This document discusses different types of connective tissue cells. There are two main types of connective tissue cells: fixed and free cells. Free cells include plasma cells, mast cells, leukocytes, and pigment cells. Plasma cells are large spherical cells that produce immunoglobulins and antibodies. Mast cells are ovoid cells found in connective tissue that contain basophilic granules and play roles in allergic reactions and inflammation. Pigment cells contain pigments and are found in various tissues. Leukocytes, or white blood cells, include granulocytes like neutrophils, basophils and eosinophils, as well as agranulocytes like lymphocytes and monocytes.

1. 1

2. CONNECTIVE TISSUE
Connective Tissue Cells a- Fixed ,
b- Free
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3. CONNECTIVE TISSUE
Free CT cells
 Plasma cells
 Mast cells
 Leucocytes
 Pigment cells
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4. CONNECTIVE TISSUE
Plasma cells
LM:
Shape : Large spherical cell (20 µm)
Nucleus : spherical and eccentric nucleus
Cytoplasm: Basophilic in H&E stain.
It shows a pale area close to the nucleus with a well developed Golgi
called (Negative Golgi Image), spherical inclusions called (Russell
Bodies) . 4Dr. M.Attia

5. CONNECTIVE TISSUE
Plasma cells
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6. CONNECTIVE TISSUE
EM:
 The nucleus has large clumbs of heterochromatin alternating with
light area of euchromatin giving the clock face appearance.
 The cytoplasm contains mitochondria, rough endoplasmic reticulum,
Golgi complex, many free ribosomes.
 Function:
Immunoglobins production ( Antibodies)
Plasma cells
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7. CONNECTIVE TISSUE
Mast cell
Pluripotent stem cell in the bone marrow
It has specific surface receptors for the Ig E
antibodies produced by plasma cells.
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8. CONNECTIVE TISSUE
Mast cell
LM:
Shape : Large ovoid cell (20 µm)
Nucleus : spherical and central nucleus
Cytoplasm: filled with large basophilic granules in
H&E stain , shows metachromasia.
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9. CONNECTIVE TISSUE
EM:
 The cytoplasm contains mitochondria, rough endoplasmic
reticulum, Golgi complex
 Granules appear as electron dense spherical membranous
bounded.
Mast cell
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10. CONNECTIVE TISSUE
The mast cell granules contain :
A- Primary mediators: heparine, histamine, serotonin, eosinophil,
chemotactic factor.
B- Secondary mediators : substance of anaphylaxix and
prostaglandins.
Mast cell
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11. CONNECTIVE TISSUE
Mast cell
Functions
1- Allergic reaction and inflammation
2- Anticoagulant reaction
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12. CONNECTIVE TISSUE
Pigment cell
Cells containing pigments are found in:
Choroid of the eye
Pia mater
Dense CT of the skin.
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13. Leucocytes (WBCs)
CONNECTIVE TISSUE
They are colorless in fresh state
They are found in the blood stream, CT are pleomorphic
Total leucocytes count is 4000 to 11000 per cubic
millimeter
They are either Granulocytes or A GRANULOCYTES

14. Granulocytes
Neutrophils
Basophils
Eosinophils
A granulocytes
 Lymphocytes
Monocytes