The document discusses David Peris's experiences as a Marie Curie Fellow in the biotechnology department, focusing on research involving mitochondrial genomes and hybrid strains. It outlines training, career development, and public engagement initiatives as part of the MSCA working packages while highlighting the challenges and achievements in his research. Key topics include mitochondrial inheritance, validation of hybrids, and insights gained from the process including pros and cons of the Marie Curie funding experience.

1. Developing your career with a MSCA IF - RI
2 years experience
David Peris, Postdoctoral Marie Curie Fellow
Biotechnology Department, SBYBI Group
26th June 2019
@djperis

2. MITOGRESSION origins
94% of hybrids with a non-S. cerevisiae mitochondrial genome
Peris et al 2016 Mol Phylo Evol

3. MITOGRESSION origins
94% of hybrids with a non-S. cerevisiae mitochondrial genome
Mitochondrial introgressions specific of Saccharomyces populations
Peris et al 2016 Mol Phylo Evol

4. MITOGRESSION origins
94% of hybrids with a non-S. cerevisiae mitochondrial genome
Mitochondrial introgressions specific of Saccharomyces populations
Phenotypic effects of mitochondrial inheritance
Spirek et al 2015 Frontiers Gen
Picazo et al 2014 Let App Microbiol
Paliwal et al 2014 Genetics

5. MITOGRESSION origins
94% of hybrids with a non-S. cerevisiae mitochondrial genome
Mitochondrial introgressions specific of Saccharomyces populations
Phenotypic effects of mitochondrial inheritance
Solve industrial challenges

6. Marie Curie proposal preparation
Late 2015

7. Marie Curie proposal preparation
Late 2015
Early 2016

8. Marie Curie proposal preparation
Late 2015
Early 2016
Mid 2016

9. Marie Curie proposal preparation
Late 2015
Early 2016
Mid 2016
September 2016
50%
30%
20%

10. MSCA working packages
Project Management
Training and Career Development
Dissemination and Public Engagement

11. WP1: Project management
Use the portal: continue reporting
Manage >600 strains
MTAs
Nagoya protocol
Access & Benefit Sharing (ABS) regulation

12. WP2: Training & career development
Personal Career Development (PCD) plan

13. WP2: Training & career development
Personal Career Development (PCD) plan
Courses
Systems Biology Phylogenomics
Metagenomics

14. WP2: Training & career development
Personal Career Development (PCD) plan
Courses (1)
Systems Biology Phylogenomics
Metagenomics

15. WP2: Training & career development
Personal Career Development (PCD) plan
Courses (1)
Systems Biology Phylogenomics
Metagenomics
Mentoring (7)
Undergrads Master

16. WP2: Training & career development
Personal Career Development (PCD) plan
Courses (1)
Systems Biology Phylogenomics
Metagenomics
Mentoring (7)
Undergrads Master

17. WP2: Training & career development
Personal Career Development (PCD) plan
Courses (1)
Systems Biology Phylogenomics
Metagenomics
Mentoring (7)
Maturity
Publications (14)
(7<1> + 3 + 4<1>)
Undergrads Master

18. WP2: Training & career development
Personal Career Development (PCD) plan
Courses (1)
Systems Biology Phylogenomics
Metagenomics
Mentoring (7)
Maturity
Grants (7)
2019 Retos
2017 GVA
2019 IVAFEPublications (14)
(7<1> + 3 + 4<1>)
Undergrads Master

19. WP2: Training & career development
Personal Career Development (PCD) plan
Courses (1)
Systems Biology Phylogenomics
Metagenomics
Mentoring (7)
Maturity
Grants (7)
2019 Retos
2017 GVA
2019 IVAFEPublications (14)
(7<1> + 3 + 4<1>)
Undergrads Master

20. WP2: Training & career development
Personal Career Development (PCD) plan
Courses (1)
Systems Biology Phylogenomics
Metagenomics
Mentoring (7)
Maturity
Grants (7)
2019 Retos
2017 GVA
Certification (2)
Associate
Lecturer
Associate
Professor
2019 IVAFEPublications (14)
(7<1> + 3 + 4<1>)
Undergrads Master

21. WP2: Training & career development
Personal Career Development (PCD) plan
Courses (1)
Systems Biology Phylogenomics
Metagenomics
Mentoring (7)
Undergrads Master
Maturity
Grants (7)
2019 Retos
2017 GVA
Certification (2)
Associate
Lecturer
Associate
Professor
2019 IVAFEPublications (14)
(7<1> + 3 + 4<1>)

22. WP2: Training & career development
Personal Career Development (PCD) plan
Courses (1)
Systems Biology Phylogenomics
Metagenomics
Mentoring (7)
Maturity
Grants (7)
2019 Retos
2017 GVA
Certification (2)
Associate
Lecturer
Associate
Professor
Leading (1)
Bioinformatic
Support
2019 IVAFEPublications (14)
(7<1> + 3 + 4<1>)
Undergrads Master

23. WP3: Dissemination and public engagement
Public Outreach
ExpociènciaFungus Olympics
ApuntDirecte News
Students
Forum Cinema
Climatic Change & EU Actions

24. WP3: Dissemination and public engagement
Seminar/Conferences
2017, 2019
2018
2018

25. WP3: Dissemination and public engagement
Press Notes

26. MSCA working packages
Project Management
Training and Career Development
Dissemination and Public Engagement
Industrial stress and Must fermentation
Experimental evolution
pre-zygotic and post-zygotic barriers
Mitochondrialinfluence

27. Research packages: challenges
600 new strains
3 co-author papers
1 First author paper
Securing independence

28. Research packages: challenges
Undergrads
PhDs
Postdocs -> Technician
rho0 strains
Hybrid construction: 14/16
Confirmation of hybrids: RFLP & mtDNA profile
Confirmation of ploidy: flow cytometry
Mitochondrial transfer to the shuttle strain: 1/3

29. Research packages: solutions
Master
Completed the set of hybrids: 2/16
Industrial stress and Must fermentation
Experimental evolution
Industrial stress and Phenotyping hybrids:
64 drop tests (32 x 2)
microtiter growth curves (11 x 3)
fermentations (16 + 3) x 2 x 3
GC, HPLC & Kinetics
Automatizing the quantification of drop test results
Adaptive Laboratory Evolution (target 200 generations)

30. Research packages: solutions
Mitochondrial affects thermotolerance
Lager hybrids inherited the low temperature tolerance from S. eubayanus
sppIDer: genomic characterization tool
Mitochondrial inheritance influences genomic output in interspecies hybrids
Baker, Peris et al 2019 Sci Adv
Li, Peris et al 2019 Sci Adv
Langdon, Peris et al MBE 2019
Peris et al 2019 BioRxiv

31. What did I learn about the MSCA?
Pros Cons
First opportunity for grant writing
Good salary
Best option to move
Great opportunity for training
Push to explore for solutions
Reputation
Low control in hiring people
Low budget for science
Short period of time for making
something productive
Non-stable position

32. Lainy Ramírez
Amparo Querol
Laura Pérez Través
Sebastián Tapia
Carla Perpiñá
David Lázaro
Mario Gambau
Dolores Pérez
Querol Lab Members
Eladio Barrio
Alejandro Aguilar
Maite Martínez
Sergi Puig
Raquel Sorribes
José Guillamón
Ricardo Bisquert
Chris T. Hittinger’s lab
EmilyClare Baker
Quinn Langdon
Russell Wrobel
Ryan Moriarty
Justin Fay
Li Xueying
Francisco Cubillos
CollaborationSBYBI