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Noha M. El Husseiny, MD 
Clinical Haematology Unit 
Cairo University
Red blood cell transfusion 
Whole blood 
450ml contains adequate coagulation 
factors but less platelets. 
Rarely used nowadays.
Fresh Frozen Plasma 
Preparation from whole blood separated and 
frozen within 8 hours can be kept up to one 
year. 
Volume 200 ml blood 
To increase factor by 20%need 10-20 ml/kg 
Properties Expire in 24h after thawing 
however thawed plasma can be relabelled and 
used within five days in TTP.
Cryoprecipitate
Cryoprecipitate 
The insoluble part of plasma when thawed 
to 4 C 
10-20 ml 
Properties rich in factor VIII, fibrinogen, 
factor XIII and VWF (variable concentrate) 
Correction of hypofibrinogenemia (need 8- 
10 units) if fibrinogen 50-100mg/ L
Factor concentrates 
Recombinant factor VIII 
Recombinant factor VII 
Factor IX concentrates 
VWF concentrate
Packed red blood cell 
Preparation are separated from whole 
blood by centrifugation. Unit is 200ml. 
Increase HB 1-2 g/ml 
Indications: transfusion if HB less than 
6g/dl 
Stored up to 6 weeks.
Warming of blood components
Leuko-reduced packed RBCs 
Contain less than 107 leukocytes per 
unit. 
Used to reduce CMV infection, HLA 
immunization, Febrile reaction and 
platelet transfusion refractoriness.
Leukoreduced gamma irradiated PRBCs For 
patients in transplants 
Washed RBCs using saline to reduce allergic 
reaction. 
Frozen RBCs: Add glycerol and freeze RBCs 
within 6 days of collection used in preserving 
rare groups for long time than 42 days.
Platelet preparation
Platelet transfusion 
Preparation may be separated from 
whole blood or collected by apharesis. 
Stable for 72 H 
Indications: Thrombocytopenia. 
Contraindications TTP, HIT
Immunoglobulins 
Preparation by cold ethanol fractionation 
of large pool of plasma followed by viral 
inactivation. 
Uses treatment of ITP, Guillian Barre 
syndrome, chronic inflammatory 
demyelinating polyneuropathy, 
replacement in immunodeficiency 
syndrome.
Blood 
Group 
Antigens Antibodi 
es 
Can give 
blood to 
Can 
receive 
blood 
from 
AB 
A 
B 
O
Blood 
Group 
Antigens Antibodi 
es 
Can give 
blood to 
Can 
receive 
blood 
from 
AB A and B None AB AB, A, B, 
O 
A A B A and AB A and O 
B B A B and AB B and O 
O None A and B AB, A, B, 
O 
O
Plasma pharesis 
Methods 
Indications 
1- TTP 
2-Cryoglobulinemia 
3- Cutaneous T cell lymphoma 
4-Guillian Barre syndrome 
5-Hyperviscosity syndrome in monoclonal gammopathy 
6-leukostasis 
7-Myasthenia gravis 
8- familial hypercholestrolemia 
9-Goodpasture syndrome
Thank You

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Blood transfusion 2

  • 1. Noha M. El Husseiny, MD Clinical Haematology Unit Cairo University
  • 2.
  • 3. Red blood cell transfusion Whole blood 450ml contains adequate coagulation factors but less platelets. Rarely used nowadays.
  • 4.
  • 5. Fresh Frozen Plasma Preparation from whole blood separated and frozen within 8 hours can be kept up to one year. Volume 200 ml blood To increase factor by 20%need 10-20 ml/kg Properties Expire in 24h after thawing however thawed plasma can be relabelled and used within five days in TTP.
  • 6.
  • 8. Cryoprecipitate The insoluble part of plasma when thawed to 4 C 10-20 ml Properties rich in factor VIII, fibrinogen, factor XIII and VWF (variable concentrate) Correction of hypofibrinogenemia (need 8- 10 units) if fibrinogen 50-100mg/ L
  • 9. Factor concentrates Recombinant factor VIII Recombinant factor VII Factor IX concentrates VWF concentrate
  • 10.
  • 11. Packed red blood cell Preparation are separated from whole blood by centrifugation. Unit is 200ml. Increase HB 1-2 g/ml Indications: transfusion if HB less than 6g/dl Stored up to 6 weeks.
  • 12. Warming of blood components
  • 13.
  • 14.
  • 15. Leuko-reduced packed RBCs Contain less than 107 leukocytes per unit. Used to reduce CMV infection, HLA immunization, Febrile reaction and platelet transfusion refractoriness.
  • 16. Leukoreduced gamma irradiated PRBCs For patients in transplants Washed RBCs using saline to reduce allergic reaction. Frozen RBCs: Add glycerol and freeze RBCs within 6 days of collection used in preserving rare groups for long time than 42 days.
  • 18.
  • 19. Platelet transfusion Preparation may be separated from whole blood or collected by apharesis. Stable for 72 H Indications: Thrombocytopenia. Contraindications TTP, HIT
  • 20. Immunoglobulins Preparation by cold ethanol fractionation of large pool of plasma followed by viral inactivation. Uses treatment of ITP, Guillian Barre syndrome, chronic inflammatory demyelinating polyneuropathy, replacement in immunodeficiency syndrome.
  • 21.
  • 22.
  • 23.
  • 24.
  • 25.
  • 26.
  • 27.
  • 28.
  • 29.
  • 30.
  • 31.
  • 32. Blood Group Antigens Antibodi es Can give blood to Can receive blood from AB A B O
  • 33. Blood Group Antigens Antibodi es Can give blood to Can receive blood from AB A and B None AB AB, A, B, O A A B A and AB A and O B B A B and AB B and O O None A and B AB, A, B, O O
  • 34.
  • 35.
  • 36.
  • 37.
  • 38.
  • 39.
  • 40.
  • 41.
  • 42.
  • 43. Plasma pharesis Methods Indications 1- TTP 2-Cryoglobulinemia 3- Cutaneous T cell lymphoma 4-Guillian Barre syndrome 5-Hyperviscosity syndrome in monoclonal gammopathy 6-leukostasis 7-Myasthenia gravis 8- familial hypercholestrolemia 9-Goodpasture syndrome
  • 44.