Bacteriological Investigation of Well Water Samples from Selected Market Loca...inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
An Assessment on Drinking Water Quality and Management in Kakamega Municipalitypaperpublications3
Abstract: Drinking water must be free from components which may adversely affect the human health. Such components include minerals, organic substances and disease causing microorganisms. A large portion of the population in urban areas in developing countries suffers from health problems associated with either lack of drinking water or due to the presence of microbiological contamination in water. This research was conducted in Kakamega municipality with a broad objective to conduct assessment of water quality and management in Kakamega municipality. The Specific objective was to determine the chemical water quality parameters in water and to evaluate the management practices on water in Kakamega municipality. Four water quality parameters; two physical and two chemical were tested from the samples collected for this research work. Sampling technique was purposive where water samples from water sources and distribution points in densely populated areas of Kakamega municipality were taken. Data collection instruments that were used included sterilized bottles to collect water, delivery to the laboratory within six (6) hours of collection for reliable results and data quality control was achieved through immediate entry in the pre-designed data form. According to the results pH values at all the sources and house connections are well within the WHO desirable limit of 6.50-8.0. The sample from Sichirai had a pH of 7.8 that was the highest as compared to an Isiukhu river that had 6.6 pH. The samples from Isiukhu river, Savona Island River, fishpond at bridge and Shikhambi spring showed more than 5 NTU. The researchers recommended for water surveillance in Kakamega municipality in order to ensure consumers have safe water free from agricultural and industrial chemical pollution.
Physico-Chemical and Microbial Analysis of Drinking Water of Four Springs of ...IJEAB
Drinking water of good quality is essential for human physiology whose continual existence depends on the availability of water and any sort of contamination in water which is above the standard limits set by international water regulating agencies can lead to water related diseases. So, the present investigation was conducted to determine the physico-chemical and bacteriological contents of four springs i.e.Heshi spring 1, Heshi spring 2, Kitaab Roong, and Kooti spring and its distribution system such as water reservoir inlet, outlet, mid and end point of distribution systems, junction where it merge with glacier water. The temperature was in a range of 13oC - 22oC. The turbidity of water samples fluctuate from 0.02NTU-1.99NTU. The pH value was in a range of 6.2-7.1. Electrical conductivity range of minimum 122µS/cm to a maximum of 600µS/cm. The TDS of all water samples ranging from minimum of 164-513mg/l. The amount of reactive ortho phosphate was in a range of 26mg/l to 59mg/L. The amount of total phosphorous was in a range of minimum 23m/L to maximum of 120mg/L. The total bacterial count was in a range of 11CFU/100ml to 83 CFU/100ml.The findings showed there should be comprehensive standardization of drinking water of Danyore village according to guidelines of WHO water quality standards and make it safe for human consumption.
Detection Of Escherichia Coli, An Indicator Of Feacal Contamination, In Drink...IOSR Journals
The detection of Escherichia coli as an indicator of faucal contamination in drinking water sources in Amassoma town, a host Community of the Niger Delta University, Bayelsa State in the Niger Delta of Nigeria, was carried out to determine their suitability for drinking. Result obtained showed mean total coliform bacterial counts of 2.05 x103 cfu/ml for borehole water, 1.25x103 cfu/ml for well water and 1.0x103 for pipe borne water. The mean count of faecal coliform was 2.1x103 cfu/ml for borehole water, 4.5x10 cfu/ml for well water and 1.0x10 cfu/ml for pipe borne water. The faecal coliform identified was Escherichia coli. Sources of contamination were found to be septic tanks, waste dump sites and periodic flooding of the area, being a typical wetland environment. It was concluded that water from the different sources studied in Amassoma did not meet the world health Organization (WHO) standard for drinking water. This study has therefore shown the need for continuous monitoring of our water supply systems.
Bacteriological Investigation of Well Water Samples from Selected Market Loca...inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
An Assessment on Drinking Water Quality and Management in Kakamega Municipalitypaperpublications3
Abstract: Drinking water must be free from components which may adversely affect the human health. Such components include minerals, organic substances and disease causing microorganisms. A large portion of the population in urban areas in developing countries suffers from health problems associated with either lack of drinking water or due to the presence of microbiological contamination in water. This research was conducted in Kakamega municipality with a broad objective to conduct assessment of water quality and management in Kakamega municipality. The Specific objective was to determine the chemical water quality parameters in water and to evaluate the management practices on water in Kakamega municipality. Four water quality parameters; two physical and two chemical were tested from the samples collected for this research work. Sampling technique was purposive where water samples from water sources and distribution points in densely populated areas of Kakamega municipality were taken. Data collection instruments that were used included sterilized bottles to collect water, delivery to the laboratory within six (6) hours of collection for reliable results and data quality control was achieved through immediate entry in the pre-designed data form. According to the results pH values at all the sources and house connections are well within the WHO desirable limit of 6.50-8.0. The sample from Sichirai had a pH of 7.8 that was the highest as compared to an Isiukhu river that had 6.6 pH. The samples from Isiukhu river, Savona Island River, fishpond at bridge and Shikhambi spring showed more than 5 NTU. The researchers recommended for water surveillance in Kakamega municipality in order to ensure consumers have safe water free from agricultural and industrial chemical pollution.
Physico-Chemical and Microbial Analysis of Drinking Water of Four Springs of ...IJEAB
Drinking water of good quality is essential for human physiology whose continual existence depends on the availability of water and any sort of contamination in water which is above the standard limits set by international water regulating agencies can lead to water related diseases. So, the present investigation was conducted to determine the physico-chemical and bacteriological contents of four springs i.e.Heshi spring 1, Heshi spring 2, Kitaab Roong, and Kooti spring and its distribution system such as water reservoir inlet, outlet, mid and end point of distribution systems, junction where it merge with glacier water. The temperature was in a range of 13oC - 22oC. The turbidity of water samples fluctuate from 0.02NTU-1.99NTU. The pH value was in a range of 6.2-7.1. Electrical conductivity range of minimum 122µS/cm to a maximum of 600µS/cm. The TDS of all water samples ranging from minimum of 164-513mg/l. The amount of reactive ortho phosphate was in a range of 26mg/l to 59mg/L. The amount of total phosphorous was in a range of minimum 23m/L to maximum of 120mg/L. The total bacterial count was in a range of 11CFU/100ml to 83 CFU/100ml.The findings showed there should be comprehensive standardization of drinking water of Danyore village according to guidelines of WHO water quality standards and make it safe for human consumption.
Detection Of Escherichia Coli, An Indicator Of Feacal Contamination, In Drink...IOSR Journals
The detection of Escherichia coli as an indicator of faucal contamination in drinking water sources in Amassoma town, a host Community of the Niger Delta University, Bayelsa State in the Niger Delta of Nigeria, was carried out to determine their suitability for drinking. Result obtained showed mean total coliform bacterial counts of 2.05 x103 cfu/ml for borehole water, 1.25x103 cfu/ml for well water and 1.0x103 for pipe borne water. The mean count of faecal coliform was 2.1x103 cfu/ml for borehole water, 4.5x10 cfu/ml for well water and 1.0x10 cfu/ml for pipe borne water. The faecal coliform identified was Escherichia coli. Sources of contamination were found to be septic tanks, waste dump sites and periodic flooding of the area, being a typical wetland environment. It was concluded that water from the different sources studied in Amassoma did not meet the world health Organization (WHO) standard for drinking water. This study has therefore shown the need for continuous monitoring of our water supply systems.
ANALYSIS OF RISK FACTORS ASSOCIATED WITH TRANSFERS OF CRYPTOSPORIDIUM
OOCYSTS IN AQUATIC ECOSYSTEMS OF HAITI
BALTHAZARD-ACCOU, Ketty1, PATRICE, Agnamey2, CLERVIL, Elmyre1, and EMMANUEL,
Evens1, (1) Universite Quisqueya, 218, Avenue Jean Paul II, Haut de Turgeau, Port-au-
Prince, HT6114, Haiti, kettybal@gmail.com, (2) Universite Picardie Jules Verne, Faculté de
médecine et CHU d’Amiens, Amiens, 80054, France
Cryptosporidium are protozoan parasites that infect the intestines of many vertebrates,
including humans. They cause cryptosporidiosis; one of the main traits of chronic diarrhea in
immunocompromised patients infected with HIV and acute diarrhea in young children. In Haiti,
the intestinal cryptosporidiosis is caused by at least three species: Cryptosporidium hominis,
C. parvum and C. felis wich are responsible for 17% of acute diarrhea in children less than 2
years and 30% of chronic diarrhea in patients infected with HIV. Transmission of Cryptosporidium
oocysts is often caused by the ingestion of contaminated food or water. In previous studies,
Cryptosporidium oocysts have been detected in surface water and groundwater from three
major cities of Haiti: Port-au-Prince, Cap-Haitian and Les Cayes. Significant concentrations
from 4 to 1274 oocysts in 100 liters of filtered water were determined in Port-au-Prince; in the
city of Cap-Haitian 741 to 6088 and concentrations ranging from 5 to 100 oocysts have been
obtained in groundwater from Les Cayes. Results indicate that water resources in Haiti are widely
contaminated with Cryptosporidium oocysts. Therefore, they may constitute potential sources of
biological risk particularly for human health. Since Cryptosporidium oocysts are biological colloids,
it is important to analyze the different risk factors associated with transfers of Cryptosporidium
oocysts in aquatic ecosystems of Haiti.
Microbiological quality of drinking water Mirko Rossi
Lecture on microbiological quality methods for drinking water for the faculty of Veterinary Medicine, University of Helsinki; Course in Environmental Health
Investigation of Toxic Substance in Basra WatersIJCMESJOURNAL
There was a need to investigate the presence of toxic elements and compounds in drinking and desalination water for the population of the Basra city, especially after increasing cases of poisoning and diarrhea recently to more than 70,000 cases in the province of Basra according to the Human Rights Commission in the province . Six Samples (A, B, C, D, E, F) of drinking water and human uses of (RO) waters packaged in bottles produced by a private company (sample A), (RO) waters for direct sale (sample B) , waters desalination for the areas of Qibla, Jubaila and Brathaia (samples C,D,E) in addition to the waters of Shatt Al-Arab (sample F) for the purpose of conducting analysis and knowing the percentages of toxic elements and compounds in the compositions of these samples. The results of the analysis of the samples under the study confirmed that the ratio of total soluble solids (TDS) of the samples ( D, E , F ) to the other samples (A, B, C) ). The results of the analysis also confirmed the high percentage of the lead element (Pb) of the two samples (E, F) from the other samples. The results showed an increase in sulphate (SO4) for sample (E) than the other samples. The results showed an increase in the ratio of phosphate (PO4) of sample (F) to the rest of the samples in comparison with the standards of the World Health Organization (WHO). These results indicate that pollution in the Shatt Al-Arab waters increased with toxic elements and compounds. These results correspond to the statistics of the Directorate of Basra Heaith, where it showed the increase in the proportion of casualties among the population of the province of Basra to areas adjacent to the Shatt Al-Arab river, such as the city center , Abu- Khasseb and Al-Tanuma from the rest of the areas causing water pollution and the spread of E. coli causing diarrhea and intestinal colic with 50% of cases which was admitted to Basra hospitals.
ANALYSIS OF RISK FACTORS ASSOCIATED WITH TRANSFERS OF CRYPTOSPORIDIUM
OOCYSTS IN AQUATIC ECOSYSTEMS OF HAITI
BALTHAZARD-ACCOU, Ketty1, PATRICE, Agnamey2, CLERVIL, Elmyre1, and EMMANUEL,
Evens1, (1) Universite Quisqueya, 218, Avenue Jean Paul II, Haut de Turgeau, Port-au-
Prince, HT6114, Haiti, kettybal@gmail.com, (2) Universite Picardie Jules Verne, Faculté de
médecine et CHU d’Amiens, Amiens, 80054, France
Cryptosporidium are protozoan parasites that infect the intestines of many vertebrates,
including humans. They cause cryptosporidiosis; one of the main traits of chronic diarrhea in
immunocompromised patients infected with HIV and acute diarrhea in young children. In Haiti,
the intestinal cryptosporidiosis is caused by at least three species: Cryptosporidium hominis,
C. parvum and C. felis wich are responsible for 17% of acute diarrhea in children less than 2
years and 30% of chronic diarrhea in patients infected with HIV. Transmission of Cryptosporidium
oocysts is often caused by the ingestion of contaminated food or water. In previous studies,
Cryptosporidium oocysts have been detected in surface water and groundwater from three
major cities of Haiti: Port-au-Prince, Cap-Haitian and Les Cayes. Significant concentrations
from 4 to 1274 oocysts in 100 liters of filtered water were determined in Port-au-Prince; in the
city of Cap-Haitian 741 to 6088 and concentrations ranging from 5 to 100 oocysts have been
obtained in groundwater from Les Cayes. Results indicate that water resources in Haiti are widely
contaminated with Cryptosporidium oocysts. Therefore, they may constitute potential sources of
biological risk particularly for human health. Since Cryptosporidium oocysts are biological colloids,
it is important to analyze the different risk factors associated with transfers of Cryptosporidium
oocysts in aquatic ecosystems of Haiti.
Microbiological quality of drinking water Mirko Rossi
Lecture on microbiological quality methods for drinking water for the faculty of Veterinary Medicine, University of Helsinki; Course in Environmental Health
Investigation of Toxic Substance in Basra WatersIJCMESJOURNAL
There was a need to investigate the presence of toxic elements and compounds in drinking and desalination water for the population of the Basra city, especially after increasing cases of poisoning and diarrhea recently to more than 70,000 cases in the province of Basra according to the Human Rights Commission in the province . Six Samples (A, B, C, D, E, F) of drinking water and human uses of (RO) waters packaged in bottles produced by a private company (sample A), (RO) waters for direct sale (sample B) , waters desalination for the areas of Qibla, Jubaila and Brathaia (samples C,D,E) in addition to the waters of Shatt Al-Arab (sample F) for the purpose of conducting analysis and knowing the percentages of toxic elements and compounds in the compositions of these samples. The results of the analysis of the samples under the study confirmed that the ratio of total soluble solids (TDS) of the samples ( D, E , F ) to the other samples (A, B, C) ). The results of the analysis also confirmed the high percentage of the lead element (Pb) of the two samples (E, F) from the other samples. The results showed an increase in sulphate (SO4) for sample (E) than the other samples. The results showed an increase in the ratio of phosphate (PO4) of sample (F) to the rest of the samples in comparison with the standards of the World Health Organization (WHO). These results indicate that pollution in the Shatt Al-Arab waters increased with toxic elements and compounds. These results correspond to the statistics of the Directorate of Basra Heaith, where it showed the increase in the proportion of casualties among the population of the province of Basra to areas adjacent to the Shatt Al-Arab river, such as the city center , Abu- Khasseb and Al-Tanuma from the rest of the areas causing water pollution and the spread of E. coli causing diarrhea and intestinal colic with 50% of cases which was admitted to Basra hospitals.
The Fab Lab Life Cycle; Report of the FAB10 workshops; Pieter van der Hijden ...Pieter van der Hijden
The Fab Lab Life Cycle; Report of the FAB10 Workshops; Pieter van der Hijden* & Beno Juarez** with help from Enrico Bassi, Klaas Hernamdt, Massimo Menichinelli, Dirk van Vreeswijk, Anna Waldman-Brown
* Fab Lab Paramaribo (Suriname) & Sofos Consultancy (Amsterdam, The Netherlands), ** Fab Lab Lima (Peru)
FAB10 - International Fab Lab Conference, Barcelona, Spain, 2-8 July 2014
Semiconductor Fab Trends and Forecast - June 2013SEMI
The presentation contains Information and data on frontend semiconductor and LED fabs
fabs. The charts and tables are from an article published on May 31, 2013. Visit http://www.semi.org/en/node/45921 to read the entire article.
Other resources:
www.semi.org/fabs
www.semi.org/marketinfo
Bacteria of tomatoes managed with well water and pond water: Impact of agricu...Agriculture Journal IJOEAR
Abstract— We know that contamination of crops by human pathogens can occur in agricultural settings but we still do not understand precisely which environmental sources represent the highest risks. Human pathogens maybe introduced by wind, worker hygiene, plant mediated factors, insects, water sources, or any combination of these factors. To safeguard against risks to consumers from agricultural waters, FSMA regulations for U.S. crop production require the use of water with an average of less than 126 CFU per 100 ml for applications that come in direct contact with a crop. Due to availability/scarcity however, water from other sources such as agricultural ponds is commonly used. To better understand risks that may be associated with the use of “surface”(often higher microbial load) water sources, we described the bacterial microbiota associated with an agricultural pond, an agricultural well and the corresponding microbiota of tomato carpospheres managed with each water source (also described as phyllosphere). 16S rRNA gene amplicons were used for bacterial profiling of waters and tomato surfaces at four time points over a growing season. Microbial profiles differentiated surface and ground water samples throughout the season, however no significant influence on tomato fruit surfaces could be correlated to either water source. These results suggest that in certain cases, environmental pressures such as wind, dust or other airborne factors may have a more significant impact on the surface microbiology of field crops than irrigation or management water sources do.
The objective of the study was determining the pot ability of water from some part of Visnupuri area Nanded. The total coliform count in drinking water samples was in the ranges of 140-920 MPN index/100 ml. The data suggested that the quality of drinking water deterioration in rural habitations of this region was due to poor sanitation & contaminated water supply. The occurrence of some pathogenic bacteria in drinking water may increase the risk of water related diseases & health
problem in local residents.
water, a substance composed of the chemical elements hydrogen and oxygen and existing in gaseous, liquid, and solid states. It is one of the most plentiful and essential of compounds. A tasteless and odourless liquid at room temperature, it has the important ability to dissolve many other substances.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
Global launch of the Healthy Ageing and Prevention Index 2nd wave – alongside...ILC- UK
The Healthy Ageing and Prevention Index is an online tool created by ILC that ranks countries on six metrics including, life span, health span, work span, income, environmental performance, and happiness. The Index helps us understand how well countries have adapted to longevity and inform decision makers on what must be done to maximise the economic benefits that comes with living well for longer.
Alongside the 77th World Health Assembly in Geneva on 28 May 2024, we launched the second version of our Index, allowing us to track progress and give new insights into what needs to be done to keep populations healthier for longer.
The speakers included:
Professor Orazio Schillaci, Minister of Health, Italy
Dr Hans Groth, Chairman of the Board, World Demographic & Ageing Forum
Professor Ilona Kickbusch, Founder and Chair, Global Health Centre, Geneva Graduate Institute and co-chair, World Health Summit Council
Dr Natasha Azzopardi Muscat, Director, Country Health Policies and Systems Division, World Health Organisation EURO
Dr Marta Lomazzi, Executive Manager, World Federation of Public Health Associations
Dr Shyam Bishen, Head, Centre for Health and Healthcare and Member of the Executive Committee, World Economic Forum
Dr Karin Tegmark Wisell, Director General, Public Health Agency of Sweden
Telehealth Psychology Building Trust with Clients.pptxThe Harvest Clinic
Telehealth psychology is a digital approach that offers psychological services and mental health care to clients remotely, using technologies like video conferencing, phone calls, text messaging, and mobile apps for communication.
The Importance of Community Nursing Care.pdfAD Healthcare
NDIS and Community 24/7 Nursing Care is a specific type of support that may be provided under the NDIS for individuals with complex medical needs who require ongoing nursing care in a community setting, such as their home or a supported accommodation facility.
How many patients does case series should have In comparison to case reports.pdfpubrica101
Pubrica’s team of researchers and writers create scientific and medical research articles, which may be important resources for authors and practitioners. Pubrica medical writers assist you in creating and revising the introduction by alerting the reader to gaps in the chosen study subject. Our professionals understand the order in which the hypothesis topic is followed by the broad subject, the issue, and the backdrop.
https://pubrica.com/academy/case-study-or-series/how-many-patients-does-case-series-should-have-in-comparison-to-case-reports/
Explore our infographic on 'Essential Metrics for Palliative Care Management' which highlights key performance indicators crucial for enhancing the quality and efficiency of palliative care services.
This visual guide breaks down important metrics across four categories: Patient-Centered Metrics, Care Efficiency Metrics, Quality of Life Metrics, and Staff Metrics. Each section is designed to help healthcare professionals monitor and improve care delivery for patients facing serious illnesses. Understand how to implement these metrics in your palliative care practices for better outcomes and higher satisfaction levels.
Navigating Challenges: Mental Health, Legislation, and the Prison System in B...Guillermo Rivera
This conference will delve into the intricate intersections between mental health, legal frameworks, and the prison system in Bolivia. It aims to provide a comprehensive overview of the current challenges faced by mental health professionals working within the legislative and correctional landscapes. Topics of discussion will include the prevalence and impact of mental health issues among the incarcerated population, the effectiveness of existing mental health policies and legislation, and potential reforms to enhance the mental health support system within prisons.
Antibiotic Stewardship by Anushri Srivastava.pptxAnushriSrivastav
Stewardship is the act of taking good care of something.
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
WHO launched the Global Antimicrobial Resistance and Use Surveillance System (GLASS) in 2015 to fill knowledge gaps and inform strategies at all levels.
ACCORDING TO apic.org,
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
ACCORDING TO pewtrusts.org,
Antibiotic stewardship refers to efforts in doctors’ offices, hospitals, long term care facilities, and other health care settings to ensure that antibiotics are used only when necessary and appropriate
According to WHO,
Antimicrobial stewardship is a systematic approach to educate and support health care professionals to follow evidence-based guidelines for prescribing and administering antimicrobials
In 1996, John McGowan and Dale Gerding first applied the term antimicrobial stewardship, where they suggested a causal association between antimicrobial agent use and resistance. They also focused on the urgency of large-scale controlled trials of antimicrobial-use regulation employing sophisticated epidemiologic methods, molecular typing, and precise resistance mechanism analysis.
Antimicrobial Stewardship(AMS) refers to the optimal selection, dosing, and duration of antimicrobial treatment resulting in the best clinical outcome with minimal side effects to the patients and minimal impact on subsequent resistance.
According to the 2019 report, in the US, more than 2.8 million antibiotic-resistant infections occur each year, and more than 35000 people die. In addition to this, it also mentioned that 223,900 cases of Clostridoides difficile occurred in 2017, of which 12800 people died. The report did not include viruses or parasites
VISION
Being proactive
Supporting optimal animal and human health
Exploring ways to reduce overall use of antimicrobials
Using the drugs that prevent and treat disease by killing microscopic organisms in a responsible way
GOAL
to prevent the generation and spread of antimicrobial resistance (AMR). Doing so will preserve the effectiveness of these drugs in animals and humans for years to come.
being to preserve human and animal health and the effectiveness of antimicrobial medications.
to implement a multidisciplinary approach in assembling a stewardship team to include an infectious disease physician, a clinical pharmacist with infectious diseases training, infection preventionist, and a close collaboration with the staff in the clinical microbiology laboratory
to prevent antimicrobial overuse, misuse and abuse.
to minimize the developme
Leading the Way in Nephrology: Dr. David Greene's Work with Stem Cells for Ki...Dr. David Greene Arizona
As we watch Dr. Greene's continued efforts and research in Arizona, it's clear that stem cell therapy holds a promising key to unlocking new doors in the treatment of kidney disease. With each study and trial, we step closer to a world where kidney disease is no longer a life sentence but a treatable condition, thanks to pioneers like Dr. David Greene.
3. Intracellular parasite that infect vertebrates including
humans.
Belongs to Apicomplexa an obligate parasitic group of
Eukaryotes.
Species of cryptospordium parasitize specific host
tissues such as stomach, small intestine or trachea.
Cryptosporidiosis is a self limiting disease in healthy host but
represents a life threatening problem in immunocompromised
individuals for which there is no effective method.
4. The life cycle of this
parasite is complex,
showing both asexual
and sexual stages.
The persistence of
oocysts in environmental
compartment can be
influenced by their age
and chemical
disinfectants.
Under natural conditions,
fecal matter shelters
oocysts from desiccation
and increase
impermeability of the wall
to small molecules, thus
reducing their exposure
to lethal environmental
factors.
The resistance of oocysts
in a solid matrix such as
soil has become a crucial
parameter in
understanding their
transfer to lower layers.
5. Oocyst can remain viable and infectious in
water for several months at temperatures
ranging from 0 to 30c.
Boiling water can kill cryptosporidium
oocysts in less than a minute.
Exposure to sunlight had no effect on the
viability of cryptosporidium oocysts but UV
at 265nm & black light at 365nm lead to a
reduction in the no. of viable oocysts.
Oocysts resistance
6. Filtration
Methods
Membrane
Filtration
Cartridge
Filtration
Woven Acrylic Polyethersulfone
Chemical
Flocculation
Another method is elution &
its efficiency depends on
turbidity of sample, the type
of filter & the method
chosen. Oocysts trapped by
filter are eluted by detergent
solutions that break
hydrophobic interactions
b/w oocysts, sediment
particles and filter fibers.
Now centrifuge the eluate to
improve separation of
desired oocysts. Use
solutions containing
dispersive agents i.e, EDTA
increases the average % of
cryptosporidium oocysts
recovery.
7. NRC 1983 defines risk evaluation of toxic
characteristics of a chemical product and
conditions of human exposure to this
product.
Health risk evaluation relies on an
epidemiological approach.
Evaluating health risk is based on four steps:
Identifying the danger
Dose response relationship
Estimating exposure
Characterizing the risk
8.
9. Water distribution were analyzed at different
periods by sampling at least 100L.
Samples were taken from 8 reservoirs, 7
points of city drinking water network and 3
public fountains.
The exposed population was divided into 4
categories:
1
• Immunocompetent aged 5 & over low
risk of infection.
2
• Immunodepressed aged 5 & over with
severe illness linked to HIV.
3
• Immunocompetent under 5 years old
high risk of illness.
4
• Immunodepressed under 5 years old
leads to high risk of illness.
Number of persons
contaminated by HIV in
Haiti is estimated to be
about 400,000 i.e, 5% of
the total population.
Following equation was used to determine the
probability of infection:
Pr = 1- exp(-rD)
Where:
P= probability of infection of an individual exposed
to Dose D of oocysts
r= the probability of survival of oocysts in drinking
water ingested by the host
D= ingested Dose
10. Highlighted the presence of cryptosporidium
oocysts in the surface and ground water of
the coastal city of Cayes.
The watershed is divided by 3 distinct types
of Aquifers: Alluvial unconfined underground,
karastic and cracked and segmented
carbonate.
Water samples were taken from 15 sites in
September, November and December 2007,
i.e at the end of rainy season and at the
beginning of the main dry season.
6 sites were found
to be positive for
cryptosporidium
oocysyts per 100L
and elution method
used .
9 oocysts/ 100L
were obtained from
water samples from
public water supply
service of Cayes.
11. A risk factor is an
element belonging to an
individual or originating
from an environment
liable to cause an
illness, trauma or
another negative impact
on the development of
human organism.
The exposure of
consumer to
contaminated water is a
major health risk factor.
There is no control for
quality of the water of
the water distributed by
public services.
Urban areas lack basic
services for the
collection and treatment
of wastewater and solid
wastes and the removal
of excreta.
Chlorination is the only
method used to treat
raw water but it is
ineffective in
inactivating oocysts.
12. Conclusion:
Risk assessment is not a goal but there is an
decision making for risk management of oocyst
hazards and risks for human health.