Culture of normally sterile fluids
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The document discusses culturing procedures for normally sterile fluids and sites, including cerebrospinal fluid, pericardial effusions, bone marrow, and other effusions. Cerebrospinal fluid should be collected aseptically and inoculated into transport media if not being transported immediately to the laboratory for processing. Pericardial effusions can be caused by a variety of bacteria. Bone marrow samples should be inoculated into nutrient broth. All sterile fluids require aseptic collection and microscopic examination and culturing to identify potential pathogenic bacteria.
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3. Cytology of body fluids provides diagnostic information for conditions affecting various organ systems. Proper collection and analysis of physical and chemical properties aids in differential diagnosis.
Culture media
Bacteria have various nutritional requirements including water, carbon and nitrogen sources, inorganic salts, vitamins, and certain gaseous and temperature conditions to grow. Different types of culture media can be used for bacterial cultivation based on ingredients, agar concentration, and special properties. These include basic, complex, synthetic, enriched, selective, differential, and transport media formulated for specific bacterial isolation and identification purposes.
Culture methods
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2. Common culture methods include streak culture, lawn culture, stroke culture, stab culture, pour plate culture, and liquid culture.
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Staphylococcus is a common cause of skin infections in humans. Staphylococcus aureus is an important pathogenic species. It is gram-positive, catalase-positive, and produces coagulase. S. aureus causes a variety of infections, including skin and soft tissue infections like boils and abscesses. It can also cause pneumonia, osteomyelitis, toxic shock syndrome, and food poisoning. Laboratory diagnosis involves culturing specimens on blood agar and performing tests like the coagulase test and mannitol fermentation. Treatment involves antibiotics like penicillin, cloxacillin, or vancomycin for resistant strains.
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