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By Nadeem Khalid
2017-dvmn-010
1
Table of Contents
Introduction
What blood transfusion is?
Cross Matching
Chemicals, Procedure & Interpretation
Indications
Under what conditions
Blood transfusion is
recommended?
Collection
Sites & Rate of Collection and
Storage of Blood
Administration
Volume, Route & Rate
Complications
Unexpected conditions
2
Blood transfusion is the
transfusion of whole blood or its
Component such as blood cells or
plasma from one individual to
another.
Introduction
Definition
3
Blood Groups
13
03
13
09
4
Indications
★ Acute Haemorrhage
★ Chronic Haemorrhage
★ Haemolytic Anemia
★ Thrombocytopenia
★ Coagulopathies
★ Undernutrition
★ Parasitism
★ Heat Injury
★ Chemical Burns
★ Electrical Burns
Bleeding Disorders Hypoproteinemia Burns
Anaemia
5
Hb
< 7 g/dl
PCV
< 20% P Protein
< 3.5 g/dl
Criteria for BT
6
Haematology
Some Normal Parameter
Specie Hb(g/dl) PCV(%) Plasma protein(g/dl)
Dog 13 45.5 5.5-8
Cat 12 40 6-8
Cattle Cow:11.3
Buffalo: 12.9
Cow: 33.7
Buffalo: 44.3
6.7-7.5
Horse 11.5 39.4
Sheep 14.4,(Goat 10.9) 32,(Goat 34) 6-7.9, Goat (6.4-7)
7
Selection of Donor
Donor Selection
Character Remark
Specie Same Specie and Breed
Health Status Apparently Healthy and free from Infectious Disease
Major Test Dogs: Brucella Canis, Heartworm
Cat: FeLV, Hemobartonella
Ruminants: Blood parasite test
PCV Dog: >40%, Cat: >35%
Hb Dog: >13g/dl, Cat: >11g/dl
8
● Importance
○ Done to prevent Life threatening Antigen antibody reactions caused by major
blood group Mismatch
● Usefulness
○ Not often use practice in animals except Horse and Dogs
○ In Cattle first transfusion can be done without cross matching however, it is
essential if the recipient is given more than one transfusion
Blood Compatibility
9
Direct
Rapid Method
Indirect
Most Reliable Oehlecker’s
Oehlecker’s
Biological Method
3 Methods
10
Direct Method
Donor blood = 1 ml
Normal Saline = 3m
Mixing to form
Solution
Glass Slide
Solution = 2 drops
Recipient Serum= 2 drops
Agitation
3-5 Minutes
Agglutination indicate
incompatibility
Step 1 Step 2 Step 3 Step 4
11
Indirect Method
Recipient
Donar
Sample 1 Sample 2 Sample 2 Sample 1
EDTA, Vacutainer
Yellow cap
Vacutainer
Yellow cap
Vacutainer
EDTA, Vacutainer
Centrifuge,
separate RBC
Serum Serum
Centrifuge,
separate RBC
Keep in 3 ml of
0.85% Saline
Solution
Keep in 3 ml of
0.85% Saline
Solution
12
Indirect Method
Transfuse Blood only if both system Show
Compatibility
13
Oehlecker’s Method
Step 1
Inject 10 ml blood
to Recipient
Step 2
Observe for 20
Minutes
Step3
If No Untoward Reaction,
then Transfuse remaining
blood
Most Suitable under Field Condition
14
Collection of Blood
Sites of Collection
Species Sites
Dog Saphenous, Cephalic or Jugular Vein
Cat Saphenous, Cephalic or Jugular Vein
Horse Jugular vein
Cattle Jugular vein, Caudal(Tail) vein or Mammary vein
Sheep/Goat Jugular vein or cephalic vein
15
Steps
1. Restraining and Sedation
2. Shaving and Vein Prominent
3. Sterilization
4. Insert Needle (16-18G)
5. Mixing with preservative
Collection of Blood
Link: https://drive.google.com/file/d/1194fSCEvXsvR_Yq9j7tj8wvcDELf_jsB/view?resourcekey
16
Dog
● 20 ml blood/kg body weight
● Maximum 450-500ml
Cat
● 10 ml blood/kg body weight
● Maximum 50ml
● 12% of B.W is blood
● 20% of blood can be donated
● 10-15 ml blood/kg B.W
Large Animals
Volume to be Donated
17
Different Preservatives
1. Acid Citrate Dextrose (ACD),
2. Citrate Phosphate Dextrose, Adenine (CPD-A)
3. Citrate 3.8% (1o ml for 100 ml blood)
4. Heparin 15 IU/ ml
Collection and Storage
18
Collection and Storage
ACD Solution
1. Dextrose
2. Tri-sodium Citrate
3. Citric Acid
4. Distilled Water
CPD-A Solution
1. Dextrose
2. Sodium Citrate(Dehydrate)
3. Citric Acid (Anhydrous)
4. Sodium Phosphate(Monohydrate)
5. Adenine (Anhydrous)
6. Water
It is used @ 15 ml/100 ml blood It is used @ 15 ml/100 ml blood
19
● Room Temperature
○ Used within 4-8 hours of collection
● 4 C
○ At Least 3 weeks possibly as long as 6 weeks when
Collected in ACD, CPD, CPD-A
Storage
❏ Adequate levels of 2-3 Diphosphoglycerate DPG are to be Maintained in stored blood
❏ DPG helps to release O2 to RBC cells and other tissues
20
Direct
● Autotransfusion
● Actual quantity to be transfused
can not be determined
Indirect
● Most Commonly used
● Here stored blood is used
Administration
21
Routes
● Intravenous: Emergency Situation
● Intraperitoneal: Animal in incorporative
○ 50% absorb in 24 hours
○ Remaining absorb within week
Rate
● Rate depend upon
○ Hydration status
○ Degree of Anemia
● Normovolemic: 10-20 ml/kg/hr
● Hypovolemic: 20-60 ml/kg/hr
● Cardiac Failure: 4 ml/kg/hr
Route & Rate
22
Generally:
10-20 ml/kg Body weight
Dogs and Cats:
Volume
Blood (ml)= B.W * K* ( Desired PCV-Recipient PCV)/Donar PCV
K for, Dogs: 80, Cats: 60
23
Canine Transfusion Calculator
Link: https://www.petbloodbankuk.org/vet-professionals/i-need-advice/canine-transfusion-calculator/
1 2
3
24
● Steps
○ Prewarming before 30 minutes ago upto 22-37 C
○ Inject Steroid or Antihistamine to recipient before Transfusion
○ Proper placement of Branula
○ Blood bag connected to BT set and immersed in warm water
○ In-line nylon mesh micro filter
○ No concurrent administration of drug or hypertonic solution
○ Manage Route and rate accordingly
○ Transfuse proper Calculated Volume
Administration of Blood
25
Complications
Non- Immunogenic
1. Fever
2. Vomition
3. Hemolysis
4. Transmission of infection
5. Congestive Heart Failure
6. Citrate Toxicity
7. Coagulopathy
Immunogenic
1. Anaphylaxis
2. Hemolysis
3. DIC, Renal Failure
4. Urticaria
5. Hypothermia
Others
1. Hypocalcemia
2. Carcinoma
3. Rapid Recurrence of
Anemia
26
● Monitor the Recipient Continuously
● Stop Blood Transfusion if any reaction Occur
● Mild Reaction:
○ Antihistamines and Corticosteroids (Decadron @ 0.1-0.5mg/kg )
● Severe Reaction:
○ Adrenaline 0.01-0.02ml/kg
● Citrate Toxicity:
○ Calcium Preparation IV @ 10 ml /liter of blood
● Congestive Heart Failure
○ Diuretic
Therapeutic Management
27
1. Careful Selection of healthy Donor
2. Perform cross matching prior to transfusion
3. Warm the stored blood to body temperature before transfusion
4. Filter the blood through sterile gauze if froth is formed
5. Give antihistamine before transfusion
Precaution
28
1. Textbook of Clinical Veterinary Medicine by Amalendu Chakrabarti
2. VETERINARY Medicine, A textbook of the diseases of cattle, horses, sheep, pigs and goats 9th EDITION O. M.
Radostits
References
29
— THANKS
“Until one has loved an animal,
a part of one's soul remains
unawakened.”
30

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Blood Transfusion in Animals.pdf

  • 2. Table of Contents Introduction What blood transfusion is? Cross Matching Chemicals, Procedure & Interpretation Indications Under what conditions Blood transfusion is recommended? Collection Sites & Rate of Collection and Storage of Blood Administration Volume, Route & Rate Complications Unexpected conditions 2
  • 3. Blood transfusion is the transfusion of whole blood or its Component such as blood cells or plasma from one individual to another. Introduction Definition 3
  • 5. Indications ★ Acute Haemorrhage ★ Chronic Haemorrhage ★ Haemolytic Anemia ★ Thrombocytopenia ★ Coagulopathies ★ Undernutrition ★ Parasitism ★ Heat Injury ★ Chemical Burns ★ Electrical Burns Bleeding Disorders Hypoproteinemia Burns Anaemia 5
  • 6. Hb < 7 g/dl PCV < 20% P Protein < 3.5 g/dl Criteria for BT 6
  • 7. Haematology Some Normal Parameter Specie Hb(g/dl) PCV(%) Plasma protein(g/dl) Dog 13 45.5 5.5-8 Cat 12 40 6-8 Cattle Cow:11.3 Buffalo: 12.9 Cow: 33.7 Buffalo: 44.3 6.7-7.5 Horse 11.5 39.4 Sheep 14.4,(Goat 10.9) 32,(Goat 34) 6-7.9, Goat (6.4-7) 7
  • 8. Selection of Donor Donor Selection Character Remark Specie Same Specie and Breed Health Status Apparently Healthy and free from Infectious Disease Major Test Dogs: Brucella Canis, Heartworm Cat: FeLV, Hemobartonella Ruminants: Blood parasite test PCV Dog: >40%, Cat: >35% Hb Dog: >13g/dl, Cat: >11g/dl 8
  • 9. ● Importance ○ Done to prevent Life threatening Antigen antibody reactions caused by major blood group Mismatch ● Usefulness ○ Not often use practice in animals except Horse and Dogs ○ In Cattle first transfusion can be done without cross matching however, it is essential if the recipient is given more than one transfusion Blood Compatibility 9
  • 10. Direct Rapid Method Indirect Most Reliable Oehlecker’s Oehlecker’s Biological Method 3 Methods 10
  • 11. Direct Method Donor blood = 1 ml Normal Saline = 3m Mixing to form Solution Glass Slide Solution = 2 drops Recipient Serum= 2 drops Agitation 3-5 Minutes Agglutination indicate incompatibility Step 1 Step 2 Step 3 Step 4 11
  • 12. Indirect Method Recipient Donar Sample 1 Sample 2 Sample 2 Sample 1 EDTA, Vacutainer Yellow cap Vacutainer Yellow cap Vacutainer EDTA, Vacutainer Centrifuge, separate RBC Serum Serum Centrifuge, separate RBC Keep in 3 ml of 0.85% Saline Solution Keep in 3 ml of 0.85% Saline Solution 12
  • 13. Indirect Method Transfuse Blood only if both system Show Compatibility 13
  • 14. Oehlecker’s Method Step 1 Inject 10 ml blood to Recipient Step 2 Observe for 20 Minutes Step3 If No Untoward Reaction, then Transfuse remaining blood Most Suitable under Field Condition 14
  • 15. Collection of Blood Sites of Collection Species Sites Dog Saphenous, Cephalic or Jugular Vein Cat Saphenous, Cephalic or Jugular Vein Horse Jugular vein Cattle Jugular vein, Caudal(Tail) vein or Mammary vein Sheep/Goat Jugular vein or cephalic vein 15
  • 16. Steps 1. Restraining and Sedation 2. Shaving and Vein Prominent 3. Sterilization 4. Insert Needle (16-18G) 5. Mixing with preservative Collection of Blood Link: https://drive.google.com/file/d/1194fSCEvXsvR_Yq9j7tj8wvcDELf_jsB/view?resourcekey 16
  • 17. Dog ● 20 ml blood/kg body weight ● Maximum 450-500ml Cat ● 10 ml blood/kg body weight ● Maximum 50ml ● 12% of B.W is blood ● 20% of blood can be donated ● 10-15 ml blood/kg B.W Large Animals Volume to be Donated 17
  • 18. Different Preservatives 1. Acid Citrate Dextrose (ACD), 2. Citrate Phosphate Dextrose, Adenine (CPD-A) 3. Citrate 3.8% (1o ml for 100 ml blood) 4. Heparin 15 IU/ ml Collection and Storage 18
  • 19. Collection and Storage ACD Solution 1. Dextrose 2. Tri-sodium Citrate 3. Citric Acid 4. Distilled Water CPD-A Solution 1. Dextrose 2. Sodium Citrate(Dehydrate) 3. Citric Acid (Anhydrous) 4. Sodium Phosphate(Monohydrate) 5. Adenine (Anhydrous) 6. Water It is used @ 15 ml/100 ml blood It is used @ 15 ml/100 ml blood 19
  • 20. ● Room Temperature ○ Used within 4-8 hours of collection ● 4 C ○ At Least 3 weeks possibly as long as 6 weeks when Collected in ACD, CPD, CPD-A Storage ❏ Adequate levels of 2-3 Diphosphoglycerate DPG are to be Maintained in stored blood ❏ DPG helps to release O2 to RBC cells and other tissues 20
  • 21. Direct ● Autotransfusion ● Actual quantity to be transfused can not be determined Indirect ● Most Commonly used ● Here stored blood is used Administration 21
  • 22. Routes ● Intravenous: Emergency Situation ● Intraperitoneal: Animal in incorporative ○ 50% absorb in 24 hours ○ Remaining absorb within week Rate ● Rate depend upon ○ Hydration status ○ Degree of Anemia ● Normovolemic: 10-20 ml/kg/hr ● Hypovolemic: 20-60 ml/kg/hr ● Cardiac Failure: 4 ml/kg/hr Route & Rate 22
  • 23. Generally: 10-20 ml/kg Body weight Dogs and Cats: Volume Blood (ml)= B.W * K* ( Desired PCV-Recipient PCV)/Donar PCV K for, Dogs: 80, Cats: 60 23
  • 24. Canine Transfusion Calculator Link: https://www.petbloodbankuk.org/vet-professionals/i-need-advice/canine-transfusion-calculator/ 1 2 3 24
  • 25. ● Steps ○ Prewarming before 30 minutes ago upto 22-37 C ○ Inject Steroid or Antihistamine to recipient before Transfusion ○ Proper placement of Branula ○ Blood bag connected to BT set and immersed in warm water ○ In-line nylon mesh micro filter ○ No concurrent administration of drug or hypertonic solution ○ Manage Route and rate accordingly ○ Transfuse proper Calculated Volume Administration of Blood 25
  • 26. Complications Non- Immunogenic 1. Fever 2. Vomition 3. Hemolysis 4. Transmission of infection 5. Congestive Heart Failure 6. Citrate Toxicity 7. Coagulopathy Immunogenic 1. Anaphylaxis 2. Hemolysis 3. DIC, Renal Failure 4. Urticaria 5. Hypothermia Others 1. Hypocalcemia 2. Carcinoma 3. Rapid Recurrence of Anemia 26
  • 27. ● Monitor the Recipient Continuously ● Stop Blood Transfusion if any reaction Occur ● Mild Reaction: ○ Antihistamines and Corticosteroids (Decadron @ 0.1-0.5mg/kg ) ● Severe Reaction: ○ Adrenaline 0.01-0.02ml/kg ● Citrate Toxicity: ○ Calcium Preparation IV @ 10 ml /liter of blood ● Congestive Heart Failure ○ Diuretic Therapeutic Management 27
  • 28. 1. Careful Selection of healthy Donor 2. Perform cross matching prior to transfusion 3. Warm the stored blood to body temperature before transfusion 4. Filter the blood through sterile gauze if froth is formed 5. Give antihistamine before transfusion Precaution 28
  • 29. 1. Textbook of Clinical Veterinary Medicine by Amalendu Chakrabarti 2. VETERINARY Medicine, A textbook of the diseases of cattle, horses, sheep, pigs and goats 9th EDITION O. M. Radostits References 29
  • 30. — THANKS “Until one has loved an animal, a part of one's soul remains unawakened.” 30