Eukaryotic cells have internal membranes that compartmentalize their functions, unlike prokaryotic cells. Eukaryotic cells are characterized by having DNA contained within a nucleus, membrane-bound organelles, and a cytoplasm divided into the region inside and outside the nucleus. In contrast, prokaryotic cells lack a nucleus and organelles, and have their DNA located in the nucleoid region of the cytoplasm. A eukaryotic cell uses internal membranes to partition the cell into membrane-bound organelles that carry out specialized functions.