Blue biotechnology utilizes marine organisms to develop or modify products. It has applications in food ingredients, algal products, biofuels, single cell protein, biosensors, and more. Transgenic fish have been created with genes for enhanced traits like growth rates, disease resistance, and altered breeding cycles. However, biotechnology also faces challenges in achieving large-scale production and raises ethical issues that require careful consideration of its applications. Overall, the document discusses the current uses and future potential of blue biotechnology while also noting areas that require more development and issues that need attention regarding its ethical implementation.
Studies on combined effect of Aeromonas hydrophila and cadmium on lipid per...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Cancer is one of the fatal disease that has no known complete cure.One of approach in developing the novel drug is through unraveling the enigma of marine biodiversity.
The early mortality syndrome (EMS) in shrimp has been ravaging production systems, spreading vertically in Asia and horizontally to countries as far away as Mexico since first reported in 2009.
Balai Perikanan Budidaya Laut Batam
Alternative strategies for minimizing the detrimental effects of bacterial infection and prevention of diseases in aquaculture are necessary since the ongoing efficacy of antibiotics is proving to be unsustainable. One of the most promising approach is the use of aqua herbal conditioners to stimulate the immune system of fish to allow them to fight off infections. In this study, the protective effect of aqua herbal conditioners produced from, mainly, mangrove and neem plant extracts in marine fish, was tested on Asian Seabass Lates calcarifer and Silver Pompano Trachinotus blochii at 8-10 g of weight size. Challenge tests were performed by immersion with two pathogenic bacteria: Vibrio harveyi and Vibrio parahaemolyticus, at a concentration of 105 cells ml-1 for 60 minutes after 12 h, 24 h and 36 h conditioning treatment. The experimental trial show that after 72 h, commercially available aqua herbal conditioners (AquaHerb) was able to significantly increase the percentage survival of L. calcarifer and T. blochii and reduces their susceptibilityto the V.harveyi and V.parahaemolyticus. Significantly higher leukocytesnumber, monocyte, neutrophil andphagocyticindexwere detected in all conditioning group for Silver Pompano and Asian Seabass. These results suggest that the combination of herbal extracts together with other trace elements contained in AquaHerb were able to act as immunostimulants and appear to improve the immune status and disease resistance of Asian Seabass and Silver Pompano.
Many Gram negative bacteria, especially Aeromonas hydrophila are notorious for their heightened capacity to acquire and exchange antibiotic resistance genes and consequently, are commonly targeted as indicator organism for monitoring antimicrobial resistance in aquatic milieus. This study was aimed to investigate the prevalence and drug resistance patterns of Aeromonas hydrophila isolated from farm raised catfish at Epe fish farm, Lagos State, Nigeria. Swabs were aseptically taken from the kidney, intestine, liver, gills, and skin after dissecting the fish samples. The specimens were bacteriologically analyzed. After series of biochemical test, the isolated bacteria were identified presumptively according to Bergey’s Manual of determinative bacteriology, 7th Edition. Fifty-seven (57) Aeromonas hydrophila were recovered out of seventy-one (71) bacterial isolated from the 35 fish samples collected from the fish farms studied. The study reveals multiple antibiotics resistance pattern among the isolates as Aeromonas hydrophila were 100% resistant to Streptomycin, Oxytetracycline, Chloranphenicol, Pefloxacin, Ofloxacin and 70, 65 and 55% resistant to Gentamycin, Amoxycillin and Erythromycin respectively. However, all the isolates were 100% susceptible to Ciprofloxacin and Cotrimoxazole only. The presence of multidrug-resistant Aeromonas hydrophila in fish could be a vehicle of horizontal gene transfer to previously susceptible bacteria and these could constitute a serious public health hazard to human and animal in the environment. Thus, the use of antibiotics in catfish production as growth promoter or disease prevention should be discouraged and some safer, biological alternatives strategies should suffice to mitigate bacteria drug resistance and its associated problems.
Isolation of a Gram Negative Fish Pathogenfrom Moroccan Rainbow Trout Hatcher...IJAEMSJORNAL
Salmonidaquaculture represents one of the most important fish groups in the aquaculture industry (FAO, 2016). However, the success and sustainability of salmonid aquaculture largely depend on disease control. Although Rainbow trout is relatively sensitive to diseases, several bacterial, viral and parasitic diseases have been reported. However, only opportunistic pathogens whose infectivity is expressed by a decline in the fish conditions and of its natural defenses, linked to disturbances of the environment or livestock practices. Livestock is the most favorable context for the development of bacterial pathologies, that’s why we have too many bacterial pathogenic species for fish; the most serious infections in our latitudes are Aeromonadaceae, Vibrionaceae, Enterobacteriaceae and Flavobacteriaceae (Noga 1996, Austin and Austin 1999, Woo and Bruno 1999). Moroccan aquaculture has undergone a rapid development and expansion over the last decade, to this end, Rainbow trout (OnchorhyncusMykiss) have been maintained at AinAghbal Fish farm – Azrou-Morocco; for production, livestock and transformation activity and also for commercialization. The most important bacterial freshwater pathogen affecting salmonid livestock in fish farms in Morocco is the cold-water disease as a result of affection by Flavobactriumpsychrophilum bacteria. Our study aimed on approving the presence of this bacterium and to characterize it biochemically, enzymatically and also physiologically.
Studies on combined effect of Aeromonas hydrophila and cadmium on lipid per...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Cancer is one of the fatal disease that has no known complete cure.One of approach in developing the novel drug is through unraveling the enigma of marine biodiversity.
The early mortality syndrome (EMS) in shrimp has been ravaging production systems, spreading vertically in Asia and horizontally to countries as far away as Mexico since first reported in 2009.
Balai Perikanan Budidaya Laut Batam
Alternative strategies for minimizing the detrimental effects of bacterial infection and prevention of diseases in aquaculture are necessary since the ongoing efficacy of antibiotics is proving to be unsustainable. One of the most promising approach is the use of aqua herbal conditioners to stimulate the immune system of fish to allow them to fight off infections. In this study, the protective effect of aqua herbal conditioners produced from, mainly, mangrove and neem plant extracts in marine fish, was tested on Asian Seabass Lates calcarifer and Silver Pompano Trachinotus blochii at 8-10 g of weight size. Challenge tests were performed by immersion with two pathogenic bacteria: Vibrio harveyi and Vibrio parahaemolyticus, at a concentration of 105 cells ml-1 for 60 minutes after 12 h, 24 h and 36 h conditioning treatment. The experimental trial show that after 72 h, commercially available aqua herbal conditioners (AquaHerb) was able to significantly increase the percentage survival of L. calcarifer and T. blochii and reduces their susceptibilityto the V.harveyi and V.parahaemolyticus. Significantly higher leukocytesnumber, monocyte, neutrophil andphagocyticindexwere detected in all conditioning group for Silver Pompano and Asian Seabass. These results suggest that the combination of herbal extracts together with other trace elements contained in AquaHerb were able to act as immunostimulants and appear to improve the immune status and disease resistance of Asian Seabass and Silver Pompano.
Many Gram negative bacteria, especially Aeromonas hydrophila are notorious for their heightened capacity to acquire and exchange antibiotic resistance genes and consequently, are commonly targeted as indicator organism for monitoring antimicrobial resistance in aquatic milieus. This study was aimed to investigate the prevalence and drug resistance patterns of Aeromonas hydrophila isolated from farm raised catfish at Epe fish farm, Lagos State, Nigeria. Swabs were aseptically taken from the kidney, intestine, liver, gills, and skin after dissecting the fish samples. The specimens were bacteriologically analyzed. After series of biochemical test, the isolated bacteria were identified presumptively according to Bergey’s Manual of determinative bacteriology, 7th Edition. Fifty-seven (57) Aeromonas hydrophila were recovered out of seventy-one (71) bacterial isolated from the 35 fish samples collected from the fish farms studied. The study reveals multiple antibiotics resistance pattern among the isolates as Aeromonas hydrophila were 100% resistant to Streptomycin, Oxytetracycline, Chloranphenicol, Pefloxacin, Ofloxacin and 70, 65 and 55% resistant to Gentamycin, Amoxycillin and Erythromycin respectively. However, all the isolates were 100% susceptible to Ciprofloxacin and Cotrimoxazole only. The presence of multidrug-resistant Aeromonas hydrophila in fish could be a vehicle of horizontal gene transfer to previously susceptible bacteria and these could constitute a serious public health hazard to human and animal in the environment. Thus, the use of antibiotics in catfish production as growth promoter or disease prevention should be discouraged and some safer, biological alternatives strategies should suffice to mitigate bacteria drug resistance and its associated problems.
Isolation of a Gram Negative Fish Pathogenfrom Moroccan Rainbow Trout Hatcher...IJAEMSJORNAL
Salmonidaquaculture represents one of the most important fish groups in the aquaculture industry (FAO, 2016). However, the success and sustainability of salmonid aquaculture largely depend on disease control. Although Rainbow trout is relatively sensitive to diseases, several bacterial, viral and parasitic diseases have been reported. However, only opportunistic pathogens whose infectivity is expressed by a decline in the fish conditions and of its natural defenses, linked to disturbances of the environment or livestock practices. Livestock is the most favorable context for the development of bacterial pathologies, that’s why we have too many bacterial pathogenic species for fish; the most serious infections in our latitudes are Aeromonadaceae, Vibrionaceae, Enterobacteriaceae and Flavobacteriaceae (Noga 1996, Austin and Austin 1999, Woo and Bruno 1999). Moroccan aquaculture has undergone a rapid development and expansion over the last decade, to this end, Rainbow trout (OnchorhyncusMykiss) have been maintained at AinAghbal Fish farm – Azrou-Morocco; for production, livestock and transformation activity and also for commercialization. The most important bacterial freshwater pathogen affecting salmonid livestock in fish farms in Morocco is the cold-water disease as a result of affection by Flavobactriumpsychrophilum bacteria. Our study aimed on approving the presence of this bacterium and to characterize it biochemically, enzymatically and also physiologically.
Canvax™ offers a wide range of high quality Human Recombinant Proteins for several research applications like ELISA, Western Blot, Antibody Production or Protein array.
Benefits of forage systems on product quality: mechanisms and implications - ...Sustainable Food Trust
Kevin J Shingfield's presentation from the Sustainable Food Trust's meeting: What role for grazing livestock in a world of climate change and diet-related disease?
Bryophyllum Pinnatum: A Potential Attenuator of Cadmium-Induced Oxidative Str...IOSR Journals
Cadmium has been famously implicated in the stimulation of free radical production in biosystems resulting in oxidative deterioration of lipids, proteins and DNA, and initiating various pathological conditions in humans and animals. This study therefore, examined the antidotal and ameliorative capacity of crude ethanolic extract of Bryophyllum pinnatum on cadmium-induced oxidative stress using rabbit models. A total of fifteen rabbits (1.30±0.05kg) were used for the study. After two weeks of acclimatization, the rabbits were randomly rifted into three experimental groups- (N, CD & CB) with five animals per group. The control group (N) was injected normal saline intraperitoneally (3mg/kg body weight) and the test groups (CD & CB) were administered cadmium once daily by subcutaneous injection (3mg/kg body weight). The ethanolic extract of the plant was orally administered once daily at a dose of 100mg/kg body weight. The oxidative and antioxidative stress parameters were assessed in tissues. The results showed significant difference (p˂ 0.05)in treated groups relative to the control group with the exception of glutathione peroxidase activity in leg muscles. Therefore, the results obtained in this study confirmed the potency of the plant to annihilate cadmium toxicity in animals
Cloning and Expression of Outer Membrane Protein Omp38 Derived from Aeromonas hydrophila in Escherichia coli
http://dx.doi.org/10.21276/SSR-IIJLS.2019.5.3.8
Effect of nitrogen and phosphorus amendment on the yield of a Chlorella sp. s...Agriculture Journal IJOEAR
Abstract— A strain of microalgae was isolated from phytoplankton samples collected from the sea coast of Amsheet, North Lebanon. Molecular diagnosis based on ribosomal RNA genes showed it to be most closely related to Chlorella sp. (GenBank accession KC188335.1) with over 90 % nucleotide identity. It was then evaluated whether N and P amendments of seawater fertilized with Guillard’s f/2 medium would improve algal growth and production. Addition of nitrogen (30 ppm) and/or phosphorus (2 ppm) to microalgae grown under laboratory conditions in 3L bioreactors resulted in improved biomass yield (mg dry matter/ L) by approximately 48%, and increased protein yield by approximately 56%, from 19.5% to 30.6% of DM content. Total protein yield/L of culture medium was therefore increased by approximately 83%. Total lipid content and carotenoid levels of the microalgal culture were not affected by the N+P amendement, whereas chlorophyll content was almost doubled. When lower levels of N+P supplementations, 10 and 20 ppm N, were tried, the biomass yield was also improved. The experiment was repeated in 20 L bioreactors in a plastic greenhouse, under normal environmental conditions, with an average temperature of 28°C and a maximum temperature of 36°C. At these relatively high temperatures, the growth rate was slowed down, but N supplementations at 10 and 20 ppm resulted in improved dry matter yield by 25 and 45% respectively, and protein content by 17 and 35%, respectively. Knowledge of the optimal culturing conditions of this local Chlorella strain is essential for its efficient production and is expected to serve future environmental and biotechnological purposes.
Growth Pattern, Molecular Identification and Bio molecules Analysis of FOMITO...journal ijrtem
Abstract : Fomitopsis feei, a brown rot fungus is identified tentatively using morphological characteristics and confirmed phylogenetically by 28S rDNA analysis and sequence was submitted in EMBL Nucleotide Sequence Database. Its growth pattern was studied on eight different solid media and found to be good on Malt extract agar medium. Biomolecules such as proteins and lipid were screened qualitatively and estimated quantitatively. Aminoacid analysis by chromatography and fatty acid analysis by FAME were also done and revealed that tryptophan (20.53%), valine (20.51%) and cis-linoleic acid (43.38%) and palmetic acid (17.88%) were in high percentage.
Key words : Fomitopsis feei, growth, molecular identification and biomolecules
Management of anthelmintic resistance.pptxssuser27dccd
Drug resistance is the most severe barrier in controlling many parasitic species that infect animals and humans. Resistance to anthelmintic drugs amongst the major nematode parasites has reached alarming proportions worldwide and threatens the future viability of continued animal production in many countries. Effective control and management strategies using anthelmintics require a precise appraisal of the resistance or susceptibility of the target worms.
Feed ingredients and feed prices are increasing; it is becoming harder to maintain the nutritional balance of the feed without increasing too much the feed price. Now, the use of ingredients from less stringent quality is likely to increase. Though plant materials are usually more reasonable in price than animal products, they can present problems through the presence of naturally occurring contaminants. Indeed, contamination of feed commodities by microorganisms and mycotoxins is the first negative factor impacting animal feed quality. Numerous researches have studied the decrease of performances with contaminated feeds.
The nutritional composition and fungal spoilage of Dacryodes edulis fruits were carried out in the Department of Forestry/ Environment Laboratory using standard procedures. The experiment was laid out in a Completely Randomized Design (CRD) with six treatments and three replicates. The fungal pathogens isolated from the rotted fruits were Aspergillus niger, Aspergillus flavus, Rhizopus stolonifer, Fusarium pallidoroseum, Botryodiplodia theobromae and Colletotrichum gloeosporioides. The predominant spoilage causing fungi were Colletotrichum gloeosporioides (60%) and Aspergillus niger (52%). Proximate analysis revealed that the affected fruits had significantly reduced (P<0.05) quality when compared to the uninfected fruits in terms of carbohydrate content, protein, oil content, moisture, crude fibre and Ash content. This work holds promise on the importance of the nutritional properties of the fruits in screening for rot tolerance and storage stability.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptxMAGOTI ERNEST
Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
ISI 2024: Application Form (Extended), Exam Date (Out), EligibilitySciAstra
The Indian Statistical Institute (ISI) has extended its application deadline for 2024 admissions to April 2. Known for its excellence in statistics and related fields, ISI offers a range of programs from Bachelor's to Junior Research Fellowships. The admission test is scheduled for May 12, 2024. Eligibility varies by program, generally requiring a background in Mathematics and English for undergraduate courses and specific degrees for postgraduate and research positions. Application fees are ₹1500 for male general category applicants and ₹1000 for females. Applications are open to Indian and OCI candidates.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Phenomics assisted breeding in crop improvementIshaGoswami9
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
3. 3Branches of biotechnology
• Blue biotechnology
• Marine organism
• White
biotechnology
• Industry
• Red biotechnology
Medicine
• Green
biotechnology
• Agriculture
4. Outline
Introduction
Role of blue biotechnology
Applications
Transgenic fishes
Summary
Future line of work
4
21. …single cell protein
Central Food Technological Research Institute, Mysore (CFTRI)
National Botanical Research Institute, Lucknow (NBRI)
21
Composition of groups by % dry weight Spirulina tablet
24. 24
A Pichia pastoris clone - shrimp alkaline phosphatase gene from
Northern shrimp Pandalus borealis
Completely and irreversibly inactivated by heating at 65°C for 5
minutes, thereby making removal of rSAP prior to ligation or end-
labeling unnecessary
Pichia pastoris SAP
Recombinant single alkaline phosphatases
33. Classes of fish
Genetic engineering techniques
To introduce a new trait to the fish which does not
occur naturally in the species
33
• Agnatha
(jawless fish)
• Chondrichythes
(cartilaginous fish)
• Osteichythes
(bony fish)
34. Why GM Fish?
FAO – 2025 - seven-fold more aquaculture products
Blue Revolution - technology from the biological sciences and
engineering
Low cost production independent of proximity to the oceans
Species - Less invasive to the environment
Increased growth rates
Enhanced resistance to disease
Alteration of breeding cycles
34
(Taylor and Francis, 2009)
35. GM fishes-covers four broad areas
GM
Enhance
trait
Bioreactors
Pollution
indicators
Genomic
studies
36
37. …expression of antifreeze proteins in transgenic
plants
Synthetic Anti freeze
gene
afa3 gene
Not effective
Chimeric antifreeze gene
Staphylococus protein A X afa5
Spa-afa5
Recrystallization inhibition
37
CaMV-promoter
Agrobacterium co culture
(Hightower et al., 1991)
38. AquAdvantage salmon
AquaBounty Technologies
US FDA - production, sale and consumption
First genetically modified animal to be approved for
human consumption
38Transgenic Salmon
41. GloFish
Patented and trademarked brand
Sold in US only
Variety
Not meant for consumption
$50 – 1/10 of normal zebra fish
41
GloFish
42. 42
Green fluorescent protein (GFP) - Aequora victoria (jellyfish)
Caenorhabditus elegans-first
All kingdoms – GFP
ANDi (Chan, A.W.S et al)
GloFish
Fluorescent Drossophila
Fluorescent ANDi
(Stewart, 2006)
...GloFish
43. Fluorescence pattern
Nicotiana tabacum cv. Xanthi
mGFP5er gene
Promotor - CaMV 35S
Vascular & young leaves
Adaxial close to stem
43
(Harper and Stewart, 2000)
Whole plant fluorescence pattern
44. ICAR-CMFRI
Patented products and techniques
44
Shore pearl
culture technology
Green mussels
extract (GMe)
for arthritis
Hatchery
technology for
clown fish
45. Summary
Blue biotechnology – novel or modified products – by
growing/manipulating organisms
Raising population
Food, algal products, single cell protein, cancer applications,
biosensors
Transgenic fishes
Role of GFP
45
47. …work-yet to be done!
Large supply-challenging
Advanced technology
GM-borderline on ethics
47
48. 48
Biotechnology and its products have created some
amazing possibility but also has raised fears. Hence,
we should utilize biotechnology wisely & for a good
cause
Sea food & health supplements
Cancer, neural diseases, skin diseases
Red-medical applications, to produce drugs, antibiotics from marine natural products
white/industrial biotechnology- in markets-food and feed, detergents, paper and pulp, textiles and bioenergy (such as biofuels or biogas)
Spirullina, dunaliella, chlorella, laminaria, porphyra-nutraceuticals, vit A, antioxidant property
Extremophiles, bacteria, cyanobacteria, algae, yeast-enzymes
Sponges-high lipid content-fatty acids, sterols, and unsaponifiable compunds as compare to higher animals. natural abioactive compounds - therapeutics
By-product, PUFA-protamines-act as antibacterial in food industry
Prawn waste and shellfish waste-single cell protein by marine algae of candida sp.
Carotenoids –phycobilin, phycocyanin, b carotene-maicroalgae chlorella -precursor of vit. A
Lipid – omega 3 -sardine, salmon, tuna-cardiovascular
Polysachhardide-Algine from laminaria brown, carrageenan-red algae-stabalizers
Enzyme-gastric protease, lipase, phenol oxidase from crustaceans– numerous benefits-cardiovascular
Collagen, gelatiin, albumin, protamine – fish spermatozoa-natural preservative
Red algae-Porphyra (nori)-food source in Japan for 400 years. Vit. A, C, E, B1,B2, B6, B12. microalgal oil food supplemets in baby food
Can remove Cu, Cr, Ni, Zn. Lead from 1-1000ppm
World population-9 billion-2050
Body weight, lowers blood pressure, healthy eyes and skin. B carotene- anti cancer agents
Electric Green", "Sunburst Orange", "Moonrise Pink", "Starfire Red", "Cosmic Blue", and "Galactic Purple"