The document discusses the eukaryotic cell cycle, including key stages like mitosis and cytokinesis. It describes processes like centrosome duplication, microtubule movement, chromosome capture and separation, and spindle elongation. Regulatory mechanisms involving proteins like cyclins, CDKs, and ubiquitin ligases that control the cell cycle through phosphorylation, gene expression, and protein degradation are examined. Research on cell cycle regulation in organisms like yeast, sea urchins, Xenopus, and mammals is summarized.

1. The eukaryotic cell cycle

2. Mitosis and cytokinesis

3. Blue-DNA Green-microtubule

4. Fig. 19-36
Mitotic apparatus
of mammalian cells

5. Centrosome duplication and cell cycle

6. Microtubule motors and centrosome
movement at prophase

7. Fig. 19-43

8. Capture chromosomes
and stabilizing
chromosomes at the
equatorial plate in metaphase

9. Shortening at the (+) end
of kinetochore microtubules
moves chromosomes
poleward in anaphase A

10. Spindle elongation and movement of poles during anaphase B

11. Whether does the spindle or the asters
control location of the cleavage plane during cytokinesis?

12. Regulation of myosin during cytokinesis
MPF-mitosis promoting factor

13. Mitosis in a higher plant cell

14. Regulation of cell cycle
Protein factors
Cyclin
CDK-cyclin-dependent kinase
Cyclin complex
G1 cyclin-CDK complexes
S phase cyclin-CDK complex
Mitotic cyclin-CDK complex
Cell cycle inhibitors
Cell cycle promoting factor -
ubiquitin ligase lead to degradation
of cell cycle inhibitors
SCF and APC
Mechanisms
Phosphorylation and
dephosphorylation
Activation of gene
transcription and translation
Proteasome-mediated
degradation

15. Current model for
regulation of the
eukaryotic cell cycle
CDK-cyclin-dependent kinase
SCF-ubiquitin ligase
APC-anaphase promoting complex

16. A diffusible factor in arrested Xenopus eggs promotes meiotic maturation

17. Biochemical detection of cyclical synthesis and destruction of mitotic cyclin
in sea urchin embryos

18. Isolation of cell-division cycle (CDC) genes from a S. cerevisiae
genomic library by functional complementation of cdc mutants

19. Using S. pombe cdc mutants to study the cell cycle regulation

20. Regulation of the kinase
activity of S. pombe mitosis-
promoting factor by
phosphorylation and
dephosphorylation

25. Depolymerization of the nuclear lamina

26. The phosphorylation of human lamin a is required for lamin
depolymerization

27. Control of entry into anaphase by APC-regulated degradation of
the cohesin link between sister chromatides

28. The reassembly of nuclear
envelop during telophase

29. Mammalian cyclin-CDK complexes

30. Using bromodeoxyuridin (BrdU) to test the
function of cyclin D in mammalian cells

31. Checkpoint controls in cell cycle

32. Role of p53 in cell cycle check points