chemotaxis in bacteria

1. By: Krishna Kant Yadav Chemotaxis

2. CheW CheY CheY CheY CheY CheY CheY MCP-Methyl accepting chemotaxis protein CheA repellent

3. CheW CheY CheY CheY CheY CheY CheY CheA Che A get phosphorylated

4. CheW CheY CheY CheY CheY CheY CheY CheA

5. CheW CheY CheY CheY CheY CheY CheY CheA

6. CheW CheY CheY CheY CheY CheY CheY CheA Che Y get phosphorylated as a result its CheY-P conacentration increases in the cell

7. CheW CheY CheY CheY CheY CheY CheY CheA Phosphorylated Che Y interact with the motor of flagella

8. CheW CheY CheY CheY CheY CheY CheY CheA Induces clockwise rotation Repellent increase CheY-P= tumbling motion Attractant lowers CheY-P = smooth motion

9. CheW CheY CheY CheY CheY CheY CheY CheA Che Z

10. Repellent Attractant Results in clockwise rotation (Tumbling) Binding to MCP Low level CheA-P&CheY-P Less stimulation of the pathway CheY-P interact with Flagellar motor Che W CheA-P CheA CheY CheY-P Binding to MCP Counterclockwise rotation (Smooth Run)

11. Adaptation

15. CheW CheY CheY CheY CheA Che Z CheA CheR Che B Che B Met Met Binding of attractant

16. CheW CheY CheY CheY CheA Che Z CheA CheR Che B Che B Met Met Change in conformation of MCP Less CheA-P Smooth motion Less CheB-P Methylation occurs

17. CheW CheY CheY CheY CheA Che Z CheA CheR Che B Che B Met Met Change in conformation of MCP Less CheA-P Smooth motion Less CheB-P Methylation occurs MCP returns to its original form CheA-P & Che Y-P starts increasing Tumbling occurs More CheB-P synthesised Demethylation occurs

18. Adaptation Binding of attractant or In absence of attractant Decreased activity of MCP Low Che Y-P smooth run High methylation of MCP by Che R MCP returns to original confirmation Tumblig occurs At the same time Che B—P level increases and it demethylates MCP Increase level of Che Y-P