Collagen is the most abundant protein in the human body and provides structure and strength. It has a unique triple helix structure that gives tissues their tensile strength. There are over 20 types of collagen that are classified based on structure and function. Collagen synthesis involves transcription of genes, modification and assembly of procollagen monomers in the ER and Golgi, and extracellular processing and crosslinking to form mature collagen fibrils. Mutations in collagen genes or defects in processing can lead to heritable disorders like osteogenesis imperfecta or Ehlers-Danlos syndrome. Collagen supplements are used cosmetically to improve skin properties.
Extra cellular matrix is recently being explored in connection with cancer , metastases and autoimmune disorders. It is prepared for the benefit of both UG and PG medical and dental students.
What is Glycoprotein ?:
Glycoproteins are proteins that contain oligosaccharide chains (glycans) covalently attached to polypeptide side-chains.
This process is known as glycosylation.
The carbohydrate is attached to the protein during the following modifications: Co-translational modification & Post-translational modification.
In proteins that have segments extending extracellularly, the extracellular segments are often glycosylated.
Biochemistry of musculoskeletal system. biochemistry of MSS prepared by Fikad...fikaduseyoum1
biochemistry of MSS prepared by Fikadu Seyoum Tola. This ppt essentially discuss about collegen biosnthesis, defect and muscle energy metabolism with its regulations.
Extra cellular matrix is recently being explored in connection with cancer , metastases and autoimmune disorders. It is prepared for the benefit of both UG and PG medical and dental students.
What is Glycoprotein ?:
Glycoproteins are proteins that contain oligosaccharide chains (glycans) covalently attached to polypeptide side-chains.
This process is known as glycosylation.
The carbohydrate is attached to the protein during the following modifications: Co-translational modification & Post-translational modification.
In proteins that have segments extending extracellularly, the extracellular segments are often glycosylated.
Biochemistry of musculoskeletal system. biochemistry of MSS prepared by Fikad...fikaduseyoum1
biochemistry of MSS prepared by Fikadu Seyoum Tola. This ppt essentially discuss about collegen biosnthesis, defect and muscle energy metabolism with its regulations.
Collagen is most abundant protein in mammals, the main fibrous component of skin, bone, tendon and cartilage.
Collagen comprises one- third of the total protein, accounts for three-quarters of the dry weight of skin, and is the most prevalent component of the extracellular matrix.
The collagen family consists of 28 members and these are classified by Roman numbers on the basis of their chronology of discovery.
Collagen /certified fixed orthodontic courses by Indian dental academy Indian dental academy
Welcome to Indian Dental Academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and offering a wide range of dental certified courses in different formats.
Indian dental academy has a unique training program & curriculum that provides students with exceptional clinical skills and enabling them to return to their office with high level confidence and start treating patients
State of the art comprehensive training-Faculty of world wide repute &Very affordable.
Collagen / /certified fixed orthodontic courses by Indian dental academy Indian dental academy
Welcome to Indian Dental Academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and offering a wide range of dental certified courses in different formats.
Indian dental academy has a unique training program & curriculum that provides students with exceptional clinical skills and enabling them to return to their office with high level confidence and start treating patients
State of the art comprehensive training-Faculty of world wide repute &Very affordable.
Biochemical and clinical aspects of collagenrohini sane
A comprehensive presentation on Biochemical and clinical aspects of Collagen for MBBS, BDS, B Pharm & Biotechnology students to facilitate self- study.
Join live classes, download study aids, sell your documents, join or host your own classes online, get tutoring, tutor students, take practices tests and more at Examville.com
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
hematic appreciation test is a psychological assessment tool used to measure an individual's appreciation and understanding of specific themes or topics. This test helps to evaluate an individual's ability to connect different ideas and concepts within a given theme, as well as their overall comprehension and interpretation skills. The results of the test can provide valuable insights into an individual's cognitive abilities, creativity, and critical thinking skills
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
BREEDING METHODS FOR DISEASE RESISTANCE.pptxRASHMI M G
Plant breeding for disease resistance is a strategy to reduce crop losses caused by disease. Plants have an innate immune system that allows them to recognize pathogens and provide resistance. However, breeding for long-lasting resistance often involves combining multiple resistance genes
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Phenomics assisted breeding in crop improvementIshaGoswami9
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...Travis Hills MN
Travis Hills of Minnesota developed a method to convert waste into high-value dry fertilizer, significantly enriching soil quality. By providing farmers with a valuable resource derived from waste, Travis Hills helps enhance farm profitability while promoting environmental stewardship. Travis Hills' sustainable practices lead to cost savings and increased revenue for farmers by improving resource efficiency and reducing waste.
ESR spectroscopy in liquid food and beverages.pptxPRIYANKA PATEL
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
3. Evidence of regular structure was presented in 1930’s.
Ramachandrn, concentrated on the conformation of the
collagen monomer .
Dealing with the confirmation of each polypeptide chain gave the
triple helix “Madras” model.
Madras model is the most widely accepted model which gave the
quaternary structure of collagen
History:
4. INTRODUCTION
Collagen is a type of fibrous protein
Shares the common features of fibrous proteins like :
- Polypeptide chains organized approximately parallel along a
single axis
- Insoluble in water and dilute salt solution.
- Gives strength and flexibility to the structure.
Other examples
Elastin
Keratin.
5. Most abundant protein in human body
Makes 25% to 35% of whole body protein content.
Comes from Greek word “kolla” meaning “Glue producer”
Long rigid structure with three polypeptide chains
Forms rope like triple structure.
Present in tendons, ligaments, skin, cornea, cartilage, bone,
blood vessels, gut, intervertebral disc.
Variation in amino acid sequence defines properties
6. STRUCTURE
Tropocollagen is a basic structural unit
Consist of 3 polypeptide chains (α- chains) having about 1000
residues in each chain.
Each α- chain is left handed
Approximately 3 amino acid residue per turn.
300 nm long; 1.5 nm diameter.
Each of the 3 polypeptide chains are held in a helical
conformation by winding around each other.
7. 3 left-handed chains are super twisted about each other to
form a right-handed triple helix.
Opposite direction of coiling resists unwinding.
Super coils are stabilized by H- bonding between
individual polypeptide chain.
9. Amino acid sequence:
Amino acid sequence in collagen is unique.
Glycine occupies every third position.
Repetitive amino acid sequence may be represented
as Gly–X–Y, where X is frequently Proline and Y is
hydroxyproline or hydroxylysine.
10. • Proline facilitates the formation of the helical
conformation of each α-chain because its ring structure
causes "kinks“ in the peptide chain .
• Glysine fits into the restricted spaces where the three
chains of the helix come together.• Hydroxyproline occurs once in
about 10 amino acid residue.
• Hydroxylysine occurs once in
200 amino acid residue.
11. Hydoxyproline and
Hydroxylysine:
Residues result from hydroxylation of some of proline and
lysine residues.
Hydroxylation of proline gives hydroxyproline
Hydroxylation of lysine gives hydroxylysine.
These residues are rarely present in other proteins.
These are Post -translational modification
Hydroxyproline maximize H bond formation so increase
stabilization of triple helix.
12. Triple helix structure:
Tertiary structure of collagen.
Three helical polypeptide chains linked with each other by
H- bond to form triple helix.
Might be homotrimer or heterotrimer
Amino acid present on surface of the helical molecules
13.
14. Types of collagen:
More than 20 collagen types
Variations in the amino acid sequence of the α-
chains creates the differences
These α-chains are combined to form the various
types of collagen found in the tissues
For example:
The most common collagen , type I contains two
chains called alpha 1 and one chain called alpha 2
Whereas type II collagen contains three alpha 1
chains
15. The collagens can be organized into two groups
1. Fibrilar:
Type I, II, III , V ,VI
Rope like structure
Banding pattern in EM
It reflects the regular staggered packing of the
individual collagen molecules.
Type I: Tendon, Cornea
Type II: Cartilage
Type III: Blood vessels
19. Biosynthesis:
Polypeptide precursors of the collagen molecule are
formed in fibroblasts, osteoblasts, chondroblast
Secreted into the extracellular matrix
Enzymatic modification takes place forming the
mature collagen monomers
Aggregate and cross-linked to form collagen fibrils
20. Steps in biosynthesis:
Transcription of mRNA
Pre- Pro peptide Formation
Pre –Pro peptide to procollagen
Golgi Apparatus modification
Formation of tropocollagen
Formation of collagen fibril
21. Pre–Pro Peptide Formation
• About 34 genes are associated for coding specific mRNA
sequence.
• mRNA then exits from nucleus, enters cytoplasm.
• Then it links with ribosomal subunit for translation.
• Translation produce Pre pro peptide or pre pro alpha chain.
• N terminal of this peptide has special amino acid sequence
called signal sequence
• This is recognized by the a signal recognition particle in
endoplasmic reticulum.
• Directs the peptide to ER.
22. Formation Of Procollagen
Three modifications of the pre-pro-peptide occur leading to the
formation of the alpha peptide:
The signal peptide on the N-terminal is dissolved, and the molecule
is now known as propeptide (not procollagen).
Hydroxylation of lysines and by the enzymes 'prolyl hydroxylase'
and 'lysyl hydroxylase'
Glycosylation occurs by adding either glucose or galactose
monomers onto the hydroxyl groups that were placed onto lysines,
but not on prolines.
Three of the hydroxylated and glycosylated propeptides twist into a
triple helix forming procollagen. Procollagen still has unwound
ends, which will be later trimmed.
23. Hydroxylation:
Proline and lysine residues found in the Y-position of the Gly-
X-Y- sequence can be hydroxylated to form hydroxyproline
and hydroxylysine residues.
Hydroxyproline stabilize the triple helix.
Hydroxylysine permits cross linking of helices to form fibres.
Hydroxylation is enzymatic reaction with ascorbic acid as co-
factor.
Ascorbate or ascorbic acid increase synthesis of collagen by
8 folds. (Murad et.al).
24. Glycosylation:
The hydroxyl group of the hydroxylysine residues of
collagen may be enzymatically glycosylated.
Glucose and Galactose are attached to polypeptide
chains
This addition results in twisting of the chain.
25. Golgi Apparatus Modification :
Three helical pro-α-chains assemble.
Forms Pro–collagen with twisted portion at center and loose
ends on either sides.
The Pro-collagen molecules are then translocated to the Golgi
apparatus, where they are attached to oligosaccharides and
packaged in secretory vesicles.
The vesicles fuse with the cell membrane, causing the release
of Pro-collagen molecules into the extracellular space.
26. Formation of Tropocollagen
• The procollagen molecules then are cleaved by N- and C-
pro-collagen peptidases, which remove the terminal
propeptides, releasing triple-helical molecules.
• This molecule is termed as tropocollagen.
• Absent when synthesizing the type III (Fibrillar collagen )
27.
28. Formation of collagen fibrils:
Individual collagen molecules spontaneously associate to form
fibrils.
They form an ordered, overlapping, parallel array, with
adjacent collagen molecules arranged in a staggered pattern,
each overlapping its neighbour by a length approximately
three-quarters of a molecule.
Called “ Quarter staggered arrangement’’
29. Cross-link formation:
• The fibrillar array of collagen molecules serves as a substrate
for lysyl oxidase.
• This extracellular enzyme oxidatively deaminates some of the
lysyl and hydroxylysyl residues in collagen.
• The reactive aldehydes that result (allysine and
hydroxyallysine) can condense with lysyl or hydroxylysyl
residues in neighboring collagen molecules to form covalent
cross-links .
The cross-linking is essential for achieving the tensile strength
necessary for proper functioning of the connective tissue.
30.
31.
32. Degradation of collagen
Highly stable; t1/2 several months
Remodeled in response to growth or tissue injury
Breakdown depends on proteolytic action of collagenase
The organism Clostridium histolyticum conatins the
enzyme collagenase
Cleave collagen fibres into smaller fragments that can be
phagocytosed and further degraded by lysosomal
enzymes to their constituent amino acids.
34. Medical importance:
Cosmetic surgery.
Bone grafting
Construction of artificial skin substitutes
Hemostatic properties
35. Collagen Abnormalities:
Can be due to:
Defect in expression of collagen gene.
Molecular defect in processing/synthesis
Defect in structure of collagen
36. Scurvy
Resulting from Vitmin C deficiency
Presented as malaise , lethargy , spongy gums ,
bleeding from the mucous membranes
The reaction affected is the hydrxylation of proline
and lysine
InterchaingH bond formation impaired
Capillary fragility leads to sub cutaneous
extravasations of blood
37. Osteogenesis imperfecta:
• Also known as brittle bone syndrome
• Heterogenous group of inherited disorders.
• Mutation in COL1A1 and COL1A2
• Replacement of single Glycine residue by
bulkier amino acid in Type I collagen.
• Symptoms depends on types
• Blue sclera, loss of hearing, retarded wound
healing and a rotated and twisted spine leading
to "humped-back" appearance are common
features
38. Pathophysiology based on the steric hindrance created
by the bulge produced due to bulkier amino acids
There are around 8 types of OI but type 1 is more
common
Type I- Present in early infancy with features
secondary to trauma
Type II-More severe , pulmonary hypoplasia in utero
or during the neonate
39. Ehlers-Danlos Syndrome (EDS):
Inherited connective tissue disorder.
Result from a deficiency of collagen-processing enzymes
(e.g, lysyl oxidase or pro-collagen peptidase), or from
mutations in the amino acid sequences of collagen.
Mutation in genes COL1A1, COL1A2, COL3A1,
COL5A1, COL5A2
Alters the structure, production or processing of
collagen.
40. Signs and symptoms:
Hyper flexibility of joints
Unstable joints
Osteopenia
Stretching and fragile skin
Easy bruising and poor wound healing
Cardiovascular disorders
Valvular disease
Aneurysm
41. Some of the lysyl side chains of the tropoelastin
polypeptides are oxidatively deaminated by lysyl
oxidase, forming allysine residues.
Three of the allysyl side chains plus one unaltered lysyl
side chain from the same or neighboring polypeptides
form a desmosine cross-link .
This produces elastin- an extensively interconnected,
rubbery network that can stretch and bend in any direction
when stressed, giving connective tissue elasticity.
42. Nicolos Paganini Italian violinist had EDS
This rare syndrome helped him play the violin
extraordinarily
The India rubber man , elastic lady are
other personalities described in history having
EDS
43. Alport Syndrome
Is the designation applied to a number of genetic
disorders (both X-linked and autosomal) affecting the
structure of type IV collagen fibers
The presenting sign is hematuria, and patients may
eventually develop end-stage renal disease
Characteristic abnormalities of the structure of the
basement membrane and lamina
44. Epidermolysis Bullosa
Mutations in COL7A1
Presented as skin breaks and blisters as a result
of minor trauma
VII collagen is responsible for anchoring the basal
lamina to collagen fibril in dermis
Anchoring fibrils are reduced
48. Cosmetics and Collagen
Fish collagen peptides are used extensively
The products claims to increase in the total collagen in
body
To improve skin elasticity , smoothness and moisture
Some articles have proved to reduce the wrinkles by
wrinkle replica analysis ( Bourmand et al )
49. References:
Lehninger-Principles of Biochemistry 5th edition.
Lippincott’s Biochemistry 4th edition.
Harper’s Illustrated Biochemistry 26th edition.
Harrison’s Principle of Internal Medicine 17th edition.
www.authorstream.com
Balasubramanian, D . (October 2001). "GNR — A Tribute". Resonance.
Indian Academy of Sciences. 6 (10). Archived from the original on 2014-
01-10.
Beighton, P. (1970) The Ehlers-Danlos Syndrome. William Heinemann
Medical Books Ltd, pp. 1–194. Beighton, P. (1993) The Ehlers-Danlos
syndrome. In: Mckusi
Borumand M, Sibilla S. Effects of a nutritional
supplement containing collagen peptides on skin
elasticity, hydration and wrinkles. J Med Nutr Nutraceut
2015;4:47-53