PLASMA Blood products

1. BLOOD PRODUCTS
DIPTI S
B PHARMACY VIII SEMESTER
SRI RAMACHANDRA MEDICALCOLLEGEAND RI
REG NO :3414008

2. BLOOD PLASMA
WHAT IS IT?
 Blood plasma is the liquid component of
whole salts, ions, minerals ,nutrients
,proteins in the solution.
 Plasma accounts for 55 % of whole blood
WHOLE BLOOD= Plasma +WBC + RBC +
Platelets

3. CENTRIFUGATION OF WHOLE
BLOOD

4. FRESH FROZEN PLASMA
 Fresh frozen plasma is isolated from a single donor
and it contains all the coagulation factors
USES :
 Used in deficiency of coagulation factors or any
coagulation problem of blood
 It is also used in poising caused due overdose of
anticoagulants
STORAGE:30 degrees centigrade or colder
conditions .
The used FFP should be compatible with all blood
groups

5. CRYO PRECIPITATE
 This is precipitated plasma containing only
factor VIII obtained from a single unit of
fresh frozen plasma
 USES : Haemophilia A
 STORAGE : -300 degrees centigrade or colder

6. DRY HUMAN PLASMA
 Not used these days due to stability issues
 Store below 20 degree centigrade

7. PLASMA PROTEINS
 The proteins in plasma are known as plasma
proteins they include
Albumin
Immunoglobins
clotting proteins/proteins
 Albumin is the major protein accounting for
60% of the total protein

8. USES OF ALBUMIN
 Maintains osmotic pressure of the blood .
 Serves as a carrier for various enzymes,
hormones and pharmaceutical substances

9. PROPERTIES OF ALBUMIN
 Its solubility in water is the main reason for
this acting as a carrier for water insoluble
/highly lipid soluble drugs
 Has a low iso electric pH( 4.8).This property is
useful in easy precipitation of the protein
 Highly stable when exposed to different
conditions due to the presence of 17
disulphide bonds

10. STRUCTURE OF ALBUMIN

11. USES OF PLASMA
 Increases blood volume in case of trauma
 Plasma has various clotting factors so they
play a major role in the following
VIII- Hemophilia A
IX –Hemophilia B
Fibrinogen – heammorage

12. USE OF IMMUNOGLOBIN
 During Immune deficiency disorders .Example includes Gullien
Barre ‘ syndrome
DIAGNOSTIC USES
 To determine various biochemical disorders using markers
.Generally electrophoresis is carried out.
CLINICAL USE OF ALBUMIN
 Used in hypo albuminia

13. PROCESSING OF PLASMA
 COHN’S METHOD
 In this method the solution of plasma is
taken and the concentration of ethanol is
increased from 0 to 40% at a temperature of
5 degree Centigrade
 Based upon the solubility of the desired
plasma proteins/components are separated .
As he concentration of ethanol increases the
pH should be adjusted ie.. From pH 7 to the
isoelectric pH of the protein

14.  For example the pI of albumin is 4.8. So
therefore at that pH albumin precipitates and
is separated

15. PURIFICATION
 By chromatographic methods purification
takes place
 Ion exchange chromatography is widely used
 PRINCIPLE: Here the proteins /amino
acids are bound to oppositely charged ions
and ionic bonds are created .Based upon the
affinity of the ionized proteins towards the
stationary phase the proteins are attracted
and separated

16. STORAGE
 If the plasma is isolated and is stored for less
than 8 hours then it is known as fresh plasma
 If plasma is stored for more than 8 hours then
it is known as frozen plasma
Temperature for storage of frozen plasma for 1
year -18ºC
Temperature for storage of Plasma for 7 years -
65º C

17. REFERENCES
 Tutorial Pharmacy – Cooper and Gunn
 www.researchgate.net

18. THANKYOU