Short Note on dried human plasma, thrombin and fibrinogen by Javed

1. Class seminar
On
• Dried human plasma
• Thrombin
• Fibrinogen
Presented by
Javed
B.pharma 3rd year

2. Dried human plasma
It consist about 55 % of blood. This is yellow colour
fluid. It is used for plasma transfusion where medically
needed. It was first developed in British 1930.
Collection
• Take blood from blood donor which want
to donate blood and eligible for donating
blood.
• Separate the blood plasma from
blood by the process of
centrifugation

3. Procedure
Procedure
• Take 400 ml blood plasma which are separated
from blood.
• Packed in to MRC bottle (medical
research council bottle
Freezing-
• The bottle is sealed with
• Bacteriological efficient.
The bottle is then centrifuged at 18°c

4. Primary drying-
• The bottle are mounted horizontally in the drying
chamber at 50°c
• This process takes place about 2 weeks
Secondary drying-
• This is done in another chamber by vaccume
desiccation over phosphorus pentaoxide
• It takes place about a day
• Atleast 0.5% moisture should be left

5. Storage-
• It is kept below 28°c and protected from moisture,
sunlight and remains usable for 5 year8
Uses-
• Blood clotting
• Autoimmune disorders
• hemophilia
• Other medical emergencies

6. Thrombin & fibrinogen
Preparation
• Take citrated plasma (not more than 0.4%)
• Add organic solvent having proper volatility &
bacteriostatic activity,
• A stabilizer such as sodium caprylate.Whose function
is to allow the preparation to be heated for one hour
at low temperature without denaturation of protien.
• For isotonicity Nacl added and to control the
contamination
Both proteins are present in blood plasma these proteins
also used in blood clotting.

7. Mechanism of action
Prothrombin Thrombin
Inactive
Insoluble
Active
Soluble
Fibrinogen
Protein S
Fibrin
(monomer
Fibrous in nature
Insoluble)
Fibrin
Polymers Haemostatis
Blood clotting

9. Thank you