis, and ion semiconductor sequencing. The document compares the different methods based on read length, accuracy, throughput, run time, and cost.
VBC-321_BLOTTING_TECHNIQUES and gel electrophoresis
Southern blotting is a technique used to detect specific DNA sequences. It involves separating DNA fragments by size via gel electrophoresis, transferring them to a membrane, then using a labeled probe to hybridize to complementary DNA sequences. This allows visualization of the location and size of DNA fragments containing the probe sequence. Key steps are digesting DNA, electrophoretic sorting, denaturing and transferring DNA to a membrane, blocking with excess DNA, washing unbound probe, then autoradiography to detect the bound probe and identify DNA fragments of interest.
Dna finger printing
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Blots.ppt
Southern blotting is a technique used to detect specific DNA sequences within genomes. It involves digesting genomic DNA with restriction enzymes, separating the fragments via gel electrophoresis, transferring the DNA to a membrane, and using a radioactive probe to hybridize to complementary sequences on the membrane. This allows researchers to visualize specific DNA fragments and analyze their presence, absence, size, and any alterations like mutations.
Blotting techniques
blotting techniques, western blotting, eastern blotting, northern blotting, southern blotting, immunoblotting, Far-eastern blotting, far western blotting
Dna fingerprinting
DNA fingerprinting is a technique used to identify individuals by analyzing patterns in their DNA. It involves extracting DNA from a sample, cutting the DNA into fragments of varying lengths using restriction enzymes, sorting the fragments by size via electrophoresis, and then transferring and labeling the fragments to produce a unique pattern or "fingerprint" that can be used for criminal investigations, diagnosing genetic disorders, or other identification purposes. The process involves 6 main steps: extracting DNA, cutting and sorting fragments, transferring DNA to a nylon sheet, adding probes, washing excess probes, and reading the fingerprint pattern.
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This is part 1 of my Java Learning Journey. This Contains Custom methods, classes, constructors, packages, multithreading , try- catch block, finally block and more.
How to Manage Your Lost Opportunities in Odoo 17 CRM
Odoo 17 CRM allows us to track why we lose sales opportunities with "Lost Reasons." This helps analyze our sales process and identify areas for improvement. Here's how to configure lost reasons in Odoo 17 CRM
Executive Directors Chat Leveraging AI for Diversity, Equity, and Inclusion
Let’s explore the intersection of technology and equity in the final session of our DEI series. Discover how AI tools, like ChatGPT, can be used to support and enhance your nonprofit's DEI initiatives. Participants will gain insights into practical AI applications and get tips for leveraging technology to advance their DEI goals.
South African Journal of Science: Writing with integrity workshop (2024)
South African Journal of Science: Writing with integrity workshop (2024)Academy of Science of South Africa
A workshop hosted by the South African Journal of Science aimed at postgraduate students and early career researchers with little or no experience in writing and publishing journal articles.Advanced Java[Extra Concepts, Not Difficult].docx
This is part 2 of my Java Learning Journey. This contains Hashing, ArrayList, LinkedList, Date and Time Classes, Calendar Class and more.
DRUGS AND ITS classification slide share
Any substance (other than food) that is used to prevent, diagnose, treat, or relieve symptoms of a
disease or abnormal condition
Chapter 4 - Islamic Financial Institutions in Malaysia.pptx
Chapter 4 - Islamic Financial Institutions in Malaysia.pptxMohd Adib Abd Muin, Senior Lecturer at Universiti Utara Malaysia
This slide is special for master students (MIBS & MIFB) in UUM. Also useful for readers who are interested in the topic of contemporary Islamic banking.
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...Dr. Vinod Kumar Kanvaria
Exploiting Artificial Intelligence for Empowering Researchers and Faculty,
International FDP on Fundamentals of Research in Social Sciences
at Integral University, Lucknow, 06.06.2024
By Dr. Vinod Kumar KanvariaPCOS corelations and management through Ayurveda.
This presentation includes basic of PCOS their pathology and treatment and also Ayurveda correlation of PCOS and Ayurvedic line of treatment mentioned in classics.
ANATOMY AND BIOMECHANICS OF HIP JOINT.pdf
it describes the bony anatomy including the femoral head , acetabulum, labrum . also discusses the capsule , ligaments . muscle that act on the hip joint and the range of motion are outlined. factors affecting hip joint stability and weight transmission through the joint are summarized.
How to Fix the Import Error in the Odoo 17
An import error occurs when a program fails to import a module or library, disrupting its execution. In languages like Python, this issue arises when the specified module cannot be found or accessed, hindering the program's functionality. Resolving import errors is crucial for maintaining smooth software operation and uninterrupted development processes.
CACJapan - GROUP Presentation 1- Wk 4.pdf
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
The History of Stoke Newington Street Names
Presented at the Stoke Newington Literary Festival on 9th June 2024
www.StokeNewingtonHistory.com
How to Build a Module in Odoo 17 Using the Scaffold Method
Odoo provides an option for creating a module by using a single line command. By using this command the user can make a whole structure of a module. It is very easy for a beginner to make a module. There is no need to make each file manually. This slide will show how to create a module using the scaffold method.
The Diamonds of 2023-2024 in the IGRA collection
A review of the growth of the Israel Genealogy Research Association Database Collection for the last 12 months. Our collection is now passed the 3 million mark and still growing. See which archives have contributed the most. See the different types of records we have, and which years have had records added. You can also see what we have for the future.
Pollock and Snow "DEIA in the Scholarly Landscape, Session One: Setting Expec...
Pollock and Snow "DEIA in the Scholarly Landscape, Session One: Setting Expec...National Information Standards Organization (NISO)
This presentation was provided by Steph Pollock of The American Psychological Association’s Journals Program, and Damita Snow, of The American Society of Civil Engineers (ASCE), for the initial session of NISO's 2024 Training Series "DEIA in the Scholarly Landscape." Session One: 'Setting Expectations: a DEIA Primer,' was held June 6, 2024.Recently uploaded (20)
How to Manage Your Lost Opportunities in Odoo 17 CRM
How to Manage Your Lost Opportunities in Odoo 17 CRM
Executive Directors Chat Leveraging AI for Diversity, Equity, and Inclusion
Executive Directors Chat Leveraging AI for Diversity, Equity, and Inclusion
South African Journal of Science: Writing with integrity workshop (2024)
South African Journal of Science: Writing with integrity workshop (2024)
Chapter 4 - Islamic Financial Institutions in Malaysia.pptx
Chapter 4 - Islamic Financial Institutions in Malaysia.pptx
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...
How to Build a Module in Odoo 17 Using the Scaffold Method
How to Build a Module in Odoo 17 Using the Scaffold Method
Pollock and Snow "DEIA in the Scholarly Landscape, Session One: Setting Expec...
Pollock and Snow "DEIA in the Scholarly Landscape, Session One: Setting Expec...
DNA Fingerprinting or DNA Profiling
- 1. Biology – let’s simplify
- 2. DNA FINGERPRINTING STEP-1. DNA EXTRACTION : BLOOD SEMEN CHEEK SWABS CHEMICAL TREATMENT PURE DNA
- 3. STEP- 2. PCR Amplification of the sample.
- 4. CUTTING : RESTRICTION ENZYMES Molecular ‘scissors’ : Cut the DNA at specific site to isolate the VNTRs. E.g.- EcoRI cuts at 5’…GAATTC…3’
- 5. SORTING : GEL ELECTROPHORESIS • Agarose : Linear polymer extracted from seaweed. • Migration of DNA in Agarose Gel dependent on four factors :- • Molecular size of DNA • Conformation of DNA • Agarose concentration • Applied current
- 6. GEL ELECTROPHORESIS STEPS DNA is negatively charge molecule. Smallest DNA Fragments move fast. Largest DNA Fragments move slowly.
- 7. SOUTHERN BLOTTING • DNA is ‘blotted’ out of the Agarose gel on to a piece of nylon membrane by Capillary action. • ‘Unzipped’ to produce single strands of DNA.
- 8. PROBING : RADIOACTIVE DNA PROBE • Nylon membrane is incubated with radioactive probes. • Probes: Minisatellite DNA tagged with radioactive phosphorous.
- 9. Dna fingerprint : X-RAY FILM EXPOSURE • Radioactive DNA bands visualized by exposing the nylon membrane to X-ray film. • When exposed to radioactivity a pattern of more than 30 dark bands appeared on the film.