Combining and improving phenotypic traits through the generation of synthetic two- and six-species yeast hybrids
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Generation of two- and six-species hybrids to generate genomic and phenotypic diversity useful for industry applications.
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Combining and improving phenotypic traits through the generation of synthetic two- and six-species yeast hybrids
- 1. Mechanisms of speciation and adaptation in Trichaptum abietinum David Peris Navarro Postdoctoral Researcher Department of Biosciences, UiO 20th February 2020 @DPerisN
- 2. David Peris, Postdoctoral Marie Curie Fellow Biotechnology Department, SBYBI Group 20th February 2020 @DPerisN Combining and improving phenotypic traits through the generation of synthetic two- and six-species yeast hybrids
- 3. Saccharomyces cerevisiae yeast model Image provided by: http://faculty.ccbcmd.edu/courses/bio141/lecguide/unit4/fungi/u1fig35.html Well characterize eukaryotic cell
- 4. Saccharomyces cerevisiae yeast model Image provided by: http://faculty.ccbcmd.edu/courses/bio141/lecguide/unit4/fungi/u1fig35.html Small genome Well characterize eukaryotic cell
- 5. Saccharomyces cerevisiae yeast model Image provided by: http://faculty.ccbcmd.edu/courses/bio141/lecguide/unit4/fungi/u1fig35.html Easy to manipulate Small genome Well characterize eukaryotic cell
- 6. Saccharomyces cerevisiae yeast model Image provided by: http://faculty.ccbcmd.edu/courses/bio141/lecguide/unit4/fungi/u1fig35.html Easy to manipulate Small genome Similarities to other eukaryotes Well characterize eukaryotic cell
- 7. Ability of S. cerevisiae strains to consume different carbon sources Carbon sources Glucose Fructose Glu Glu Maltose Glu Glu Glu Maltotriose
- 8. Domesticated S. cerevisiae strains Carbon sources Glucose Fructose Glu Glu Maltose Glu Glu Glu Maltotriose Ales Traditional beverages Wine Cider Sake Bakery
- 9. New industrial processes: biofuels Proteins, Oils, Ash (0-2%) Hemicellulose (19-34%) Lignin (21-32%) Cellulose (33-51%)
- 10. Wild S. cerevisiae can not metabolize xylose Proteins, Oils, Ash (0-2%) Hemicellulose (19-34%) Lignin (21-32%) Cellulose (33-51%) Glucose Xylose Sugars (C6/C5)
- 11. Hydrolysate toxins inhibit fermentation Proteins, Oils, Ash (0-2%) Hemicellulose (19-34%) Lignin (21-32%) Cellulose (33-51%) Glucose Xylose HMF Ferulic acid p-coumaric acid Feruloyl amide Sodium acetate Acetamide Sugars (C6/C5) Hydrolysate toxins
- 12. Re-domestication of S. cerevisiae The most tolerant of wild S. cerevisiae Engineered with xylose utilization genes Aaerobically evolved Y732n CHASSIS Xylose Hydrolysate toxins Wohlbach et al. 2009 PNAS Sato et al. 2013 AEM McIlwain, Peris, et al. 2016 G3
- 13. Genomic diversity across the genus Saccharomyces Peris et al in preparation Libkind, Peris et al 2020 FEMSYR S. cerevisiae S. paradoxus S. mikatae S. jurei S. kudriavzevii S. arboricola S. uvarum S. eubayanus Species
- 14. Phenotypic diversity across the genus Saccharomyces Wimalasena et al 2014 Microb Cell Factories Peris et al 2017 Biotech Biofuels S. cerevisiae S. paradoxus S. mikatae S. jurei S. kudriavzevii S. arboricola S. uvarum S. eubayanus Species AFEX Corn Stover Hydrolysate
- 15. Hybridization as a mechanism to improve chassis strains S. cerevisiae S. paradoxus S. mikatae S. jurei S. kudriavzevii S. arboricola S. uvarum S. eubayanus Species Langdon, Peris et al 2019 Nat Ecol & Evol Pontes et al 2019 Front Gen Peris et al 2018 Yeast Almeida et al 2014 Erny et al 2012 AEM Peris et al 2012 Yeast Peris et al 2012 BMC Genomics Peris et al 2012 PloS One Saaz/Group I Frohberg/Group II Olive Alpechin Champagne Cider Wine Belgium beers Some ales Txakoli Tokaj Amarone
- 16. Aims Generate higher order species hybrids
- 17. Diploids can not mate MAT/MATa MAT/MATa
- 18. iHyPr promotes homozigosity in the MAT locus MAT/MATa MAT/MATa MAT/MAT MATa/MATa
- 19. iHyPr for generation of allotetraploids MAT/MATa MAT/MATa MAT/MAT MATa/MATa X Alexander, Peris et al 2014 Fung Gen and Biol
- 20. Peris et al 2019 BioRxiv Langdon, Peris et al 2019 MBE iHyPr to generate six species hybrids
- 21. Aims Generate higher order species hybrids Select hybrids with different combinations of mitochondrial genomes
- 22. iHyPr to cross rho+ and rho0 strains MAT/MAT MATa/MATa X
- 23. iHyPr to select the mitochondrial inheritance X MAT/MAT MATa/MATa Baker, Peris et al 2019 Sci Advances Li, Peris et al 2019 Sci Advances
- 24. Aims Generate higher order species hybrids Select hybrids with different combinations of mitochondrial genomes Improve and retain parent phenotypic traits of interest through adaptive laboratory evolution
- 25. Please, come to visit my poster C3-05 How iHyPr works How genome size/ploidy correlates with fitness and cell volume The genome characterization of six-species hybrids The impact of mitochondrial inheritance in the genome and phenotypes The genome reduction and phenotypes improvement The retention of industrial interesting traits Future applications of iHyPr
- 26. Lainy Ramírez Aroca Amparo Querol David Lázaro Laura Pérez-Través Eladio Barrio José Guillamón Sergi Puig William G Alexander Mira G Basuino Emily J Ubbelohde Diego Libkind Jose Paulo Sampaio Paula Gonçalves Christian Landry Jean-Baptiste Leducq Guillaume Charron Justin Fay Katie Hyma Li Xueying Fengyan Bai Qi Ming Wang Chris T. Hittinger’s lab Quinn Langdon EmilyClaire Baker Rusell Wrobel Ryan Moriarty Kaitlin Fisher UW & GLBRC CollaborationSBYBI@IATA-CSIC Thank you