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IN VITRO EVALUATION  THE CITREX® MOLECULE  INHIBITORY/CIDAL ACTIVITY  AGAINST DIFFERENT ORGANISMS HAROLD EDUARDO DURANGO G. Medical Mycology Bacteriologist Professor of Microbiology and Parasitology. Medicine Faculty, U. of A. JOSE IGNACIO BARGUIL P. Food Microbiology Bacteriologist Food protection technical consultant, Consultant for Banco Interamericano de Desarrollo, BID. Technical Director, Continua Consultores. Pediatric Infectology Research Laboratory. H.U.S.V.P
Microbiological assays using CITREX ®  liquid ,[object Object]
Organisms challenged ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
CITREX ®  concentrations tested Tube number Citrex® Concentration (ppm) Tube 1 1000 Tube 2 800 Tube 3 400 Tube 4 200 Tube 5 100 Tube 6 50 Tube 7 25 Tube 8 12.5 Tube 9 6.25
Process: ,[object Object],[object Object],[object Object]
Process: ,[object Object]
Controls for test validation ,[object Object],[object Object],[object Object]
CITREX ® Minimum Inhibitory Concentrations Test 1: Microorganism CITREX 1000 ppm CITREX 800 ppm CITREX 400 ppm CITREX 200 ppm CITREX 100 ppm CITREX 50 ppm CITREX 25 ppm CITREX 12.5 ppm CITREX 6.25 ppm P. aeruginosa - - + + + + + + + E. coli - - - - + + + + + S. typhimurium - - - - + + + + + V. cholerae - - - - + + + + + S. aureus - - - - - - - - + E. faecalis - - - - - - - + + C. albicans - - - - - - - + + A. flavus - - - - - - + + +
CITREX ® Minimum Inhibitory Concentrations Test 2: + + + - - - - - - A. flavus + - - - - - - - - C. albicans + - - - - - - - - E. faecalis + - - - - - - - - S. aureus + + + + + - - - - V. cholerae + + + + + - - - - S. typhimurium + + + + + - - - - E. coli + + + + + + + - - P. aeruginosa CITREX 6.25 ppm CITREX 12.5 ppm CITREX 25 ppm CITREX 50 ppm CITREX 100 ppm CITREX 200 ppm CITREX 400 ppm CITREX 800 ppm CITREX 1000 ppm Microorganism
Test 3: CITREX ® Minimum Inhibitory Concentrations Microorganism CITREX 1000 ppm CITREX 800 ppm CITREX 400 ppm CITREX 200 ppm CITREX 100 ppm CITREX 50 ppm CITREX 25 ppm CITREX 12.5 ppm CITREX 6.25 ppm P. aeruginosa - - + + + + + + + E. coli - - - - + + + + + S. typhimurium - - - - + + + + + V. cholerae - - - - + + + + + S. aureus - - - - - - - - + E. faecalis - - - - - - - - + C. albicans - - - - - - - + + A. flavus - - - - - - + + +
Result summary from all 3 tests *  Corresponds to MIC in 2 of 3 tests Microorganism Minimum Inhibitory Concentration (ppm CITREX ®) P. aeruginosa 800 E. coli 200 S. typhimurium 200 V. cholerae 200 S. aureus 12.5 E. faecalis 12.5 – 25* C. albicans 12.5 – 25* A. flavus 50
MINIMUM BACTERICIDAL CONCENTRATION ,[object Object],[object Object]
CITREX  ® Liquid Minimal Bactericidal Concentration 200 A. flavus 50 C. albicans 50 E. faecalis 25 S. aureus 400 V. cholerae 400 S. typhimurium 800 E. coli 1000 P. aeruginosa Minimum Bactericidal Concentration (ppm) Microorganism
RESULT DISCUSSION  ,[object Object],[object Object]
RESULT DISCUSSION  ,[object Object],[object Object]

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Citrex610 i-en-revised spa 1 citrex mic - mbc

  • 1. IN VITRO EVALUATION THE CITREX® MOLECULE INHIBITORY/CIDAL ACTIVITY AGAINST DIFFERENT ORGANISMS HAROLD EDUARDO DURANGO G. Medical Mycology Bacteriologist Professor of Microbiology and Parasitology. Medicine Faculty, U. of A. JOSE IGNACIO BARGUIL P. Food Microbiology Bacteriologist Food protection technical consultant, Consultant for Banco Interamericano de Desarrollo, BID. Technical Director, Continua Consultores. Pediatric Infectology Research Laboratory. H.U.S.V.P
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  • 4. CITREX ® concentrations tested Tube number Citrex® Concentration (ppm) Tube 1 1000 Tube 2 800 Tube 3 400 Tube 4 200 Tube 5 100 Tube 6 50 Tube 7 25 Tube 8 12.5 Tube 9 6.25
  • 5.
  • 6.
  • 7.
  • 8. CITREX ® Minimum Inhibitory Concentrations Test 1: Microorganism CITREX 1000 ppm CITREX 800 ppm CITREX 400 ppm CITREX 200 ppm CITREX 100 ppm CITREX 50 ppm CITREX 25 ppm CITREX 12.5 ppm CITREX 6.25 ppm P. aeruginosa - - + + + + + + + E. coli - - - - + + + + + S. typhimurium - - - - + + + + + V. cholerae - - - - + + + + + S. aureus - - - - - - - - + E. faecalis - - - - - - - + + C. albicans - - - - - - - + + A. flavus - - - - - - + + +
  • 9. CITREX ® Minimum Inhibitory Concentrations Test 2: + + + - - - - - - A. flavus + - - - - - - - - C. albicans + - - - - - - - - E. faecalis + - - - - - - - - S. aureus + + + + + - - - - V. cholerae + + + + + - - - - S. typhimurium + + + + + - - - - E. coli + + + + + + + - - P. aeruginosa CITREX 6.25 ppm CITREX 12.5 ppm CITREX 25 ppm CITREX 50 ppm CITREX 100 ppm CITREX 200 ppm CITREX 400 ppm CITREX 800 ppm CITREX 1000 ppm Microorganism
  • 10. Test 3: CITREX ® Minimum Inhibitory Concentrations Microorganism CITREX 1000 ppm CITREX 800 ppm CITREX 400 ppm CITREX 200 ppm CITREX 100 ppm CITREX 50 ppm CITREX 25 ppm CITREX 12.5 ppm CITREX 6.25 ppm P. aeruginosa - - + + + + + + + E. coli - - - - + + + + + S. typhimurium - - - - + + + + + V. cholerae - - - - + + + + + S. aureus - - - - - - - - + E. faecalis - - - - - - - - + C. albicans - - - - - - - + + A. flavus - - - - - - + + +
  • 11. Result summary from all 3 tests * Corresponds to MIC in 2 of 3 tests Microorganism Minimum Inhibitory Concentration (ppm CITREX ®) P. aeruginosa 800 E. coli 200 S. typhimurium 200 V. cholerae 200 S. aureus 12.5 E. faecalis 12.5 – 25* C. albicans 12.5 – 25* A. flavus 50
  • 12.
  • 13. CITREX ® Liquid Minimal Bactericidal Concentration 200 A. flavus 50 C. albicans 50 E. faecalis 25 S. aureus 400 V. cholerae 400 S. typhimurium 800 E. coli 1000 P. aeruginosa Minimum Bactericidal Concentration (ppm) Microorganism
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  • 15.