Chapter 3 Bacterial Staining and Culture Media

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This presentation described the different kinds of staining procedure and culture media needed for certain type of microorganism

Reagent Function Result if Gram-positive Result if Gram-negative
Crystal volet Primary stain Purple or Blue Purple or Blue
Gram’s iodine Mordant Purple or Blue Purple or Blue
Acetone or 95% alcohol Decolorizer Purple or Blue Colorless
Safranin Counterstain or
secondary stain
Purple or Blue Red or Pink

Reagent Function Result
Ziehl-Neelsen Kinyoun Acid-fast Non-acid fast
Carbol fuchsin Carbol fuchsin Primary stain Red or Pink Red or Pink
Acid alcohol Acid alcohol Decolorizer Red Colorless
Methylene Blue Malachite Green Counterstain or
Secondary Stain
Ziehl-Neelsen:
red
organism/blue
background
Kinyoun: red
organism/green
background
Ziehl-Neelsen:
blue
organism/blue
background
Kinyoun: green
organism/green
background

Chapter 3 Bacterial Staining and Culture Media

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Chapter 3 Bacterial Staining and Culture Media

1. BACTERIAL STAINING AND CULTURE MEDIA

3.   

6.   1.     

9. Reagent Function Result if Gram-positive Result if Gram-negative Crystal volet Primary stain Purple or Blue Purple or Blue Gram’s iodine Mordant Purple or Blue Purple or Blue Acetone or 95% alcohol Decolorizer Purple or Blue Colorless Safranin Counterstain or secondary stain Purple or Blue Red or Pink

10.   a. b.

11.

12.

13. Reagent Function Result Ziehl-Neelsen Kinyoun Acid-fast Non-acid fast Carbol fuchsin Carbol fuchsin Primary stain Red or Pink Red or Pink Acid alcohol Acid alcohol Decolorizer Red Colorless Methylene Blue Malachite Green Counterstain or Secondary Stain Ziehl-Neelsen: red organism/blue background Kinyoun: red organism/green background Ziehl-Neelsen: blue organism/blue background Kinyoun: green organism/green background

14.

15.       

16.

17.

20.  

23.   

24. 1.  

25.   