Centrifugation is used to separate materials of different densities using centrifugal force greater than gravity. It is commonly used in industrial processes like insulin production to separate liquid phases and solids. There are different types of centrifuges like basket, disc-stack, and tubular bowl centrifuges. Basket centrifuges are useful for separating mycelia while disc-stack centrifuges can separate cellular debris from viable cells. Centrifugation has many applications including dairy processing, oil and wastewater treatment, and blood separation into components. Key factors affecting centrifugation include feed rate, rotational speed, particle size and density difference between materials to be separated.
this presentation is all about how the parameters are that are controlled in a bioreactor. it is one of the important chapter in bio process engineering.
this presentation is all about how the parameters are that are controlled in a bioreactor. it is one of the important chapter in bio process engineering.
Steps involved in fermentation products producing a viable product output.various steps and process were explained in them. A semester syllabus of undergraduate microbiology student in his/her semester -5 in paper -6 . I think this might be helpful to you and have a good response after reading this .thank you.
The heart of the fermentation or bioprocess technology is the Fermentor or Bioreactor. A bioreactor is basically a device in which the organisms are cultivated to form the desired products. it is a containment system designed to give right environment for optimal growth and metabolic activity of the organism.
A fermentor usually refers to the containment system for the cultivation of prokaryotic cells, while a bioreactor grows the eukaryotic cells (mammalian, insect cells, etc).
Scale up means increasing the quantity or volume of cell culture. For animal cells, the scale up strategies are dependent upon cell types or i.e. whether the cells requires matrix for attachment and growth ( adherent cell culture) or grows freely in suspended form in aqueous media. The scaling up principle for adherent cells are just to increase surface area for attachment while for suspension culture is to increase culture volume. This presentation enlightens the reader about different methods of scaling up of cells culture. Readers are also provided with sample questions for better understanding
Batch and Continuous Sterilization of Media in Fermentation Industry Dr. Pavan Kundur
Continuous sterilization is the rapid transfer of heat to medium through steam condensate without the use of a heat exchanger. ... This is more efficient than batch sterilization because instead of expending energy to heat, hold, and cool the entire system, small portions of the inlet streams are heated at a time.
Steps involved in fermentation products producing a viable product output.various steps and process were explained in them. A semester syllabus of undergraduate microbiology student in his/her semester -5 in paper -6 . I think this might be helpful to you and have a good response after reading this .thank you.
The heart of the fermentation or bioprocess technology is the Fermentor or Bioreactor. A bioreactor is basically a device in which the organisms are cultivated to form the desired products. it is a containment system designed to give right environment for optimal growth and metabolic activity of the organism.
A fermentor usually refers to the containment system for the cultivation of prokaryotic cells, while a bioreactor grows the eukaryotic cells (mammalian, insect cells, etc).
Scale up means increasing the quantity or volume of cell culture. For animal cells, the scale up strategies are dependent upon cell types or i.e. whether the cells requires matrix for attachment and growth ( adherent cell culture) or grows freely in suspended form in aqueous media. The scaling up principle for adherent cells are just to increase surface area for attachment while for suspension culture is to increase culture volume. This presentation enlightens the reader about different methods of scaling up of cells culture. Readers are also provided with sample questions for better understanding
Batch and Continuous Sterilization of Media in Fermentation Industry Dr. Pavan Kundur
Continuous sterilization is the rapid transfer of heat to medium through steam condensate without the use of a heat exchanger. ... This is more efficient than batch sterilization because instead of expending energy to heat, hold, and cool the entire system, small portions of the inlet streams are heated at a time.
Dissolution is a process in which a solid substance solubilizes in a given solvent.
Method for dissolution are-
1. Beaker methods
2. Open flow through compartment system
3.Dialysis concept
Centrifugation principle and types by Dr. Anurag YadavDr Anurag Yadav
concept of cnetrifugation,
basic Principle
centrifugal force
types of centrifugation based on use and rotor type
application of the each type of centrifuge
Ultracentrifuge in detail
application in general
Objectives, principle & applications of CENTRIFUGATIONAkankshaPatel55
Centrifugation is another important technique for separating components in a mixture, and it differs from filtration in some key ways. Here's a breakdown:
Centrifugation:
Principle: Utilizes centrifugal force, generated by spinning a sample at high speeds, to separate components based on their size, shape, density, and viscosity of the surrounding medium.
Process: Samples are placed in tubes and spun within a centrifuge rotor. Denser components migrate outwards, forming a pellet at the bottom, while lighter components remain closer to the center as supernatant.
Applications: Widely used in biology, chemistry, medicine, biotechnology, and other fields for separating blood cells, organelles, proteins, DNA, and more.
Types: Various types exist, like fixed-angle, swinging-bucket, gradient centrifugation, each with specific functionalities and applications.
actors influencing the process:
Centrifuge speed: Higher speeds generate greater centrifugal force, enabling separation of smaller and less dense particles.
Time: Longer centrifugation times allow for more complete separation, but may not be necessary for all mixtures.
Temperature: Certain separations require specific temperature control to maintain sample integrity or optimize separation efficiency.
Sample properties: Size, shape, and density of the components in the mixture significantly impact their separation behavior.
Rotor type: Different rotors have specific capacities, speeds, and applications. Choosing the right rotor is crucial for optimal results.
It is a brief presentation made on Biomagnification at different tropic levels. Accumulation of toxic materials over time shows a drastic change and leads to severe consequences. Extinction of species is one of them.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Toxic effects of heavy metals : Lead and Arsenicsanjana502982
Heavy metals are naturally occuring metallic chemical elements that have relatively high density, and are toxic at even low concentrations. All toxic metals are termed as heavy metals irrespective of their atomic mass and density, eg. arsenic, lead, mercury, cadmium, thallium, chromium, etc.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Salas, V. (2024) "John of St. Thomas (Poinsot) on the Science of Sacred Theol...Studia Poinsotiana
I Introduction
II Subalternation and Theology
III Theology and Dogmatic Declarations
IV The Mixed Principles of Theology
V Virtual Revelation: The Unity of Theology
VI Theology as a Natural Science
VII Theology’s Certitude
VIII Conclusion
Notes
Bibliography
All the contents are fully attributable to the author, Doctor Victor Salas. Should you wish to get this text republished, get in touch with the author or the editorial committee of the Studia Poinsotiana. Insofar as possible, we will be happy to broker your contact.
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
3. The various stages of processing that occur after the
completion of the fermentation or bioconversion stage,
including separation, purification, and packaging of the
product.
4. Centrifugation is used to separate materials of different density when a force
greater than gravity is desired.
Centrifugation method is utilized to separate the cellular debris from the released
protein.
Example-a key role in many industrial processes, including the production of
insulin, is to separate liquid phases and solids from each other.
It depends on particles size, density difference between the cells and the broth and
broth viscosity.
It is based on the behavior of particles in an applied centrifugal field.
More dense components of the mixture move away from the axis of the centrifuge
while less dense components of the mixture move towards the axis
5. 5
Centrifugation is used to separate materials of different density when a
force greater than gravity is desired.
The particles will tend to sediment under the influence of gravity.
If the particles suspended in a liquid are so small or have a density so
close to that of the liquid, then the force of gravity fails to sediment the
particles into a separate layer.
So the basis of centrifugation techniques is to exert a larger force than
the gravitational force to enhance the effective sedimentation force for
the separating such particles from the liquid.
Particles which differ in density, shape or size can be separated since
they sediment at different rates in the centrifugal field, each particle
sedimenting at a rate which is proportional to the applied centrifugal
field.
6. The rate at which the sedimentation occurs in centrifugation is expressed in
terms of sedimentation coefficient and is given by the formula:
Where, V = sedimentation of the molecules
ω = Rotation of the rotor in radians/sec.
(angular velocity)
r = Distance in cm, from the centre of
the rotor
S = V/ω2r
7. Bowl Basket Centrifuge- Useful for separating mould mycelia or crystalline
compounds.
Perforated & Lined with a filter bag of nylon, cotton.
Normally operated at speed of up to 4000 rpm for feed rate 50-300 dm³ min¯¹.
Continuous feed is used & when the basket is filled with the filter cake it is
possible to wash the cake before removing it.
It may be considered to be a centrifugal filter and holding capacity is 50-300 dm³
Blinding with soft biological materials so that high centrifugal forces cannot be
used.
8. Disc-stack bowl centrifuge- This type is common in bioprocess. The developed forces is 5000-15000 G
with minimal density difference between solid and liquid is 0.01-0.03 kg/m3.
Large particles have higher settling velocities than small particles
Cellular debris ends up at the outer edge of the bowl
Soluble intracellular material passes through with the clarified liquid
The minimum particle diameter is 5 µm.
The discs split the stream into a large number of very thin layers thereby improving separation.
Solids flow downwards on bottom face of disc.
Liquid flows upwards on top face of disc.
The close packing of the discs assists rapid sedimentation and the solids then slides to the edge of the bowl.
Smaller in size compared with a bowl without discs for given throughput.
Requires in-situ steam sterilization and the discs arrangement makes this type of centrifuge laborious to
clean.
Feed rate 45-1800 dm³ min¯¹.
9. Tubular bowl centrifuge (Narrow tubular bowl centrifuge or ultracentrifuge, decanter
centrifuge)-Used for particle size ranging from 0.1-200 µm.
Simple and widely applied in food and pharmaceutical industry. Operates at 13000-16000 G, 105-
106 G for ultracentrifuge.
Feed containing solids, heavy liquid phases are introduced by nozzle and are kept separate in their
exit from the bowl by an adjustable ring.
High centrifugal force, good dewatering and ease of cleaning and plastic liners can be used in the
bowls to help improve batch cycle time.
It can be employed for Light phase/heavy phase liquid separation, Solid/light-liquid phase/heavy-
liquid phase separation.
Disadvantages re limited solids capacity, difficulties in the recovery of collected solids, gradual
loss in efficiency as the bowl fills, and foaming.
10. The clarification efficiency of centrifugation process is affected by
harvest parameters such as centrifuge feed rate, G-force, bowl
geometry, operating pressure, discharge frequency and ancillary
equipment used in transfer of cell culture fluid.
Peak cell density, total cell density and culture viability during the
culture process and harvest will also affect separation performance.
Particles of submicron size cannot be removed in the centrifuge,
thus increases the burden on subsequent depth filtration.
Disk stack continuous centrifuge removes cell debris from viable
cells and liquid phase but some cells gets disrupted during process
especially feedstock with low viability culture fluid
11. Optimization of centrifugation process can be done at lab scale and pilot to select
the feed rate and bowl rotational speed using the scaling factors of feed rate (Q) and
equivalent settling area
As lower the value of sigma factor the better will be the clarification and it should
be govern that Q/ better will be the separation.
Feed rate containing fragile cells, viable cells are to be separated at lower speed
with constant value of sigma factor
12. Centrifuges are basically employed for separation of whole large cells from heterogeneous
cell mixture.
Can be used to separate viable cells from cell debris using disc stack centrifuge.
clarification (removal of solid impurities from milk prior to pasteurization)
skimming (separation of cream from skim milk)
standardizing
whey separation (separation of whey cream (fat) from whey)
bactofuge treatment (separation of bacteria from milk)
quark separation (separation of quarg curd from whey)
butter oil purification (separation of serum phase from anhydrous milk fat).
Disc-stack centrifuges used by some companies in the oil sands industry to separate small
amounts of water and solids from bitumen.
Large industrial centrifuges are commonly used in water and wastewater treatment to
dry sludges. The resulting dry product is often termed cake, and the water leaving a centrifuge
after most of the solids have been removed is called centrate.
Large industrial centrifuges are also used in the oil industry to remove solids from the drilling
fluid.
Centrifuge are also used in whole blood separation into different components.
14. Tube
◦ High centrifugal force
◦ Good dewatering
◦ Easy to clean
Chamber
◦ Large solids capacity
◦ Good dewatering
◦ Bowl cooling possible
Disc type
◦ Solids discharge
◦ No foaming
◦ Bowl cooling possible
◦ Limited solids capacity
◦ Foams
◦ Difficult to recover
protein
◦ No solids discharge
◦ Cleaning difficult
◦ Solids recovery difficult
◦ Poor dewatering
◦ Difficult to clean
15. Bacteria
◦ Small cell size
◦ Resilient
Yeast cells
◦ Large cells
◦ Resilient
Filamentous fungi
◦ Mycelial
◦ Resilient
Cultured animal cells
◦ Large cells
◦ Very fragile
◦ High speed required
◦ Low cell damage
◦ Lower speed required
◦ Low cell damage
◦ Lower speed required
◦ High water retention in
pellet
◦ Very susceptible to
damage
16. gDu p
fp
g
2
18
rDu p
fp
c
22
18
16
The terminal velocity during gravity settling of a small
spherical particle in dilute suspension is given by Stoke’s law:
Where ug is sedimentation velocity under gravity, ρp is particle
density, ρf is liquid density, µ is liquid viscosity, Dp is
diameter of the particle, and g is gravitational acceleration.
In the centrifuge:
uc is particle velocity in the centrifuge, ω is angular velocity
in rad/s, and r is radius of the centrifuge drum.
17. 3
1
3
2
2
tan3
12
rr
g
N
2
1
2
2
2
3
2
rr
g
b
17
Disc-stack bowl centrifuge
N is number of disc, θ is half-cone angle of the disc.
The r1 and r2 are inner and outer radius of the disc, respectively.
Tubular-bowl centrifuge
b is length of the bowl, r1 and r2 are inner and outer radius of
the wall of the bowl.
18. Principle of Fermentation Technology by A.
Whitaker, P.F Stanbury, S.J. Hall.
Wikipedia.org.
Slideshare.com
NCBI.com
Hamel & Hunter- Modeling and Applications of
Downstream Processing.
Downstream processing article- springer