1
Dr. Manoranjan Mahakur
1st year MDS
Department of Pedodontics
&
Preventive Dentistry
2
DENTIN content at a glimpse……
 Interglobular Dentin
 Granular layer
 Sensation of dentin
 Porosity of dentin
 Changes of dentin
 History of Dentin !
 What is dentin ?
 Attitude of dentin
 Backbone of dentin
 HOEHL CELL
 Sibling of dentin
 METADENTIN
 Conglomerate line
3
HISTORY of DENTIN
the unrevealed…….
 1678- A.V Leeuwenhoek
 1771- John Hunter
“The Natural History of Human
Teeth”
“ Madder feeding experiment on pig ’’
1838- Sir john Tomes – Dentinal tubules
 Richard Owen- Dentin
(odontography-1840-45)
 Nasmyth- Ivory.
4
what is DENTIN ??
The fact
 Dentin (/ˈdɛntᵻn/)
(American English)
or dentine (/ˈdɛnˌtiːn/
or /ˌdɛnˈtiːn/) (British
English) (Latin:
substantia eburnea) is a
calcified tissue of the
body
 Dentine: ivory,
tooth bone,
zahnsubstanz,
substantia dentis
5
Dentin
Organic
(65-70)%
Collagen 82%
type 1
Type iii & v
Non collagenous
18%
(i)DSPP(DPP,DSP)
(ii)Enzyme
(v)Lipid
(iii)TGF,FGF,IGF-s,
BMP-s
In organic
(20-25)%
(i)Ca10(PO4)6
(OH)2
(ii)Salt of
(CO3,So4,Po4
(iii)Trace
elemernt
(Cu,fe,Zn.F)
H2O
(10-15)%
C
O
M
P
O
S
I
T
I
O
N
6
STRUCTURE …the backbone
7
Physical & Mechanical properties of DENTIN
the attitude……..
 THICKNESS-3-10 mm
 MODULOUS OF ELASTICITY-
15-20GPA
 HARDNESS-68KHN
CARIOUS DENTIN- 25KHN
SCLEROTIC DENTIN - 80KHN
 COMPRESSIVE STRENGTH- 266MPa
 TENSILE ETRENGTH-50 MPa
 RADIOOPACITY – 1
8
STRUCTURE of DENTIN
the architec …..
 Odontoblast process
 Dentinal tubules
 Non mineralized
matrix
( predentin)
• Minerlized matrix
( peritubular, inter
tubular, inter globular)
9
DENTINAL TUBULES(DT)
the pathway……
 Sigmoid shape
 Thickness 3-10 mm
 Outer surface dentin /inner
surface dentin= 5/1
 Diameter :pulpal > outer
(4 micro mt> 1 micro mt)
 Nos of tubules pulpal/outer
per unit are=4/1
 Lateral branches called
canaliculi or micro tubules
10
DENTINAL TUBULES(DT)
coronal ,middle ,apical
11
DENTINAL TUBULES(DT)
crown Vs root
12
Types of DENTIN…….
the sibling !
PRIMARY
DENTIN
SECONDARY
DENTIN
TERTIARY
DENTIN
13
PRIMARY DENTIN…..
Forms most of tooth
Laid down before completion of apical foramen
Contains straight tubules
Outlines pulp chamber = circumpulpal dentin
Outer layer = mantle dentin
14
SECONDARY DENTIN…..
Develops after completed root formation
Laid down as continuation of primary
Tubular structure (less regular than primary)
Change of tubules direction at the transition from
primary to secondary dentin
Slower rate of matrix deposition
Uneven deposition: more on roof and floor of pulp
chamber - leads to reduction of pulp space (PULP
RECESSION)
15
TERTIARY DENTIN…..
Produced in response to irritation or damage to the
overlying dentin and/or enamel
 by cells directly affected by stimulus
May be reactive or reparative dentin
Reactive dentin = produced by pre-existing Odontoblast
Reparative = produced by newly-differentiaed-
Odontoblast like cells
Irregularly arranged tubules
Fewer dentinal tubules or no tubules
with aging or severe damage, it can totally obliterate the
pulp cavity
16
Types of DENTIN cont….
17
PREDENTIN…..
the softest
Always adjacent
to pulp tissue
2-6 µm wide
Not mineralized
Collagen and
proteoglycans
secreted
Produced
Metadentin
18
MANTLE DENTIN…
the first form
 Below DEJ
 Cushioning ,
 20µm
 Von kroff’s fiber
 Type III
 Less mineralised
 Few curved and thin
tubules
 Unaffected in X linked
hypophosphatemia.
19
CIRCUMPULPAL DENTIN…
the main
Bulk of tooth
All dentin before
root compltion
More mineralized
Small dm collagen
4mm per day
Enlarged in rickets
20
PERITUBULAR DENTIN…..
the covering
 Dentin immediately surrounded
the dentinal tubules
 / Intratubular dentin
 Lamina limitans organic linning of
calcified tubules
 Periodontoblastic space-between
odontoblastic process and
peritubular dentin
 Dentinal fluid – flow out ward
 Higher k+ and lower Na+
 Missing in rodents incisor
 No collagen
Peritubal dentin
21
INTERTUBULAR DENTIN……
the middle man
Main body of
dentin
Located between
zone of
peritubular dentin
Type I collagen
3 layer model
Inter tubular
22
INTERGLOBULAR DENTIN…
 Mineralization of dentin
in small globules
 Fall to coalesce
 Zone of
hypomineralization
 Seen in crown
 Below mantel dentin
 Dentinal tubule pass
through it.
23
GRANULAR LAYER of DENTIN ……
tomes granular layer
 Due to coalescing &
looping of terminal
Tubules
 Seen in root, adjacent
to cementum
 More toward apex
 Unmineralized (like
interglobular dentin)
24
Interglobular dentin Vs Tomes granular layer
 Seen in root
 Calcium ++++
 Phosphorus +++
 Seen in crown
 Sulfur ++++
25
Hyaline layer of Hopewell-Smith
 Most peripheral layer of
initially
unmineralized dentin.
 It is 0.5-0.8 µm thick
and contains enamel
matrix-like protein as
well as the proteins of
dentin.
 Initial fibers of
the periodontal
ligament embed
 Aids cementum in
binding to the dentin.
CEMENTUM
HYALINE LAYER
TOMES GRANULAR LAYER
DENTIN
26
ODONTOBLAST…….
the founder
 First appear at 17-18 week
in utero
 Cell bodies Length 40µm
dm 7 µm
 Process DM at pulp 3-
4µm narrower to 1 µm).
 Matrix formation 10-4 µm
per day
 Secretion of type I
collagen,proteoglycans
 Enamel spindle
 Lateral branch
27
ODONTOBLAST…….
the founder
28
sequence of dentin formation…
 Coronal dentin formed
before root dentine.
 1st Cuspal then cervical
last apical.
 Dentin first is formed at
DEJ = the oldest dentin.
Pulpal-surface dentin
formed last = newest
dentin.
29
Incremental lines of DENTIN…..
the sequential
 Daily rhythmic deposition
of dentin
 Fine or striatation in
dentin
 Right angle to dentinal
tubules
(8 µm in crown3-4 µm in
root/day)
 Distance between
incremental line 4-8µm.
30
INCREMENTAL LINE OF DENTIN
VON
EBNER
OWEN
NEO
NATAL
•Daily
rhythmic
deposition of
dentin
•Accentuated
incremental line
due disturbance in
the matrix and
mineralization
•Prenatal and post
natal dentin are
separated by an
accentuated line
•Dentin form prior to
birth is better
31
Innervations of DENTIN
the sensation
 Intratubular nerve
 30-70% of odontoblastic
process
 Both myelinated and
unmylinated
 More near tip pulp horn
 In erupting teeth it
remain in pulp
 A & C fiber
 Subnucleus caudalis
32
Permeability of DENTIN…
 Highly permeable
 More towards pulp
 Transdentinal
 Intradentinal
 Thickness permeablity
 Tubular occlusion ,smear
layer permeablity
33
changes of DENTIN…
Attrition ,abrasion,erosion,cavity
preparation
Loss of Odontoblastic process
Dentinal tubules filled with gaseous
and liquid
Reparative
dentin seal
dentinal
tubules
DARK-
TRANSMITTED
LIGHT
WHITE –
REFLECTED LIGHT
34
changes of DENTIN…
Attrition ,abrasion,erosion,cavity
preparation
Collagen fibers & apatite crystal appear
Dentinal tubules filled with crystal
and obliterated
DARK
REFLECTED LIGHT
WHITE
TRANSMITTED LIGHT
35
changes of DENTIN…
Transmitted light
Reflected light
36
ANIMALS DENTIN…
the mysteries
 Ivory of elephant
 Herbivorous have
alternate ridge of enamel
and dentin
 Dermal denticle of
shark
 Osteodentin in rodents
& reparative dentin -
human
 Vasodentin- some fish
37
PREDENTIN
1- PRIMARY
2-SECONDARY
3- TERTIARY
1-MANTLE
2-CIRCUMPULPAL
1-PERITUBULAR
2- INTERTUBULAR
1- INTERGLOBULAR
2-TOMES GRANULAR
LAYER
DENTIN
ODONTOBLAST
HOEHLS
1-VON EBNER
2-OWEN
3-NEO NATAL
1-DEAD TRACT
2-SCLEROTIC
DENTIN
SUMMARY
38
summary……
39
Reference…….
 Orban’s Oral histology
and Embryology
 Ten cate’s oral histology
 Shafer’s Text book of
oral pathology
 Peritubular Dentin
Formation: Crystal
Organization and the
Macromolecular
Constituents in Human
Teeth☆
(JOURNAL OF STRUCTURAL
BIOLOGY)
 www.ncbi.nlm.nih.gov/pmc/articles/PMC3
360947
•Cohen, Stephen, and Kenneth M.
Hargreaves, eds. Pathways of the Pulp. 9th
ed. St. Louis, MO: Mosby Elsevier, 2006.
Print.
•Jump up^ Nanci, Antonio. Ten Cate's
Oral Histology: Development, Structure,
and Function. 7th ed.
• https://en.wikipedia.org/wiki/X-
linked_hypophosphatemia
40
“I will respect the hard-won scientific gains of those
physicians in whose steps I walk, and gladly share such
knowledge as is mine with those who are to follow”.
(Hippocratic Oath)
Upcoming Seminar ………..
Dentinogenesis &
Clinical consideration.

DENTIN (The facts and mysteries)

  • 1.
    1 Dr. Manoranjan Mahakur 1styear MDS Department of Pedodontics & Preventive Dentistry
  • 2.
    2 DENTIN content ata glimpse……  Interglobular Dentin  Granular layer  Sensation of dentin  Porosity of dentin  Changes of dentin  History of Dentin !  What is dentin ?  Attitude of dentin  Backbone of dentin  HOEHL CELL  Sibling of dentin  METADENTIN  Conglomerate line
  • 3.
    3 HISTORY of DENTIN theunrevealed…….  1678- A.V Leeuwenhoek  1771- John Hunter “The Natural History of Human Teeth” “ Madder feeding experiment on pig ’’ 1838- Sir john Tomes – Dentinal tubules  Richard Owen- Dentin (odontography-1840-45)  Nasmyth- Ivory.
  • 4.
    4 what is DENTIN?? The fact  Dentin (/ˈdɛntᵻn/) (American English) or dentine (/ˈdɛnˌtiːn/ or /ˌdɛnˈtiːn/) (British English) (Latin: substantia eburnea) is a calcified tissue of the body  Dentine: ivory, tooth bone, zahnsubstanz, substantia dentis
  • 5.
    5 Dentin Organic (65-70)% Collagen 82% type 1 Typeiii & v Non collagenous 18% (i)DSPP(DPP,DSP) (ii)Enzyme (v)Lipid (iii)TGF,FGF,IGF-s, BMP-s In organic (20-25)% (i)Ca10(PO4)6 (OH)2 (ii)Salt of (CO3,So4,Po4 (iii)Trace elemernt (Cu,fe,Zn.F) H2O (10-15)% C O M P O S I T I O N
  • 6.
  • 7.
    7 Physical & Mechanicalproperties of DENTIN the attitude……..  THICKNESS-3-10 mm  MODULOUS OF ELASTICITY- 15-20GPA  HARDNESS-68KHN CARIOUS DENTIN- 25KHN SCLEROTIC DENTIN - 80KHN  COMPRESSIVE STRENGTH- 266MPa  TENSILE ETRENGTH-50 MPa  RADIOOPACITY – 1
  • 8.
    8 STRUCTURE of DENTIN thearchitec …..  Odontoblast process  Dentinal tubules  Non mineralized matrix ( predentin) • Minerlized matrix ( peritubular, inter tubular, inter globular)
  • 9.
    9 DENTINAL TUBULES(DT) the pathway…… Sigmoid shape  Thickness 3-10 mm  Outer surface dentin /inner surface dentin= 5/1  Diameter :pulpal > outer (4 micro mt> 1 micro mt)  Nos of tubules pulpal/outer per unit are=4/1  Lateral branches called canaliculi or micro tubules
  • 10.
  • 11.
  • 12.
    12 Types of DENTIN……. thesibling ! PRIMARY DENTIN SECONDARY DENTIN TERTIARY DENTIN
  • 13.
    13 PRIMARY DENTIN….. Forms mostof tooth Laid down before completion of apical foramen Contains straight tubules Outlines pulp chamber = circumpulpal dentin Outer layer = mantle dentin
  • 14.
    14 SECONDARY DENTIN….. Develops aftercompleted root formation Laid down as continuation of primary Tubular structure (less regular than primary) Change of tubules direction at the transition from primary to secondary dentin Slower rate of matrix deposition Uneven deposition: more on roof and floor of pulp chamber - leads to reduction of pulp space (PULP RECESSION)
  • 15.
    15 TERTIARY DENTIN….. Produced inresponse to irritation or damage to the overlying dentin and/or enamel  by cells directly affected by stimulus May be reactive or reparative dentin Reactive dentin = produced by pre-existing Odontoblast Reparative = produced by newly-differentiaed- Odontoblast like cells Irregularly arranged tubules Fewer dentinal tubules or no tubules with aging or severe damage, it can totally obliterate the pulp cavity
  • 16.
  • 17.
    17 PREDENTIN….. the softest Always adjacent topulp tissue 2-6 µm wide Not mineralized Collagen and proteoglycans secreted Produced Metadentin
  • 18.
    18 MANTLE DENTIN… the firstform  Below DEJ  Cushioning ,  20µm  Von kroff’s fiber  Type III  Less mineralised  Few curved and thin tubules  Unaffected in X linked hypophosphatemia.
  • 19.
    19 CIRCUMPULPAL DENTIN… the main Bulkof tooth All dentin before root compltion More mineralized Small dm collagen 4mm per day Enlarged in rickets
  • 20.
    20 PERITUBULAR DENTIN….. the covering Dentin immediately surrounded the dentinal tubules  / Intratubular dentin  Lamina limitans organic linning of calcified tubules  Periodontoblastic space-between odontoblastic process and peritubular dentin  Dentinal fluid – flow out ward  Higher k+ and lower Na+  Missing in rodents incisor  No collagen Peritubal dentin
  • 21.
    21 INTERTUBULAR DENTIN…… the middleman Main body of dentin Located between zone of peritubular dentin Type I collagen 3 layer model Inter tubular
  • 22.
    22 INTERGLOBULAR DENTIN…  Mineralizationof dentin in small globules  Fall to coalesce  Zone of hypomineralization  Seen in crown  Below mantel dentin  Dentinal tubule pass through it.
  • 23.
    23 GRANULAR LAYER ofDENTIN …… tomes granular layer  Due to coalescing & looping of terminal Tubules  Seen in root, adjacent to cementum  More toward apex  Unmineralized (like interglobular dentin)
  • 24.
    24 Interglobular dentin VsTomes granular layer  Seen in root  Calcium ++++  Phosphorus +++  Seen in crown  Sulfur ++++
  • 25.
    25 Hyaline layer ofHopewell-Smith  Most peripheral layer of initially unmineralized dentin.  It is 0.5-0.8 µm thick and contains enamel matrix-like protein as well as the proteins of dentin.  Initial fibers of the periodontal ligament embed  Aids cementum in binding to the dentin. CEMENTUM HYALINE LAYER TOMES GRANULAR LAYER DENTIN
  • 26.
    26 ODONTOBLAST……. the founder  Firstappear at 17-18 week in utero  Cell bodies Length 40µm dm 7 µm  Process DM at pulp 3- 4µm narrower to 1 µm).  Matrix formation 10-4 µm per day  Secretion of type I collagen,proteoglycans  Enamel spindle  Lateral branch
  • 27.
  • 28.
    28 sequence of dentinformation…  Coronal dentin formed before root dentine.  1st Cuspal then cervical last apical.  Dentin first is formed at DEJ = the oldest dentin. Pulpal-surface dentin formed last = newest dentin.
  • 29.
    29 Incremental lines ofDENTIN….. the sequential  Daily rhythmic deposition of dentin  Fine or striatation in dentin  Right angle to dentinal tubules (8 µm in crown3-4 µm in root/day)  Distance between incremental line 4-8µm.
  • 30.
    30 INCREMENTAL LINE OFDENTIN VON EBNER OWEN NEO NATAL •Daily rhythmic deposition of dentin •Accentuated incremental line due disturbance in the matrix and mineralization •Prenatal and post natal dentin are separated by an accentuated line •Dentin form prior to birth is better
  • 31.
    31 Innervations of DENTIN thesensation  Intratubular nerve  30-70% of odontoblastic process  Both myelinated and unmylinated  More near tip pulp horn  In erupting teeth it remain in pulp  A & C fiber  Subnucleus caudalis
  • 32.
    32 Permeability of DENTIN… Highly permeable  More towards pulp  Transdentinal  Intradentinal  Thickness permeablity  Tubular occlusion ,smear layer permeablity
  • 33.
    33 changes of DENTIN… Attrition,abrasion,erosion,cavity preparation Loss of Odontoblastic process Dentinal tubules filled with gaseous and liquid Reparative dentin seal dentinal tubules DARK- TRANSMITTED LIGHT WHITE – REFLECTED LIGHT
  • 34.
    34 changes of DENTIN… Attrition,abrasion,erosion,cavity preparation Collagen fibers & apatite crystal appear Dentinal tubules filled with crystal and obliterated DARK REFLECTED LIGHT WHITE TRANSMITTED LIGHT
  • 35.
  • 36.
    36 ANIMALS DENTIN… the mysteries Ivory of elephant  Herbivorous have alternate ridge of enamel and dentin  Dermal denticle of shark  Osteodentin in rodents & reparative dentin - human  Vasodentin- some fish
  • 37.
    37 PREDENTIN 1- PRIMARY 2-SECONDARY 3- TERTIARY 1-MANTLE 2-CIRCUMPULPAL 1-PERITUBULAR 2-INTERTUBULAR 1- INTERGLOBULAR 2-TOMES GRANULAR LAYER DENTIN ODONTOBLAST HOEHLS 1-VON EBNER 2-OWEN 3-NEO NATAL 1-DEAD TRACT 2-SCLEROTIC DENTIN SUMMARY
  • 38.
  • 39.
    39 Reference…….  Orban’s Oralhistology and Embryology  Ten cate’s oral histology  Shafer’s Text book of oral pathology  Peritubular Dentin Formation: Crystal Organization and the Macromolecular Constituents in Human Teeth☆ (JOURNAL OF STRUCTURAL BIOLOGY)  www.ncbi.nlm.nih.gov/pmc/articles/PMC3 360947 •Cohen, Stephen, and Kenneth M. Hargreaves, eds. Pathways of the Pulp. 9th ed. St. Louis, MO: Mosby Elsevier, 2006. Print. •Jump up^ Nanci, Antonio. Ten Cate's Oral Histology: Development, Structure, and Function. 7th ed. • https://en.wikipedia.org/wiki/X- linked_hypophosphatemia
  • 40.
    40 “I will respectthe hard-won scientific gains of those physicians in whose steps I walk, and gladly share such knowledge as is mine with those who are to follow”. (Hippocratic Oath) Upcoming Seminar ……….. Dentinogenesis & Clinical consideration.