Attuluri Vamsi Kumar, Assistant Professor, Division: MTL, SMAS, Galgotias University, UP.

1. Experiment No - 7
Demonstration of virus cultures by embryonated egg
techniques
Sub code: BMLT5051
Sub Name: Virology and mycology Practical
Department: Department of MLT, SMAS
Faculty: A. Vamsi Kumar
Designation : Assistant professor
Sem- V

2. SYLLABUS OVERVIEW

3. Experiment No – 7
Demonstration of virus cultures by embryonated
egg techniques

4. Aim
To demonstrate embryonated egg for viral cultivation.

5. Materials required
1. wild chicken
eggs
2. Incubator
3. Disinfectants
4. Candle light
5. Egg holders
6. Cotton & Sprit
7. Egg Driller
7. Syringe
8. Discard tray
9. Wax seal
10. Discard tray
11. Markers
12. PPEs

7. Procedure cont..
Pocks in EE

8. Inoculation sites for various viruses

66. 1. https://www.barnsleyhospital.nhs.uk/pathology/microbiology/specimen-
collection/skin-scrapes-hair-and-nail-samples-for-mycology/
2. https://dermnetnz.org/topics/laboratory-tests-for-fungal-infection/
3. https://bpac.org.nz/BT/2011/March/fungal-infections.aspx
References