- DNA is the genetic material that is found in the nucleus and mitochondria of cells. It carries the instructions needed for building and maintaining all living things.
- Experiments in the 1950s, including those by Hershey and Chase using bacteriophages, provided conclusive evidence that DNA—not proteins or RNA—is the genetic material. They showed that only DNA from the phages entered bacterial cells to direct the production of new phages.
- DNA is composed of nucleotides with a sugar (deoxyribose), phosphate group, and one of four nitrogenous bases (adenine, guanine, cytosine, thymine). The structure of DNA was discovered by Watson and Crick in 1953 to be
Ribozymes (ribonucleic acid enzymes) are RNA molecules that are capable of catalyzing specific biochemical reactions, similar to the action of protein enzymes.
Forces responsible for A-, B- and Z-DNA: Structures, conformations, classific...St Xaviers
Another project out.
This is a brief and slightly in depth explanation of the DNA conformations; Right handed A-DNA and B-DNA and left handed Z-DNA. With pictorial explanation via slides which can be used as notes for College for Degree students in Biochemistry for General, hons and medical students.
Good luck fellow aspirants
ATP synthase—also called FoF1 ATPase is the universal protein that terminates oxidative phosphorylation by synthesizing ATP from ADP and phosphate.
ATP Synthase is one of the most important enzymes found in the mitochondria of cells
Ribozymes (ribonucleic acid enzymes) are RNA molecules that are capable of catalyzing specific biochemical reactions, similar to the action of protein enzymes.
Forces responsible for A-, B- and Z-DNA: Structures, conformations, classific...St Xaviers
Another project out.
This is a brief and slightly in depth explanation of the DNA conformations; Right handed A-DNA and B-DNA and left handed Z-DNA. With pictorial explanation via slides which can be used as notes for College for Degree students in Biochemistry for General, hons and medical students.
Good luck fellow aspirants
ATP synthase—also called FoF1 ATPase is the universal protein that terminates oxidative phosphorylation by synthesizing ATP from ADP and phosphate.
ATP Synthase is one of the most important enzymes found in the mitochondria of cells
A DNA molecule is composed of two chains of nucleotides that wind about each other to resemble a twisted ladder. The sides of the ladder are made up of sugars and phosphates, and the rungs are formed by bonded pairs of nitrogenous bases. These bases are adenine (A), guanine (G), cytosine (C), and thymine (T).
Transduction is the process by which foreign DNA is introduced into a cell by a virus or viral vector. An example is the viral transfer of DNA from one bacterium to another and hence an example of horizontal gene transfer.
DNA replication is the process by which DNA makes a copy of itself during cell division.The separation of the two single strands of DNA creates a 'Y' shape called a replication 'fork'. The two separated strands will act as templates for making the new strands of DNA.
A drink is a liquid intended for human consumption. In addition to their basic function of satisfying thirst, drinks play important roles in human culture. Common types of drinks include plain drinking water, milk, juice and soft drinks.
RNA is a ribonucleic acid that helps in the synthesis of proteins in our body. This nucleic acid is responsible for the production of new cells in the human body. It is usually obtained from the DNA molecule.
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June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
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The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
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students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
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2. • The progeny of organism develops characters similar to that
organism
• The resemblance of offspring to their parents depends on
the precise transmission of principle component from one
generation to the next
• That component is- The Genetic Material
3. The genetic material of a
cell or an organism refers to
those materials found in
the nucleus, mitochondria
and cytoplasm, which play
a fundamental role in
determining the structure
and nature of cell
substances, and capable of
self-propagating and
variation.
5. • Must carry information
– Cracking the genetic code
• Must self replicate
– DNA replication
• Must allow for
informationto change
– Mutation
• Must govern the
expressionof the
phenotype
– Gene function
7. • The process of identification of genetic material began in
1928 with experiments of Griffith and concluded in 1952
with the studies of Hershey and Chase.
• Between these two experiments other three scientists,
Avery, Macloed and McCarty were did an experiment to
identify the genetic material.
8. • 1952 Alfred Hershey and
Martha Chase
• New York scientists
• Performed an experiment that
settled the controversy
• Proved that DNA carries the
genetic material
9. • Bacteriophage: a virus that
infects bacteria.
• When Bacteriophages infect
bacterial cells they produce
more viruses.
• The viruses are released
when the bacterial cells
rupture.
10. • How does the bacteriophage
reprogram the bacterial cell
to make more viruses?
• Does the bacteriophage
DNA, the protein, or both
give instructions to the
bacteria?
11. • Bacteriophage DNA was labeled with radioactive phosphorus
(32P)
• Bacteriophage protein was labeled with radioactive sulphur
(35S)
• only the bacteriophage DNA (as indicated by the 32P) entered
the bacteria and was used to produce more bacteriophage
• Conclusion: DNA is the genetic material
12. • The final evidence that DNA transmits genetic information
was provided by Hershey and Chase in 1952.
• They experimented with T2 bacteriophages, viruses that
attack bacteria.
13.
14.
15. • 1928
• Fredrick Griffith
• Bacteriologist
• Trying to prepare a
vaccine against
pneumonia
16.
17. • Two types, or strains, of S. pneumoniae
• First strain is enclosed in a capsule composed of
polysaccharides.
• Capsule protects the bacterium from the body ’ s
defense system.
• Forms smooth-edges (S) when grown in a petri dish
• Helps make the microorganism virulent, or able to
cause disease.
18. • Second strain lacks the polysaccharide capsule
and does not cause disease.
• Forms rough-edges (R) when grown in a petri dish
19. • The harmless R bacteria had changed and
became virulent S bacteria.
• Transformation is a change in genotype
caused when cells take up foreign material.
20. • Based on these observations he concluded that some of
the cells of type II R had changed into type III S due to
influence of dead type III S cells
• He called this phenomenon as transformation
• Principle Component of type III S cells which induced
the conversion of type II R cells into type III S was
named transforming principle.
22. • DNA is the blueprint of life.
• It contains information on which life is based.
• RNA is the other type of nucleic acid which is equally imp
for life.
• Broadly speaking, one can say that if DNA is the
information then RNA is an intermediate message based
on that information.
• Both these nucleic acids are polymer of basic unit which
are called nucleotides.
BUILDING BLOCKS OF NUCLEIC ACIDS
23. • Friedrich Miescher discovered nucleic acids in 1869.
• They were called nuclein.
• Richard Altmann in 1889 discovered that nuclein has acidic
properties and it was named as nucleic acid.
25. FEATURE DNA RNA
Definition It is a long polymer. It has a
deoxyribose and phosphate
backbone having four
distinct bases: A,T,G,C
Is a polymer with a ribose and
phosphate backbone with four
varying bases:U,C,A,G
Location located in the nucleus of a
cell and in the mitochondria.
found in the cytoplasm, nucleus, and
in the ribosome
Sugar 2-deoxyribose. Ribose.
Function transmission of genetic
information.
transmission of the genetic code that
is necessary for the protein creation
from the nucleus to the ribosome.
Predominant
Structure
double-stranded molecule
that has a long chain of
nucleotides.
single-stranded molecule which has
a shorter chain of nucleotides
Propagation self-replicating. It is synthesized from DNA when
required
DIFFERENCE BETWEEN DNA&RNA
27. • 1953—James Watson and Francis Crick
determined the structure of the DNA
molecule to be a double helix
twisted around
• 2 strands of nucleotides
each other
28.
29. • They proposed that DNA
as a right handed double
helix with two poly
nucleotide chains are
coiled about one another
in a spiral.
30.
31. • Rosalind Franklin contributed
to this discovery by producing
an X-ray crystallographic
picture of DNA
• Determined helix was a uniform diameter and composed
of 2 strands of stacked nucleotides
DNA = Tightly wound helix
32.
33. • Nucleotides are the monomeric units that
make up DNA
3 main parts:
5 carbon sugar—deoxyribose
Phosphategroup
Nitrogenous base
34. The nucleoside is a single molecule and does not have other variants
Two examples of nucleosides
Nucleosides
35. O P O N
Nitrogenous
base
O
CH2
O
O
Pentose
sugar
Phosphate
group
Diagram of nucleotide showing the three components.
36. Nucleoside
• Base
• Sugar
• doesn’t involve in constructing
DNA
• The nucleoside is a single
molecule and does not have
other variants
Nucleotide
• Base
• Sugar
• Phosphoric acid
• Based on the number of
phosphate groups the
nucleotides can be divided into
nucleotide monophosphate,
nucleotide diphosphate and
nucleotide triphosphate
DIFFERENCE BETWEEN NUCLEOSIDE & NUCLEOTIDE
41. • Generate and store energy in ATP form
(eukaryote cells).
• Have DNA and mtDNA is double
stranded circular molecule.
• Circular mtDNA has 37 genes. 1 part (D-
loop) does ’ t contain genes. Why?
Becomes displaced during replication.
44. Chargaff’s base pairing rule:
Percent of adenine = percent of thymine(A=T)
Percent of cytosine = percent of guanine (C=G)
A+G = T+C (or purines = pyrimidines)
45. • Erwin Chargaff showed the amounts of the four bases on
DNA ( A,T,C,G)
In a body or somatic cell:
A = 30.3%
T = 30.3%
G = 19.5%
C = 19.9%
T A
G C
46. Three major forms:
B-DNA
A-DNA
Z-DNA
B-DNA
is biologically THE MOST COMMON
It is a helix meaning thatit has a Right handed, or clockwise, spiral.
Ideal B-DNA has10 base pair per turn
So each base is twisted 36o relative to adjacentbases.
Base pair are 0.34 nm apart.
So completerotation of moleculeis 3.4 nm.
Axis passes through middleof each basepairs.
47.
48. Minor Groove is Narrow, Shallow.
Major Groove is Wide, Deep.
This structure exists when plenty
of water surrounds molecule and
there is no unusual base sequence
in DNA-Condition that are likely to
be present in the cells.
B-DNA structure is most stable
configuration for a random
sequence of nucleotides under
physiological condition.
49. A-DNA
Right-handed helix
Wider and flatter than B-DNA
11 bp per turn
Its bases are tilted away from
main axis of molecule
Narrow Deep major Groove and
Broad, Shallow minor Groove.
Observed when less water is
present. i.e.Dehydrating condition.
A-DNA has been observed in
two context:
• Active site of DNA polymerase
(~3bp)
• Gram (+) bacteria undergoing
sporulation
50. Z-DNA
• A left-handed helix
• Seen in Conditionof High salt concentration.
• In this form sugar-phosphatebackboneszigzag back
• and forth, giving rise to the name Z-DNA (for zigzag).
• 12 base pairs per turn.
• A deep Minor Groove.
• No Discernible Major Groove.
• Part of some active genes form
Z-DNA, suggesting that Z-DNA
may play a role in regulating
gene transcription.
51. Property B-DNA A-DNA Z-DNA
Strand Antiparallel Antiparallel Antiparallel
Type of Helix Right-handed Right-handed Left-handed
Overall shape Long and
narrow
Short and wide Elongated and
narrow
Base pair per turn 10 11 12
Distance between adjacent
bases
0.34 nm 0.23 nm 0.38 nm
Pitch/turn of helix 3.40 nm 2.82 nm 4.56 nm
Helical Diameter 2.0 nm 2.3 nm 1.8 nm
Tilt/inclination of bp to axis 10 200 90
52. Property B-DNA A-DNA Z-DNA
MajorGroove Wide & Deep Narrow & Deep No discrenible
Minor Groove Narrow, shallow Broad, Shallow Narrow, Deep
53.
54.
55. • Two strands wind about each other
in a right-handed manner
• Diameter: ~20Å
• Bases per turn: 10 (~34Å)
• A major and a minor groove
20Å
Major
Minor
56. • One strand of DNA
goes from 5’ to 3’
(sugars)
• The other strand is
opposite in direction
going 3’ to 5’ (sugars)
57. • DNA is wrapped around
abundant nuclear
proteins called Histones
• This forms a complex
called a Nucleosome
• Histones are H1, H2A,
H2B, H3, H4