This project aims to functionalize carbon nanotube (CNT) films with antibodies to improve their biocompatibility for use as cell culture platforms and biomedical applications. CNT films will be prepared using layer-by-layer deposition with polyelectrolytes and functionalized with antibodies via covalent attachment or physical adsorption. The films will be characterized using Raman spectroscopy, fluorescence microscopy, and electrochemical analysis. Cell culture studies will investigate the ability of antibody-modified CNT films to support cell adhesion and proliferation. Overall, the project seeks to develop novel electrically conducting biomaterials for applications such as tissue regeneration.
Nanoparticles are solid colloidal particles ranging in size from 10 to 1000 nm.
Nanoparticles are made of a macromolecular material which can be of synthetic or natural origin.
A part of nanotechnology. Nanosensors is very hot topic for research. As nanosensor has immense applications in the fields like medical, analysis, research etc. Nanosensor recude the cost and also the time require for analysis.
Nano Material
Introduction and Synthesis
Nanomaterials describe, in principle, materials of which a single unit is sized (in at least one dimension) between 1 and 1000 nanometres (10−9 meter) but is usually 1—100 nm (the usual definition of nanoscale[1]).
Nanomaterials research takes a materials science-based approach to nanotechnology, leveraging advances in materials metrology and synthesis which have been developed in support of microfabrication research. Materials with structure at the nanoscale often have unique optical, electronic, or mechanical properties.
Nanomaterials are slowly becoming commercialized[2] and beginning to emerge as commodities.[3]
Nanoparticles are solid colloidal particles ranging in size from 10 to 1000 nm.
Nanoparticles are made of a macromolecular material which can be of synthetic or natural origin.
A part of nanotechnology. Nanosensors is very hot topic for research. As nanosensor has immense applications in the fields like medical, analysis, research etc. Nanosensor recude the cost and also the time require for analysis.
Nano Material
Introduction and Synthesis
Nanomaterials describe, in principle, materials of which a single unit is sized (in at least one dimension) between 1 and 1000 nanometres (10−9 meter) but is usually 1—100 nm (the usual definition of nanoscale[1]).
Nanomaterials research takes a materials science-based approach to nanotechnology, leveraging advances in materials metrology and synthesis which have been developed in support of microfabrication research. Materials with structure at the nanoscale often have unique optical, electronic, or mechanical properties.
Nanomaterials are slowly becoming commercialized[2] and beginning to emerge as commodities.[3]
Classification of Nanostructures by Peeyush MishraPeeyush Mishra
In this presentation, I have tried to define Nanostructures and discuss various types of Nanostructures. I have also compared the ways in which Nanomaterials can be synthesized.
Nanotechnology & nanobiotechnology by kk sahuKAUSHAL SAHU
Introduction &definition
a) Nanotechnology
b) Nanobiotechnology
History
Terms related to Nanotechnology
Nanoscale technology
Some Nanoscale related terms
What are Nanosensors
How nanosensors work
DNA Nanotechnology
How Nanotechnology works in different fields
Advantages & application of Nanotechnology
Disadvantages
Conclusion
References
Presenting a topic based on introduction to nanoscience and nanotechnology.
what is nano?
certain nomenclature like nanotechnology, nanoscience, nanomaterial, nanoscale, nanometer and so on.
surface to volume ratio and quantum effect related concepts.
future applications.
https://www.linkedin.com/in/preeti-choudhary-266414182/
https://www.instagram.com/chaudharypreeti1997/
https://www.facebook.com/profile.php?id=100013419194533
https://twitter.com/preetic27018281
Please like, share, comment and follow.
stay connected
If any query then contact:
chaudharypreeti1997@gmail.com
Thanking-You
Preeti Choudhary
Classification of Nanostructures by Peeyush MishraPeeyush Mishra
In this presentation, I have tried to define Nanostructures and discuss various types of Nanostructures. I have also compared the ways in which Nanomaterials can be synthesized.
Nanotechnology & nanobiotechnology by kk sahuKAUSHAL SAHU
Introduction &definition
a) Nanotechnology
b) Nanobiotechnology
History
Terms related to Nanotechnology
Nanoscale technology
Some Nanoscale related terms
What are Nanosensors
How nanosensors work
DNA Nanotechnology
How Nanotechnology works in different fields
Advantages & application of Nanotechnology
Disadvantages
Conclusion
References
Presenting a topic based on introduction to nanoscience and nanotechnology.
what is nano?
certain nomenclature like nanotechnology, nanoscience, nanomaterial, nanoscale, nanometer and so on.
surface to volume ratio and quantum effect related concepts.
future applications.
https://www.linkedin.com/in/preeti-choudhary-266414182/
https://www.instagram.com/chaudharypreeti1997/
https://www.facebook.com/profile.php?id=100013419194533
https://twitter.com/preetic27018281
Please like, share, comment and follow.
stay connected
If any query then contact:
chaudharypreeti1997@gmail.com
Thanking-You
Preeti Choudhary
Parte fundamental del conocimiento de Luis Fernando Heras Portillo es el saber de dónde proviene y cómo es el proceso de fabricación de su bebida favorita.
2017 Space Foundation International Student Art Contest Reflects "Space Throu...Space Foundation
Winning entries will be announced in January, and each winner receives a certificate, ribbon, an art kit and a pair of Eagle Eyes® sunglasses from the contest sponsor. Winning artwork is featured in press releases, on the Space Foundation website, on a wall display at the Space Foundation's annual Space Symposium, held each spring at The Broadmoor in Colorado Springs, Colo., and on a wall display at the Space Foundation World Headquarters and Discovery Center in Colorado Springs.
A presentation by Tshepo Kgobe (Senior Executive: Gautrain Management Agency), at the Transport Forum SIG: "Cost Effective Public Transport Management Systems" on 12 May 2016 hosted by University of Johannesburg. The theme of the presentation was: "Capacity Management in Large Projects."
Mercedes Maybach S500 S600 India launch - Press ReleaseRushLane
India’s leading luxury car brand Mercedes-Benz today launched the ultimate symbol of luxury motoring across the world, the Mercedes-Maybach in India. The pinnacle of luxury motoring was launched in India in two variants the Mercedes-Maybach S 600 and the S 500. The launch of the luxury saloon also marks the introduction of Mercedes-Benz’s new sub-brand ‘Mercedes-Maybach’ for the Indian market. The Mercedes-Maybach S 500, which is the most luxurious Mercedes-Benz ever to be locally produced in India, was rolled-out of the assembly lines by Eberhard Kern, Managing Director & CEO, Mercedes-Benz India and Piyush Arora, Executive Director, Operations, Mercedes-Benz India.
DO FASTER AND QUICKER SANDHYAAVANDANAM .
PERFORM GAYTRI JAPA WITHOUT FAIL.
IMPROVE HEALTH AND WEALTH.
MAY GOD BLESS YOU.
MAY GAYATRI SHOWER SADBUDHHI ON YOU.
Carbon nanotubes and their economic feasibilityJeffrey Funk
These slides use concepts from my (Jeff Funk) course entitled analyzing hi-tech opportunities to analyze how the economic feasibility of carbon nanotubes is becoming better through the emergence of new forms of carbon nanotubes, new methods of synthesis, and the increased scale of production equipment. New forms of carbon nanotubes continue to be developed; new ones include carbon nanobuds, doped carbon nanotubes, and graphenated carbon nanotubes, each of which includes many variations. The large number of variations suggests that carbon nanotubes will likely experience improvements in performance and the number of applications will continue to grow.
Carbon nanotubes and their economic feasibilityJeffrey Funk
These slides use concepts from my (Jeff Funk) course entitled analyzing hi-tech opportunities to analyze how the economic feasibility of carbon nanotubes is becoming better through developing new forms of carbon nanotubes, new methods of synthesis, and increasing the scale of production equipment. New forms of carbon nanotubes continue to be developed; new ones include carbon nanobuds, doped carbon nanotubes, and graphenated carbon nanotubes, each of which includes many variations. The large number of variations suggests that carbon nanotubes will likely experience improvements in performance and the number of applications will continue to grow.
Metals, however much we need it or admire it , the drawbacks of it has to be considered.( high density, susceptibility to corrosion,availability etc)
Instead planes of carbon fibre composites can be made without using a tiny scrap of metal, if only we can alter its conductivity issues. that is addressed by a research paper , on the basis of which my ppt is based.
Electrochemical behaviorof carbon paste electrode modified with Carbon Nanofi...IJERA Editor
The electrochemical behavior of carbon paste electrode modified with carbon nanofibers has been studied using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scaning electron microscopy. The response of modified electrodein ferroferricyanidesolutionshows reversible behavior and significant increment in current value compared to the bare CPE indicating that CNFs act as efficient electron mediator to catalyze reactions at the surface. The modified electrode has been used to study the electrochemical response of bisphenol Ausing different electrochemical techniques such as cyclic voltammetry, linear sweep voltammetry, differential pulse voltammetry and square wave voltammetry. The oxidation peak of BPA was observed at about 0.53 V in phosphate buffer solution at pH 6.7. The oxidation peak current of BPA varied linearly with concentration over a wide range of 5µmol L-1 to 400 µmol L-1 and the detection limit of this method was found to be 0.55 µmol L
1. Conducting surfaces can support and
enhance a range of cell lines:
• Bone re-growth
– Supronowicz et. al. J. Biomed. Mat. Res. 2002, 59, 499-506.
• Enhancing wound healing
– Zhao et. al. J. Cell Sci. 2004, 117, 397-405.
• Promoting nerve regeneration
– Schmidt et. al. P Natl. Acad. Sci. USA, 1997, 94, 8948-8953.
Materials used have included metal
electrodes, conducting polymers and
more recently carbon nanotubes
Title of the Project:
Antibody functionalization of novel electrically conducting
biomaterials to improve biocompatibility.
2. • Good conductors of heat and electricity
• Remarkable mechanical properties
• Chemically inert and thermally stable
• Modification with biomolecules can lead
to biomedical applications
• The exploration of CNTs in biomedical
applications is just underway, but has
significant potential. CNTs have been
shown to be compatible with
physiological cells and tissues
Carbon Nanotubes
3. Project aims
• Functional modification of CNTs to achieve
biocompatibility
– Functionalise with antibodies to cell adhesion molecules
– Compare covalent attachment vs. physical adsorption
• Investigate whether these antibody-modified CNT
materials are good platforms for cell culture
4. Project outline
CNT film preparation and functionalisation:
Layer by layer deposition of CNTs
Antibody functionalisation
Film characterization :
Raman and UV spectroscopy
Fluorescent microscopy and scanning
Electrochemical analysis
Cell culture studies with CNT-films:
Antibody staining and fluorescent microscopy
5. Layer by layer deposition of CNTs
13 / 14
PEL
layers
Layer by layer deposition is a technique used to attach oppositely
charged polyelectrolytes (PEL) to a surface.
It forms functional and stable films
Polyelectrolytes used include: Sodium polystyrene sulfonate (PSS-)
Poly (allylamine hydrochloride) (PAH+)
Hyaluronic acid (HA-) + CNTs
Chitosan (Chit +) + CNTs
7. Layer by layer deposition of CNTs
PAH+/PSS-/…..CNT+ Generation1 – only CNT on top layer
PSS-/PAH+/…..CNT-
PSS-/CNT+/….. Generation 2 – CNT every second layer
PAH+/CNT-/…..
CNT+/CNT-/….. Generation3 – CNT every layer
CNT-/CNT+/…..
13 / 14
PEL
layers
8. Raman Spectroscopy of CNT films
• The laser used
for the Raman
analysis was a
488nm Ar+ laser
Raman Spectroscometer
9. Raman Spectroscopy of CNT films
Wavenumber (cm-1)
RamanIntensity(a.u.)
Radial breathing
modes (RBM)
G-band
10. Antibody (Ab) immobilisation
Antibodies
• Anti-mouse FITC
• Anti- human Cy5
• Anti-mouse HRP
• Anti-Epcam
Method of immobilisation
Two methods were used:
1. Covalent attachment.
2. Physical adsorption.
11. Antibody immobilisation on CNT
films
Antibody immobilised on
CNT-/CNT+ and
CNT+/CNT-
A. Antibody physically adsorbed
to the surface.
B. Antibody attached covalently
to the surface.
A
B
12. Films soaked in
PBS for 24
hours at room
temperature
Films incubated in
cell culture media
at 37°C for 72
hours
13. After 24hrs in PBS 72hrs in
immobilisation cell culture media
Covalent immobilisation
Physical adsorption
14. Results
- Covalently attached antibody spread well over the
surface area of the film.
- CNT- PEL films are stable under physiological pH (7.0).
- CNT- PEL films are bio-compatible.
15. Fluorescent scanner studies on
CNT films
A. B. C. D.
A and B the antibody has covered the entire area.
C and D the antibody is confined to the area of attachment.
16. Electrochemical analysis of CNT
films
HRP-
Experimental setup for
electrochemical analysis
Physical adsorption
Covalent attachment
GCE GCE
17. Reduction- Fe3+ to Fe2+
Oxidation – Fe2+ to Fe3+
Glassy carbon electrode with covalently attached
antibody, could be more active electrochemically,
18. Cell culture on carbon nanotubes-
polyelectrolyte films
HeLa
cells
-Cy5
Cy5-
Cy5- goat anti-human
Anti-epcam
Anti-Mouse FITC
21. Observations and Results
- Hyaluronic acid attached to CNTs seems to be a better surface
for cell attachment and proliferation.
- Covalent attachment seems to be better surface for antibody
attachment, as the it covers more surface area of the slide.
- Physically attached antibody doesn’t cover the entire area of
the slide and was confined to the spot were it was dropped.
22. PEL/ CNT thin
films
HRP-
A
B
C
G-band
Radial breathing
modes (RBM)
D
E
A. Deposition and Characterization of CNT/PEL thin
films.
B. Biocompatibility of the thin films observed under
fluorescent microscope.
C. Functionalization of films to cell adhesion
molecules (tissue regeneration).
D. Films as biosensors.
E. Electrochemical analysis of CNT/PEL films.
Wavelength (cm-1)
Raman Spectra of
Carbon nanotubes
(CNTs)
Cyclic Voltammetry of
protein immobilized
surfaces.
Bright field and fluorescent
microscope images